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P11229

- ACM1_HUMAN

UniProt

P11229 - ACM1_HUMAN

Protein

Muscarinic acetylcholine receptor M1

Gene

CHRM1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 157 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    GO - Molecular functioni

    1. drug binding Source: Ensembl
    2. G-protein coupled acetylcholine receptor activity Source: ProtInc
    3. phosphatidylinositol phospholipase C activity Source: ProtInc

    GO - Biological processi

    1. cell proliferation Source: ProtInc
    2. cellular protein modification process Source: ProtInc
    3. cognition Source: InterPro
    4. G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    5. G-protein coupled receptor signaling pathway Source: ProtInc
    6. nervous system development Source: ProtInc
    7. neuromuscular synaptic transmission Source: Ensembl
    8. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    9. positive regulation of cell proliferation Source: ProtInc
    10. positive regulation of intracellular protein transport Source: Ensembl
    11. positive regulation of ion transport Source: MGI
    12. protein kinase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
    13. regulation of locomotion Source: Ensembl
    14. saliva secretion Source: InterPro
    15. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
    REACT_18283. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M1
    Gene namesi
    Name:CHRM1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1950. CHRM1.

    Subcellular locationi

    GO - Cellular componenti

    1. asymmetric synapse Source: Ensembl
    2. axon terminus Source: Ensembl
    3. cell junction Source: UniProtKB-KW
    4. dendrite Source: Ensembl
    5. integral component of plasma membrane Source: ProtInc
    6. membrane Source: ProtInc
    7. plasma membrane Source: Reactome
    8. postsynaptic density Source: Ensembl
    9. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26484.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Muscarinic acetylcholine receptor M1PRO_0000069015Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Curated
    Glycosylationi12 – 121N-linked (GlcNAc...)Curated
    Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
    Modified residuei428 – 4281PhosphothreonineSequence Analysis
    Modified residuei451 – 4511PhosphoserineSequence Analysis
    Modified residuei455 – 4551PhosphothreonineSequence Analysis
    Modified residuei457 – 4571PhosphoserineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP11229.
    PRIDEiP11229.

    PTM databases

    PhosphoSiteiP11229.

    Expressioni

    Gene expression databases

    ArrayExpressiP11229.
    BgeeiP11229.
    CleanExiHS_CHRM1.
    GenevestigatoriP11229.

    Organism-specific databases

    HPAiCAB022366.
    HPA014101.

    Interactioni

    Subunit structurei

    Interacts with GPRASP2.1 Publication

    Protein-protein interaction databases

    BioGridi107550. 4 interactions.
    IntActiP11229. 5 interactions.
    MINTiMINT-7970464.
    STRINGi9606.ENSP00000306490.

    Structurei

    3D structure databases

    ProteinModelPortaliP11229.
    SMRiP11229. Positions 23-242, 362-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini48 – 6114CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini83 – 9917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini122 – 14120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini165 – 18622ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini210 – 366157CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini388 – 40114ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini422 – 46039CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4723Helical; Name=1By similarityAdd
    BLAST
    Transmembranei62 – 8221Helical; Name=2By similarityAdd
    BLAST
    Transmembranei100 – 12122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei142 – 16423Helical; Name=4By similarityAdd
    BLAST
    Transmembranei187 – 20923Helical; Name=5By similarityAdd
    BLAST
    Transmembranei367 – 38721Helical; Name=6By similarityAdd
    BLAST
    Transmembranei402 – 42120Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiP11229.
    KOiK04129.
    OMAiRCCRTPR.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiP11229.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P11229-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF    50
    KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL 100
    WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW 150
    LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP 200
    VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG 250
    SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 300
    VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA 350
    KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW 400
    ELGYWLCYVN STINPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG 450
    SVHRTPSRQC 460
    Length:460
    Mass (Da):51,421
    Last modified:November 1, 1990 - v2
    Checksum:i567C20F63541C8D0
    GO
    Isoform 2 (identifier: P11229-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         455-460: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:454
    Mass (Da):50,748
    Checksum:i68D02A29898C8D92
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731V → M in CAA33334. (PubMed:3443095)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei455 – 4606Missing in isoform 2. 1 PublicationVSP_056651

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00508 Genomic DNA. Translation: CAA68560.1.
    X52068 Genomic DNA. Translation: CAA36291.1.
    X15263 Genomic DNA. Translation: CAA33334.1.
    AF385587 mRNA. Translation: AAK68112.1.
    AF498915 mRNA. Translation: AAM18938.1.
    AP000438 Genomic DNA. No translation available.
    BC007740 mRNA. Translation: AAH07740.1.
    BC022984 mRNA. Translation: AAH22984.1.
    CCDSiCCDS8040.1.
    PIRiS09508.
    RefSeqiNP_000729.2. NM_000738.2.
    UniGeneiHs.632119.

    Genome annotation databases

    EnsembliENST00000306960; ENSP00000306490; ENSG00000168539.
    ENST00000543973; ENSP00000441188; ENSG00000168539.
    GeneIDi1128.
    KEGGihsa:1128.
    UCSCiuc001nwi.3. human.

    Polymorphism databases

    DMDMi113118.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00508 Genomic DNA. Translation: CAA68560.1 .
    X52068 Genomic DNA. Translation: CAA36291.1 .
    X15263 Genomic DNA. Translation: CAA33334.1 .
    AF385587 mRNA. Translation: AAK68112.1 .
    AF498915 mRNA. Translation: AAM18938.1 .
    AP000438 Genomic DNA. No translation available.
    BC007740 mRNA. Translation: AAH07740.1 .
    BC022984 mRNA. Translation: AAH22984.1 .
    CCDSi CCDS8040.1.
    PIRi S09508.
    RefSeqi NP_000729.2. NM_000738.2.
    UniGenei Hs.632119.

    3D structure databases

    ProteinModelPortali P11229.
    SMRi P11229. Positions 23-242, 362-430.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107550. 4 interactions.
    IntActi P11229. 5 interactions.
    MINTi MINT-7970464.
    STRINGi 9606.ENSP00000306490.

    Chemistry

    BindingDBi P11229.
    ChEMBLi CHEMBL2094109.
    DrugBanki DB00517. Anisotropine Methylbromide.
    DB00572. Atropine.
    DB00767. Benzquinamide.
    DB00245. Benztropine.
    DB01019. Bethanechol.
    DB00810. Biperiden.
    DB00354. Buclizine.
    DB00411. Carbachol.
    DB00748. Carbinoxamine.
    DB00185. Cevimeline.
    DB00771. Clidinium.
    DB00785. Cryptenamine.
    DB01176. Cyclizine.
    DB00979. Cyclopentolate.
    DB00942. Cycrimine.
    DB01151. Desipramine.
    DB00804. Dicyclomine.
    DB01231. Diphenidol.
    DB00366. Doxylamine.
    DB00392. Ethopropazine.
    DB01148. Flavoxate.
    DB00986. Glycopyrrolate.
    DB00725. Homatropine Methylbromide.
    DB00424. Hyoscyamine.
    DB00332. Ipratropium.
    DB00940. Methantheline.
    DB01403. Methotrimeprazine.
    DB00462. Methylscopolamine.
    DB00340. Metixene.
    DB01233. Metoclopramide.
    DB00334. Olanzapine.
    DB01062. Oxybutynin.
    DB00383. Oxyphencyclimine.
    DB00219. Oxyphenonium.
    DB01085. Pilocarpine.
    DB00670. Pirenzepine.
    DB00387. Procyclidine.
    DB00420. Promazine.
    DB01069. Promethazine.
    DB00782. Propantheline.
    DB00777. Propiomazine.
    DB01103. Quinacrine.
    DB00747. Scopolamine.
    DB01591. Solifenacin.
    DB00202. Succinylcholine.
    DB00372. Thiethylperazine.
    DB01036. Tolterodine.
    DB00505. Tridihexethyl.
    DB00508. Triflupromazine.
    DB00376. Trihexyphenidyl.
    DB00209. Trospium.
    GuidetoPHARMACOLOGYi 13.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P11229.

    Polymorphism databases

    DMDMi 113118.

    Proteomic databases

    PaxDbi P11229.
    PRIDEi P11229.

    Protocols and materials databases

    DNASUi 1128.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000306960 ; ENSP00000306490 ; ENSG00000168539 .
    ENST00000543973 ; ENSP00000441188 ; ENSG00000168539 .
    GeneIDi 1128.
    KEGGi hsa:1128.
    UCSCi uc001nwi.3. human.

    Organism-specific databases

    CTDi 1128.
    GeneCardsi GC11M062695.
    HGNCi HGNC:1950. CHRM1.
    HPAi CAB022366.
    HPA014101.
    MIMi 118510. gene.
    neXtProti NX_P11229.
    PharmGKBi PA26484.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250863.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi P11229.
    KOi K04129.
    OMAi RCCRTPR.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi P11229.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_16943. Muscarinic acetylcholine receptors.
    REACT_18283. G alpha (q) signalling events.

    Miscellaneous databases

    GeneWikii Muscarinic_acetylcholine_receptor_M1.
    GenomeRNAii 1128.
    NextBioi 4676.
    PROi P11229.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P11229.
    Bgeei P11229.
    CleanExi HS_CHRM1.
    Genevestigatori P11229.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the gene encoding the human M1 muscarinic acetylcholine receptor."
      Allard W.J., Sigal I.S., Dixon R.A.F.
      Nucleic Acids Res. 15:10604-10604(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Isolation of the human ml (Hml) muscarinic acetylcholine receptor gene by PCR amplification."
      Chapman C.G., Browne M.J.
      Nucleic Acids Res. 18:2191-2191(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
      Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
      EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning Cholinergic Receptors in Human Keratinocytes."
      Arredondo J., Grando S.A.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    8. "Mutational analysis of third cytoplasmic loop domains in G-protein coupling of the HM1 muscarinic receptor."
      Arden J.R., Nagata O., Shockley M.S., Philip M., Lameh J., Sadee W.
      Biochem. Biophys. Res. Commun. 188:1111-1115(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    9. "Identification of a novel family of G protein-coupled receptor associated sorting proteins."
      Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
      J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GPRASP2.

    Entry informationi

    Entry nameiACM1_HUMAN
    AccessioniPrimary (citable) accession number: P11229
    Secondary accession number(s): Q96RH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 157 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3