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Protein

Thymidylate synthase 2

Gene

thyA2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Temperature dependencei

Thermolabile. Inactive at 46 degrees Celsius.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei146NucleophileUniRule annotation1
Binding sitei1695,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei177dUMPUniRule annotation1
Binding sitei2635,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi166 – 169dUMPUniRule annotation4
Nucleotide bindingi207 – 209dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU21820-MONOMER.
SABIO-RKP11044.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 2UniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TS 2UniRule annotation
Short name:
TSase 2UniRule annotation
Alternative name(s):
Thymidylate synthase A1 Publication
Short name:
TS A1 Publication
Short name:
TSase A1 Publication
Gene namesi
Name:thyA2UniRule annotation
Synonyms:thyB
Ordered Locus Names:BSU21820
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409321 – 264Thymidylate synthase 2Add BLAST264

Proteomic databases

PaxDbiP11044.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP11044. 6 interactors.
STRINGi224308.Bsubs1_010100012016.

Structurei

3D structure databases

ProteinModelPortaliP11044.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
InParanoidiP11044.
KOiK00560.
OMAiIVYELLW.
PhylomeDBiP11044.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYKDFCRH VLEHGEKKGD RTGTGTISTF GYQMRFNLRE GFPMLTTKKL
60 70 80 90 100
HFKSIAHELL WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS
110 120 130 140 150
WRGADGETID QISRLIEDIK TNPNSRRLIV SAWNVGEIDK MALPPCHCLF
160 170 180 190 200
QFYVSDGKLS CQLYQRSADV FLGVPFNIAS YALLTMIIAH VTGLEPGEFI
210 220 230 240 250
HTFGDVHIYQ NHIEQVNLQL ERDVRPLPQL RFARKVDSIF NFAFEDFIIE
260
DYDPHPHIKG AVSV
Length:264
Mass (Da):30,538
Last modified:July 1, 1989 - v1
Checksum:i1BFE1F1BD8202E64
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti7F → L in strain: ATCC 6633. 1
Natural varianti37N → H in strain: ATCC 6633. 1
Natural varianti39R → Q in strain: ATCC 6633. 1
Natural varianti187I → M in strain: ATCC 6633. 1
Natural varianti221E → T in strain: ATCC 6633. 1
Natural varianti224V → L in strain: ATCC 6633. 1
Natural varianti229Q → K in strain: ATCC 6633. 1
Natural varianti235 – 236KV → EI in strain: ATCC 6633. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69661 Genomic DNA. Translation: CAA49350.1.
L77246 Genomic DNA. Translation: AAA96634.1.
M20012 Genomic DNA. Translation: AAA22852.1.
AL009126 Genomic DNA. Translation: CAB14100.1.
PIRiJT0290. SYBSTB.
S35239.
RefSeqiNP_390065.1. NC_000964.3.
WP_004398587.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14100; CAB14100; BSU21820.
GeneIDi939092.
KEGGibsu:BSU21820.
PATRICi18976169. VBIBacSub10457_2275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69661 Genomic DNA. Translation: CAA49350.1.
L77246 Genomic DNA. Translation: AAA96634.1.
M20012 Genomic DNA. Translation: AAA22852.1.
AL009126 Genomic DNA. Translation: CAB14100.1.
PIRiJT0290. SYBSTB.
S35239.
RefSeqiNP_390065.1. NC_000964.3.
WP_004398587.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP11044.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11044. 6 interactors.
STRINGi224308.Bsubs1_010100012016.

Proteomic databases

PaxDbiP11044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14100; CAB14100; BSU21820.
GeneIDi939092.
KEGGibsu:BSU21820.
PATRICi18976169. VBIBacSub10457_2275.

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
InParanoidiP11044.
KOiK00560.
OMAiIVYELLW.
PhylomeDBiP11044.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciBSUB:BSU21820-MONOMER.
SABIO-RKP11044.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY2_BACSU
AccessioniPrimary (citable) accession number: P11044
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.