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Protein

Muscarinic acetylcholine receptor M1

Gene

CHRM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

  • drug binding Source: Ensembl
  • G-protein coupled acetylcholine receptor activity Source: ProtInc
  • phosphatidylinositol phospholipase C activity Source: ProtInc

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • cell proliferation Source: ProtInc
  • cellular protein modification process Source: ProtInc
  • cognition Source: InterPro
  • G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: ProtInc
  • nervous system development Source: ProtInc
  • neuromuscular synaptic transmission Source: Ensembl
  • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  • positive regulation of cell proliferation Source: ProtInc
  • positive regulation of intracellular protein transport Source: Ensembl
  • positive regulation of ion transport Source: MGI
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
  • regulation of locomotion Source: Ensembl
  • regulation of vascular smooth muscle contraction Source: GO_Central
  • saliva secretion Source: InterPro
  • signal transduction Source: ProtInc
  • synaptic transmission, cholinergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168539-MONOMER.
ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M1
Gene namesi
Name:CHRM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:1950. CHRM1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24ExtracellularSequence analysisAdd BLAST24
Transmembranei25 – 47Helical; Name=1By similarityAdd BLAST23
Topological domaini48 – 61CytoplasmicSequence analysisAdd BLAST14
Transmembranei62 – 82Helical; Name=2By similarityAdd BLAST21
Topological domaini83 – 99ExtracellularSequence analysisAdd BLAST17
Transmembranei100 – 121Helical; Name=3By similarityAdd BLAST22
Topological domaini122 – 141CytoplasmicSequence analysisAdd BLAST20
Transmembranei142 – 164Helical; Name=4By similarityAdd BLAST23
Topological domaini165 – 186ExtracellularSequence analysisAdd BLAST22
Transmembranei187 – 209Helical; Name=5By similarityAdd BLAST23
Topological domaini210 – 366CytoplasmicSequence analysisAdd BLAST157
Transmembranei367 – 387Helical; Name=6By similarityAdd BLAST21
Topological domaini388 – 401ExtracellularSequence analysisAdd BLAST14
Transmembranei402 – 421Helical; Name=7By similarityAdd BLAST20
Topological domaini422 – 460CytoplasmicSequence analysisAdd BLAST39

GO - Cellular componenti

  • asymmetric synapse Source: Ensembl
  • axon terminus Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • dendrite Source: Ensembl
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • postsynaptic density Source: Ensembl
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1128.
OpenTargetsiENSG00000168539.
PharmGKBiPA26484.

Chemistry databases

ChEMBLiCHEMBL216.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00245. Benzatropine.
DB00810. Biperiden.
DB00835. Brompheniramine.
DB00354. Buclizine.
DB00411. Carbachol.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00215. Citalopram.
DB00771. Clidinium.
DB00363. Clozapine.
DB00907. Cocaine.
DB00785. Cryptenamine.
DB00979. Cyclopentolate.
DB00942. Cycrimine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00804. Dicyclomine.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB00366. Doxylamine.
DB01175. Escitalopram.
DB00392. Ethopropazine.
DB06702. Fesoterodine.
DB01148. Flavoxate.
DB00875. Flupentixol.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB00940. Methantheline.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01233. Metoclopramide.
DB00805. Minaprine.
DB01618. Molindone.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB00219. Oxyphenonium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB00670. Pirenzepine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00782. Propantheline.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00508. Triflupromazine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00209. Trospium.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi13.

Polymorphism and mutation databases

BioMutaiCHRM1.
DMDMi113118.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690151 – 460Muscarinic acetylcholine receptor M1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...)Curated1
Glycosylationi12N-linked (GlcNAc...)Curated1
Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
Modified residuei230PhosphothreonineBy similarity1
Modified residuei428PhosphothreonineSequence analysis1
Modified residuei451PhosphoserineSequence analysis1
Modified residuei455PhosphothreonineSequence analysis1
Modified residuei457PhosphoserineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP11229.
PeptideAtlasiP11229.
PRIDEiP11229.

PTM databases

iPTMnetiP11229.
PhosphoSitePlusiP11229.

Expressioni

Gene expression databases

BgeeiENSG00000168539.
CleanExiHS_CHRM1.
ExpressionAtlasiP11229. baseline and differential.
GenevisibleiP11229. HS.

Organism-specific databases

HPAiCAB022366.
HPA014101.

Interactioni

Subunit structurei

Interacts with GPRASP2.1 Publication

Protein-protein interaction databases

BioGridi107550. 4 interactors.
IntActiP11229. 5 interactors.
MINTiMINT-7970464.
STRINGi9606.ENSP00000306490.

Chemistry databases

BindingDBiP11229.

Structurei

Secondary structure

1460
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 52Combined sources31
Helixi54 – 56Combined sources3
Helixi59 – 76Combined sources18
Helixi78 – 88Combined sources11
Helixi95 – 128Combined sources34
Turni130 – 133Combined sources4
Helixi134 – 136Combined sources3
Helixi139 – 168Combined sources30
Helixi186 – 196Combined sources11
Helixi198 – 212Combined sources15
Helixi215 – 218Combined sources4
Helixi358 – 390Combined sources33
Beta strandi391 – 393Combined sources3
Helixi397 – 406Combined sources10
Helixi409 – 421Combined sources13
Helixi425 – 433Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CXVX-ray2.70A2-218[»]
A355-460[»]
ProteinModelPortaliP11229.
SMRiP11229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP11229.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG091G06VI.
PhylomeDBiP11229.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11229-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF
60 70 80 90 100
KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL
110 120 130 140 150
WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW
160 170 180 190 200
LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP
210 220 230 240 250
VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
260 270 280 290 300
SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
310 320 330 340 350
VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA
360 370 380 390 400
KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW
410 420 430 440 450
ELGYWLCYVN STINPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG
460
SVHRTPSRQC
Length:460
Mass (Da):51,421
Last modified:November 1, 1990 - v2
Checksum:i567C20F63541C8D0
GO
Isoform 2 (identifier: P11229-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-460: Missing.

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):50,748
Checksum:i68D02A29898C8D92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173V → M in CAA33334 (PubMed:3443095).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056651455 – 460Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00508 Genomic DNA. Translation: CAA68560.1.
X52068 Genomic DNA. Translation: CAA36291.1.
X15263 Genomic DNA. Translation: CAA33334.1.
AF385587 mRNA. Translation: AAK68112.1.
AF498915 mRNA. Translation: AAM18938.1.
AP000438 Genomic DNA. No translation available.
BC007740 mRNA. Translation: AAH07740.1.
BC022984 mRNA. Translation: AAH22984.1.
CCDSiCCDS8040.1. [P11229-1]
PIRiS09508.
RefSeqiNP_000729.2. NM_000738.2. [P11229-1]
XP_011543044.1. XM_011544742.2. [P11229-1]
UniGeneiHs.632119.

Genome annotation databases

EnsembliENST00000306960; ENSP00000306490; ENSG00000168539. [P11229-1]
ENST00000543973; ENSP00000441188; ENSG00000168539. [P11229-2]
GeneIDi1128.
KEGGihsa:1128.
UCSCiuc058cqg.1. human. [P11229-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00508 Genomic DNA. Translation: CAA68560.1.
X52068 Genomic DNA. Translation: CAA36291.1.
X15263 Genomic DNA. Translation: CAA33334.1.
AF385587 mRNA. Translation: AAK68112.1.
AF498915 mRNA. Translation: AAM18938.1.
AP000438 Genomic DNA. No translation available.
BC007740 mRNA. Translation: AAH07740.1.
BC022984 mRNA. Translation: AAH22984.1.
CCDSiCCDS8040.1. [P11229-1]
PIRiS09508.
RefSeqiNP_000729.2. NM_000738.2. [P11229-1]
XP_011543044.1. XM_011544742.2. [P11229-1]
UniGeneiHs.632119.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CXVX-ray2.70A2-218[»]
A355-460[»]
ProteinModelPortaliP11229.
SMRiP11229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107550. 4 interactors.
IntActiP11229. 5 interactors.
MINTiMINT-7970464.
STRINGi9606.ENSP00000306490.

Chemistry databases

BindingDBiP11229.
ChEMBLiCHEMBL216.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00245. Benzatropine.
DB00810. Biperiden.
DB00835. Brompheniramine.
DB00354. Buclizine.
DB00411. Carbachol.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00215. Citalopram.
DB00771. Clidinium.
DB00363. Clozapine.
DB00907. Cocaine.
DB00785. Cryptenamine.
DB00979. Cyclopentolate.
DB00942. Cycrimine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00804. Dicyclomine.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB00366. Doxylamine.
DB01175. Escitalopram.
DB00392. Ethopropazine.
DB06702. Fesoterodine.
DB01148. Flavoxate.
DB00875. Flupentixol.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB00940. Methantheline.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01233. Metoclopramide.
DB00805. Minaprine.
DB01618. Molindone.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB00219. Oxyphenonium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB00670. Pirenzepine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00782. Propantheline.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00508. Triflupromazine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00209. Trospium.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi13.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP11229.
PhosphoSitePlusiP11229.

Polymorphism and mutation databases

BioMutaiCHRM1.
DMDMi113118.

Proteomic databases

PaxDbiP11229.
PeptideAtlasiP11229.
PRIDEiP11229.

Protocols and materials databases

DNASUi1128.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306960; ENSP00000306490; ENSG00000168539. [P11229-1]
ENST00000543973; ENSP00000441188; ENSG00000168539. [P11229-2]
GeneIDi1128.
KEGGihsa:1128.
UCSCiuc058cqg.1. human. [P11229-1]

Organism-specific databases

CTDi1128.
DisGeNETi1128.
GeneCardsiCHRM1.
HGNCiHGNC:1950. CHRM1.
HPAiCAB022366.
HPA014101.
MIMi118510. gene.
neXtProtiNX_P11229.
OpenTargetsiENSG00000168539.
PharmGKBiPA26484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP11229.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG091G06VI.
PhylomeDBiP11229.
TreeFamiTF320495.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168539-MONOMER.
ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-416476. G alpha (q) signalling events.

Miscellaneous databases

GeneWikiiMuscarinic_acetylcholine_receptor_M1.
GenomeRNAii1128.
PROiP11229.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168539.
CleanExiHS_CHRM1.
ExpressionAtlasiP11229. baseline and differential.
GenevisibleiP11229. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM1_HUMAN
AccessioniPrimary (citable) accession number: P11229
Secondary accession number(s): Q96RH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.