Reviewed, UniProtKB/Swiss-Prot P11205 (L_NDVB)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Newcastle disease virus (strain Beaudette C/45) (NDV)

Taxonomic identifier

11178 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Avulavirus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length	2204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxoviruses L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2204	2204	Large structural protein		PRO_0000142731
Regions
	Domain	634 – 818	185	RdRp catalytic
	Nucleotide binding	1775 – 1784	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P11205-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: C67B8674D904802C	FASTA	2,204	248,824
	10 20 30 40 50 60 MASSGPERAE HQIILPESHL SSPLVKHKLL YYWKLTGLPL PDECDFDHLI LSRQWKKILE 70 80 90 100 110 120 SASPDTERMI KLGRAVHQTL NHNSRITGVL HPRCLEELAS IEVPDSTNKF RKIEKKIQIH 130 140 150 160 170 180 NTRYGELFTR LCTHIEKKLL GSSWSNNVPR SEEFNSIRTD PAFWFHSKWS TAKFAWLHIK 190 200 210 220 230 240 QIQRHLIVAA RTRSAANKLV MLTHKVGQVF VTPELVIVTH TNENKFTCLT QELVLMYADM 250 260 270 280 290 300 MEGRDMVNII STTAVHLRSL SEKIDDILQL IDALAKDLGN QVYDVVSLME GFAYGAVQLL 310 320 330 340 350 360 EPSGRFAGHF FAFNLQELKD ILIGLLPNDI AESVTHAIAT VFSGLEQNQA AEMLCLLRLW 370 380 390 400 410 420 GHPLLESRIA AKAVRSQMCA PKMVDFDMIL QVLSFFKGTI INGYRKKNAG VWPRVKVDTI 430 440 450 460 470 480 YGKIIGQLHA DSAEISHDIM LREYKSLSAL EFEPCIEYDP VTNLSMFLKD KAIAHPNDNW 490 500 510 520 530 540 LASFRRNLLS EDQKKHVKEA TSTNRLLIEF LESNDFDPYK EMEYLTTLEY LRDDDVAVSY 550 560 570 580 590 600 SLKEKEVKVN GRIFAKLTKK LRNCQVMAEG ILADQIAPFF QGNGVIQDSI SLTKSTLAMS 610 620 630 640 650 660 QLSFNSNKKR ITDCKERVSS NRNHDPKSKN RRRVATFITT DLQKYCLNWR YQTIKLFAHA 670 680 690 700 710 720 INQLMGLPHF FEWIHLRLMD TTMFVGDPFN PPSDPTDCDL SRVPNDDIYI VSARGGIEGL 730 740 750 760 770 780 CQKLWTMISI AAIQLAAARS HCRVACMVQG DNQVIAVTRE VRSDDSPEMV LTQLHQASDN 790 800 810 820 830 840 FFKELIHVNH LIGHNLKDRE TIRSDTFFIY SKRIFKDGAI LSQVLKNSSK LVMVSGDLSE 850 860 870 880 890 900 NTVMSCANIA STVARLCENG LPKDFCYYLN YIMSCVQTYF DSEFSYNNNS HPDLNQSWIE 910 920 930 940 950 960 DISFVHSYVL TPAQLGGLSN LQYSRLYTRN IGDPGTTAFA EIKRLEAVGL LSPNIMTNIL 970 980 990 1000 1010 1020 TRPPGNGDWA SLCNDPYSFN FETVASPNIV LKKHTQRVLF ETCSNPLLSG VHTEDNEAEE 1030 1040 1050 1060 1070 1080 KALAEFLLNQ EVIHPRVAHA IMEASSVGRR KQIQGLVDTT NTVIKIALTR RPLGIKRLMR 1090 1100 1110 1120 1130 1140 IVNYSSMHAM LFRDDVFSSN RSNHPLVSSN MCSLTLADYA RNRSWSPLTG GRKILGVSNP 1150 1160 1170 1180 1190 1200 DTIELVEGEI LSVSGGCTRC DSGDEQFTWF HLPSNIELTD DTSKNPPMRV PYLGSKTQER 1210 1220 1230 1240 1250 1260 RAASLAKIAH MSPHVKAALR ASSVLIWAYG DNEVNWTAAL TIAKSRCNIN LEYLRLLSPL 1270 1280 1290 1300 1310 1320 PTAGNLQHRL DDGITQMTFT PASLYRCHLT FTYPMILKGY SLKKESKRGM WFINRVMLLG 1330 1340 1350 1360 1370 1380 LSLIESIFPM TTTRTYDEIT LHLHSKFSCC IREAPVAVPF ELLGVAPELR TVTSNKFMYD 1390 1400 1410 1420 1430 1440 PSPVSEGDFA RLDLAIFKSY ELNLESYPTI ELMNILSISS GKLIGQSVVS YDEDTSIKND 1450 1460 1470 1480 1490 1500 AIIVYDNTRN WISEAQNSDV VRLFEYAALE VLLDCSYQLY YLRVRGLDNI VLYMGDLYKN 1510 1520 1530 1540 1550 1560 MPGILLSNIA ATISHPVIHS RLHAVGLVNH NGSHQLADTD FIEMSAKLLV SCTRRVISGL 1570 1580 1590 1600 1610 1620 YSGNKYDLLF PSVLDDNLNE KMLQLISRLC CLYTVLFATT REIPKIRGLS AEEKCSVLTE 1630 1640 1650 1660 1670 1680 YLLSDAVKPL LSPDQVSSIM SPNIITFPAN LYYMSRKSLN LIREREDKDS ILALLFPQEP 1690 1700 1710 1720 1730 1740 LLEFPSVQDI GARVKDPFTR QPAAFLQELD LSAPARYDAF TLSQIHPELT SPNPEEDYLV 1750 1760 1770 1780 1790 1800 RYLFRGIGTA SSSWYKASHL LSVPEVRCAR HGNSLYLAEG SGAIMSLLEL HVPHETIYYN 1810 1820 1830 1840 1850 1860 TLFSNEMNPP QRHFGPTPTQ FLNSVVYRNL QAEVTCKDGF VQEFRPLWRE NTEESDLTSD 1870 1880 1890 1900 1910 1920 KAVGYITSAV PYRSVSLLHC DIEIPPGSNQ SLLDQLAINL SLIAMHSVRE GGVVIIKVLY 1930 1940 1950 1960 1970 1980 AMGYYFHLLM NLFAPCSTKG YILSNGYACR GDMECYLVFV MGYLGGPTFV HEVVRMAKTL 1990 2000 2010 2020 2030 2040 VQRHGTLLSK SDEITLTRLF TSQRQRVTDI LSSPLPRLIK YLRKNIDTAL IEAGGQPVRP 2050 2060 2070 2080 2090 2100 FCAESLVSTL ADITQITQII ASHIDTVIRS VIYMEAEGDL ADTVFLFTPY NLSTDGKKRT 2110 2120 2130 2140 2150 2160 SLKQCTRQIL EVTILGLRVE DLNKIGDVIS LVLKGMISME DLIPLRTYLK HSTCPKYLKA 2170 2180 2190 2200 VLGITKLKEM FTDTSVLYLT RAQQKFYMKT IGNAVKGYYS NCDS

References

[1]	"Nucleotide sequence analysis of the L gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses." Yusoff K., Millar N.S., Chambers P., Emmerson P.T. Nucleic Acids Res. 15:3961-3976(1987) [PubMed: 3035486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05399 Genomic RNA. Translation: CAA28985.1.
PIR	RRNZNV. A26747.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR016269. RNA-dir_RNA_pol_paramyxovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_NDVB

Accession

Primary (citable) accession number: P11205

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)