P11187 (LYS_BPPH2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
Endolysin
Lysis protein
Morphogenesis protein 2
Muramidase

Gene names

Name:

15

Organism

Bacillus phage phi29 (Bacteriophage phi-29)

Taxonomic identifier

10756 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Picovirinae › Phi29likevirus

Virus host

Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Helps to release the mature phage particles from the cell wall by breaking down the peptidoglycan.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Domain	LysM repeats are thought to be involved in peptidoglycan binding.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family. Contains 2 LysM repeats.

Ontologies

Keywords
Developmental stage	Late protein
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Lysozyme		PRO_0000218103
Regions
	Repeat	163 – 207	45	LysM 1
	Repeat	214 – 258	45	LysM 2
Sites
	Active site	15	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11187 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 26AE0C1D927B42D8	FASTA	258	28,054
	10 20 30 40 50 60 MQISQAGINL IKSFEGLQLK AYKAVPTEKH YTIGYGHYGS DVSPRQVITA KQAEDMLRDD 70 80 90 100 110 120 VQAFVDGVNK ALKVSVTQNQ FDALVSFAYN VGLGAFRSSS LLEYLNEGRT ALAAAEFPKW 130 140 150 160 170 180 NKSGGKVYQG LINRRAQEQA LFNSGTPKNV SRGTSSTKTT PKYKVKSGDN LTKIAKKHNT 190 200 210 220 230 240 TVATLLKLNP SIKDPNMIRV GQTINVTGSG GKTHKVKSGD TLSKIAVDNK TTVSRLMSLN 250 PEITNPNHIK VGQTIRLS

References

[1]	"Nucleotide sequence of Bacillus phage phi 29 genes 14 and 15: homology of gene 15 with other phage lysozymes." Garvey K.J., Saedi M.S., Ito J. Nucleic Acids Res. 14:10001-10008(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the late region of Bacillus phage phi 29 completes the 19,285-bp sequence of phi 29 genome. Comparison with the homologous sequence of phage PZA." Vlcek C., Paces V. Gene 46:215-225(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete sequence of Bacillus phage phi 29 gene 16: a protein required for the genome encapsidation reaction." Garvey K.J., Saedi M.S., Ito J. Gene 40:311-316(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-258.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04962 Genomic DNA. Translation: CAA28632.1. M14782 Genomic DNA. Translation: AAA32288.1. M14431 Genomic DNA. Translation: AAA88347.1.
PIR	WMBPP9. B24721.
RefSeq	YP_002004544.1. NC_011048.1.
3D structure databases
ProteinModelPortal	P11187.
ModBase	Search...
Protein family/group databases
CAZy	CBM50. Carbohydrate-Binding Module Family 50. GH24. Glycoside Hydrolase Family 24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6446520.
Phylogenomic databases
ProtClustDB	CLSP2343229.
Family and domain databases
Gene3D	1.10.530.40. 1 hit.
InterPro	IPR002196. Glyco_hydro_24. IPR023346. Lysozyme-like_dom. IPR023347. Lysozyme_dom. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF01476. LysM. 2 hits. PF00959. Phage_lysozyme. 1 hit. [Graphical view]
SMART	SM00257. LysM. 2 hits. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYS_BPPH2

Accession

Primary (citable) accession number: P11187

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program