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P11158

- RIR2_VACCW

UniProt

P11158 - RIR2_VACCW

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

VACWR043

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.1 PublicationPROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 2By similarity
    Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
    Active sitei108 – 1081PROSITE-ProRule annotation
    Metal bindingi163 – 1631Iron 2By similarity
    Metal bindingi197 – 1971Iron 2By similarity
    Metal bindingi200 – 2001Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Ordered Locus Names:VACWR043
    ORF Names:F4L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190498Add
    BLAST

    Expressioni

    Inductioni

    Expressed early in the viral replicative cycle.2 Publications

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-2157N.
    IntActiP11158. 1 interaction.
    MINTiMINT-130990.

    Structurei

    3D structure databases

    ProteinModelPortaliP11158.
    SMRiP11158. Positions 2-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPILAPNPN RFVIFPIQYY DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
    LTPDEKYFIK HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFQMAI 100
    ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
    GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
    CDFACLMFKH LLHPPSEETV RSIITDAVSI EQEFLTAALP VKLIGMNCEM 250
    MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY 300
    QKMGVMSQED NHFSLDVDF 319
    Length:319
    Mass (Da):36,973
    Last modified:July 1, 1989 - v1
    Checksum:i8E296CCC5AB4F949
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34368 mRNA. Translation: AAA48244.1.
    M19117 Genomic DNA. Translation: AAA88680.1.
    AY243312 Genomic DNA. Translation: AAO89322.1.
    PIRiA29892. RDVZVV.
    RefSeqiYP_232925.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34368 mRNA. Translation: AAA48244.1 .
    M19117 Genomic DNA. Translation: AAA88680.1 .
    AY243312 Genomic DNA. Translation: AAO89322.1 .
    PIRi A29892. RDVZVV.
    RefSeqi YP_232925.1. NC_006998.1.

    3D structure databases

    ProteinModelPortali P11158.
    SMRi P11158. Positions 2-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-2157N.
    IntActi P11158. 1 interaction.
    MINTi MINT-130990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3707500.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb."
      Roseman N.A., Slabaugh M.B.
      Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    2. "Vaccinia virus-encoded ribonucleotide reductase: sequence conservation of the gene for the small subunit and its amplification in hydroxyurea-resistant mutants."
      Slabaugh M., Roseman N., Davis R., Mathews C.
      J. Virol. 62:519-527(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Hydroxyurea-resistant vaccinia virus: overproduction of ribonucleotide reductase."
      Slabaugh M.B., Mathews C.K.
      J. Virol. 60:506-514(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY, INDUCTION.

    Entry informationi

    Entry nameiRIR2_VACCW
    AccessioniPrimary (citable) accession number: P11158
    Secondary accession number(s): Q76ZX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3