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Protein

Muscarinic acetylcholine receptor M1

Gene

CHRM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

  1. drug binding Source: Ensembl
  2. G-protein coupled acetylcholine receptor activity Source: ProtInc
  3. phosphatidylinositol phospholipase C activity Source: ProtInc

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  2. cell proliferation Source: ProtInc
  3. cellular protein modification process Source: ProtInc
  4. cognition Source: InterPro
  5. G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  6. G-protein coupled receptor signaling pathway Source: ProtInc
  7. nervous system development Source: ProtInc
  8. neuromuscular synaptic transmission Source: Ensembl
  9. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  10. positive regulation of cell proliferation Source: ProtInc
  11. positive regulation of intracellular protein transport Source: Ensembl
  12. positive regulation of ion transport Source: MGI
  13. protein kinase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
  14. regulation of locomotion Source: Ensembl
  15. saliva secretion Source: InterPro
  16. sensory perception of chemical stimulus Source: GO_Central
  17. signal transduction Source: ProtInc
  18. synaptic transmission, cholinergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_18283. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M1
Gene namesi
Name:CHRM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:1950. CHRM1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
BLAST
Transmembranei25 – 4723Helical; Name=1By similarityAdd
BLAST
Topological domaini48 – 6114CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei62 – 8221Helical; Name=2By similarityAdd
BLAST
Topological domaini83 – 9917ExtracellularSequence AnalysisAdd
BLAST
Transmembranei100 – 12122Helical; Name=3By similarityAdd
BLAST
Topological domaini122 – 14120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei142 – 16423Helical; Name=4By similarityAdd
BLAST
Topological domaini165 – 18622ExtracellularSequence AnalysisAdd
BLAST
Transmembranei187 – 20923Helical; Name=5By similarityAdd
BLAST
Topological domaini210 – 366157CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei367 – 38721Helical; Name=6By similarityAdd
BLAST
Topological domaini388 – 40114ExtracellularSequence AnalysisAdd
BLAST
Transmembranei402 – 42120Helical; Name=7By similarityAdd
BLAST
Topological domaini422 – 46039CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. asymmetric synapse Source: Ensembl
  2. axon terminus Source: Ensembl
  3. cell junction Source: UniProtKB-KW
  4. dendrite Source: Ensembl
  5. integral component of plasma membrane Source: ProtInc
  6. membrane Source: ProtInc
  7. plasma membrane Source: Reactome
  8. postsynaptic density Source: Ensembl
  9. postsynaptic membrane Source: UniProtKB-SubCell
  10. synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26484.

Chemistry

DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00245. Benzatropine.
DB00810. Biperiden.
DB00835. Brompheniramine.
DB00354. Buclizine.
DB00411. Carbachol.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00215. Citalopram.
DB00771. Clidinium.
DB00363. Clozapine.
DB00907. Cocaine.
DB00785. Cryptenamine.
DB00979. Cyclopentolate.
DB00942. Cycrimine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00804. Dicyclomine.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB00366. Doxylamine.
DB01175. Escitalopram.
DB00392. Ethopropazine.
DB06702. Fesoterodine.
DB01148. Flavoxate.
DB00875. Flupentixol.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB00940. Methantheline.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01233. Metoclopramide.
DB00805. Minaprine.
DB01618. Molindone.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB00219. Oxyphenonium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB00670. Pirenzepine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00782. Propantheline.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00508. Triflupromazine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00209. Trospium.
DB00246. Ziprasidone.

Polymorphism and mutation databases

BioMutaiCHRM1.
DMDMi113118.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Muscarinic acetylcholine receptor M1PRO_0000069015Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Curated
Glycosylationi12 – 121N-linked (GlcNAc...)Curated
Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
Modified residuei428 – 4281PhosphothreonineSequence Analysis
Modified residuei451 – 4511PhosphoserineSequence Analysis
Modified residuei455 – 4551PhosphothreonineSequence Analysis
Modified residuei457 – 4571PhosphoserineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP11229.
PRIDEiP11229.

PTM databases

PhosphoSiteiP11229.

Expressioni

Gene expression databases

BgeeiP11229.
CleanExiHS_CHRM1.
ExpressionAtlasiP11229. baseline and differential.
GenevestigatoriP11229.

Organism-specific databases

HPAiCAB022366.
HPA014101.

Interactioni

Subunit structurei

Interacts with GPRASP2.1 Publication

Protein-protein interaction databases

BioGridi107550. 4 interactions.
IntActiP11229. 5 interactions.
MINTiMINT-7970464.
STRINGi9606.ENSP00000306490.

Structurei

3D structure databases

ProteinModelPortaliP11229.
SMRiP11229. Positions 23-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP11229.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG7V49Z7.
PhylomeDBiP11229.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF216. PTHR24249:SF216. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11229-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF
60 70 80 90 100
KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL
110 120 130 140 150
WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW
160 170 180 190 200
LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP
210 220 230 240 250
VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
260 270 280 290 300
SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
310 320 330 340 350
VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA
360 370 380 390 400
KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW
410 420 430 440 450
ELGYWLCYVN STINPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG
460
SVHRTPSRQC
Length:460
Mass (Da):51,421
Last modified:November 1, 1990 - v2
Checksum:i567C20F63541C8D0
GO
Isoform 2 (identifier: P11229-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-460: Missing.

Note: No experimental confirmation available.

Show »
Length:454
Mass (Da):50,748
Checksum:i68D02A29898C8D92
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731V → M in CAA33334 (PubMed:3443095).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei455 – 4606Missing in isoform 2. 1 PublicationVSP_056651

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00508 Genomic DNA. Translation: CAA68560.1.
X52068 Genomic DNA. Translation: CAA36291.1.
X15263 Genomic DNA. Translation: CAA33334.1.
AF385587 mRNA. Translation: AAK68112.1.
AF498915 mRNA. Translation: AAM18938.1.
AP000438 Genomic DNA. No translation available.
BC007740 mRNA. Translation: AAH07740.1.
BC022984 mRNA. Translation: AAH22984.1.
CCDSiCCDS8040.1. [P11229-1]
PIRiS09508.
RefSeqiNP_000729.2. NM_000738.2. [P11229-1]
UniGeneiHs.632119.

Genome annotation databases

EnsembliENST00000306960; ENSP00000306490; ENSG00000168539. [P11229-1]
ENST00000543973; ENSP00000441188; ENSG00000168539. [P11229-2]
GeneIDi1128.
KEGGihsa:1128.
UCSCiuc001nwi.3. human. [P11229-1]

Polymorphism and mutation databases

BioMutaiCHRM1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00508 Genomic DNA. Translation: CAA68560.1.
X52068 Genomic DNA. Translation: CAA36291.1.
X15263 Genomic DNA. Translation: CAA33334.1.
AF385587 mRNA. Translation: AAK68112.1.
AF498915 mRNA. Translation: AAM18938.1.
AP000438 Genomic DNA. No translation available.
BC007740 mRNA. Translation: AAH07740.1.
BC022984 mRNA. Translation: AAH22984.1.
CCDSiCCDS8040.1. [P11229-1]
PIRiS09508.
RefSeqiNP_000729.2. NM_000738.2. [P11229-1]
UniGeneiHs.632119.

3D structure databases

ProteinModelPortaliP11229.
SMRiP11229. Positions 23-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107550. 4 interactions.
IntActiP11229. 5 interactions.
MINTiMINT-7970464.
STRINGi9606.ENSP00000306490.

Chemistry

BindingDBiP11229.
ChEMBLiCHEMBL2111418.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00245. Benzatropine.
DB00810. Biperiden.
DB00835. Brompheniramine.
DB00354. Buclizine.
DB00411. Carbachol.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00215. Citalopram.
DB00771. Clidinium.
DB00363. Clozapine.
DB00907. Cocaine.
DB00785. Cryptenamine.
DB00979. Cyclopentolate.
DB00942. Cycrimine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00804. Dicyclomine.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB00366. Doxylamine.
DB01175. Escitalopram.
DB00392. Ethopropazine.
DB06702. Fesoterodine.
DB01148. Flavoxate.
DB00875. Flupentixol.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB00940. Methantheline.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01233. Metoclopramide.
DB00805. Minaprine.
DB01618. Molindone.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB00219. Oxyphenonium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB00670. Pirenzepine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00782. Propantheline.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00508. Triflupromazine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00209. Trospium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi13.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP11229.

Polymorphism and mutation databases

BioMutaiCHRM1.
DMDMi113118.

Proteomic databases

PaxDbiP11229.
PRIDEiP11229.

Protocols and materials databases

DNASUi1128.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306960; ENSP00000306490; ENSG00000168539. [P11229-1]
ENST00000543973; ENSP00000441188; ENSG00000168539. [P11229-2]
GeneIDi1128.
KEGGihsa:1128.
UCSCiuc001nwi.3. human. [P11229-1]

Organism-specific databases

CTDi1128.
GeneCardsiGC11M062714.
HGNCiHGNC:1950. CHRM1.
HPAiCAB022366.
HPA014101.
MIMi118510. gene.
neXtProtiNX_P11229.
PharmGKBiPA26484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP11229.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG7V49Z7.
PhylomeDBiP11229.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_18283. G alpha (q) signalling events.

Miscellaneous databases

GeneWikiiMuscarinic_acetylcholine_receptor_M1.
GenomeRNAii1128.
NextBioi4676.
PROiP11229.
SOURCEiSearch...

Gene expression databases

BgeeiP11229.
CleanExiHS_CHRM1.
ExpressionAtlasiP11229. baseline and differential.
GenevestigatoriP11229.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF216. PTHR24249:SF216. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the gene encoding the human M1 muscarinic acetylcholine receptor."
    Allard W.J., Sigal I.S., Dixon R.A.F.
    Nucleic Acids Res. 15:10604-10604(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation of the human ml (Hml) muscarinic acetylcholine receptor gene by PCR amplification."
    Chapman C.G., Browne M.J.
    Nucleic Acids Res. 18:2191-2191(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
    Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
    EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning Cholinergic Receptors in Human Keratinocytes."
    Arredondo J., Grando S.A.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  8. "Mutational analysis of third cytoplasmic loop domains in G-protein coupling of the HM1 muscarinic receptor."
    Arden J.R., Nagata O., Shockley M.S., Philip M., Lameh J., Sadee W.
    Biochem. Biophys. Res. Commun. 188:1111-1115(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  9. "Identification of a novel family of G protein-coupled receptor associated sorting proteins."
    Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
    J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPRASP2.

Entry informationi

Entry nameiACM1_HUMAN
AccessioniPrimary (citable) accession number: P11229
Secondary accession number(s): Q96RH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: April 29, 2015
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.