P11058 (TXMG2_AGEAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Mu-agatoxin-Aa1b Short name=Mu-AGTX-Aa1b Alternative name(s): Mu-agatoxin II Mu-agatoxin-2
Organism	Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifier	6908 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › RTA clade › Agelenidae › Agelenopsis

Protein attributes

Sequence length	37 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
Toxic dose	Lethal dose is 75 +-27 mg/kg into third stadium larvae of M.sexta. Ref.1
Sequence similarities	Belongs to the beta/delta-agatoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Ion channel impairing toxin Neurotoxin Presynaptic neurotoxin Sodium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	other organism presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 37	37	Mu-agatoxin-Aa1b		PRO_0000044954
Amino acid modifications
	Modified residue	37	1	Serine amide Probable
	Disulfide bond	2 ↔ 18		By similarity
	Disulfide bond	9 ↔ 23		By similarity
	Disulfide bond	17 ↔ 33		By similarity
	Disulfide bond	25 ↔ 31		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11058 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8900D367B4F096CD	FASTA	37	4,110
	10 20 30 ECATKNKRCA DWAGPWCCDG LYCSCRSYPG CMCRPSS

References

[1]	"Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta." Skinner W.S., Adams M.E., Quistad G.B., Kataoka H., Cesarin B.J., Enderlin F.E., Schooley D.A. J. Biol. Chem. 264:2150-2155(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, LETHAL DOSE. Tissue: Venom.
[2]	"Agatoxins: ion channel specific toxins from the American funnel web spider, Agelenopsis aperta." Adams M.E. Toxicon 43:509-525(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
PIR	B32038.
3D structure databases
ProteinModelPortal	P11058.
SMR	P11058. Positions 1-37.
ModBase	Search...
Protein family/group databases
TCDB	8.B.6.2.2. Ca2+ channel-targeting spider toxin (CST) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ArachnoServer	AS000377. mu-agatoxin-Aa1b.
Family and domain databases
InterPro	IPR016328. Curtatoxin. IPR009243. Toxin_7. [Graphical view]
PIRSF	PIRSF001882. Curtatoxin. 1 hit.
PROSITE	PS60015. MU_AGATOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TXMG2_AGEAP

Accession

Primary (citable) accession number: P11058

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program