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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathway: lactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donorUniRule annotation
Active sitei375 – 3751NucleophileUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2746.
SABIO-RKP11175.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4704706-phospho-beta-galactosidasePRO_0000063890Add
BLAST

Proteomic databases

PRIDEiP11175.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV2189.

Structurei

3D structure databases

ProteinModelPortaliP11175.
SMRiP11175. Positions 1-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTLPEDFI FGGATAAYQA EGATNTDGKG RVAWDTYLEE NYWYTAEPAS
60 70 80 90 100
DFYNRYPVDL ELSEKFGVNG IRISIAWSRI FPNGYGEVNP KGVEYYHKLF
110 120 130 140 150
AECHKRHVEP FVTLHHFDTP EVLHKDGDFL NRKTIDYFVD YAEYCFKEFP
160 170 180 190 200
EVKYWTTFNE IGPIGDGQYL VGKFPPGIKY DFEKVFQSHH NMMVAHARAV
210 220 230 240 250
KLFKDGGYKG EIGVVHALPT KYPFDPSNPE DVRAAELEDI IHNKFILDAT
260 270 280 290 300
YLGKYSRETM EGVQHILSVN GGKLNITDED YAILDAAKDL NDFLGINYYM
310 320 330 340 350
SDWMRGYDGE SEITHNATGD KGGSKYQLKG VGQREFDVDV PRTDWDWMIY
360 370 380 390 400
PQGLYDQIMR VVKDYPNYHK IYITENGLGY KDEFIESEKT VHDDARIDYV
410 420 430 440 450
RQHLNVIADA IIDGANVKGY FIWSLMDVFS WSNGYEKRYG LFYVDFETQE
460 470
RYPKKSAYWY KELAETKEIK
Length:470
Mass (Da):54,550
Last modified:July 1, 1989 - v1
Checksum:i9A50A44B5ABFE74F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03479 Genomic DNA. Translation: AAA26650.1.
PIRiA27233.
RefSeqiWP_000169220.1. NZ_KN050644.1.

Genome annotation databases

GeneIDi23198030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03479 Genomic DNA. Translation: AAA26650.1.
PIRiA27233.
RefSeqiWP_000169220.1. NZ_KN050644.1.

3D structure databases

ProteinModelPortaliP11175.
SMRiP11175. Positions 1-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV2189.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEiP11175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23198030.

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
BioCyciMetaCyc:MONOMER-2746.
SABIO-RKP11175.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide and deduced amino acid sequences of the Staphylococcus aureus phospho-beta-galactosidase gene."
    Breidt F. Jr., Stewart G.C.
    Appl. Environ. Microbiol. 53:969-973(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors."
    Staedtler P., Hoenig S., Frank R., Withers S.G., Hengstenberg W.
    Eur. J. Biochem. 232:658-663(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE GLU-375.

Entry informationi

Entry nameiLACG_STAAU
AccessioniPrimary (citable) accession number: P11175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 24, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.