P11057 (TXMG1_AGEAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Mu-agatoxin-Aa1a Short name=Mu-AGTX-Aa1a Alternative name(s): Mu-agatoxin I Mu-agatoxin-1
Organism	Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifier	6908 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › RTA clade › Agelenidae › Agelenopsis

Protein attributes

Sequence length	36 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Toxic dose	Lethal dose is 28 +-7 mg/kg into third stadium larvae of M.sexta. Ref.1
Sequence similarities	Belongs to the beta/delta-agatoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Ion channel impairing toxin Neurotoxin Presynaptic neurotoxin Sodium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	other organism presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 36

36

Mu-agatoxin-Aa1a

PRO_0000044952

Amino acid modifications

Modified residue

36

1

Asparagine amide Ref.1 Ref.2

Disulfide bond

2 ↔ 17

Ref.2

Disulfide bond

9 ↔ 22

Ref.2

Disulfide bond

16 ↔ 32

Ref.2

Disulfide bond

24 ↔ 30

Ref.2

Secondary structure

1

.

.

.

.

.

.

.

36

Helix Strand Turn

Details...

Beta strand

11 – 14

4

Beta strand

20 – 26

7

Beta strand

29 – 34

6

Sequences

	Sequence		Length	Mass (Da)
	P11057 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 3B973A605B90DE85	FASTA	36	4,273
	10 20 30 ECVPENGHCR DWYDECCEGF YCSCRQPPKC ICRNNN

References

[1]	"Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta." Skinner W.S., Adams M.E., Quistad G.B., Kataoka H., Cesarin B.J., Enderlin F.E., Schooley D.A. J. Biol. Chem. 264:2150-2155(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, LETHAL DOSE. Tissue: Venom.
[2]	"Three-dimensional structure analysis of mu-agatoxins: further evidence for common motifs among neurotoxins with diverse ion channel specificities." Omecinsky D.O., Holub K.E., Adams M.E., Reily M.D. Biochemistry 35:2836-2844(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, AMIDATION AT ASN-36, DISULFIDE BONDS.
[3]	"Agatoxins: ion channel specific toxins from the American funnel web spider, Agelenopsis aperta." Adams M.E. Toxicon 43:509-525(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases

PIR

A32038.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EIT	NMR	-	A	1-36	[»]

ProteinModelPortal

P11057.

SMR

P11057. Positions 1-36.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

ArachnoServer

AS000375. mu-agatoxin-Aa1a.

Family and domain databases

InterPro

IPR016328. Curtatoxin.
IPR009243. Toxin_7.
[Graphical view]

PIRSF

PIRSF001882. Curtatoxin. 1 hit.

PROSITE

PS60015. MU_AGATOXIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P11057.

Entry information

Entry name

TXMG1_AGEAP

Accession

Primary (citable) accession number: P11057

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program