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Reviewed, UniProtKB/Swiss-Prot P11204 (POL_EIAV9)

Last modified February 9, 2010. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pol polyprotein
Cleaved into the following 3 chains:
    1- Recommended name:
            Protease
        Alternative name(s):
            Retropepsin
            EC=3.4.23.-
    2- Recommended name:
            Reverse transcriptase/ribonuclease H
                Short name=RT
              EC=2.7.7.49
              EC=3.1.26.4
    3- Recommended name:
            Integrase
                Short name=IN
Gene names
Name: pol
OrganismEquine infectious anemia virus (isolate 1369) (EIAV)
Taxonomic identifier11670 [NCBI]
Taxonomic lineageViruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Equine lentivirus group
Virus hostEquus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

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Protein attributes

Sequence length1146 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins.

Sequence similarities

Belongs to the retroviral Pol polyprotein family.

Contains 1 integrase catalytic domain.

Contains 1 integrase-type DNA-binding domain.

Contains 1 integrase-type zinc finger.

Contains 1 peptidase A2 domain.

Contains 1 reverse transcriptase domain.

Contains 1 RNase H domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Protease
PRO_0000038838
Chain196 – 914719Reverse transcriptase/ribonuclease H
PRO_0000038839
Chain915 – 1146232Integrase
PRO_0000038840

Regions

Domain100 – 17475Peptidase A2
Domain230 – 419190Reverse transcriptase
Domain617 – 740124RNase H
Domain921 – 1078158Integrase catalytic
Zinc finger878 – 91942Integrase-type
DNA binding1096 – 114449Integrase-type

Sites

Active site1051 By similarity

Secondary structure

1 ........................... 1146
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P11204-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 2BEB2E04F93FA36F

FASTA1,146129,510
        10         20         30         40         50         60 
TAWTFLKAMQ KCSKKREARG SREAPETNFP DTTEESAQQI CCTRDSSDSK SVPRSERNKK 

        70         80         90        100        110        120 
GIQCQGEGSS RGSQPGQFVG VTYNLEKRPT TIVLINDTPL NVLLDTGADT SVLTTAHYNR 

       130        140        150        160        170        180 
LKYRGRKYQG TGIIGVGGNV ETFSTPVTIK KKGRHIKTRM LVADIPVTIL GRDILQDLGA 

       190        200        210        220        230        240 
KLVLAQLSKE IKFRKIELKE GTMGPKIPQW PLTKEKLEGA KEIVQRLLSE GKISEASDNN 

       250        260        270        280        290        300 
PYNSPIFVIK KRSGKWRLLQ DLRELNKTVQ VGTEISRGLP HPGGLIKCKH MTVLDIGDAY 

       310        320        330        340        350        360 
FTIPLDPEFR PYTAFTIPSI NHQEPDKRYV WNCLPQGFVL SPYIYQKTLQ EILQPFRERY 

       370        380        390        400        410        420 
PEVQLYQYMD DLFVGSNGSK KQHKELIIEL RAILLEEGFE TPDDKLQEVP PYSWLGYQLC 

       430        440        450        460        470        480 
PENWKVQKMQ LDMVKNPTLN DVQKLMGNIT WMSSGVPGLT VKHIAATTKG CLELNQKVIW 

       490        500        510        520        530        540 
TEEAQKELEE NNEKIKNAQG LQYYNPEEEM LCEVEITKNY EATYVIKQSQ GILWAGKKIM 

       550        560        570        580        590        600 
KANKGWSTVK NLMLLLQHVA TESITRVGKC PTFKVPFTKE QVMWEMQKGW YYSWLPEIVY 

       610        620        630        640        650        660 
THQVVHDDWR MKLVEEPTSG ITIYTDGGKQ NGEGIAAYVT SNGRTKQKRL GPVTHQVAER 

       670        680        690        700        710        720 
MAIQMALEDT RDKQVNIVTD SYYCWKNITE GLGLEGPQSP WWPIIQNIRE KEIVYFAWVP 

       730        740        750        760        770        780 
GHKGICGNQL ADEAAKIKEE IMLAYQGTQI KEKRDEDAGF DLCVPYDIMI PVSDTKIIPT 

       790        800        810        820        830        840 
DVKIQVPPNS FGWVTGKSSM AKQGLLINGG IIDEGYTGEI QVICTNIGKS NIKLIEGQKF 

       850        860        870        880        890        900 
AQLIILQHHS NSRQPWDENK ISQRGDKGFG STGVFWVENI QEAQDEHENW HTSPKILARN 

       910        920        930        940        950        960 
YKIPLTVAKQ ITQECPHCTK QGSGPAGCVM RSPNHWQADC THLDNKIILT FVESNSGYIH 

       970        980        990       1000       1010       1020 
ATLLSKENAL CTSLAILEWA RLFSPKSLHT DNGTNFVAEP VVNLLKFLKI AHTTGIPYHP 

      1030       1040       1050       1060       1070       1080 
ESQGIVERAN RTLKEKIQSH RDNTQTLEAA LQLALITCNK GRESMGGQTP WEVFITNQAQ 

      1090       1100       1110       1120       1130       1140 
VIHEKLLLQQ AQSSKKFCFY KIPGEHDWKG PTRVLWKGDG AVVVNDEGKG IIAVPLTRTK 


LLIKPN

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References

[1]"Nucleotide sequence analysis of equine infectious anemia virus proviral DNA."
Kawakami T., Sherman L., Dahlberg J., Gazit A., Yaniv A., Tronick S.R., Aaronson S.A.
Virology 158:300-312(1987) [PubMed: 3035786] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M16575 Genomic RNA. Translation: AAB59862.1. Different initiation.
PIRGNLJEW. B27842.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DUCX-ray2.05A742-875[»]
1DUNX-ray1.90A742-875[»]
SMRP11204. Positions 81-184, 194-603, 876-920, 932-1076, 1095-1141.
ModBaseSearch...

Family and domain databases

InterProIPR008180. DeoxyUTP_pyroPase_dom.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
IPR012337. PolynucTfrase_RNaseH_fold.
IPR000477. Reverse_transcriptase.
IPR002156. RNase_H.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]
Gene3DG3DSA:2.30.30.10. Integrase_C. 1 hit.
G3DSA:1.10.10.200. Intgrase_N_Zn_bd. 1 hit.
G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PfamPF00692. dUTPase. 1 hit.
PF00552. Integrase. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RnaseH. 1 hit.
PF00665. rve. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
PROSITEPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePOL_EIAV9
AccessionPrimary (citable) accession number: P11204
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 9, 2010
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents