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P11044

- TYSY2_BACSU

UniProt

P11044 - TYSY2_BACSU

Protein

Thymidylate synthase 2

Gene

thyA2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Temperature dependencei

    Thermolabile. Inactive at 46 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciBSUB:BSU21820-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase 2UniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TS 2UniRule annotation
    Short name:
    TSase 2UniRule annotation
    Alternative name(s):
    Thymidylate synthase BUniRule annotation
    Short name:
    TS BUniRule annotation
    Short name:
    TSase BUniRule annotation
    Gene namesi
    Name:thyA2UniRule annotation
    Synonyms:thyB
    Ordered Locus Names:BSU21820
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU21820. [Micado]

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthase 2PRO_0000140932Add
    BLAST

    Proteomic databases

    PaxDbiP11044.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP11044. 6 interactions.
    STRINGi224308.BSU21820.

    Structurei

    3D structure databases

    ProteinModelPortaliP11044.
    SMRiP11044. Positions 1-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257899.
    KOiK00560.
    OMAiDVHLYSN.
    OrthoDBiEOG6K6V53.
    PhylomeDBiP11044.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11044-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQYKDFCRH VLEHGEKKGD RTGTGTISTF GYQMRFNLRE GFPMLTTKKL    50
    HFKSIAHELL WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS 100
    WRGADGETID QISRLIEDIK TNPNSRRLIV SAWNVGEIDK MALPPCHCLF 150
    QFYVSDGKLS CQLYQRSADV FLGVPFNIAS YALLTMIIAH VTGLEPGEFI 200
    HTFGDVHIYQ NHIEQVNLQL ERDVRPLPQL RFARKVDSIF NFAFEDFIIE 250
    DYDPHPHIKG AVSV 264
    Length:264
    Mass (Da):30,538
    Last modified:July 1, 1989 - v1
    Checksum:i1BFE1F1BD8202E64
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti7 – 71F → L in strain: ATCC 6633.
    Natural varianti37 – 371N → H in strain: ATCC 6633.
    Natural varianti39 – 391R → Q in strain: ATCC 6633.
    Natural varianti187 – 1871I → M in strain: ATCC 6633.
    Natural varianti221 – 2211E → T in strain: ATCC 6633.
    Natural varianti224 – 2241V → L in strain: ATCC 6633.
    Natural varianti229 – 2291Q → K in strain: ATCC 6633.
    Natural varianti235 – 2362KV → EI in strain: ATCC 6633.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69661 Genomic DNA. Translation: CAA49350.1.
    L77246 Genomic DNA. Translation: AAA96634.1.
    M20012 Genomic DNA. Translation: AAA22852.1.
    AL009126 Genomic DNA. Translation: CAB14100.1.
    PIRiJT0290. SYBSTB.
    S35239.
    RefSeqiNP_390065.1. NC_000964.3.
    WP_004398587.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14100; CAB14100; BSU21820.
    GeneIDi939092.
    KEGGibsu:BSU21820.
    PATRICi18976169. VBIBacSub10457_2275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69661 Genomic DNA. Translation: CAA49350.1 .
    L77246 Genomic DNA. Translation: AAA96634.1 .
    M20012 Genomic DNA. Translation: AAA22852.1 .
    AL009126 Genomic DNA. Translation: CAB14100.1 .
    PIRi JT0290. SYBSTB.
    S35239.
    RefSeqi NP_390065.1. NC_000964.3.
    WP_004398587.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P11044.
    SMRi P11044. Positions 1-250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P11044. 6 interactions.
    STRINGi 224308.BSU21820.

    Proteomic databases

    PaxDbi P11044.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14100 ; CAB14100 ; BSU21820 .
    GeneIDi 939092.
    KEGGi bsu:BSU21820.
    PATRICi 18976169. VBIBacSub10457_2275.

    Organism-specific databases

    GenoListi BSU21820. [Micado ]

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257899.
    KOi K00560.
    OMAi DVHLYSN.
    OrthoDBi EOG6K6V53.
    PhylomeDBi P11044.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci BSUB:BSU21820-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon."
      Iwakura M., Kawata M., Tsuda K., Tanaka T.
      Gene 64:9-20(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SP beta prophage: use of long accurate PCR and yeast artificial chromosomes for sequencing."
      Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S.D.
      Microbiology 142:3005-3015(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    3. "Heat-stable and heat-labile thymidylate synthases B of Bacillus subtilis: comparison of the nucleotide and amino acid sequences."
      Montorsi M., Lorenzetti R.
      Mol. Gen. Genet. 239:1-5(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 and ATCC 6633 / PCI 219 / NRS 231.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiTYSY2_BACSU
    AccessioniPrimary (citable) accession number: P11044
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3