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Protein

Endolysin

Gene

15

Organism
Bacillus phage phi29 (Bacteriophage phi-29)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.Curated1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei15 – 151Proton donorBy similarity

GO - Molecular functioni

  • lysozyme activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
GH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Gene product 15
Short name:
gp15
Lysozyme
Morphogenesis protein 2
Muramidase
Protein p15
Gene namesi
Name:15
OrganismiBacillus phage phi29 (Bacteriophage phi-29)
Taxonomic identifieri10756 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaePicovirinaePhi29likevirus
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000001207 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258EndolysinPRO_0000218103Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP11187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 20646LysM 1PROSITE-ProRule annotationAdd
BLAST
Domaini212 – 25746LysM 2PROSITE-ProRule annotationAdd
BLAST

Domaini

LysM domains are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated
Contains 2 LysM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK01185.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
3.10.350.10. 2 hits.
InterProiIPR002196. Glyco_hydro_24.
IPR018392. LysM_dom.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF01476. LysM. 2 hits.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF54106. SSF54106. 2 hits.
PROSITEiPS51782. LYSM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISQAGINL IKSFEGLQLK AYKAVPTEKH YTIGYGHYGS DVSPRQVITA
60 70 80 90 100
KQAEDMLRDD VQAFVDGVNK ALKVSVTQNQ FDALVSFAYN VGLGAFRSSS
110 120 130 140 150
LLEYLNEGRT ALAAAEFPKW NKSGGKVYQG LINRRAQEQA LFNSGTPKNV
160 170 180 190 200
SRGTSSTKTT PKYKVKSGDN LTKIAKKHNT TVATLLKLNP SIKDPNMIRV
210 220 230 240 250
GQTINVTGSG GKTHKVKSGD TLSKIAVDNK TTVSRLMSLN PEITNPNHIK

VGQTIRLS
Length:258
Mass (Da):28,054
Last modified:July 1, 1989 - v1
Checksum:i26AE0C1D927B42D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04962 Genomic DNA. Translation: CAA28632.1.
M14782 Genomic DNA. Translation: AAA32288.1.
EU771092 Genomic DNA. Translation: ACE96038.1.
M14431 Genomic DNA. Translation: AAA88347.1.
PIRiB24721. WMBPP9.
RefSeqiYP_002004544.1. NC_011048.1.

Genome annotation databases

GeneIDi6446520.
KEGGivg:6446520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04962 Genomic DNA. Translation: CAA28632.1.
M14782 Genomic DNA. Translation: AAA32288.1.
EU771092 Genomic DNA. Translation: ACE96038.1.
M14431 Genomic DNA. Translation: AAA88347.1.
PIRiB24721. WMBPP9.
RefSeqiYP_002004544.1. NC_011048.1.

3D structure databases

ProteinModelPortaliP11187.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
GH24. Glycoside Hydrolase Family 24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6446520.
KEGGivg:6446520.

Phylogenomic databases

KOiK01185.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
3.10.350.10. 2 hits.
InterProiIPR002196. Glyco_hydro_24.
IPR018392. LysM_dom.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF01476. LysM. 2 hits.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF54106. SSF54106. 2 hits.
PROSITEiPS51782. LYSM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of Bacillus phage phi 29 genes 14 and 15: homology of gene 15 with other phage lysozymes."
    Garvey K.J., Saedi M.S., Ito J.
    Nucleic Acids Res. 14:10001-10008(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the late region of Bacillus phage phi 29 completes the 19,285-bp sequence of phi 29 genome. Comparison with the homologous sequence of phage PZA."
    Vlcek C., Paces V.
    Gene 46:215-225(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Villegas A.P., Lingohr E.J., Ceyssens P.-J., Kropinski A.M.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The complete sequence of Bacillus phage phi 29 gene 16: a protein required for the genome encapsidation reaction."
    Garvey K.J., Saedi M.S., Ito J.
    Gene 40:311-316(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-258.
  5. "Cloning and purification of a unique lysozyme produced by Bacillus phage phi 29."
    Saedi M.S., Garvey K.J., Ito J.
    Proc. Natl. Acad. Sci. U.S.A. 84:955-958(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION, FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiENLYS_BPPH2
AccessioniPrimary (citable) accession number: P11187
Secondary accession number(s): B3VMQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.