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Reviewed, UniProtKB/Swiss-Prot P11071 (ACEK_ECOLI)

Last modified June 15, 2010. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Isocitrate dehydrogenase kinase/phosphatase

Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-
Gene names
Name:aceK
Ordered Locus Names:b4016, JW3976
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
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Protein attributesHide

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation. HAMAP MF_00747

Catalytic activity

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747

Subcellular location

Cytoplasm HAMAP MF_00747.

Sequence similarities

Belongs to the AceK family.

Sequence caution

The sequence AAA24007.1 differs from that shown. Reason: Frameshift at positions 92, 137 and 166.

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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747
PRO_0000057898

Regions

Nucleotide binding315 – 3217ATP By similarity

Sites

Active site3711 Probable
Binding site3361ATP By similarity

Experimental info

Mutagenesis3361K → M: Inhibits enzyme. Ref.8
Mutagenesis3711D → A, E or Q: Loss of activity. Ref.9
Mutagenesis3771N → A: No loss of activity. Ref.9
Mutagenesis4031D → A: No loss of activity. Ref.9
Mutagenesis4391E → A: No loss of activity. Ref.9
Sequence conflict1401A → G in AAA24007. Ref.2
Sequence conflict416 – 4172ER → DG in AAA24010. Ref.1
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SequencesHide

Sequence LengthMass (Da)Tools
P11071-1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 5ABD5FB5FA1D9B29

FASTA57867,699
        10         20         30         40         50         60 
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR IHLYDHHVGL 

        70         80         90        100        110        120 
VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER 

       130        140        150        160        170        180 
LFIFSSQPER RFRTIPRPLA KDFHPDHGWE SLLMRVISDL PLRLRWQNKS RDIHYIIRHL 

       190        200        210        220        230        240 
TETLGTDNLA ESHLQVANEL FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL 

       250        260        270        280        290        300 
TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 

       310        320        330        340        350        360 
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA AHVRACYQLV 

       370        380        390        400        410        420 
KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK ITDLGEQIVI RHLYIERRMV 

       430        440        450        460        470        480 
PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 

       490        500        510        520        530        540 
YMTEVNFRDI PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF 

       550        560        570 
RADYWRALQN RIREGHVEDV YAYRRRQRFS VRYGEMLF 

« Hide

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ReferencesHide

« Hide 'large scale' references
[1]"Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase."
Klumpp D.J., Plank D.W., Bowdin L.J., Stueland C.S., Chung T., Laporte D.C.
J. Bacteriol. 170:2763-2769(1988) [PubMed: 2836370] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli."
Cortay J.-C., Bleicher F., Rieul C., Reeves H.C., Cozzone A.J.
J. Bacteriol. 170:89-97(1988) [PubMed: 2826408] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli."
Matsuoka M., McFadden B.A.
J. Bacteriol. 170:4528-4536(1988) [PubMed: 3049537] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
[7]"Primary structure of the intergenic region between aceK and iclR in the Escherichia coli chromosome."
Galinier A., Bleicher F., Negre D., Perriere G., Duclos B., Cozzone A.J., Cortay J.-C.
Gene 97:149-150(1991) [PubMed: 1995429] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 552-578.
[8]"Isocitrate dehydrogenase kinase/phosphatase."
Laporte D.C., Stueland C.S., Ikeda T.P.
Biochimie 71:1051-1057(1989) [PubMed: 2557093] [Abstract]
Cited for: REVIEW, MUTAGENESIS OF LYS-336.
[9]"The 'catalytic' triad of isocitrate dehydrogenase kinase/phosphatase from E. coli and its relationship with that found in eukaryotic protein kinases."
Oudot C., Cortay J.-C., Blanchet C., Laporte D.C., Di Pietro A., Cozzone A.J., Jault J.-M.
Biochemistry 40:3047-3055(2001) [PubMed: 11258918] [Abstract]
Cited for: MUTAGENESIS OF ASP-371; ASN-377; ASP-403 AND GLU-439.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ
M20714 Genomic DNA. Translation: AAA24010.1.
M18974 Genomic DNA. Translation: AAA24007.1. Frameshift.
U00006 Genomic DNA. Translation: AAC43110.1.
U00096 Genomic DNA. Translation: AAC76986.1.
AP009048 Genomic DNA. Translation: BAE78018.1.
M22621 Genomic DNA. Translation: AAC13651.1.
M63497 Genomic DNA. Translation: AAA73005.1.
PIRKIECID. G65208.
RefSeqAP_004517.1.
NP_418440.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP11071.

Protein family/group databases

PptaseDBP3D0409136.

Genome annotation databases

EnsemblBacteriaEBESCT00000002125; EBESCP00000002125; EBESCG00000001741.
EBESCT00000016793; EBESCP00000016084; EBESCG00000015852.
GeneID944797.
GenomeReviewsGene locus JW3976 in contig AP009048_GR.
Gene locus b4016 in contig U00096_GR.
KEGGecj:JW3976.
eco:b4016.

Organism-specific databases

EchoBASEEB0025.
EcoGeneEG10026. aceK.
CMRSearch...

Phylogenomic databases

eggNOGCOG4579.
HOGENOMHBG298593.
OMARMTPLNI.
ProtClustDBPRK02946.

Enzyme and pathway databases

BioCycEcoCyc:ICITDEHASE-KIN-PHOSPHA.
ECOL168927:B4016-MONOMER.
MetaCyc:ICITDEHASE-KIN-PHOSPHA.

Gene expression databases

GenevestigatorP11071.

Family and domain databases

HAMAPMF_00747. AceK.
[Tree]
InterProIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamPF06315. AceK. 1 hit.
[Graphical view]
PIRSFPIRSF000719. AceK. 1 hit.
ProDomPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...
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Entry informationHide

Entry nameACEK_ECOLI
AccessionPrimary (citable) accession number: P11071
Secondary accession number(s): P11070, Q2M6T8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 1, 2000
Last modified: June 15, 2010
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents