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Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5UniRule annotation
Short name:
NSP5UniRule annotation
Alternative name(s):
NS26UniRule annotation
OrganismiRotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A) (Simian Agent 11 (strain Both))
Taxonomic identifieri37137 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesi
  • UP000007180 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496411 – 198Non-structural protein 5Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphoserine; by host CK1UniRule annotation1
Modified residuei154Phosphoserine; by hostUniRule annotation1
Modified residuei156Phosphoserine; by hostUniRule annotation1
Modified residuei164Phosphoserine; by hostUniRule annotation1
Modified residuei166Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

O-glycosylated.UniRule annotation
Hyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer.UniRule annotation

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6.UniRule annotation

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 101Ser-richAdd BLAST100

Sequence similaritiesi

Belongs to the rotavirus NSP5 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG09000168

Family and domain databases

HAMAPiMF_04092 ROTA_NSP5, 1 hit
InterProiView protein in InterPro
IPR002512 Rotavirus_A/C_NSP5
PfamiView protein in Pfam
PF01525 Rota_NS26, 1 hit
PIRSFiPIRSF004006 Rota_NS26, 1 hit

Sequencei

Sequence statusi: Complete.

P11202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSIDVTSL PSIPSTIYKN ESSSTTSTLS GKSIGRSEQY ISPDAEAFNK
60 70 80 90 100
YMLSKSPEDI GPSDSASNDP LTSFSIRSNA VKTNADAGVS MDSSAQSRPS
110 120 130 140 150
SNVGCDQVDF SLNKGLKVKA NLDSSISIST DTKKEKSKQN HKSRKHYPRI
160 170 180 190
EAESDSDDYV LDDSDSDDGK CKNCKYKKKY FALRMRMKQV AMQLIEDL
Length:198
Mass (Da):21,722
Last modified:July 1, 1989 - v1
Checksum:i1DE1EC4A803E1D57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07831 Genomic RNA Translation: CAA30683.1
M28347 Genomic RNA Translation: AAA66881.1
PIRiS01246 MNXRSE

Similar proteinsi

Entry informationi

Entry nameiNSP5_ROTS1
AccessioniPrimary (citable) accession number: P11202
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health