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P11231 (VP2_ROTHW) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein VP2
OrganismRotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]
Taxonomic identifier10962 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainRepeat
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Inner capsid protein VP2
PRO_0000149533

Regions

Region1 – 100100Interaction with VP1 and VP3 By similarity
Region404 – 42421Hydrophobic By similarity
Region432 – 45221Hydrophobic By similarity
Region546 – 56722Leucine-zipper 1 Potential
Region675 – 69622Leucine-zipper 2 Potential

Sequences

Sequence LengthMass (Da)Tools
P11231 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 0E0933ED56EB4295

FASTA890103,751
        10         20         30         40         50         60 
MAYRKRGAKR ENLPQQNERL QEKEIEKDVD VTMENKNNNR KQQLSDKVLS QKEEIITDAQ 

        70         80         90        100        110        120 
DDIKIAGEIK KSSKEESKQL LEILKTKEDH QKEIQYEILQ KTIPTFESKE SILKKLEDIR 

       130        140        150        160        170        180 
PEQAKKQMKL FRIFEPKQLP IYRANGEKEL RNRWYWKLKK DTLPDGDYDV REYFLNLYDQ 

       190        200        210        220        230        240 
ILIEMPDYLL LKDMAVENKN SRDAGKVVDS ETANICDAIF QDEETEGVVR RFIADMRQQV 

       250        260        270        280        290        300 
QADRNIVNYP SILHPIDHAF NEYFLNHQLV EPLNNEIIFN YIPERIRNDV NYILNMDMNL 

       310        320        330        340        350        360 
PSTARYIRPN LLQDRLNLHD NFESLWDTIT TSNYILARSV VPDLKEKELV STEAQIQKMS 

       370        380        390        400        410        420 
QDLQLEALTI QSETQFLAGI NSQAANDCFK TLIAAMLSQR TMSLDFVTTN YMSLISGMWL 

       430        440        450        460        470        480 
LTVIPNDMFL RESLVACELA IINTIVYPAF GMQRMHYRNG DPQTPFQIAE QQIQNFQVAN 

       490        500        510        520        530        540 
WLHFINNNRF RQVVIDGVLN QTLNDNIRNG QVINQLMEAL MQLSRQQFPT MPVDYKRSIQ 

       550        560        570        580        590        600 
RGILLLSNRL GQLVDLTRLV SYNYETLMAC VTMNMQHVQT LTTEKLQLTS VTSLCMLIGN 

       610        620        630        640        650        660 
TTVIPSPQTL FHYYNINVNF HSNYNERIND AVAIITAANR LNLYQKKMKS IVEDFLKRLQ 

       670        680        690        700        710        720 
IFDVPRVPDD QMYRLRDRLR LLPVERRRLD IFNLILMNME QIERASDKIA QGVIIAYRDM 

       730        740        750        760        770        780 
QLERDEMYGY VNIARNLDGY QQINLEELMR TGDYGQITNM LLNNQPVALV GALPFVTDSS 

       790        800        810        820        830        840 
VISLIAKLDA TVFAQIVKLR KVDTLKPILY KINSDSNDFY LVANYDWIPT STTKVYKQVP 

       850        860        870        880        890 
QPFDFRASMH MLTSNLTFTV YSDLLSFVSA DTVEPINAVA FDNMRIMNEL 

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References

[1]"Molecular characterization of a subgroup specificity associated with the rotavirus inner capsid protein VP2."
McDonald S.M., Patton J.T.
J. Virol. 82:2752-2764(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence of genomic segment 2 of the human rotavirus Wa."
Ernst H., Duhl J.A.
Nucleic Acids Res. 17:4382-4382(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14942 Genomic RNA. Translation: CAA33074.1.
PIRP2XRWA. A34008.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTHW
AccessionPrimary (citable) accession number: P11231
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 16, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families