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P11076

- ARF1_YEAST

UniProt

P11076 - ARF1_YEAST

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Protein

ADP-ribosylation factor 1

Gene
ARF1, YDL192W, D1244
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481GTP
Binding sitei70 – 701GTP; via amide nitrogen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328GTP
Nucleotide bindingi126 – 1294GTP
Nucleotide bindingi160 – 1612GTP

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW
  3. protein binding Source: IntAct

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. Golgi to plasma membrane transport Source: SGD
  3. GTP catabolic process Source: GOC
  4. macroautophagy Source: SGD
  5. protein transport Source: UniProtKB-KW
  6. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29577-MONOMER.
ReactomeiREACT_188955. Synthesis of PIPs at the Golgi membrane.
REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
Ordered Locus Names:YDL192W
ORF Names:D1244
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

SGDiS000002351. ARF1.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine2 Publications

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PeptideAtlasiP11076.
PRIDEiP11076.

Expressioni

Gene expression databases

GenevestigatoriP11076.

Interactioni

Subunit structurei

Interacts with RUD3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCH1Q050293EBI-2816,EBI-27508
BCH2P361222EBI-2816,EBI-26374
BUD7Q087542EBI-2816,EBI-32770
CHS5Q121145EBI-2816,EBI-4640
CHS6P409553EBI-2816,EBI-4649
RUD3Q122345EBI-2816,EBI-31697

Protein-protein interaction databases

BioGridi31853. 326 interactions.
DIPiDIP-2213N.
IntActiP11076. 42 interactions.
MINTiMINT-428981.
STRINGi4932.YDL192W.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85
Helixi11 – 133
Turni14 – 163
Beta strandi19 – 235
Turni26 – 283
Helixi30 – 3910
Beta strandi43 – 453
Beta strandi49 – 5810
Beta strandi61 – 688
Helixi72 – 754
Helixi76 – 783
Helixi79 – 824
Beta strandi86 – 938
Beta strandi97 – 993
Helixi100 – 11011
Helixi114 – 1163
Beta strandi120 – 1267
Helixi136 – 1438
Helixi145 – 1473
Beta strandi153 – 1575
Turni160 – 1634
Helixi166 – 1749
Turni178 – 1803

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K5UNMR-A2-181[»]
2KSQNMR-A2-181[»]
3TJZX-ray2.90A/D18-181[»]
ProteinModelPortaliP11076.
SMRiP11076. Positions 2-181.

Miscellaneous databases

EvolutionaryTraceiP11076.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00720000108501.
HOGENOMiHOG000163691.
KOiK07937.
OMAiQTTCATS.
OrthoDBiEOG7FNCKB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11076-1 [UniParc]FASTAAdd to Basket

« Hide

MGLFASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG    50
FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VVDSNDRSRI 100
GEAREVMQRM LNEDELRNAA WLVFANKQDL PEAMSAAEIT EKLGLHSIRN 150
RPWFIQATCA TSGEGLYEGL EWLSNSLKNS T 181
Length:181
Mass (Da):20,529
Last modified:January 23, 2007 - v3
Checksum:i796859026AC63CC0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03276 Genomic DNA. Translation: AAA34431.1.
X83276 Genomic DNA. Translation: CAA58255.1.
Z74240 Genomic DNA. Translation: CAA98769.1.
BK006938 Genomic DNA. Translation: DAA11671.1.
PIRiB36167.
RefSeqiNP_010089.1. NM_001180252.1.

Genome annotation databases

EnsemblFungiiYDL192W; YDL192W; YDL192W.
GeneIDi851335.
KEGGisce:YDL192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03276 Genomic DNA. Translation: AAA34431.1 .
X83276 Genomic DNA. Translation: CAA58255.1 .
Z74240 Genomic DNA. Translation: CAA98769.1 .
BK006938 Genomic DNA. Translation: DAA11671.1 .
PIRi B36167.
RefSeqi NP_010089.1. NM_001180252.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2K5U NMR - A 2-181 [» ]
2KSQ NMR - A 2-181 [» ]
3TJZ X-ray 2.90 A/D 18-181 [» ]
ProteinModelPortali P11076.
SMRi P11076. Positions 2-181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31853. 326 interactions.
DIPi DIP-2213N.
IntActi P11076. 42 interactions.
MINTi MINT-428981.
STRINGi 4932.YDL192W.

Proteomic databases

PeptideAtlasi P11076.
PRIDEi P11076.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL192W ; YDL192W ; YDL192W .
GeneIDi 851335.
KEGGi sce:YDL192W.

Organism-specific databases

SGDi S000002351. ARF1.

Phylogenomic databases

GeneTreei ENSGT00720000108501.
HOGENOMi HOG000163691.
KOi K07937.
OMAi QTTCATS.
OrthoDBi EOG7FNCKB.

Enzyme and pathway databases

BioCyci YEAST:G3O-29577-MONOMER.
Reactomei REACT_188955. Synthesis of PIPs at the Golgi membrane.
REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Miscellaneous databases

EvolutionaryTracei P11076.
NextBioi 968402.

Gene expression databases

Genevestigatori P11076.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins."
    Sewell J., Kahn R.A.
    Proc. Natl. Acad. Sci. U.S.A. 85:4620-4624(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
    Verhasselt P., Voet M., Mathys J., Volckaert G.
    Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi."
    Gillingham A.K., Tong A.H.Y., Boone C., Munro S.
    J. Cell Biol. 167:281-292(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RUD3.
  7. "Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae."
    Trautwein M., Dengjel J., Schirle M., Spang A.
    Mol. Biol. Cell 15:5021-5037(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MRNA TRANSPORT, INTERACTION WITH ARF1.
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structure and membrane interaction of myristoylated ARF1."
    Liu Y., Kahn R.A., Prestegard J.H.
    Structure 17:79-87(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GDP, MYRISTOYLATION AT GLY-2.
  10. Cited for: STRUCTURE BY NMR OF 2-181 IN COMPLEX WITH GTP, MYRISTOYLATION AT GLY-2.
  11. "A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes."
    Yu X., Breitman M., Goldberg J.
    Cell 148:530-542(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 18-181.

Entry informationi

Entry nameiARF1_YEAST
AccessioniPrimary (citable) accession number: P11076
Secondary accession number(s): D6VRG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 19300 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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