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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48GTP1 Publication1
Binding sitei70GTP; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32GTP1 Publication8
Nucleotide bindingi126 – 129GTP1 Publication4
Nucleotide bindingi160 – 161GTP1 Publication2

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • Golgi to plasma membrane transport Source: SGD
  • macroautophagy Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29577-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
Ordered Locus Names:YDL192W
ORF Names:D1244
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL192W.
SGDiS000002351. ARF1.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002074182 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1
Cross-linki127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiP11076.
PRIDEiP11076.
TopDownProteomicsiP11076.

Interactioni

Subunit structurei

Interacts with RUD3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCH1Q050293EBI-2816,EBI-27508
BCH2P361222EBI-2816,EBI-26374
BUD7Q087542EBI-2816,EBI-32770
CHS5Q121145EBI-2816,EBI-4640
CHS6P409553EBI-2816,EBI-4649
RUD3Q122345EBI-2816,EBI-31697

Protein-protein interaction databases

BioGridi31853. 333 interactors.
DIPiDIP-2213N.
IntActiP11076. 42 interactors.
MINTiMINT-428981.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi11 – 13Combined sources3
Turni14 – 16Combined sources3
Beta strandi19 – 23Combined sources5
Turni26 – 28Combined sources3
Helixi30 – 39Combined sources10
Beta strandi43 – 45Combined sources3
Beta strandi49 – 58Combined sources10
Beta strandi61 – 68Combined sources8
Helixi72 – 75Combined sources4
Helixi76 – 78Combined sources3
Helixi79 – 82Combined sources4
Beta strandi86 – 93Combined sources8
Beta strandi97 – 99Combined sources3
Helixi100 – 110Combined sources11
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 174Combined sources9
Turni178 – 180Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K5UNMR-A2-181[»]
2KSQNMR-A2-181[»]
3TJZX-ray2.90A/D18-181[»]
4Q66X-ray3.35C/F/I/L18-181[»]
5A1Uelectron microscopy13.00A/B1-181[»]
5A1Velectron microscopy21.00A/B/I/J/R/S1-181[»]
5A1Welectron microscopy18.00A/B1-181[»]
5A1Xelectron microscopy23.00A/B/I/J1-181[»]
5A1Yelectron microscopy21.00A/B/I/J/R1-181[»]
ProteinModelPortaliP11076.
SMRiP11076.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11076.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129731.
HOGENOMiHOG000163691.
InParanoidiP11076.
KOiK07937.
OMAiHYFRNTE.
OrthoDBiEOG092C508L.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLFASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG
60 70 80 90 100
FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VVDSNDRSRI
110 120 130 140 150
GEAREVMQRM LNEDELRNAA WLVFANKQDL PEAMSAAEIT EKLGLHSIRN
160 170 180
RPWFIQATCA TSGEGLYEGL EWLSNSLKNS T
Length:181
Mass (Da):20,529
Last modified:January 23, 2007 - v3
Checksum:i796859026AC63CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03276 Genomic DNA. Translation: AAA34431.1.
X83276 Genomic DNA. Translation: CAA58255.1.
Z74240 Genomic DNA. Translation: CAA98769.1.
BK006938 Genomic DNA. Translation: DAA11671.1.
PIRiB36167.
RefSeqiNP_010089.1. NM_001180252.1.

Genome annotation databases

EnsemblFungiiYDL192W; YDL192W; YDL192W.
GeneIDi851335.
KEGGisce:YDL192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03276 Genomic DNA. Translation: AAA34431.1.
X83276 Genomic DNA. Translation: CAA58255.1.
Z74240 Genomic DNA. Translation: CAA98769.1.
BK006938 Genomic DNA. Translation: DAA11671.1.
PIRiB36167.
RefSeqiNP_010089.1. NM_001180252.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K5UNMR-A2-181[»]
2KSQNMR-A2-181[»]
3TJZX-ray2.90A/D18-181[»]
4Q66X-ray3.35C/F/I/L18-181[»]
5A1Uelectron microscopy13.00A/B1-181[»]
5A1Velectron microscopy21.00A/B/I/J/R/S1-181[»]
5A1Welectron microscopy18.00A/B1-181[»]
5A1Xelectron microscopy23.00A/B/I/J1-181[»]
5A1Yelectron microscopy21.00A/B/I/J/R1-181[»]
ProteinModelPortaliP11076.
SMRiP11076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31853. 333 interactors.
DIPiDIP-2213N.
IntActiP11076. 42 interactors.
MINTiMINT-428981.

Proteomic databases

MaxQBiP11076.
PRIDEiP11076.
TopDownProteomicsiP11076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL192W; YDL192W; YDL192W.
GeneIDi851335.
KEGGisce:YDL192W.

Organism-specific databases

EuPathDBiFungiDB:YDL192W.
SGDiS000002351. ARF1.

Phylogenomic databases

GeneTreeiENSGT00840000129731.
HOGENOMiHOG000163691.
InParanoidiP11076.
KOiK07937.
OMAiHYFRNTE.
OrthoDBiEOG092C508L.

Enzyme and pathway databases

BioCyciYEAST:G3O-29577-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP11076.
PROiP11076.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_YEAST
AccessioniPrimary (citable) accession number: P11076
Secondary accession number(s): D6VRG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 19300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.