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P11227 (POL_MLVRD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pol polyprotein

Cleaved into the following 3 chains:

  1. Protease
    EC=3.4.23.-
  2. Reverse transcriptase/ribonuclease H
    Short name=RT
    EC=2.7.7.49
    EC=3.1.26.4
  3. Integrase
    Short name=IN
Gene names
Name:pol
OrganismRadiation murine leukemia virus [Complete proteome]
Taxonomic identifier11787 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length1196 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins.

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Sequence similarities

Belongs to the retroviral Pol polyprotein family.

Contains 1 integrase catalytic domain.

Contains 1 peptidase A2 domain.

Contains 1 reverse transcriptase domain.

Contains 1 RNase H domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11961196Pol polyprotein
PRO_0000259739
Chain1 – 103103Protease
PRO_0000026134
Chain104 – ?Reverse transcriptase/ribonuclease HPRO_0000259740
Chain? – 1196IntegrasePRO_0000259741

Regions

Domain22 – 9271Peptidase A2
Domain202 – 393192Reverse transcriptase
Domain635 – 781147RNase H
Domain905 – 1063159Integrase catalytic

Sites

Active site271 By similarity

Sequences

Sequence LengthMass (Da)Tools
P11227 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 9549253FCEEB5306

FASTA1,196133,323
        10         20         30         40         50         60 
GGQGQEPPPE PRITLKVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ GATGGKRYRW 

        70         80         90        100        110        120 
TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI HFKGSGAQIV GPMGQPLQVL 

       130        140        150        160        170        180 
TLNIEDEYRL HEISTEPDVS PGSTWLSDFP QAWAETGGMG LAVRQAPLII PLKATSTPVS 

       190        200        210        220        230        240 
IKQYPMSQEA KLGIKPHIQR LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQGLREVNK 

       250        260        270        280        290        300 
RVEDIHPTVP NPYNLLSGLP TSHRWYTVLD LKDAFFCLRL HPTSQPLFAS EWRDPGMGIS 

       310        320        330        340        350        360 
GQLTWTRLPQ GFKNSPTLFD EALHRGLADF RIQHPDLILL QYVDDLLLAA TSELDCQQGT 

       370        380        390        400        410        420 
RALLKTLGNL GYRASAKKAQ ICQKQVKYLG YLLREGQRWL TEARKETVMG QPTPKTPRQL 

       430        440        450        460        470        480 
REFLGTAGFC RLWIPRFAEM AAPLYPLTKT GTLFNWGPDQ QKAYHEIKQA LLTAPALGLP 

       490        500        510        520        530        540 
DLTKPFELFV DEKQGYAKGV LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLT 

       550        560        570        580        590        600 
KDAGKLTMGQ PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 

       610        620        630        640        650        660 
ATLLPLPEEG APHDCLEILA ETHGTEPDLT DQPIPDADHT WYTDGSSFLQ EGQRKAGAAV 

       670        680        690        700        710        720 
TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV YTDSRYAFAT AHIHGEIYKR 

       730        740        750        760        770        780 
RGLLTSEGRE IKNKSEILAL LKALFLPKRL SIIHCLGHQK GDSAEARGNR LADQAAREAA 

       790        800        810        820        830        840 
IKTPPDTSTL LIEDSTPYTP AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV 

       850        860        870        880        890        900 
FELLDSLHRL THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 

       910        920        930        940        950        960 
GVRVRGHRPG THWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKHET AKIVTKKLLE 

       970        980        990       1000       1010       1020 
EIFPRFGMPQ VLGTDNGPAF VSQVSQSVAK LLGIDWKLHC AYRPQSSGQV ERMNRTIKET 

      1030       1040       1050       1060       1070       1080 
LTKLTLATGT RDWVLLLPLA LYRARNTPGP HGLTPYEILY GAPPPLVNFH DPEMSKFTNS 

      1090       1100       1110       1120       1130       1140 
PSLQAHLQAL QAVQREVWKP LAAAYQDQLD QPVIPHPFRV GDTVWVRRHQ TKNLEPRWKG 

      1150       1160       1170       1180       1190 
PYTVLLTTPT ALKVDGISAW IHAAHVKAAT TPPIRPSWRV QRSQNPLKIR LTRGAP 

« Hide

References

[1]"Nucleotide sequence of a radiation leukemia virus genome."
Merregaert J., Janowski M., Reddy E.P.
Virology 158:88-102(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K03363 Genomic RNA. No translation available.
PIRGNMVRV. B26183.

3D structure databases

ProteinModelPortalP11227.
SMRP11227. Positions 144-594, 623-788.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOL_MLVRD
AccessionPrimary (citable) accession number: P11227
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries