P11156 (RIR2_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase subunit beta
EC=1.17.4.1

Alternative name(s):
Protein B2
Ribonucleotide reductase

Gene names

Name:

NRDB

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Ribonucleoside-diphosphate reductase subunit beta		PRO_0000190490
Sites
	Active site	122	1	By similarity
	Metal binding	84	1	Iron 1 By similarity
	Metal binding	115	1	Iron 1 By similarity
	Metal binding	115	1	Iron 2 By similarity
	Metal binding	118	1	Iron 1 By similarity
	Metal binding	212	1	Iron 2 By similarity
	Metal binding	247	1	Iron 2 By similarity
	Metal binding	250	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11156 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: E08F021DEF7DFB30	FASTA	388	45,354
	10 20 30 40 50 60 MSTVFNTNPV DVLNEPMFFG SGLGLARYDI QRHRVFEELI ERQISFFWRP EEVNLMMDAA 70 80 90 100 110 120 QFNKLPQYQQ NIFTNNLKYQ SLLDSIQGRA PSAVLMSLIS DPSLDTWVAT WTFSETIHSR 130 140 150 160 170 180 SYTHIMRNLY TDPSKVFDEI VLDEAIMKRA ESIGRYYDDV LVKTREWENA KDMVEYYKDQ 190 200 210 220 230 240 GLILADKDVE QRAKRDLMKS LYLCLHVINA LEAIRFYVSF ACTFNFHKNM EIMEGNAKIM 250 260 270 280 290 300 KFIARDEQLH LKGTQYIIRQ LQSGTDGDEW VKIAQECEQE AVDIFMEVNR QEKDWAVHLF 310 320 330 340 350 360 KDGDVPGLNT NSMWSFIDYL TVSRMKQCGL PCPITDAPVK HPYPWIREYL NSDNVQSAPQ 370 380 EVELSSYLVA QIDNDVDDKV MMSFKKYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The bacteriophage T4 gene for the small subunit of ribonucleotide reductase contains an intron." Sjoeberg B.-M., Hahne S., Mathews C.Z., Mathews C.K., Rand K.N., Gait M.J. EMBO J. 5:2031-2036(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04140 Genomic DNA. Translation: CAA27757.1. AF158101 Genomic DNA. Translation: AAD42624.1.
PIR	RDBP24. S07190.
RefSeq	NP_049841.1. NC_000866.4.
3D structure databases
ProteinModelPortal	P11156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1258601.
Phylogenomic databases
ProtClustDB	CLSP2343451.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 2 hits. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_BPT4

Accession

Primary (citable) accession number: P11156
Secondary accession number(s): Q96211

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program