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Protein

Spike glycoprotein

Gene

S

Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.By similarity
S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoprotein
Short name:
S glycoprotein
Alternative name(s):
E2
Peplomer protein
Cleaved into the following 2 chains:
Alternative name(s):
90B
Alternative name(s):
90A
Gene namesi
Name:S
ORF Names:3
OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifieri11144 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000007193 Componenti: Genome

Subcellular locationi

Spike protein S2 :
Spike protein S1 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini11 – 11741164ExtracellularSequence analysisAdd
BLAST
Transmembranei1175 – 119723HelicalSequence analysisAdd
BLAST
Topological domaini1198 – 123538CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1010Sequence analysis
Chaini11 – 12351225Spike glycoproteinPRO_0000037220Add
BLAST
Chaini11 – 628618Spike protein S1By similarityPRO_0000037221Add
BLAST
Chaini629 – 1235607Spike protein S2By similarityPRO_0000037222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi31 – 311N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi60 – 601N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi192 – 1921N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi357 – 3571N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi435 – 4351N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi536 – 5361N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi568 – 5681N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi576 – 5761N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi599 – 5991N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi648 – 6481N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi665 – 6651N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi804 – 8041N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1091 – 10911N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1101 – 11011N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1120 – 11201N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1136 – 11361N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1157 – 11571N-linked (GlcNAc...); by hostSequence analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R. Cleavage is not necessary for virus-cell fusion, and fusion probably occurs at the host plasma membrane (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei628 – 6292Cleavage; by hostBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes. S1 interacts with murine CEACAM1, and weakly with murine CEACAM2 in tissue culture (By similarity).By similarity

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZSVX-ray1.80E/F457-464[»]
2ZSWX-ray2.80M/N/O/P457-464[»]
ProteinModelPortaliP11225.
SMRiP11225. Positions 875-951, 1127-1165.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11225.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 330330Receptor binding siteAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili910 – 95445Sequence analysisAdd
BLAST
Coiled coili1138 – 116629Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1198 – 121518Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the coronaviruses spike protein family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.790. 1 hit.
InterProiIPR002552. Corona_S2.
IPR027400. S_HR2.
IPR032500. Spike_N.
IPR018548. Spike_rcpt-bd.
[Graphical view]
PfamiPF01601. Corona_S2. 1 hit.
PF16451. Spike_NTD. 1 hit.
PF09408. Spike_rec_bind. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFVFILLLP SCLGYIGDFR CIQTVNYNGN NASAPSISTE AVDVSKGRGT
60 70 80 90 100
YYVLDRVYLN ATLLLTGYYP VDGSNYRNLA LTGTNTLSLT WFKPPFLSEF
110 120 130 140 150
NDGIFAKVQN LKTNTPTGAT SYFPTIVIGS LFGNTSYTVV LEPYNNIIMA
160 170 180 190 200
SVCTYTICQL PYTPCKPNTN GNRVIGFWHT DVKPPICLLK RNFTFNVNAP
210 220 230 240 250
WLYFHFYQQG GTFYAYYADK PSATTFLFSV YIGDILTQYF VLPFICTPTA
260 270 280 290 300
GSTLAPLYWV TPLLKRQYLF NFNEKGVITS AVDCASSYIS EIKCKTQSLL
310 320 330 340 350
PSTGVYDLSG YTVQPVGVVY RRVPNLPDCK IEEWLTAKSV PSPLNWERRT
360 370 380 390 400
FQNCNFNLSS LLRYVQAESL SCNNIDASKV YGMCFGSVSV DKFAIPRSRQ
410 420 430 440 450
IDLQIGNSGF LQTANYKIDT AATSCQLYYS LPKNNVTINN YNPSSWNRRY
460 470 480 490 500
GFKVNDRCQI FANILLNGIN SGTTCSTDLQ LPNTEVATGV CVRYDLYGIT
510 520 530 540 550
GQGVFKEVKA DYYNSWQALL YDVNGNLNGF RDLTTNKTYT IRSCYSGRVS
560 570 580 590 600
AAYHKEAPEP ALLYRNINCS YVFTNNISRE ENPLNYFDSY LGCVVNADNR
610 620 630 640 650
TDEALPNCNL RMGAGLCVDY SKSRRARRSV STGYRLTTFE PYMPMLVNDS
660 670 680 690 700
VQSVGGLYEM QIPTNFTIGH HEEFIQIRAP KVTIDCAAFV CGDNAACRQQ
710 720 730 740 750
LVEYGSFCDN VNAILNEVNN LLDNMQLQVA SALMQGVTIS SRLPDGISGP
760 770 780 790 800
IDDINFSPLL GCIGSTCAED GNGPSAIRGR SAIEDLLFDK VKLSDVGFVE
810 820 830 840 850
AYNNCTGGQE VRDLLCVQSF NGIKVLPPVL SESQISGYTA GATAAAMFPP
860 870 880 890 900
WTAAAGVPFS LNVQYRINGL GVTMNVLSEN QKMIASAFNN ALGAIQEGFD
910 920 930 940 950
ATNSALGKIQ SVVNANAEAL NNLLNQLSNR FGAISASLQE ILTRLDAVEA
960 970 980 990 1000
KAQIDRLING RLTALNAYIS KQLSDSTLIK FSAAQAIEKV NECVKSQTTR
1010 1020 1030 1040 1050
INFCGNGNHI LSLVQNAPYG LCFIHFSYVP TSFKTANVSP GLCISGDRGL
1060 1070 1080 1090 1100
APKAGYFVQD NGEWKFTGSN YYYPEPITDK NSVAMISCAV NYTKAPEVFL
1110 1120 1130 1140 1150
NNSIPNLPDF KEELDKWFKN QTSIAPDLSL DFEKLNVTFL DLTYEMNRIQ
1160 1170 1180 1190 1200
DAIKKLNESY INLKEVGTYE MYVKWPWYVW LLIGLAGVAV CVLLFFICCC
1210 1220 1230
TGCGSCCFRK CGSCCDEYGG HQDSIVIHNI SAHED
Length:1,235
Mass (Da):136,654
Last modified:July 1, 1989 - v1
Checksum:i25962AD6C1F92DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04797 mRNA. Translation: CAA28484.1.
PIRiA33095. VGIHMJ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04797 mRNA. Translation: CAA28484.1.
PIRiA33095. VGIHMJ.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZSVX-ray1.80E/F457-464[»]
2ZSWX-ray2.80M/N/O/P457-464[»]
ProteinModelPortaliP11225.
SMRiP11225. Positions 875-951, 1127-1165.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP11225.

Family and domain databases

Gene3Di1.20.5.790. 1 hit.
InterProiIPR002552. Corona_S2.
IPR027400. S_HR2.
IPR032500. Spike_N.
IPR018548. Spike_rcpt-bd.
[Graphical view]
PfamiPF01601. Corona_S2. 1 hit.
PF16451. Spike_NTD. 1 hit.
PF09408. Spike_rec_bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the gene encoding the surface projection glycoprotein of coronavirus MHV-JHM."
    Schmidt I., Skinner M.A., Siddell S.G.
    J. Gen. Virol. 68:47-56(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Bgp2, a new member of the carcinoembryonic antigen-related gene family, encodes an alternative receptor for mouse hepatitis viruses."
    Nedellec P., Dveksler G.S., Daniels E., Turbide C., Chow B., Basile A.A., Holmes K.V., Beauchemin N.
    J. Virol. 68:4525-4537(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MURINE CEACAM2.

Entry informationi

Entry nameiSPIKE_CVMJH
AccessioniPrimary (citable) accession number: P11225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 9, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The viral fusion process requires lipid rafts. Spike glycoprotein presumably interacts with target cell lipid raft after cell attachment (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.