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P11158

- RIR2_VACCW

UniProt

P11158 - RIR2_VACCW

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Protein
Ribonucleoside-diphosphate reductase small chain
Gene
VACWR043, F4L
Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.1 Publication

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1 By similarity
Metal bindingi101 – 1011Iron 1 By similarity
Metal bindingi101 – 1011Iron 2 By similarity
Metal bindingi104 – 1041Iron 1 By similarity
Active sitei108 – 1081 By similarity
Metal bindingi163 – 1631Iron 2 By similarity
Metal bindingi197 – 1971Iron 2 By similarity
Metal bindingi200 – 2001Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Ordered Locus Names:VACWR043
ORF Names:F4L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Ribonucleoside-diphosphate reductase small chain
PRO_0000190498Add
BLAST

Expressioni

Inductioni

Expressed early in the viral replicative cycle.2 Publications

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Protein-protein interaction databases

DIPiDIP-2157N.
IntActiP11158. 1 interaction.
MINTiMINT-130990.

Structurei

3D structure databases

ProteinModelPortaliP11158.
SMRiP11158. Positions 2-282.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11158-1 [UniParc]FASTAAdd to Basket

« Hide

MEPILAPNPN RFVIFPIQYY DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
LTPDEKYFIK HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFQMAI 100
ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
CDFACLMFKH LLHPPSEETV RSIITDAVSI EQEFLTAALP VKLIGMNCEM 250
MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY 300
QKMGVMSQED NHFSLDVDF 319
Length:319
Mass (Da):36,973
Last modified:July 1, 1989 - v1
Checksum:i8E296CCC5AB4F949
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34368 mRNA. Translation: AAA48244.1.
M19117 Genomic DNA. Translation: AAA88680.1.
AY243312 Genomic DNA. Translation: AAO89322.1.
PIRiA29892. RDVZVV.
RefSeqiYP_232925.1. NC_006998.1.

Genome annotation databases

GeneIDi3707500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34368 mRNA. Translation: AAA48244.1 .
M19117 Genomic DNA. Translation: AAA88680.1 .
AY243312 Genomic DNA. Translation: AAO89322.1 .
PIRi A29892. RDVZVV.
RefSeqi YP_232925.1. NC_006998.1.

3D structure databases

ProteinModelPortali P11158.
SMRi P11158. Positions 2-282.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-2157N.
IntActi P11158. 1 interaction.
MINTi MINT-130990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707500.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb."
    Roseman N.A., Slabaugh M.B.
    Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  2. "Vaccinia virus-encoded ribonucleotide reductase: sequence conservation of the gene for the small subunit and its amplification in hydroxyurea-resistant mutants."
    Slabaugh M., Roseman N., Davis R., Mathews C.
    J. Virol. 62:519-527(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Hydroxyurea-resistant vaccinia virus: overproduction of ribonucleotide reductase."
    Slabaugh M.B., Mathews C.K.
    J. Virol. 60:506-514(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, INDUCTION.

Entry informationi

Entry nameiRIR2_VACCW
AccessioniPrimary (citable) accession number: P11158
Secondary accession number(s): Q76ZX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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