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P11154

- PYC1_YEAST

UniProt

P11154 - PYC1_YEAST

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Protein
Pyruvate carboxylase 1
Gene
PYC1, PYV, YGL062W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactori

Biotin.
Zinc.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361ATP By similarity
Binding sitei220 – 2201ATP By similarity
Binding sitei255 – 2551ATP By similarity
Active sitei312 – 3121 By similarity
Metal bindingi566 – 5661Divalent metal cation By similarity
Binding sitei638 – 6381Substrate By similarity
Metal bindingi734 – 7341Divalent metal cation; via carbamate group By similarity
Metal bindingi764 – 7641Divalent metal cation By similarity
Metal bindingi766 – 7661Divalent metal cation By similarity
Binding sitei898 – 8981Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. biotin carboxylase activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: IntAct
  6. pyruvate carboxylase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Biotin, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGL062W-MONOMER.
BRENDAi6.4.1.1. 984.
ReactomeiREACT_188404. Defective HLCS causes multiple carboxylase deficiency.
REACT_188774. Biotin transport and metabolism.
SABIO-RKiP11154.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase 1 (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase 1
Short name:
PCB 1
Gene namesi
Name:PYC1
Synonyms:PYV
Ordered Locus Names:YGL062W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL062w.
SGDiS000003030. PYC1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Pyruvate carboxylase 1
PRO_0000146824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei734 – 7341N6-carboxylysine By similarity
Modified residuei1135 – 11351N6-biotinyllysine

Proteomic databases

MaxQBiP11154.
PeptideAtlasiP11154.
PRIDEiP11154.

Expressioni

Gene expression databases

GenevestigatoriP11154.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-14358,EBI-16219

Protein-protein interaction databases

BioGridi33185. 31 interactions.
DIPiDIP-6425N.
IntActiP11154. 5 interactions.
MINTiMINT-700616.
STRINGi4932.YGL062W.

Structurei

3D structure databases

ProteinModelPortaliP11154.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 470453Biotin carboxylation
Add
BLAST
Domaini140 – 337198ATP-grasp
Add
BLAST
Domaini557 – 824268Carboxyltransferase
Add
BLAST
Domaini1101 – 116868Biotinyl-binding
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni565 – 5695Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-grasp domain.

Phylogenomic databases

GeneTreeiENSGT00550000074986.
HOGENOMiHOG000282801.
KOiK01958.
OMAiYAIQSRV.
OrthoDBiEOG7GQZ41.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR18866:SF10. PTHR18866:SF10. 1 hit.
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view]
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11154-1 [UniParc]FASTAAdd to Basket

« Hide

MSQRKFAGLR DNFNLLGEKN KILVANRGEI PIRIFRTAHE LSMQTVAIYS     50
HEDRLSTHKQ KADEAYVIGE VGQYTPVGAY LAIDEIISIA QKHQVDFIHP 100
GYGFLSENSE FADKVVKAGI TWIGPPAEVI DSVGDKVSAR NLAAKANVPT 150
VPGTPGPIET VEEALDFVNE YGYPVIIKAA FGGGGRGMRV VREGDDVADA 200
FQRATSEART AFGNGTCFVE RFLDKPKHIE VQLLADNHGN VVHLFERDCS 250
VQRRHQKVVE VAPAKTLPRE VRDAILTDAV KLAKECGYRN AGTAEFLVDN 300
QNRHYFIEIN PRIQVEHTIT EEITGIDIVA AQIQIAAGAS LPQLGLFQDK 350
ITTRGFAIQC RITTEDPAKN FQPDTGRIEV YRSAGGNGVR LDGGNAYAGT 400
IISPHYDSML VKCSCSGSTY EIVRRKMIRA LIEFRIRGVK TNIPFLLTLL 450
TNPVFIEGTY WTTFIDDTPQ LFQMVSSQNR AQKLLHYLAD VAVNGSSIKG 500
QIGLPKLKSN PSVPHLHDAQ GNVINVTKSA PPSGWRQVLL EKGPAEFARQ 550
VRQFNGTLLM DTTWRDAHQS LLATRVRTHD LATIAPTTAH ALAGRFALEC 600
WGGATFDVAM RFLHEDPWER LRKLRSLVPN IPFQMLLRGA NGVAYSSLPD 650
NAIDHFVKQA KDNGVDIFRV FDALNDLEQL KVGVDAVKKA GGVVEATVCF 700
SGDMLQPGKK YNLDYYLEIA EKIVQMGTHI LGIKDMAGTM KPAAAKLLIG 750
SLRAKYPDLP IHVHTHDSAG TAVASMTACA LAGADVVDVA INSMSGLTSQ 800
PSINALLASL EGNIDTGINV EHVRELDAYW AEMRLLYSCF EADLKGPDPE 850
VYQHEIPGGQ LTNLLFQAQQ LGLGEQWAET KRAYREANYL LGDIVKVTPT 900
SKVVGDLAQF MVSNKLTSDD VRRLANSLDF PDSVMDFFEG LIGQPYGGFP 950
EPFRSDVLRN KRRKLTCRPG LELEPFDLEK IREDLQNRFG DVDECDVASY 1000
NMYPRVYEDF QKMRETYGDL SVLPTRSFLS PLETDEEIEV VIEQGKTLII 1050
KLQAVGDLNK KTGEREVYFD LNGEMRKIRV ADRSQKVETV TKSKADMHDP 1100
LHIGAPMAGV IVEVKVHKGS LIKKGQPVAV LSAMKMEMII SSPSDGQVKE 1150
VFVSDGENVD SSDLLVLLED QVPVETKA 1178
Length:1,178
Mass (Da):130,099
Last modified:October 1, 1996 - v2
Checksum:iBC7110A8AFB23E04
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti462 – 4621T → G in AAA34843. 1 Publication
Sequence conflicti493 – 4931V → D in AAA34843. 1 Publication
Sequence conflicti595 – 5951R → A in AAA34843. 1 Publication
Sequence conflicti619 – 6191E → Q in AAA34843. 1 Publication
Sequence conflicti664 – 6641G → S in AAA34843. 1 Publication
Sequence conflicti772 – 7721A → R in AAA34843. 1 Publication
Sequence conflicti879 – 8791E → Q in AAA34843. 1 Publication
Sequence conflicti909 – 9091Q → K in AAA34843. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03889 Genomic DNA. Translation: AAA34843.1.
Z72584 Genomic DNA. Translation: CAA96765.1.
BK006941 Genomic DNA. Translation: DAA08040.1.
PIRiS64066. QYBYP.
RefSeqiNP_011453.1. NM_001180927.1.

Genome annotation databases

EnsemblFungiiYGL062W; YGL062W; YGL062W.
GeneIDi852818.
KEGGisce:YGL062W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03889 Genomic DNA. Translation: AAA34843.1 .
Z72584 Genomic DNA. Translation: CAA96765.1 .
BK006941 Genomic DNA. Translation: DAA08040.1 .
PIRi S64066. QYBYP.
RefSeqi NP_011453.1. NM_001180927.1.

3D structure databases

ProteinModelPortali P11154.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33185. 31 interactions.
DIPi DIP-6425N.
IntActi P11154. 5 interactions.
MINTi MINT-700616.
STRINGi 4932.YGL062W.

Proteomic databases

MaxQBi P11154.
PeptideAtlasi P11154.
PRIDEi P11154.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL062W ; YGL062W ; YGL062W .
GeneIDi 852818.
KEGGi sce:YGL062W.

Organism-specific databases

CYGDi YGL062w.
SGDi S000003030. PYC1.

Phylogenomic databases

GeneTreei ENSGT00550000074986.
HOGENOMi HOG000282801.
KOi K01958.
OMAi YAIQSRV.
OrthoDBi EOG7GQZ41.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci YEAST:YGL062W-MONOMER.
BRENDAi 6.4.1.1. 984.
Reactomei REACT_188404. Defective HLCS causes multiple carboxylase deficiency.
REACT_188774. Biotin transport and metabolism.
SABIO-RKi P11154.

Miscellaneous databases

NextBioi 972360.
PROi P11154.

Gene expression databases

Genevestigatori P11154.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view ]
PANTHERi PTHR18866:SF10. PTHR18866:SF10. 1 hit.
Pfami PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001594. Pyruv_carbox. 1 hit.
SMARTi SM00878. Biotin_carb_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01235. pyruv_carbox. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and domain structure of yeast pyruvate carboxylase."
    Lim F., Morris C.P., Occhiodoro F., Wallace J.C.
    J. Biol. Chem. 263:11493-11497(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, BIOTINYLATION AT LYS-1135.
  2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1003-1178.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPYC1_YEAST
AccessioniPrimary (citable) accession number: P11154
Secondary accession number(s): D6VU79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12500 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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