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P11229 (ACM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M1
Gene names
Name:CHRM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subunit structure

Interacts with GPRASP2. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled acetylcholine receptor signaling pathway

Traceable author statement PubMed 8139539. Source: ProtInc

G-protein coupled receptor signaling pathway

Traceable author statement PubMed 7925360. Source: ProtInc

cell proliferation

Traceable author statement PubMed 1905013. Source: ProtInc

cellular protein modification process

Traceable author statement PubMed 1411529. Source: ProtInc

cognition

Inferred from electronic annotation. Source: InterPro

nervous system development

Traceable author statement PubMed 2739737. Source: ProtInc

neuromuscular synaptic transmission

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway

Traceable author statement PubMed 8139539. Source: ProtInc

positive regulation of cell proliferation

Traceable author statement PubMed 2739737. Source: ProtInc

positive regulation of intracellular protein transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of ion transport

Inferred from genetic interaction PubMed 14657398. Source: MGI

protein kinase C-activating G-protein coupled receptor signaling pathway

Traceable author statement PubMed 9603968. Source: ProtInc

regulation of locomotion

Inferred from electronic annotation. Source: Ensembl

saliva secretion

Inferred from electronic annotation. Source: InterPro

signal transduction

Traceable author statement Ref.3PubMed 9614217. Source: ProtInc

   Cellular_componentasymmetric synapse

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from electronic annotation. Source: Ensembl

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement PubMed 8508928. Source: ProtInc

membrane

Traceable author statement PubMed 7925360. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled acetylcholine receptor activity

Traceable author statement PubMed 9614217. Source: ProtInc

drug binding

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol phospholipase C activity

Traceable author statement PubMed 9614217. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Muscarinic acetylcholine receptor M1
PRO_0000069015

Regions

Topological domain1 – 2424Extracellular Potential
Transmembrane25 – 4723Helical; Name=1; By similarity
Topological domain48 – 6114Cytoplasmic Potential
Transmembrane62 – 8221Helical; Name=2; By similarity
Topological domain83 – 9917Extracellular Potential
Transmembrane100 – 12122Helical; Name=3; By similarity
Topological domain122 – 14120Cytoplasmic Potential
Transmembrane142 – 16423Helical; Name=4; By similarity
Topological domain165 – 18622Extracellular Potential
Transmembrane187 – 20923Helical; Name=5; By similarity
Topological domain210 – 366157Cytoplasmic Potential
Transmembrane367 – 38721Helical; Name=6; By similarity
Topological domain388 – 40114Extracellular Potential
Transmembrane402 – 42120Helical; Name=7; By similarity
Topological domain422 – 46039Cytoplasmic Potential

Amino acid modifications

Modified residue4281Phosphothreonine Potential
Modified residue4511Phosphoserine Potential
Modified residue4551Phosphothreonine Potential
Modified residue4571Phosphoserine Potential
Glycosylation21N-linked (GlcNAc...) Probable
Glycosylation121N-linked (GlcNAc...) Probable
Disulfide bond98 ↔ 178 By similarity

Experimental info

Sequence conflict1731V → M in CAA33334. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P11229 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: 567C20F63541C8D0

FASTA46051,421
        10         20         30         40         50         60 
MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 

        70         80         90        100        110        120 
NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 

       130        140        150        160        170        180 
FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 

       190        200        210        220        230        240 
QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 

       250        260        270        280        290        300 
SERSQPGAEG SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 

       310        320        330        340        350        360 
VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA KRKTFSLVKE 

       370        380        390        400        410        420 
KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STINPMCYAL 

       430        440        450        460 
CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the gene encoding the human M1 muscarinic acetylcholine receptor."
Allard W.J., Sigal I.S., Dixon R.A.F.
Nucleic Acids Res. 15:10604-10604(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation of the human ml (Hml) muscarinic acetylcholine receptor gene by PCR amplification."
Chapman C.G., Browne M.J.
Nucleic Acids Res. 18:2191-2191(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Mutational analysis of third cytoplasmic loop domains in G-protein coupling of the HM1 muscarinic receptor."
Arden J.R., Nagata O., Shockley M.S., Philip M., Lameh J., Sadee W.
Biochem. Biophys. Res. Commun. 188:1111-1115(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[7]"Identification of a novel family of G protein-coupled receptor associated sorting proteins."
Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPRASP2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00508 Genomic DNA. Translation: CAA68560.1.
X52068 Genomic DNA. Translation: CAA36291.1.
X15263 Genomic DNA. Translation: CAA33334.1.
AF498915 mRNA. Translation: AAM18938.1.
BC007740 mRNA. Translation: AAH07740.1.
BC022984 mRNA. Translation: AAH22984.1.
PIRS09508.
RefSeqNP_000729.2. NM_000738.2.
UniGeneHs.632119.

3D structure databases

ProteinModelPortalP11229.
SMRP11229. Positions 23-434.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107550. 3 interactions.
IntActP11229. 5 interactions.
MINTMINT-7970464.
STRING9606.ENSP00000306490.

Chemistry

BindingDBP11229.
ChEMBLCHEMBL2094109.
DrugBankDB00517. Anisotropine Methylbromide.
DB00572. Atropine.
DB00767. Benzquinamide.
DB00245. Benztropine.
DB01019. Bethanechol.
DB00810. Biperiden.
DB00354. Buclizine.
DB00411. Carbachol.
DB00748. Carbinoxamine.
DB00185. Cevimeline.
DB00771. Clidinium.
DB00785. Cryptenamine.
DB01176. Cyclizine.
DB00979. Cyclopentolate.
DB00942. Cycrimine.
DB01151. Desipramine.
DB00804. Dicyclomine.
DB01231. Diphenidol.
DB00366. Doxylamine.
DB00392. Ethopropazine.
DB01148. Flavoxate.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00332. Ipratropium.
DB00940. Methantheline.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine.
DB00340. Metixene.
DB01233. Metoclopramide.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB00219. Oxyphenonium.
DB01085. Pilocarpine.
DB00670. Pirenzepine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00782. Propantheline.
DB00777. Propiomazine.
DB01103. Quinacrine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB00372. Thiethylperazine.
DB01036. Tolterodine.
DB00505. Tridihexethyl.
DB00508. Triflupromazine.
DB00376. Trihexyphenidyl.
DB00209. Trospium.
GuidetoPHARMACOLOGY13.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP11229.

Polymorphism databases

DMDM113118.

Proteomic databases

PaxDbP11229.
PRIDEP11229.

Protocols and materials databases

DNASU1128.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306960; ENSP00000306490; ENSG00000168539.
GeneID1128.
KEGGhsa:1128.
UCSCuc001nwi.3. human.

Organism-specific databases

CTD1128.
GeneCardsGC11M062695.
HGNCHGNC:1950. CHRM1.
HPACAB022366.
HPA014101.
MIM118510. gene.
neXtProtNX_P11229.
PharmGKBPA26484.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250863.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP11229.
KOK04129.
OMARCCRTPR.
OrthoDBEOG7V49Z7.
PhylomeDBP11229.
TreeFamTF320495.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP11229.
BgeeP11229.
CleanExHS_CHRM1.
GenevestigatorP11229.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF59. PTHR24249:SF59. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMuscarinic_acetylcholine_receptor_M1.
GenomeRNAi1128.
NextBio4676.
PROP11229.
SOURCESearch...

Entry information

Entry nameACM1_HUMAN
AccessionPrimary (citable) accession number: P11229
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries