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Protein

Muscarinic acetylcholine receptor M1

Gene

CHRM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • cell proliferation Source: ProtInc
  • cellular protein modification process Source: ProtInc
  • cognition Source: InterPro
  • G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: Reactome
  • nervous system development Source: ProtInc
  • neuromuscular synaptic transmission Source: Ensembl
  • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  • positive regulation of cell proliferation Source: ProtInc
  • positive regulation of intracellular protein transport Source: Ensembl
  • positive regulation of ion transport Source: MGI
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
  • regulation of locomotion Source: Ensembl
  • regulation of vascular smooth muscle contraction Source: GO_Central
  • saliva secretion Source: InterPro
  • signal transduction Source: ProtInc
  • synaptic transmission, cholinergic Source: GO_Central

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-390648 Muscarinic acetylcholine receptors
R-HSA-416476 G alpha (q) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M1
Gene namesi
Name:CHRM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000168539.3
HGNCiHGNC:1950 CHRM1
MIMi118510 gene
neXtProtiNX_P11229

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24ExtracellularSequence analysisAdd BLAST24
Transmembranei25 – 47Helical; Name=1By similarityAdd BLAST23
Topological domaini48 – 61CytoplasmicSequence analysisAdd BLAST14
Transmembranei62 – 82Helical; Name=2By similarityAdd BLAST21
Topological domaini83 – 99ExtracellularSequence analysisAdd BLAST17
Transmembranei100 – 121Helical; Name=3By similarityAdd BLAST22
Topological domaini122 – 141CytoplasmicSequence analysisAdd BLAST20
Transmembranei142 – 164Helical; Name=4By similarityAdd BLAST23
Topological domaini165 – 186ExtracellularSequence analysisAdd BLAST22
Transmembranei187 – 209Helical; Name=5By similarityAdd BLAST23
Topological domaini210 – 366CytoplasmicSequence analysisAdd BLAST157
Transmembranei367 – 387Helical; Name=6By similarityAdd BLAST21
Topological domaini388 – 401ExtracellularSequence analysisAdd BLAST14
Transmembranei402 – 421Helical; Name=7By similarityAdd BLAST20
Topological domaini422 – 460CytoplasmicSequence analysisAdd BLAST39

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1128
OpenTargetsiENSG00000168539
PharmGKBiPA26484

Chemistry databases

ChEMBLiCHEMBL216
DrugBankiDB08897 Aclidinium
DB05766 ACP-104
DB05752 ALKS 27
DB00321 Amitriptyline
DB00543 Amoxapine
DB00517 Anisotropine Methylbromide
DB04365 Arecoline
DB01238 Aripiprazole
DB00572 Atropine
DB00245 Benzatropine
DB00767 Benzquinamide
DB00810 Biperiden
DB00835 Brompheniramine
DB00354 Buclizine
DB00411 Carbachol
DB00185 Cevimeline
DB00477 Chlorpromazine
DB01239 Chlorprothixene
DB00568 Cinnarizine
DB00215 Citalopram
DB00771 Clidinium
DB00363 Clozapine
DB00907 Cocaine
DB00785 Cryptenamine
DB00979 Cyclopentolate
DB00942 Cycrimine
DB00434 Cyproheptadine
DB00496 Darifenacin
DB01151 Desipramine
DB00804 Dicyclomine
DB01231 Diphenidol
DB00280 Disopyramide
DB01142 Doxepin
DB00366 Doxylamine
DB01175 Escitalopram
DB00392 Ethopropazine
DB06702 Fesoterodine
DB01148 Flavoxate
DB00875 Flupentixol
DB00986 Glycopyrronium
DB00725 Homatropine Methylbromide
DB00424 Hyoscyamine
DB00458 Imipramine
DB00332 Ipratropium bromide
DB01221 Ketamine
DB00408 Loxapine
DB00934 Maprotiline
DB04843 Mepenzolate
DB00940 Methantheline
DB01403 Methotrimeprazine
DB00462 Methylscopolamine bromide
DB00340 Metixene
DB01233 Metoclopramide
DB00805 Minaprine
DB01618 Molindone
DB05152 NGX267
DB00622 Nicardipine
DB00540 Nortriptyline
DB00334 Olanzapine
DB01062 Oxybutynin
DB00383 Oxyphencyclimine
DB00219 Oxyphenonium
DB00715 Paroxetine
DB00454 Pethidine
DB01085 Pilocarpine
DB00670 Pirenzepine
DB00387 Procyclidine
DB00420 Promazine
DB01069 Promethazine
DB00782 Propantheline
DB00777 Propiomazine
DB01224 Quetiapine
DB00747 Scopolamine
DB01591 Solifenacin
DB00202 Succinylcholine
DB00342 Terfenadine
DB01409 Tiotropium
DB01036 Tolterodine
DB00505 Tridihexethyl
DB00508 Triflupromazine
DB00376 Trihexyphenidyl
DB00726 Trimipramine
DB00809 Tropicamide
DB00209 Trospium
DB09076 Umeclidinium
DB00246 Ziprasidone
GuidetoPHARMACOLOGYi13

Polymorphism and mutation databases

BioMutaiCHRM1
DMDMi113118

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690151 – 460Muscarinic acetylcholine receptor M1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineCurated1
Glycosylationi12N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
Modified residuei230PhosphothreonineBy similarity1
Modified residuei428PhosphothreonineSequence analysis1
Modified residuei451PhosphoserineSequence analysis1
Modified residuei455PhosphothreonineSequence analysis1
Modified residuei457PhosphoserineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP11229
PeptideAtlasiP11229
PRIDEiP11229

PTM databases

iPTMnetiP11229
PhosphoSitePlusiP11229

Expressioni

Gene expression databases

BgeeiENSG00000168539
CleanExiHS_CHRM1
ExpressionAtlasiP11229 baseline and differential
GenevisibleiP11229 HS

Organism-specific databases

HPAiCAB022366
HPA014101

Interactioni

Subunit structurei

Interacts with GPRASP2.1 Publication

Protein-protein interaction databases

BioGridi107550, 3 interactors
IntActiP11229, 5 interactors
MINTiP11229
STRINGi9606.ENSP00000306490

Chemistry databases

BindingDBiP11229

Structurei

Secondary structure

1460
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 52Combined sources31
Helixi54 – 56Combined sources3
Helixi59 – 76Combined sources18
Helixi78 – 88Combined sources11
Helixi95 – 128Combined sources34
Turni130 – 133Combined sources4
Helixi134 – 136Combined sources3
Helixi139 – 168Combined sources30
Helixi186 – 196Combined sources11
Helixi198 – 212Combined sources15
Helixi215 – 218Combined sources4
Helixi358 – 390Combined sources33
Beta strandi391 – 393Combined sources3
Helixi397 – 406Combined sources10
Helixi409 – 421Combined sources13
Helixi425 – 433Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CXVX-ray2.70A2-218[»]
A355-460[»]
ProteinModelPortaliP11229
SMRiP11229
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP11229
KOiK04129
OMAiRCCRTPR
OrthoDBiEOG091G06VI
PhylomeDBiP11229
TreeFamiTF320495

Family and domain databases

CDDicd15049 7tmA_mAChR, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR002228 Musac_Ach_M1_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF7 PTHR43914:SF7, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00538 MUSCRINICM1R
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11229-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF
60 70 80 90 100
KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL
110 120 130 140 150
WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW
160 170 180 190 200
LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP
210 220 230 240 250
VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
260 270 280 290 300
SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
310 320 330 340 350
VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA
360 370 380 390 400
KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW
410 420 430 440 450
ELGYWLCYVN STINPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG
460
SVHRTPSRQC
Length:460
Mass (Da):51,421
Last modified:November 1, 1990 - v2
Checksum:i567C20F63541C8D0
GO
Isoform 2 (identifier: P11229-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-460: Missing.

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):50,748
Checksum:i68D02A29898C8D92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173V → M in CAA33334 (PubMed:3443095).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056651455 – 460Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00508 Genomic DNA Translation: CAA68560.1
X52068 Genomic DNA Translation: CAA36291.1
X15263 Genomic DNA Translation: CAA33334.1
AF385587 mRNA Translation: AAK68112.1
AF498915 mRNA Translation: AAM18938.1
AP000438 Genomic DNA No translation available.
BC007740 mRNA Translation: AAH07740.1
BC022984 mRNA Translation: AAH22984.1
CCDSiCCDS8040.1 [P11229-1]
PIRiS09508
RefSeqiNP_000729.2, NM_000738.2 [P11229-1]
XP_011543044.1, XM_011544742.2 [P11229-1]
UniGeneiHs.632119

Genome annotation databases

EnsembliENST00000306960; ENSP00000306490; ENSG00000168539 [P11229-1]
ENST00000543973; ENSP00000441188; ENSG00000168539 [P11229-2]
GeneIDi1128
KEGGihsa:1128
UCSCiuc058cqg.1 human [P11229-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACM1_HUMAN
AccessioniPrimary (citable) accession number: P11229
Secondary accession number(s): Q96RH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: February 28, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health