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Protein

Endolysin

Gene

15

Organism
Bacillus phage phi29 (Bacteriophage phi-29)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.Curated1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Proton donorBy similarity1

GO - Molecular functioni

  • lysozyme activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase
Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Protein family/group databases

CAZyiCBM50 Carbohydrate-Binding Module Family 50
GH24 Glycoside Hydrolase Family 24

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Gene product 15
Short name:
gp15
Lysozyme
Morphogenesis protein 2
Muramidase
Protein p15
Gene namesi
Name:15
OrganismiBacillus phage phi29 (Bacteriophage phi-29)
Taxonomic identifieri10756 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaePicovirinaePhi29virus
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000001207 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated
  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181031 – 258EndolysinAdd BLAST258

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP11187
SMRiP11187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini161 – 206LysM 1PROSITE-ProRule annotationAdd BLAST46
Domaini212 – 257LysM 2PROSITE-ProRule annotationAdd BLAST46

Domaini

LysM domains are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

OrthoDBiVOG090000CE

Family and domain databases

CDDicd00737 endolysin_autolysin, 1 hit
cd00118 LysM, 2 hits
Gene3Di3.10.350.102 hits
InterProiView protein in InterPro
IPR033907 Endolysin_autolysin
IPR002196 Glyco_hydro_24
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF01476 LysM, 2 hits
PF00959 Phage_lysozyme, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 2 hits
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS51782 LYSM, 2 hits

Sequencei

Sequence statusi: Complete.

P11187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISQAGINL IKSFEGLQLK AYKAVPTEKH YTIGYGHYGS DVSPRQVITA
60 70 80 90 100
KQAEDMLRDD VQAFVDGVNK ALKVSVTQNQ FDALVSFAYN VGLGAFRSSS
110 120 130 140 150
LLEYLNEGRT ALAAAEFPKW NKSGGKVYQG LINRRAQEQA LFNSGTPKNV
160 170 180 190 200
SRGTSSTKTT PKYKVKSGDN LTKIAKKHNT TVATLLKLNP SIKDPNMIRV
210 220 230 240 250
GQTINVTGSG GKTHKVKSGD TLSKIAVDNK TTVSRLMSLN PEITNPNHIK

VGQTIRLS
Length:258
Mass (Da):28,054
Last modified:July 1, 1989 - v1
Checksum:i26AE0C1D927B42D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04962 Genomic DNA Translation: CAA28632.1
M14782 Genomic DNA Translation: AAA32288.1
EU771092 Genomic DNA Translation: ACE96038.1
M14431 Genomic DNA Translation: AAA88347.1
PIRiB24721 WMBPP9
RefSeqiYP_002004544.1, NC_011048.1

Genome annotation databases

GeneIDi6446520
KEGGivg:6446520

Similar proteinsi

Entry informationi

Entry nameiENLYS_BPPH2
AccessioniPrimary (citable) accession number: P11187
Secondary accession number(s): B3VMQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome