P11072 (LIT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bacteriophage T4 late gene expression-blocking protein
Short name=GpLit

Gene names

Name:	lit
Ordered Locus Names:	b1139, JW1125

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interacts with a short DNA sequence about one-quarter of the way into the major capsid protein gene 23 of T4, and the inhibition occurs when this late gene of the virus is expressed.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the peptidase U49 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational elongation Inferred from direct assay PubMed 9501186. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidase activity Inferred from direct assay PubMed 9501186. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Bacteriophage T4 late gene expression-blocking protein		PRO_0000084446
Regions
	Transmembrane	61 – 82	22	Helical; Potential
	Transmembrane	149 – 178	30	Helical; Potential
Experimental info
	Sequence conflict	121 – 135	15	TGCES…PKPEA → QVANHGLKNVPSQKH in AAA24074. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11072 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8A06C0EB82FBB1AF	FASTA	297	33,762
	10 20 30 40 50 60 MRSPICHLFS AINSSPFKIA PEKEQDLKTI VDDKKIIISV VSEPGFNIRV RKNESNNSHE 70 80 90 100 110 120 IVLTVASLEY IWAFSNFFWV FTQEYSKSQK NNDEHFDLTG KNRLKKSDEL LKWARKNLQT 130 140 150 160 170 180 TGCESWPKKC PKPEAYLQGS EDSQVASEIF LCAIAWILHH EISHVVLQHP LVTTAFSTQE 190 200 210 220 230 240 EREADSHATK WILGNLYESA PELKKRALGI ATAVLCIQSL EVENYFCLQN THPAAYERIY 250 260 270 280 290 SNISCYPVGN EELIEALCTV MLQYLFHGKN INVNLDGESF SSILGDLLCD ISRLTSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The lit gene product which blocks bacteriophage T4 late gene expression is a membrane protein encoded by a cryptic DNA element, e14." Kao C., Snyder L. J. Bacteriol. 170:2056-2062(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / JM101 / ATCC 33876 / DSM 3948 / NCIMB 11926.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19634 Genomic DNA. Translation: AAA24074.1. U00096 Genomic DNA. Translation: AAC74223.1. AP009048 Genomic DNA. Translation: BAA35968.1.
PIR	BVECLT. H64858.
RefSeq	NP_415657.1. NC_000913.2. YP_489407.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P11072.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1139.
Protein family/group databases
MEROPS	U49.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74223; AAC74223; b1139. BAA35968; BAA35968; BAA35968.
GeneID	12934105. 948421.
KEGG	ecj:Y75_p1109. eco:b1139.
PATRIC	32117525. VBIEscCol129921_1185.
Organism-specific databases
EchoBASE	EB0530.
EcoGene	EG10535. lit.
Phylogenomic databases
eggNOG	NOG86897.
OMA	APENESK.
ProtClustDB	PRK09672.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10535-MONOMER. ECOL316407:JW1125-MONOMER.
Gene expression databases
Genevestigator	P11072.
Family and domain databases
InterPro	IPR019504. Peptidase_U49_Lit_pept. [Graphical view]
Pfam	PF10463. Peptidase_U49. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIT_ECOLI

Accession

Primary (citable) accession number: P11072
Secondary accession number(s): P77283

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program