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Protein

Dihydrofolate reductase

Gene

dfrA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (dfrA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 7NADPBy similarity2
Nucleotide bindingi14 – 19NADPBy similarity6
Nucleotide bindingi43 – 46NADPBy similarity4
Nucleotide bindingi62 – 65NADPBy similarity4
Nucleotide bindingi95 – 100NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciBSUB:BSU21810-MONOMER
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:dfrA
Ordered Locus Names:BSU21810
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863821 – 168Dihydrofolate reductaseAdd BLAST168

Proteomic databases

PaxDbiP11045

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012011

Structurei

3D structure databases

ProteinModelPortaliP11045
SMRiP11045
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 159DHFRPROSITE-ProRule annotationAdd BLAST159

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 7Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108YYV Bacteria
COG0262 LUCA
HOGENOMiHOG000040233
InParanoidiP11045
KOiK00287
OMAiEECFVIG
PhylomeDBiP11045

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISFIFAMDA NRLIGKDNDL PWHLPNDLAY FKKITSGHSI IMGRKTFESI
60 70 80 90 100
GRPLPNRKNI VVTSAPDSEF QGCTVVSSLK DVLDICSGPE ECFVIGGAQL
110 120 130 140 150
YTDLFPYADR LYMTKIHHEF EGDRHFPEFD ESNWKLVSSE QGTKDEKNPY
160
DYEFLMYEKK NSSKAGGF
Length:168
Mass (Da):19,176
Last modified:October 1, 1996 - v2
Checksum:i6E4D11D03F630A44
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti165A → V in strain: MI112. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20012 Genomic DNA Translation: AAA22853.1
L77246 Genomic DNA Translation: AAA96635.1
AL009126 Genomic DNA Translation: CAB14099.1
PIRiJT0291 RDBSD
RefSeqiNP_390064.1, NC_000964.3
WP_003230799.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14099; CAB14099; BSU21810
GeneIDi939091
KEGGibsu:BSU21810
PATRICifig|224308.179.peg.2383

Similar proteinsi

Entry informationi

Entry nameiDYR_BACSU
AccessioniPrimary (citable) accession number: P11045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health