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P11076 (ARF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor 1
Gene names
Name:ARF1
Ordered Locus Names:YDL192W
ORF Names:D1244
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA. Ref.7

Subunit structure

Interacts with RUD3. Ref.6 Ref.7

Subcellular location

Golgi apparatus.

Miscellaneous

Present with 19300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Arf family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 181180ADP-ribosylation factor 1
PRO_0000207418

Regions

Nucleotide binding25 – 328GTP
Nucleotide binding126 – 1294GTP
Nucleotide binding160 – 1612GTP

Sites

Binding site481GTP
Binding site701GTP; via amide nitrogen

Amino acid modifications

Lipidation21N-myristoyl glycine Ref.9 Ref.10

Secondary structure

........................................... 181
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11076 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 796859026AC63CC0

FASTA18120,529
        10         20         30         40         50         60 
MGLFASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG FNVETVQYKN 

        70         80         90        100        110        120 
ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VVDSNDRSRI GEAREVMQRM LNEDELRNAA 

       130        140        150        160        170        180 
WLVFANKQDL PEAMSAAEIT EKLGLHSIRN RPWFIQATCA TSGEGLYEGL EWLSNSLKNS 


T 

« Hide

References

« Hide 'large scale' references
[1]"Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins."
Sewell J., Kahn R.A.
Proc. Natl. Acad. Sci. U.S.A. 85:4620-4624(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
Verhasselt P., Voet M., Mathys J., Volckaert G.
Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi."
Gillingham A.K., Tong A.H.Y., Boone C., Munro S.
J. Cell Biol. 167:281-292(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RUD3.
[7]"Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae."
Trautwein M., Dengjel J., Schirle M., Spang A.
Mol. Biol. Cell 15:5021-5037(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MRNA TRANSPORT, INTERACTION WITH ARF1.
[8]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structure and membrane interaction of myristoylated ARF1."
Liu Y., Kahn R.A., Prestegard J.H.
Structure 17:79-87(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GDP, MYRISTOYLATION AT GLY-2.
[10]"Dynamic structure of membrane-anchored Arf*GTP."
Liu Y., Kahn R.A., Prestegard J.H.
Nat. Struct. Mol. Biol. 17:876-881(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GTP, MYRISTOYLATION AT GLY-2.
[11]"A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes."
Yu X., Breitman M., Goldberg J.
Cell 148:530-542(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 18-181.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03276 Genomic DNA. Translation: AAA34431.1.
X83276 Genomic DNA. Translation: CAA58255.1.
Z74240 Genomic DNA. Translation: CAA98769.1.
BK006938 Genomic DNA. Translation: DAA11671.1.
PIRB36167.
RefSeqNP_010089.1. NM_001180252.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2K5UNMR-A2-181[»]
2KSQNMR-A2-181[»]
3TJZX-ray2.90A/D18-181[»]
ProteinModelPortalP11076.
SMRP11076. Positions 2-181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31853. 326 interactions.
DIPDIP-2213N.
IntActP11076. 42 interactions.
MINTMINT-428981.
STRING4932.YDL192W.

Proteomic databases

PeptideAtlasP11076.
PRIDEP11076.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL192W; YDL192W; YDL192W.
GeneID851335.
KEGGsce:YDL192W.

Organism-specific databases

SGDS000002351. ARF1.

Phylogenomic databases

GeneTreeENSGT00720000108501.
HOGENOMHOG000163691.
KOK07937.
OMAQTTCATS.
OrthoDBEOG7FNCKB.

Enzyme and pathway databases

BioCycYEAST:G3O-29577-MONOMER.

Gene expression databases

GenevestigatorP11076.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamPF00025. Arf. 1 hit.
[Graphical view]
PRINTSPR00328. SAR1GTPBP.
SMARTSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP11076.
NextBio968402.

Entry information

Entry nameARF1_YEAST
AccessionPrimary (citable) accession number: P11076
Secondary accession number(s): D6VRG1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references