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P11227

- POL_MLVRD

UniProt

P11227 - POL_MLVRD

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Protein

Pol polyprotein

Gene

pol

Organism
Radiation murine leukemia virus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei27 – 271PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. establishment of integrated proviral latency Source: UniProtKB-KW
  4. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:pol
OrganismiRadiation murine leukemia virus
Taxonomic identifieri11787 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007778: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1196IntegrasePRO_0000259741
Chaini1 – 11961196Pol polyproteinPRO_0000259739Add
BLAST
Chaini1 – 103103ProteasePRO_0000026134Add
BLAST
Chaini104 – ?Reverse transcriptase/ribonuclease HPRO_0000259740

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

3D structure databases

ProteinModelPortaliP11227.
SMRiP11227. Positions 144-594, 623-788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 9271Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini202 – 393192Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini635 – 781147RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini905 – 1063159Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11227-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
GGQGQEPPPE PRITLKVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ
60 70 80 90 100
GATGGKRYRW TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI
110 120 130 140 150
HFKGSGAQIV GPMGQPLQVL TLNIEDEYRL HEISTEPDVS PGSTWLSDFP
160 170 180 190 200
QAWAETGGMG LAVRQAPLII PLKATSTPVS IKQYPMSQEA KLGIKPHIQR
210 220 230 240 250
LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQGLREVNK RVEDIHPTVP
260 270 280 290 300
NPYNLLSGLP TSHRWYTVLD LKDAFFCLRL HPTSQPLFAS EWRDPGMGIS
310 320 330 340 350
GQLTWTRLPQ GFKNSPTLFD EALHRGLADF RIQHPDLILL QYVDDLLLAA
360 370 380 390 400
TSELDCQQGT RALLKTLGNL GYRASAKKAQ ICQKQVKYLG YLLREGQRWL
410 420 430 440 450
TEARKETVMG QPTPKTPRQL REFLGTAGFC RLWIPRFAEM AAPLYPLTKT
460 470 480 490 500
GTLFNWGPDQ QKAYHEIKQA LLTAPALGLP DLTKPFELFV DEKQGYAKGV
510 520 530 540 550
LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLT KDAGKLTMGQ
560 570 580 590 600
PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP
610 620 630 640 650
ATLLPLPEEG APHDCLEILA ETHGTEPDLT DQPIPDADHT WYTDGSSFLQ
660 670 680 690 700
EGQRKAGAAV TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV
710 720 730 740 750
YTDSRYAFAT AHIHGEIYKR RGLLTSEGRE IKNKSEILAL LKALFLPKRL
760 770 780 790 800
SIIHCLGHQK GDSAEARGNR LADQAAREAA IKTPPDTSTL LIEDSTPYTP
810 820 830 840 850
AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV FELLDSLHRL
860 870 880 890 900
THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA
910 920 930 940 950
GVRVRGHRPG THWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKHET
960 970 980 990 1000
AKIVTKKLLE EIFPRFGMPQ VLGTDNGPAF VSQVSQSVAK LLGIDWKLHC
1010 1020 1030 1040 1050
AYRPQSSGQV ERMNRTIKET LTKLTLATGT RDWVLLLPLA LYRARNTPGP
1060 1070 1080 1090 1100
HGLTPYEILY GAPPPLVNFH DPEMSKFTNS PSLQAHLQAL QAVQREVWKP
1110 1120 1130 1140 1150
LAAAYQDQLD QPVIPHPFRV GDTVWVRRHQ TKNLEPRWKG PYTVLLTTPT
1160 1170 1180 1190
ALKVDGISAW IHAAHVKAAT TPPIRPSWRV QRSQNPLKIR LTRGAP
Length:1,196
Mass (Da):133,323
Last modified:July 1, 1989 - v1
Checksum:i9549253FCEEB5306
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K03363 Genomic RNA. No translation available.
PIRiB26183. GNMVRV.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K03363 Genomic RNA. No translation available.
PIRi B26183. GNMVRV.

3D structure databases

ProteinModelPortali P11227.
SMRi P11227. Positions 144-594, 623-788.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view ]
Pfami PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view ]
SUPFAMi SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a radiation leukemia virus genome."
    Merregaert J., Janowski M., Reddy E.P.
    Virology 158:88-102(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOL_MLVRD
AccessioniPrimary (citable) accession number: P11227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3