Reviewed, UniProtKB/Swiss-Prot P11153 (LIPL_CAVPO)

Names and origin

Protein names

Recommended name:
    Lipoprotein lipase
      Short name=LPL
    EC=3.1.1.34

Gene names

Name:

LPL

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). The enzyme functions in the presence of apolipoprotein C-2 on the luminal surface of vascular endothelium.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subunit structure	Homodimer. Interacts with apolipoprotein C-2. Interacts with GPIHBP1.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Chylomicron Membrane VLDL
Domain	Signal
Ligand	Heparin-binding
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chylomicron Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 465	448	Lipoprotein lipase		PRO_0000017773
Regions
	Domain	331 – 454	124	PLAT
	Region	309 – 321	13	Heparin-binding Potential
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	173	1	Charge relay system By similarity
	Active site	258	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	376	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 57		By similarity
	Disulfide bond	233 ↔ 256		By similarity
	Disulfide bond	281 ↔ 300		By similarity
	Disulfide bond	292 ↔ 295		By similarity
	Disulfide bond	435 ↔ 455		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11153-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: E2515F36BEFD5F10	FASTA	465	52,781
	10 20 30 40 50 60 MNIDRKILNK ALAKEKVANC QKDYTDIESK FARRTPENTV EDTCHLIPGV TESVANCHFN 70 80 90 100 110 120 HSSKTFMVIH GWTVTGMYES WVPKLVAALY KREPDSNVIV VDWLRRAQHH YPESADYTKL 130 140 150 160 170 180 VGEDVARFIN WMEDEFKYSV DNVHLLGYSL GAHAAGVAGS RTNTKVSRIT GLDPAGPNFE 190 200 210 220 230 240 YAEATSRLSP DDAQFVDVLH TFTRGSPGRS IGIQKPVGHV DIYPNGGSFQ PGCNIQDALR 250 260 270 280 290 300 VISQKGFGDM DQLVKCSHER SIHLFIDSLL NEENPSKAYR CNSKEAFEKG LCLSCRKNRC 310 320 330 340 350 360 NNVGYEINKV RAKRSSKMYL KTRSQMPYKV FHYQVKIYFS GTETTTYTNQ AFEISLYGTV 370 380 390 400 410 420 AESENIPFTL PEVSANNTYS FLIYTEVDIG ELLMLKLKWI TESYFSWSSW WGRPTFTIEK 430 440 450 460 IRVKAGETQK KIVFCSREKV SKLQKGKEAP VFVKCHDKSL NKKSG

References

[1]	"Molecular cloning and sequence analysis of cDNA encoding lipoprotein lipase of guinea pig." Enerbaeck S., Semb H., Bengtsson-Olivecrona G., Carlsson P., Hermansson M.-L., Olivecrona T., Bjursell G. Gene 58:1-12(1987) [PubMed: 3692172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genomic organization of the region encoding guinea pig lipoprotein lipase; evidence for exon fusion and unconventional splicing." Enerbaeck S., Bjursell G. Gene 84:391-397(1989) [PubMed: 2612912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15483 mRNA. Translation: AAA37046.1. M33381 , M33377, M33378, M33379, M33380 Genomic DNA. Translation: AAA37039.1.
PIR	A27330. JS0398.
3D structure databases
HSSP	HSSP built from PDB template 1RP1 based on UniProtKB P06857.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000004098; ENSCPOP00000003655; ENSCPOG00000004054; Cavia porcellus. [Genome view] ENSCPOT00000004099; ENSCPOP00000003656; ENSCPOG00000004054; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	P11153.
Enzyme and pathway databases
BRENDA	3.1.1.34. 44.
Family and domain databases
InterPro	IPR000734. Lipase. IPR013818. Lipase_N. IPR008262. Lipase_Ser_AS. IPR002330. Lipo_Lipase. IPR001024. LipOase_LH2. IPR016272. Lipoprotein_lipase_LIPH. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11610. Lipase. 1 hit. PTHR11610:SF3. Lipase_lipo. 1 hit.
Pfam	PF00151. Lipase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PIRSF	PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTS	PR00822. LIPOLIPASE. PR00821. TAGLIPASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
TIGRFAMs	TIGR03230. lipo_lipase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPL_CAVPO

Accession

Primary (citable) accession number: P11153

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	October 13, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)