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Protein

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Gene

DBT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.1 Publication

Catalytic activityi

2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei452Sequence analysis1
Active sitei456Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-BTA-389661. Glyoxylate metabolism and glycine degradation.
R-BTA-70895. Branched-chain amino acid catabolism.
SABIO-RKP11181.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (EC:2.3.1.168)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase complex component E2
Short name:
BCKAD-E2
Short name:
BCKADE2
Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
Dihydrolipoamide branched chain transacylase
Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
Gene namesi
Name:DBT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 61MitochondrionAdd BLAST61
ChainiPRO_000002048862 – 482Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrialAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei105N6-lipoyllysinePROSITE-ProRule annotation1 Publication1
Modified residuei133N6-succinyllysineBy similarity1
Modified residuei196N6-acetyllysine; alternateBy similarity1
Modified residuei196N6-succinyllysine; alternateBy similarity1
Modified residuei202N6-acetyllysineBy similarity1
Modified residuei220PhosphoserineBy similarity1
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei250N6-acetyllysineBy similarity1
Modified residuei261N6-succinyllysineBy similarity1
Modified residuei289N6-acetyllysine; alternateBy similarity1
Modified residuei289N6-succinyllysine; alternateBy similarity1
Modified residuei295N6-acetyllysineBy similarity1
Modified residuei304N6-acetyllysineBy similarity1
Modified residuei435N6-acetyllysineBy similarity1
Modified residuei440N6-acetyllysine; alternateBy similarity1
Modified residuei440N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11181.
PeptideAtlasiP11181.
PRIDEiP11181.

Expressioni

Tissue specificityi

Expressed in kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSBTAG00000006320.

Interactioni

Subunit structurei

Forms a 24-polypeptide structural core with octahedral symmetry.

Protein-protein interaction databases

IntActiP11181. 1 interactor.
STRINGi9913.ENSBTAP00000008292.

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi252 – 255Combined sources4
Helixi258 – 260Combined sources3
Helixi261 – 269Combined sources9
Helixi270 – 272Combined sources3
Beta strandi275 – 283Combined sources9
Helixi285 – 300Combined sources16
Helixi308 – 321Combined sources14
Helixi323 – 325Combined sources3
Beta strandi326 – 329Combined sources4
Beta strandi335 – 338Combined sources4
Beta strandi344 – 346Combined sources3
Beta strandi348 – 350Combined sources3
Beta strandi353 – 355Combined sources3
Helixi362 – 364Combined sources3
Helixi367 – 383Combined sources17
Helixi388 – 391Combined sources4
Beta strandi396 – 399Combined sources4
Helixi401 – 403Combined sources3
Beta strandi419 – 423Combined sources5
Beta strandi427 – 432Combined sources6
Beta strandi438 – 451Combined sources14
Turni452 – 454Combined sources3
Helixi457 – 472Combined sources16
Helixi475 – 480Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHWX-ray2.70A/B/C/D/E/F/G/H223-482[»]
2II3X-ray2.17A/B/C/D/E/F/G/H223-482[»]
2II4X-ray2.59A/B/C/D/E/F/G/H223-482[»]
2II5X-ray2.50A/B/C/D/E/F/G/H223-482[»]
ProteinModelPortaliP11181.
SMRiP11181.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11181.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 139Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0558. Eukaryota.
COG0508. LUCA.
GeneTreeiENSGT00860000133743.
HOGENOMiHOG000281564.
HOVERGENiHBG104085.
InParanoidiP11181.
KOiK09699.
OMAiTIPHFTY.
OrthoDBiEOG091G0FJ3.
TreeFamiTF314182.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR015761. BCKDC_E2.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR23151:SF71. PTHR23151:SF71. 2 hits.
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11181-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAALVLRTW SRAAGQLICV RYFQTCGNVH VLKPKYVCFF GYPPFKYSHP
60 70 80 90 100
YQWLKTTAAL QGQIVQFKLS DIGEGIREVT VKEWYVKEGD TVSQFDSICE
110 120 130 140 150
VQSDKASVTI TSRYDGVIKK LYYNLDDTAY VGKPLVDIET EALKDSEEDV
160 170 180 190 200
VETPAVSHDE HTHQEIKGQK TLATPAVRRL AMENNIKLSE VIGSGKDGRI
210 220 230 240 250
LKEDILNYLE KQTGAILPPS PKAEIMPPPP KPKDRTIPIP ISKPPVFIGK
260 270 280 290 300
DRTEPVKGFH KAMVKTMSAA LKIPHFGYCD EVDLTELVKL REELKPIAFA
310 320 330 340 350
RGIKLSFMPF FLKAASLGLL QFPILNASVD ENCQNITYKA SHNIGIAMDT
360 370 380 390 400
EQGLIVPNVK NVQIRSIFEI ATELNRLQKL GSAGQLSTND LIGGTFTLSN
410 420 430 440 450
IGSIGGTYAK PVILPPEVAI GALGTIKALP RFNEKGEVCK AQIMNVSWSA
460 470 480
DHRIIDGATV SRFSNLWKSY LENPAFMLLD LK
Length:482
Mass (Da):53,410
Last modified:February 1, 1996 - v2
Checksum:i70F06EE62B14B5C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173A → G in AAA30596 (PubMed:2837277).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21572 mRNA. Translation: AAA30597.1.
BC134527 mRNA. Translation: AAI34528.1.
M19475 mRNA. Translation: AAA30596.1.
PIRiA30801. XUBOLA.
RefSeqiNP_776330.1. NM_173905.2.
UniGeneiBt.107201.

Genome annotation databases

EnsembliENSBTAT00000008292; ENSBTAP00000008292; ENSBTAG00000006320.
GeneIDi280759.
KEGGibta:280759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21572 mRNA. Translation: AAA30597.1.
BC134527 mRNA. Translation: AAI34528.1.
M19475 mRNA. Translation: AAA30596.1.
PIRiA30801. XUBOLA.
RefSeqiNP_776330.1. NM_173905.2.
UniGeneiBt.107201.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHWX-ray2.70A/B/C/D/E/F/G/H223-482[»]
2II3X-ray2.17A/B/C/D/E/F/G/H223-482[»]
2II4X-ray2.59A/B/C/D/E/F/G/H223-482[»]
2II5X-ray2.50A/B/C/D/E/F/G/H223-482[»]
ProteinModelPortaliP11181.
SMRiP11181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11181. 1 interactor.
STRINGi9913.ENSBTAP00000008292.

Proteomic databases

PaxDbiP11181.
PeptideAtlasiP11181.
PRIDEiP11181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008292; ENSBTAP00000008292; ENSBTAG00000006320.
GeneIDi280759.
KEGGibta:280759.

Organism-specific databases

CTDi1629.

Phylogenomic databases

eggNOGiKOG0558. Eukaryota.
COG0508. LUCA.
GeneTreeiENSGT00860000133743.
HOGENOMiHOG000281564.
HOVERGENiHBG104085.
InParanoidiP11181.
KOiK09699.
OMAiTIPHFTY.
OrthoDBiEOG091G0FJ3.
TreeFamiTF314182.

Enzyme and pathway databases

ReactomeiR-BTA-389661. Glyoxylate metabolism and glycine degradation.
R-BTA-70895. Branched-chain amino acid catabolism.
SABIO-RKP11181.

Miscellaneous databases

EvolutionaryTraceiP11181.

Gene expression databases

BgeeiENSBTAG00000006320.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR015761. BCKDC_E2.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR23151:SF71. PTHR23151:SF71. 2 hits.
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiODB2_BOVIN
AccessioniPrimary (citable) accession number: P11181
Secondary accession number(s): A7YWE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.