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P11076

- ARF1_YEAST

UniProt

P11076 - ARF1_YEAST

Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481GTP1 Publication
    Binding sitei70 – 701GTP; via amide nitrogen1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi25 – 328GTP1 Publication
    Nucleotide bindingi126 – 1294GTP1 Publication
    Nucleotide bindingi160 – 1612GTP1 Publication

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. Golgi to plasma membrane transport Source: SGD
    3. GTP catabolic process Source: GOC
    4. macroautophagy Source: SGD
    5. protein transport Source: UniProtKB-KW
    6. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29577-MONOMER.
    ReactomeiREACT_188955. Synthesis of PIPs at the Golgi membrane.
    REACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor 1
    Gene namesi
    Name:ARF1
    Ordered Locus Names:YDL192W
    ORF Names:D1244
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    SGDiS000002351. ARF1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207418Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine2 Publications

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    PeptideAtlasiP11076.
    PRIDEiP11076.

    Expressioni

    Gene expression databases

    GenevestigatoriP11076.

    Interactioni

    Subunit structurei

    Interacts with RUD3.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCH1Q050293EBI-2816,EBI-27508
    BCH2P361222EBI-2816,EBI-26374
    BUD7Q087542EBI-2816,EBI-32770
    CHS5Q121145EBI-2816,EBI-4640
    CHS6P409553EBI-2816,EBI-4649
    RUD3Q122345EBI-2816,EBI-31697

    Protein-protein interaction databases

    BioGridi31853. 326 interactions.
    DIPiDIP-2213N.
    IntActiP11076. 42 interactions.
    MINTiMINT-428981.
    STRINGi4932.YDL192W.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Helixi11 – 133
    Turni14 – 163
    Beta strandi19 – 235
    Turni26 – 283
    Helixi30 – 3910
    Beta strandi43 – 453
    Beta strandi49 – 5810
    Beta strandi61 – 688
    Helixi72 – 754
    Helixi76 – 783
    Helixi79 – 824
    Beta strandi86 – 938
    Beta strandi97 – 993
    Helixi100 – 11011
    Helixi114 – 1163
    Beta strandi120 – 1267
    Helixi136 – 1438
    Helixi145 – 1473
    Beta strandi153 – 1575
    Turni160 – 1634
    Helixi166 – 1749
    Turni178 – 1803

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K5UNMR-A2-181[»]
    2KSQNMR-A2-181[»]
    3TJZX-ray2.90A/D18-181[»]
    ProteinModelPortaliP11076.
    SMRiP11076. Positions 2-181.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11076.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Arf family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00720000108501.
    HOGENOMiHOG000163691.
    KOiK07937.
    OMAiQTTCATS.
    OrthoDBiEOG7FNCKB.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view]
    PfamiPF00025. Arf. 1 hit.
    [Graphical view]
    PRINTSiPR00328. SAR1GTPBP.
    SMARTiSM00177. ARF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51417. ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11076-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLFASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG    50
    FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VVDSNDRSRI 100
    GEAREVMQRM LNEDELRNAA WLVFANKQDL PEAMSAAEIT EKLGLHSIRN 150
    RPWFIQATCA TSGEGLYEGL EWLSNSLKNS T 181
    Length:181
    Mass (Da):20,529
    Last modified:January 23, 2007 - v3
    Checksum:i796859026AC63CC0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03276 Genomic DNA. Translation: AAA34431.1.
    X83276 Genomic DNA. Translation: CAA58255.1.
    Z74240 Genomic DNA. Translation: CAA98769.1.
    BK006938 Genomic DNA. Translation: DAA11671.1.
    PIRiB36167.
    RefSeqiNP_010089.1. NM_001180252.1.

    Genome annotation databases

    EnsemblFungiiYDL192W; YDL192W; YDL192W.
    GeneIDi851335.
    KEGGisce:YDL192W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03276 Genomic DNA. Translation: AAA34431.1 .
    X83276 Genomic DNA. Translation: CAA58255.1 .
    Z74240 Genomic DNA. Translation: CAA98769.1 .
    BK006938 Genomic DNA. Translation: DAA11671.1 .
    PIRi B36167.
    RefSeqi NP_010089.1. NM_001180252.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K5U NMR - A 2-181 [» ]
    2KSQ NMR - A 2-181 [» ]
    3TJZ X-ray 2.90 A/D 18-181 [» ]
    ProteinModelPortali P11076.
    SMRi P11076. Positions 2-181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31853. 326 interactions.
    DIPi DIP-2213N.
    IntActi P11076. 42 interactions.
    MINTi MINT-428981.
    STRINGi 4932.YDL192W.

    Proteomic databases

    PeptideAtlasi P11076.
    PRIDEi P11076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL192W ; YDL192W ; YDL192W .
    GeneIDi 851335.
    KEGGi sce:YDL192W.

    Organism-specific databases

    SGDi S000002351. ARF1.

    Phylogenomic databases

    GeneTreei ENSGT00720000108501.
    HOGENOMi HOG000163691.
    KOi K07937.
    OMAi QTTCATS.
    OrthoDBi EOG7FNCKB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29577-MONOMER.
    Reactomei REACT_188955. Synthesis of PIPs at the Golgi membrane.
    REACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Miscellaneous databases

    EvolutionaryTracei P11076.
    NextBioi 968402.

    Gene expression databases

    Genevestigatori P11076.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view ]
    Pfami PF00025. Arf. 1 hit.
    [Graphical view ]
    PRINTSi PR00328. SAR1GTPBP.
    SMARTi SM00177. ARF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51417. ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins."
      Sewell J., Kahn R.A.
      Proc. Natl. Acad. Sci. U.S.A. 85:4620-4624(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
      Verhasselt P., Voet M., Mathys J., Volckaert G.
      Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi."
      Gillingham A.K., Tong A.H.Y., Boone C., Munro S.
      J. Cell Biol. 167:281-292(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RUD3.
    7. "Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae."
      Trautwein M., Dengjel J., Schirle M., Spang A.
      Mol. Biol. Cell 15:5021-5037(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MRNA TRANSPORT, INTERACTION WITH ARF1.
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure and membrane interaction of myristoylated ARF1."
      Liu Y., Kahn R.A., Prestegard J.H.
      Structure 17:79-87(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GDP, MYRISTOYLATION AT GLY-2.
    10. Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GTP, MYRISTOYLATION AT GLY-2.
    11. "A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes."
      Yu X., Breitman M., Goldberg J.
      Cell 148:530-542(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 18-181.

    Entry informationi

    Entry nameiARF1_YEAST
    AccessioniPrimary (citable) accession number: P11076
    Secondary accession number(s): D6VRG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 145 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 19300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3