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P11207

- V_SV5

UniProt

P11207 - V_SV5

Protein

Non-structural protein V

Gene

P/V

Organism
Simian virus 5 (strain W3) (SV5)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Plays an essential role in the inhibition of host immune response. Prevents the establishment of cellular antiviral state by blocking interferon-alpha/beta (IFN-alpha/beta) production and signaling pathway. Interacts with host IFIH1/MDA5 and DHX58/LGP2 to inhibit the transduction pathway involved in the activation of IFN-beta promoter, thus protecting the virus against cell antiviral state. Efficiently blocks type I IFN signaling following infection by behaving as a substrate receptor for CUL4-DDB1 E3 ligase complex and targeting host STAT1 for proteasomal degradation.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi171 – 1711Zinc 1
    Metal bindingi190 – 1901Zinc 1
    Metal bindingi194 – 1941Zinc 2
    Metal bindingi206 – 2061Zinc 2
    Metal bindingi208 – 2081Zinc 2
    Metal bindingi211 – 2111Zinc 2
    Metal bindingi215 – 2151Zinc 1
    Metal bindingi218 – 2181Zinc 1

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host MDA-5 activity Source: CACAO
    2. suppression by virus of host STAT1 activity Source: UniProtKB-KW
    3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host MDA5 by virus, Inhibition of host RLR pathway by virus, Inhibition of host STAT1 by virus, Interferon antiviral system evasion, Ubl conjugation pathway, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein V
    Gene namesi
    Name:P/V
    OrganismiSimian virus 5 (strain W3) (SV5)
    Taxonomic identifieri11208 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRubulavirus
    Virus hostiCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]
    Homo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007232: Genome

    Subcellular locationi

    Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Non-structural protein VPRO_0000142830Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host DDB1, STAT2 and IFIH1/MDA5.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DDB1Q165313EBI-6148694,EBI-350322From a different organism.
    DHX58Q96C102EBI-6148694,EBI-744193From a different organism.
    IFIH1Q9BYX42EBI-6148694,EBI-6115771From a different organism.
    STAT2P526302EBI-6148694,EBI-1546963From a different organism.

    Protein-protein interaction databases

    DIPiDIP-48759N.
    IntActiP11207. 6 interactions.
    MINTiMINT-6623137.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 3210
    Beta strandi95 – 973
    Turni101 – 1044
    Beta strandi109 – 1168
    Beta strandi119 – 1213
    Helixi127 – 13812
    Beta strandi143 – 1464
    Beta strandi164 – 1674
    Beta strandi170 – 18011
    Beta strandi183 – 19210
    Beta strandi202 – 2043
    Beta strandi207 – 2093
    Helixi216 – 2183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B5LX-ray2.85C/D1-222[»]
    2HYEX-ray3.10B1-222[»]
    2K48NMR-A95-108[»]
    4I1SX-ray2.29B168-219[»]
    ProteinModelPortaliP11207.
    SMRiP11207. Positions 10-222.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11207.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the paramyxoviruses V protein family.Curated

    Family and domain databases

    InterProiIPR024279. Paramyx_V_Zn-bd.
    IPR025909. Soyouz_module.
    [Graphical view]
    PfamiPF14313. Soyouz_module. 1 hit.
    PF13008. zf-Paramyx-P. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11207-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPTDLSFSP DEINKLIETG LNTVEYFTSQ QVTGTSSLGK NTIPPGVTGL    50
    LTNAAEAKIQ ESTNHQKGSV GGGAKPKKPR PKIAIVPADD KTVPGKPIPN 100
    PLLGLDSTPS TQTVLDLSGK TLPSGSYKGV KLAKFGKENL MTRFIEEPRE 150
    NPIATSSPID FKRGRDTGGF HRREYSIGWV GDEVKVTEWC NPSCSPITAA 200
    ARRFECTCHQ CPVTCSECER DT 222
    Length:222
    Mass (Da):23,935
    Last modified:July 1, 1989 - v1
    Checksum:i13D2F1627D15AFA3
    GO

    RNA editingi

    Partially edited. RNA editing at this position consists of an insertion of two guanine nucleotides. The sequence displayed here is the V protein, derived from the unedited RNA. The edited RNA gives rise to the P protein (AC P11208).

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03142 mRNA. Translation: AAA47882.1.
    AF052755 Genomic RNA. Translation: AAC95512.1.
    PIRiA31594. MNNZSP.
    RefSeqiYP_138513.1. NC_006430.1.

    Genome annotation databases

    GeneIDi3160800.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03142 mRNA. Translation: AAA47882.1 .
    AF052755 Genomic RNA. Translation: AAC95512.1 .
    PIRi A31594. MNNZSP.
    RefSeqi YP_138513.1. NC_006430.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B5L X-ray 2.85 C/D 1-222 [» ]
    2HYE X-ray 3.10 B 1-222 [» ]
    2K48 NMR - A 95-108 [» ]
    4I1S X-ray 2.29 B 168-219 [» ]
    ProteinModelPortali P11207.
    SMRi P11207. Positions 10-222.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48759N.
    IntActi P11207. 6 interactions.
    MINTi MINT-6623137.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3160800.

    Miscellaneous databases

    EvolutionaryTracei P11207.

    Family and domain databases

    InterProi IPR024279. Paramyx_V_Zn-bd.
    IPR025909. Soyouz_module.
    [Graphical view ]
    Pfami PF14313. Soyouz_module. 1 hit.
    PF13008. zf-Paramyx-P. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5."
      Thomas S.M., Lamb R.A., Paterson R.G.
      Cell 54:891-902(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING.
    2. "NP:P and NP:V interactions of the paramyxovirus simian virus 5 examined using a novel protein:protein capture assay."
      Randall R.E., Bermingham A.
      Virology 224:121-129(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PROTEIN NP.
    3. "The RNA binding region of the paramyxovirus SV5 V and P proteins."
      Lin G.Y., Paterson R.G., Lamb R.A.
      Virology 238:460-469(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING.
    4. "The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter."
      Andrejeva J., Childs K.S., Young D.F., Carlos T.S., Stock N., Goodbourn S., Randall R.E.
      Proc. Natl. Acad. Sci. U.S.A. 101:17264-17269(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN IFIH1/MDA5, INTERFERON EVASION.
    5. "In vitro and in vivo specificity of ubiquitination and degradation of STAT1 and STAT2 by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2."
      Precious B., Young D.F., Andrejeva L., Goodbourn S., Randall R.E.
      J. Gen. Virol. 86:151-158(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION OF HUMAN STAT1 AND HUMAN STAT2.
    6. "The role of simian virus 5 V protein on viral RNA synthesis."
      Lin Y., Horvath F., Aligo J.A., Wilson R., He B.
      Virology 338:270-280(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Simian virus 5 V protein acts as an adaptor, linking DDB1 to STAT2, to facilitate the ubiquitination of STAT1."
      Precious B., Childs K., Fitzpatrick-Swallow V., Goodbourn S., Randall R.E.
      J. Virol. 79:13434-13441(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN DDB1 AND HUMAN STAT2.
    8. "Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction."
      Childs K., Randall R., Goodbourn S.
      J. Virol. 86:3411-3421(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST DHX58.
    9. "Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase."
      Li T., Chen X., Garbutt K.C., Zhou P., Zheng N.
      Cell 124:105-117(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 1-222, FUNCTION.
    10. "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery."
      Angers S., Li T., Yi X., MacCoss M.J., Moon R.T., Zheng N.
      Nature 443:590-593(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH HUMAN CUL4A; DDB1 AND ROC1, FUNCTION.

    Entry informationi

    Entry nameiV_SV5
    AccessioniPrimary (citable) accession number: P11207
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3