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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei336 – 3361ATPUniRule annotation
Active sitei371 – 3711Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi315 – 3217ATPUniRule annotation

GO - Molecular functioni

  • [isocitrate dehydrogenase (NADP+)] kinase activity Source: EcoCyc
  • [isocitrate dehydrogenase (NADP+)] phosphatase activity Source: UniProtKB-EC
  • AMP binding Source: EcoCyc
  • ATP binding Source: EcoliWiki
  • protein serine/threonine kinase activity Source: EcoCyc
  • protein serine/threonine phosphatase activity Source: EcoCyc

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • glyoxylate cycle Source: EcoliWiki
  • peptidyl-serine phosphorylation Source: EcoCyc
  • protein dephosphorylation Source: GOC
  • regulation of catalytic activity Source: EcoCyc
  • tricarboxylic acid cycle Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ICITDEHASE-KIN-PHOSPHA.
ECOL316407:JW3976-MONOMER.
MetaCyc:ICITDEHASE-KIN-PHOSPHA.
BRENDAi2.7.11.5. 2026.
SABIO-RKP11071.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:b4016, JW3976
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10026. aceK.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi336 – 3361K → M: Inhibits enzyme. 1 Publication
Mutagenesisi371 – 3711D → A, E or Q: Loss of activity. 1 Publication
Mutagenesisi377 – 3771N → A: No loss of activity. 1 Publication
Mutagenesisi403 – 4031D → A: No loss of activity. 1 Publication
Mutagenesisi439 – 4391E → A: No loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Isocitrate dehydrogenase kinase/phosphatasePRO_0000057898Add
BLAST

Proteomic databases

PaxDbiP11071.

Interactioni

Protein-protein interaction databases

BioGridi4261467. 10 interactions.
DIPiDIP-9041N.
IntActiP11071. 12 interactions.
MINTiMINT-1711235.
STRINGi511145.b4016.

Structurei

3D structure databases

ProteinModelPortaliP11071.
SMRiP11071. Positions 2-574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
InParanoidiP11071.
KOiK00906.
OMAiEPWYSVG.
OrthoDBiEOG6HQSJX.

Family and domain databases

HAMAPiMF_00747. AceK.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P11071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE
160 170 180 190 200
SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VRYGEMLF
Length:578
Mass (Da):67,699
Last modified:December 1, 2000 - v2
Checksum:i5ABD5FB5FA1D9B29
GO

Sequence cautioni

The sequence AAA24007.1 differs from that shown. Reason: Frameshift at positions 92, 137 and 166. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401A → G in AAA24007 (PubMed:2826408).Curated
Sequence conflicti416 – 4172ER → DG in AAA24010 (PubMed:2836370).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20714 Genomic DNA. Translation: AAA24010.1.
M18974 Genomic DNA. Translation: AAA24007.1. Frameshift.
U00006 Genomic DNA. Translation: AAC43110.1.
U00096 Genomic DNA. Translation: AAC76986.1.
AP009048 Genomic DNA. Translation: BAE78018.1.
M22621 Genomic DNA. Translation: AAC13651.1.
M63497 Genomic DNA. Translation: AAA73005.1.
PIRiG65208. KIECID.
RefSeqiNP_418440.1. NC_000913.3.
WP_001137220.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76986; AAC76986; b4016.
BAE78018; BAE78018; BAE78018.
GeneIDi944797.
KEGGiecj:JW3976.
eco:b4016.
PATRICi32123561. VBIEscCol129921_4129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20714 Genomic DNA. Translation: AAA24010.1.
M18974 Genomic DNA. Translation: AAA24007.1. Frameshift.
U00006 Genomic DNA. Translation: AAC43110.1.
U00096 Genomic DNA. Translation: AAC76986.1.
AP009048 Genomic DNA. Translation: BAE78018.1.
M22621 Genomic DNA. Translation: AAC13651.1.
M63497 Genomic DNA. Translation: AAA73005.1.
PIRiG65208. KIECID.
RefSeqiNP_418440.1. NC_000913.3.
WP_001137220.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP11071.
SMRiP11071. Positions 2-574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261467. 10 interactions.
DIPiDIP-9041N.
IntActiP11071. 12 interactions.
MINTiMINT-1711235.
STRINGi511145.b4016.

Proteomic databases

PaxDbiP11071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76986; AAC76986; b4016.
BAE78018; BAE78018; BAE78018.
GeneIDi944797.
KEGGiecj:JW3976.
eco:b4016.
PATRICi32123561. VBIEscCol129921_4129.

Organism-specific databases

EchoBASEiEB0025.
EcoGeneiEG10026. aceK.

Phylogenomic databases

eggNOGiENOG4105VS7. Bacteria.
COG4579. LUCA.
HOGENOMiHOG000247673.
InParanoidiP11071.
KOiK00906.
OMAiEPWYSVG.
OrthoDBiEOG6HQSJX.

Enzyme and pathway databases

BioCyciEcoCyc:ICITDEHASE-KIN-PHOSPHA.
ECOL316407:JW3976-MONOMER.
MetaCyc:ICITDEHASE-KIN-PHOSPHA.
BRENDAi2.7.11.5. 2026.
SABIO-RKP11071.

Miscellaneous databases

PROiP11071.

Family and domain databases

HAMAPiMF_00747. AceK.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase."
    Klumpp D.J., Plank D.W., Bowdin L.J., Stueland C.S., Chung T., Laporte D.C.
    J. Bacteriol. 170:2763-2769(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli."
    Cortay J.-C., Bleicher F., Rieul C., Reeves H.C., Cozzone A.J.
    J. Bacteriol. 170:89-97(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli."
    Matsuoka M., McFadden B.A.
    J. Bacteriol. 170:4528-4536(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
  7. "Primary structure of the intergenic region between aceK and iclR in the Escherichia coli chromosome."
    Galinier A., Bleicher F., Negre D., Perriere G., Duclos B., Cozzone A.J., Cortay J.-C.
    Gene 97:149-150(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 552-578.
  8. "Isocitrate dehydrogenase kinase/phosphatase."
    Laporte D.C., Stueland C.S., Ikeda T.P.
    Biochimie 71:1051-1057(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, MUTAGENESIS OF LYS-336.
  9. "The 'catalytic' triad of isocitrate dehydrogenase kinase/phosphatase from E. coli and its relationship with that found in eukaryotic protein kinases."
    Oudot C., Cortay J.-C., Blanchet C., Laporte D.C., Di Pietro A., Cozzone A.J., Jault J.-M.
    Biochemistry 40:3047-3055(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-371; ASN-377; ASP-403 AND GLU-439.

Entry informationi

Entry nameiACEK_ECOLI
AccessioniPrimary (citable) accession number: P11071
Secondary accession number(s): P11070, Q2M6T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.