P11229 (ACM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M1

Gene names

Name:

CHRM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subunit structure	Interacts with GPRASP2. Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Traceable author statement PubMed 1905013. Source: ProtInc cellular protein modification process Traceable author statement PubMed 1411529. Source: ProtInc nervous system development Traceable author statement PubMed 2739737. Source: ProtInc neuromuscular synaptic transmission Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Traceable author statement PubMed 8139539. Source: ProtInc positive regulation of cell proliferation Traceable author statement PubMed 2739737. Source: ProtInc positive regulation of ion transport Inferred from genetic interaction PubMed 14657398. Source: MGI protein kinase C-activating G-protein coupled receptor signaling pathway Traceable author statement PubMed 9603968. Source: ProtInc regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: InterPro regulation of locomotion Inferred from electronic annotation. Source: Compara regulation of phospholipase C activity Inferred from electronic annotation. Source: InterPro
Cellular_component	asymmetric synapse Inferred from electronic annotation. Source: Compara axon terminus Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement PubMed 8508928. Source: ProtInc postsynaptic density Inferred from electronic annotation. Source: Compara postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled acetylcholine receptor activity Traceable author statement PubMed 9614217. Source: ProtInc drug binding Inferred from electronic annotation. Source: Compara phosphatidylinositol phospholipase C activity Traceable author statement PubMed 9614217. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Muscarinic acetylcholine receptor M1		PRO_0000069015
Regions
	Topological domain	1 – 24	24	Extracellular Potential
	Transmembrane	25 – 47	23	Helical; Name=1; By similarity
	Topological domain	48 – 61	14	Cytoplasmic Potential
	Transmembrane	62 – 82	21	Helical; Name=2; By similarity
	Topological domain	83 – 99	17	Extracellular Potential
	Transmembrane	100 – 121	22	Helical; Name=3; By similarity
	Topological domain	122 – 141	20	Cytoplasmic Potential
	Transmembrane	142 – 164	23	Helical; Name=4; By similarity
	Topological domain	165 – 186	22	Extracellular Potential
	Transmembrane	187 – 209	23	Helical; Name=5; By similarity
	Topological domain	210 – 366	157	Cytoplasmic Potential
	Transmembrane	367 – 387	21	Helical; Name=6; By similarity
	Topological domain	388 – 401	14	Extracellular Potential
	Transmembrane	402 – 421	20	Helical; Name=7; By similarity
	Topological domain	422 – 460	39	Cytoplasmic Potential
Amino acid modifications
	Modified residue	428	1	Phosphothreonine Potential
	Modified residue	451	1	Phosphoserine Potential
	Modified residue	455	1	Phosphothreonine Potential
	Modified residue	457	1	Phosphoserine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Probable
	Glycosylation	12	1	N-linked (GlcNAc...) Probable
	Disulfide bond	98 ↔ 178		By similarity
Experimental info
	Sequence conflict	173	1	V → M in CAA33334. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P11229 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: 567C20F63541C8D0	FASTA	460	51,421
	10 20 30 40 50 60 MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 70 80 90 100 110 120 NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 130 140 150 160 170 180 FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 190 200 210 220 230 240 QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 250 260 270 280 290 300 SERSQPGAEG SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 310 320 330 340 350 360 VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA KRKTFSLVKE 370 380 390 400 410 420 KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STINPMCYAL 430 440 450 460 CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the gene encoding the human M1 muscarinic acetylcholine receptor." Allard W.J., Sigal I.S., Dixon R.A.F. Nucleic Acids Res. 15:10604-10604(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation of the human ml (Hml) muscarinic acetylcholine receptor gene by PCR amplification." Chapman C.G., Browne M.J. Nucleic Acids Res. 18:2191-2191(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors." Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J. EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Mutational analysis of third cytoplasmic loop domains in G-protein coupling of the HM1 muscarinic receptor." Arden J.R., Nagata O., Shockley M.S., Philip M., Lameh J., Sadee W. Biochem. Biophys. Res. Commun. 188:1111-1115(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[7]	"Identification of a novel family of G protein-coupled receptor associated sorting proteins." Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L. J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPRASP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00508 Genomic DNA. Translation: CAA68560.1. X52068 Genomic DNA. Translation: CAA36291.1. X15263 Genomic DNA. Translation: CAA33334.1. AF498915 mRNA. Translation: AAM18938.1. BC007740 mRNA. Translation: AAH07740.1. BC022984 mRNA. Translation: AAH22984.1.
IPI	IPI00298980.
PIR	S09508.
RefSeq	NP_000729.2. NM_000738.2.
UniGene	Hs.632119.
3D structure databases
ProteinModelPortal	P11229.
ModBase	Search...
Protein-protein interaction databases
IntAct	P11229. 2 interactions.
MINT	MINT-7970464.
STRING	9606.ENSP00000306490.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P11229.
Polymorphism databases
DMDM	113118.
Proteomic databases
PaxDb	P11229.
PRIDE	P11229.
Protocols and materials databases
DNASU	1128.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306960; ENSP00000306490; ENSG00000168539.
GeneID	1128.
KEGG	hsa:1128.
UCSC	uc001nwi.3. human.
Organism-specific databases
CTD	1128.
GeneCards	GC11M062677.
HGNC	HGNC:1950. CHRM1.
HPA	CAB022366. HPA014101.
MIM	118510. gene.
neXtProt	NX_P11229.
PharmGKB	PA26484.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250863.
HOGENOM	HOG000231484.
HOVERGEN	HBG105720.
InParanoid	P11229.
KO	K04129.
OMA	RCCRTPR.
OrthoDB	EOG4NVZK1.
PhylomeDB	P11229.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P11229.
Bgee	P11229.
CleanEx	HS_CHRM1.
Genevestigator	P11229.
GermOnline	ENSG00000168539. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002228. Musac_Ach_M1_rcpt. IPR000995. Musac_Ach_rcpt. [Graphical view]
PANTHER	PTHR24249:SF59. PTHR24249:SF59. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00538. MUSCRINICM1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P11229.
ChEMBL	CHEMBL216.
DrugBank	DB00517. Anisotropine Methylbromide. DB00572. Atropine. DB00767. Benzquinamide. DB00245. Benztropine. DB01019. Bethanechol. DB00810. Biperiden. DB00354. Buclizine. DB00411. Carbachol. DB00748. Carbinoxamine. DB00185. Cevimeline. DB00771. Clidinium. DB00785. Cryptenamine. DB01176. Cyclizine. DB00979. Cyclopentolate. DB00942. Cycrimine. DB01151. Desipramine. DB00804. Dicyclomine. DB01231. Diphenidol. DB00366. Doxylamine. DB00392. Ethopropazine. DB01148. Flavoxate. DB00986. Glycopyrrolate. DB00725. Homatropine Methylbromide. DB00424. Hyoscyamine. DB00332. Ipratropium. DB00940. Methantheline. DB01403. Methotrimeprazine. DB00462. Methylscopolamine. DB00340. Metixene. DB01233. Metoclopramide. DB00334. Olanzapine. DB01062. Oxybutynin. DB00383. Oxyphencyclimine. DB00219. Oxyphenonium. DB01085. Pilocarpine. DB00670. Pirenzepine. DB00387. Procyclidine. DB00420. Promazine. DB01069. Promethazine. DB00782. Propantheline. DB00777. Propiomazine. DB01103. Quinacrine. DB00747. Scopolamine. DB01591. Solifenacin. DB00202. Succinylcholine. DB00372. Thiethylperazine. DB01036. Tolterodine. DB00505. Tridihexethyl. DB00508. Triflupromazine. DB00376. Trihexyphenidyl. DB00209. Trospium.
GenomeRNAi	1128.
NextBio	4676.
SOURCE	Search...

Entry information

Entry name

ACM1_HUMAN

Accession

Primary (citable) accession number: P11229

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1990
Last modified:	May 29, 2013
This is version 144 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.