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Protein

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Gene

DBT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.1 Publication

Catalytic activityi

2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei452 – 4521Sequence analysis
Active sitei456 – 4561Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-BTA-389661. Glyoxylate metabolism and glycine degradation.
R-BTA-70895. Branched-chain amino acid catabolism.
SABIO-RKP11181.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (EC:2.3.1.168)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase complex component E2
Short name:
BCKAD-E2
Short name:
BCKADE2
Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
Dihydrolipoamide branched chain transacylase
Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
Gene namesi
Name:DBT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6161MitochondrionAdd
BLAST
Chaini62 – 482421Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrialPRO_0000020488Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051N6-lipoyllysinePROSITE-ProRule annotation1 Publication
Modified residuei133 – 1331N6-succinyllysineBy similarity
Modified residuei196 – 1961N6-acetyllysine; alternateBy similarity
Modified residuei196 – 1961N6-succinyllysine; alternateBy similarity
Modified residuei202 – 2021N6-acetyllysineBy similarity
Modified residuei220 – 2201PhosphoserineBy similarity
Modified residuei243 – 2431N6-acetyllysineBy similarity
Modified residuei250 – 2501N6-acetyllysineBy similarity
Modified residuei261 – 2611N6-succinyllysineBy similarity
Modified residuei289 – 2891N6-acetyllysine; alternateBy similarity
Modified residuei289 – 2891N6-succinyllysine; alternateBy similarity
Modified residuei295 – 2951N6-acetyllysineBy similarity
Modified residuei304 – 3041N6-acetyllysineBy similarity
Modified residuei435 – 4351N6-acetyllysineBy similarity
Modified residuei440 – 4401N6-acetyllysine; alternateBy similarity
Modified residuei440 – 4401N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11181.
PeptideAtlasiP11181.
PRIDEiP11181.

Expressioni

Tissue specificityi

Expressed in kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSBTAG00000006320.

Interactioni

Subunit structurei

Forms a 24-polypeptide structural core with octahedral symmetry.

Protein-protein interaction databases

IntActiP11181. 1 interaction.
STRINGi9913.ENSBTAP00000008292.

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi252 – 2554Combined sources
Helixi258 – 2603Combined sources
Helixi261 – 2699Combined sources
Helixi270 – 2723Combined sources
Beta strandi275 – 2839Combined sources
Helixi285 – 30016Combined sources
Helixi308 – 32114Combined sources
Helixi323 – 3253Combined sources
Beta strandi326 – 3294Combined sources
Beta strandi335 – 3384Combined sources
Beta strandi344 – 3463Combined sources
Beta strandi348 – 3503Combined sources
Beta strandi353 – 3553Combined sources
Helixi362 – 3643Combined sources
Helixi367 – 38317Combined sources
Helixi388 – 3914Combined sources
Beta strandi396 – 3994Combined sources
Helixi401 – 4033Combined sources
Beta strandi419 – 4235Combined sources
Beta strandi427 – 4326Combined sources
Beta strandi438 – 45114Combined sources
Turni452 – 4543Combined sources
Helixi457 – 47216Combined sources
Helixi475 – 4806Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IHWX-ray2.70A/B/C/D/E/F/G/H223-482[»]
2II3X-ray2.17A/B/C/D/E/F/G/H223-482[»]
2II4X-ray2.59A/B/C/D/E/F/G/H223-482[»]
2II5X-ray2.50A/B/C/D/E/F/G/H223-482[»]
ProteinModelPortaliP11181.
SMRiP11181. Positions 62-147, 163-220, 249-482.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11181.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 13976Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0558. Eukaryota.
COG0508. LUCA.
GeneTreeiENSGT00810000125418.
HOGENOMiHOG000281564.
HOVERGENiHBG104085.
InParanoidiP11181.
KOiK09699.
OMAiTIPHFTY.
OrthoDBiEOG091G0FJ3.
TreeFamiTF314182.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR015761. BCKDC_E2.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR23151:SF71. PTHR23151:SF71. 2 hits.
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11181-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAALVLRTW SRAAGQLICV RYFQTCGNVH VLKPKYVCFF GYPPFKYSHP
60 70 80 90 100
YQWLKTTAAL QGQIVQFKLS DIGEGIREVT VKEWYVKEGD TVSQFDSICE
110 120 130 140 150
VQSDKASVTI TSRYDGVIKK LYYNLDDTAY VGKPLVDIET EALKDSEEDV
160 170 180 190 200
VETPAVSHDE HTHQEIKGQK TLATPAVRRL AMENNIKLSE VIGSGKDGRI
210 220 230 240 250
LKEDILNYLE KQTGAILPPS PKAEIMPPPP KPKDRTIPIP ISKPPVFIGK
260 270 280 290 300
DRTEPVKGFH KAMVKTMSAA LKIPHFGYCD EVDLTELVKL REELKPIAFA
310 320 330 340 350
RGIKLSFMPF FLKAASLGLL QFPILNASVD ENCQNITYKA SHNIGIAMDT
360 370 380 390 400
EQGLIVPNVK NVQIRSIFEI ATELNRLQKL GSAGQLSTND LIGGTFTLSN
410 420 430 440 450
IGSIGGTYAK PVILPPEVAI GALGTIKALP RFNEKGEVCK AQIMNVSWSA
460 470 480
DHRIIDGATV SRFSNLWKSY LENPAFMLLD LK
Length:482
Mass (Da):53,410
Last modified:February 1, 1996 - v2
Checksum:i70F06EE62B14B5C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731A → G in AAA30596 (PubMed:2837277).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21572 mRNA. Translation: AAA30597.1.
BC134527 mRNA. Translation: AAI34528.1.
M19475 mRNA. Translation: AAA30596.1.
PIRiA30801. XUBOLA.
RefSeqiNP_776330.1. NM_173905.2.
UniGeneiBt.107201.

Genome annotation databases

EnsembliENSBTAT00000008292; ENSBTAP00000008292; ENSBTAG00000006320.
GeneIDi280759.
KEGGibta:280759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21572 mRNA. Translation: AAA30597.1.
BC134527 mRNA. Translation: AAI34528.1.
M19475 mRNA. Translation: AAA30596.1.
PIRiA30801. XUBOLA.
RefSeqiNP_776330.1. NM_173905.2.
UniGeneiBt.107201.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IHWX-ray2.70A/B/C/D/E/F/G/H223-482[»]
2II3X-ray2.17A/B/C/D/E/F/G/H223-482[»]
2II4X-ray2.59A/B/C/D/E/F/G/H223-482[»]
2II5X-ray2.50A/B/C/D/E/F/G/H223-482[»]
ProteinModelPortaliP11181.
SMRiP11181. Positions 62-147, 163-220, 249-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11181. 1 interaction.
STRINGi9913.ENSBTAP00000008292.

Proteomic databases

PaxDbiP11181.
PeptideAtlasiP11181.
PRIDEiP11181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008292; ENSBTAP00000008292; ENSBTAG00000006320.
GeneIDi280759.
KEGGibta:280759.

Organism-specific databases

CTDi1629.

Phylogenomic databases

eggNOGiKOG0558. Eukaryota.
COG0508. LUCA.
GeneTreeiENSGT00810000125418.
HOGENOMiHOG000281564.
HOVERGENiHBG104085.
InParanoidiP11181.
KOiK09699.
OMAiTIPHFTY.
OrthoDBiEOG091G0FJ3.
TreeFamiTF314182.

Enzyme and pathway databases

ReactomeiR-BTA-389661. Glyoxylate metabolism and glycine degradation.
R-BTA-70895. Branched-chain amino acid catabolism.
SABIO-RKP11181.

Miscellaneous databases

EvolutionaryTraceiP11181.

Gene expression databases

BgeeiENSBTAG00000006320.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR015761. BCKDC_E2.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR23151:SF71. PTHR23151:SF71. 2 hits.
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiODB2_BOVIN
AccessioniPrimary (citable) accession number: P11181
Secondary accession number(s): A7YWE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.