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P11227

- POL_MLVRD

UniProt

P11227 - POL_MLVRD

Protein

Pol polyprotein

Gene

pol

Organism
Radiation murine leukemia virus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei27 – 271PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. DNA recombination Source: UniProtKB-KW
    3. establishment of integrated proviral latency Source: UniProtKB-KW
    4. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Keywords - Biological processi

    DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pol polyprotein
    Cleaved into the following 3 chains:
    Gene namesi
    Name:pol
    OrganismiRadiation murine leukemia virus
    Taxonomic identifieri11787 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000007778: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1196IntegrasePRO_0000259741
    Chaini1 – 11961196Pol polyproteinPRO_0000259739Add
    BLAST
    Chaini1 – 103103ProteasePRO_0000026134Add
    BLAST
    Chaini104 – ?Reverse transcriptase/ribonuclease HPRO_0000259740

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins.

    Structurei

    3D structure databases

    ProteinModelPortaliP11227.
    SMRiP11227. Positions 144-594, 623-788.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 9271Peptidase A2PROSITE-ProRule annotationAdd
    BLAST
    Domaini202 – 393192Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST
    Domaini635 – 781147RNase HPROSITE-ProRule annotationAdd
    BLAST
    Domaini905 – 1063159Integrase catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the retroviral Pol polyprotein family.Curated
    Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
    Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
    Contains 1 RNase H domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProiIPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view]
    PfamiPF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11227-1 [UniParc]FASTAAdd to Basket

    « Hide

    GGQGQEPPPE PRITLKVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ     50
    GATGGKRYRW TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI 100
    HFKGSGAQIV GPMGQPLQVL TLNIEDEYRL HEISTEPDVS PGSTWLSDFP 150
    QAWAETGGMG LAVRQAPLII PLKATSTPVS IKQYPMSQEA KLGIKPHIQR 200
    LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQGLREVNK RVEDIHPTVP 250
    NPYNLLSGLP TSHRWYTVLD LKDAFFCLRL HPTSQPLFAS EWRDPGMGIS 300
    GQLTWTRLPQ GFKNSPTLFD EALHRGLADF RIQHPDLILL QYVDDLLLAA 350
    TSELDCQQGT RALLKTLGNL GYRASAKKAQ ICQKQVKYLG YLLREGQRWL 400
    TEARKETVMG QPTPKTPRQL REFLGTAGFC RLWIPRFAEM AAPLYPLTKT 450
    GTLFNWGPDQ QKAYHEIKQA LLTAPALGLP DLTKPFELFV DEKQGYAKGV 500
    LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLT KDAGKLTMGQ 550
    PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 600
    ATLLPLPEEG APHDCLEILA ETHGTEPDLT DQPIPDADHT WYTDGSSFLQ 650
    EGQRKAGAAV TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV 700
    YTDSRYAFAT AHIHGEIYKR RGLLTSEGRE IKNKSEILAL LKALFLPKRL 750
    SIIHCLGHQK GDSAEARGNR LADQAAREAA IKTPPDTSTL LIEDSTPYTP 800
    AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV FELLDSLHRL 850
    THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 900
    GVRVRGHRPG THWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKHET 950
    AKIVTKKLLE EIFPRFGMPQ VLGTDNGPAF VSQVSQSVAK LLGIDWKLHC 1000
    AYRPQSSGQV ERMNRTIKET LTKLTLATGT RDWVLLLPLA LYRARNTPGP 1050
    HGLTPYEILY GAPPPLVNFH DPEMSKFTNS PSLQAHLQAL QAVQREVWKP 1100
    LAAAYQDQLD QPVIPHPFRV GDTVWVRRHQ TKNLEPRWKG PYTVLLTTPT 1150
    ALKVDGISAW IHAAHVKAAT TPPIRPSWRV QRSQNPLKIR LTRGAP 1196
    Length:1,196
    Mass (Da):133,323
    Last modified:July 1, 1989 - v1
    Checksum:i9549253FCEEB5306
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03363 Genomic RNA. No translation available.
    PIRiB26183. GNMVRV.

    Keywords - Coding sequence diversityi

    RNA suppression of termination

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03363 Genomic RNA. No translation available.
    PIRi B26183. GNMVRV.

    3D structure databases

    ProteinModelPortali P11227.
    SMRi P11227. Positions 144-594, 623-788.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProi IPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view ]
    Pfami PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a radiation leukemia virus genome."
      Merregaert J., Janowski M., Reddy E.P.
      Virology 158:88-102(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPOL_MLVRD
    AccessioniPrimary (citable) accession number: P11227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is synthesized as a Gag-Pol polyprotein.

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3