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Protein

Pyruvate carboxylase 1

Gene

PYC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Miscellaneous

Present with 12500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136ATPBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei255ATPBy similarity1
Active sitei312By similarity1
Metal bindingi566Divalent metal cationBy similarity1
Binding sitei638SubstrateBy similarity1
Metal bindingi734Divalent metal cation; via carbamate groupBy similarity1
Metal bindingi764Divalent metal cationBy similarity1
Metal bindingi766Divalent metal cationBy similarity1
Binding sitei898SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • biotin binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • pyruvate carboxylase activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: SGD
  • pyruvate metabolic process Source: InterPro

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGluconeogenesis
LigandATP-binding, Biotin, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGL062W-MONOMER
BRENDAi6.4.1.1 984
ReactomeiR-SCE-196780 Biotin transport and metabolism
R-SCE-70263 Gluconeogenesis
SABIO-RKiP11154
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase 1 (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase 1
Short name:
PCB 1
Gene namesi
Name:PYC1
Synonyms:PYV
Ordered Locus Names:YGL062W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL062W
SGDiS000003030 PYC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001468241 – 1178Pyruvate carboxylase 1Add BLAST1178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei734N6-carboxylysineBy similarity1
Modified residuei1135N6-biotinyllysinePROSITE-ProRule annotation1 Publication1

Proteomic databases

MaxQBiP11154
PaxDbiP11154
PRIDEiP11154

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-14358,EBI-16219

Protein-protein interaction databases

BioGridi33185, 120 interactors
DIPiDIP-6425N
IntActiP11154, 17 interactors
MINTiP11154
STRINGi4932.YGL062W

Structurei

3D structure databases

ProteinModelPortaliP11154
SMRiP11154
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 470Biotin carboxylationAdd BLAST453
Domaini140 – 337ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini557 – 824Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST268
Domaini1094 – 1169Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni565 – 569Substrate bindingBy similarity5

Phylogenomic databases

GeneTreeiENSGT00900000141164
HOGENOMiHOG000282801
InParanoidiP11154
KOiK01958
OMAiEFVDACN
OrthoDBiEOG092C0BFY

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00866 CPSASE_1, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

P11154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRKFAGLR DNFNLLGEKN KILVANRGEI PIRIFRTAHE LSMQTVAIYS
60 70 80 90 100
HEDRLSTHKQ KADEAYVIGE VGQYTPVGAY LAIDEIISIA QKHQVDFIHP
110 120 130 140 150
GYGFLSENSE FADKVVKAGI TWIGPPAEVI DSVGDKVSAR NLAAKANVPT
160 170 180 190 200
VPGTPGPIET VEEALDFVNE YGYPVIIKAA FGGGGRGMRV VREGDDVADA
210 220 230 240 250
FQRATSEART AFGNGTCFVE RFLDKPKHIE VQLLADNHGN VVHLFERDCS
260 270 280 290 300
VQRRHQKVVE VAPAKTLPRE VRDAILTDAV KLAKECGYRN AGTAEFLVDN
310 320 330 340 350
QNRHYFIEIN PRIQVEHTIT EEITGIDIVA AQIQIAAGAS LPQLGLFQDK
360 370 380 390 400
ITTRGFAIQC RITTEDPAKN FQPDTGRIEV YRSAGGNGVR LDGGNAYAGT
410 420 430 440 450
IISPHYDSML VKCSCSGSTY EIVRRKMIRA LIEFRIRGVK TNIPFLLTLL
460 470 480 490 500
TNPVFIEGTY WTTFIDDTPQ LFQMVSSQNR AQKLLHYLAD VAVNGSSIKG
510 520 530 540 550
QIGLPKLKSN PSVPHLHDAQ GNVINVTKSA PPSGWRQVLL EKGPAEFARQ
560 570 580 590 600
VRQFNGTLLM DTTWRDAHQS LLATRVRTHD LATIAPTTAH ALAGRFALEC
610 620 630 640 650
WGGATFDVAM RFLHEDPWER LRKLRSLVPN IPFQMLLRGA NGVAYSSLPD
660 670 680 690 700
NAIDHFVKQA KDNGVDIFRV FDALNDLEQL KVGVDAVKKA GGVVEATVCF
710 720 730 740 750
SGDMLQPGKK YNLDYYLEIA EKIVQMGTHI LGIKDMAGTM KPAAAKLLIG
760 770 780 790 800
SLRAKYPDLP IHVHTHDSAG TAVASMTACA LAGADVVDVA INSMSGLTSQ
810 820 830 840 850
PSINALLASL EGNIDTGINV EHVRELDAYW AEMRLLYSCF EADLKGPDPE
860 870 880 890 900
VYQHEIPGGQ LTNLLFQAQQ LGLGEQWAET KRAYREANYL LGDIVKVTPT
910 920 930 940 950
SKVVGDLAQF MVSNKLTSDD VRRLANSLDF PDSVMDFFEG LIGQPYGGFP
960 970 980 990 1000
EPFRSDVLRN KRRKLTCRPG LELEPFDLEK IREDLQNRFG DVDECDVASY
1010 1020 1030 1040 1050
NMYPRVYEDF QKMRETYGDL SVLPTRSFLS PLETDEEIEV VIEQGKTLII
1060 1070 1080 1090 1100
KLQAVGDLNK KTGEREVYFD LNGEMRKIRV ADRSQKVETV TKSKADMHDP
1110 1120 1130 1140 1150
LHIGAPMAGV IVEVKVHKGS LIKKGQPVAV LSAMKMEMII SSPSDGQVKE
1160 1170
VFVSDGENVD SSDLLVLLED QVPVETKA
Length:1,178
Mass (Da):130,099
Last modified:October 1, 1996 - v2
Checksum:iBC7110A8AFB23E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti462T → G in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti493V → D in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti595R → A in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti619E → Q in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti664G → S in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti772A → R in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti879E → Q in AAA34843 (PubMed:3042770).Curated1
Sequence conflicti909Q → K in AAA34843 (PubMed:3042770).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03889 Genomic DNA Translation: AAA34843.1
Z72584 Genomic DNA Translation: CAA96765.1
BK006941 Genomic DNA Translation: DAA08040.1
PIRiS64066 QYBYP
RefSeqiNP_011453.1, NM_001180927.1

Genome annotation databases

EnsemblFungiiYGL062W; YGL062W; YGL062W
GeneIDi852818
KEGGisce:YGL062W

Similar proteinsi

Entry informationi

Entry nameiPYC1_YEAST
AccessioniPrimary (citable) accession number: P11154
Secondary accession number(s): D6VU79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 186 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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