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Protein

Nitrate reductase [NADH] 2

Gene

NIA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi191MolybdenumBy similarity1
Metal bindingi577Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi600Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • nitrate assimilation Source: TAIR
  • nitric oxide biosynthetic process Source: TAIR
  • response to herbicide Source: UniProtKB-KW
  • response to light stimulus Source: TAIR
  • response to symbiotic fungus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Herbicide resistance, Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciMetaCyc:AT1G37130-MONOMER.
BRENDAi1.7.1.1. 399.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-196836. Vitamin C (ascorbate) metabolism.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] 2 (EC:1.7.1.1)
Short name:
NR2
Gene namesi
Name:NIA2
Synonyms:CHL3
Ordered Locus Names:At1g37130
ORF Names:F28L22.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G37130.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660501 – 917Nitrate reductase [NADH] 2Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi433InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP11035.
PRIDEiP11035.

PTM databases

iPTMnetiP11035.

Expressioni

Tissue specificityi

Root, leaf, and shoot.

Gene expression databases

ExpressionAtlasiP11035. baseline and differential.
GenevisibleiP11035. AT.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi25859. 14 interactors.
IntActiP11035. 1 interactor.
STRINGi3702.AT1G37130.1.

Structurei

3D structure databases

ProteinModelPortaliP11035.
SMRiP11035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini542 – 617Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini660 – 772FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.
InParanoidiP11035.
KOiK10534.
OMAiITPIWST.
OrthoDBiEOG0936034C.
PhylomeDBiP11035.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVDNRQY ARLEPGLNGV VRSYKPPVPG RSDSPKAHQN QTTNQTVFLK
60 70 80 90 100
PAKVHDDDED VSSEDENETH NSNAVYYKEM IRKSNAELEP SVLDPRDEYT
110 120 130 140 150
ADSWIERNPS MVRLTGKHPF NSEAPLNRLM HHGFITPVPL HYVRNHGHVP
160 170 180 190 200
KAQWAEWTVE VTGFVKRPMK FTMDQLVSEF AYREFAATLV CAGNRRKEQN
210 220 230 240 250
MVKKSKGFNW GSAGVSTSVW RGVPLCDVLR RCGIFSRKGG ALNVCFEGSE
260 270 280 290 300
DLPGGAGTAG SKYGTSIKKE YAMDPSRDII LAYMQNGEYL TPDHGFPVRI
310 320 330 340 350
IIPGFIGGRM VKWLKRIIVT TKESDNFYHF KDNRVLPSLV DAELADEEGW
360 370 380 390 400
WYKPEYIINE LNINSVITTP CHEEILPINA FTTQRPYTLK GYAYSGGGKK
410 420 430 440 450
VTRVEVTVDG GETWNVCALD HQEKPNKYGK FWCWCFWSLE VEVLDLLSAK
460 470 480 490 500
EIAVRAWDET LNTQPEKMIW NLMGMMNNCW FRVKTNVCKP HKGEIGIVFE
510 520 530 540 550
HPTLPGNESG GWMAKERHLE KSADAPPSLK KSVSTPFMNT TAKMYSMSEV
560 570 580 590 600
KKHNSADSCW IIVHGHIYDC TRFLMDHPGG SDSILINAGT DCTEEFEAIH
610 620 630 640 650
SDKAKKMLED YRIGELITTG YSSDSSSPNN SVHGSSAVFS LLAPIGEATP
660 670 680 690 700
VRNLALVNPR AKVPVQLVEK TSISHDVRKF RFALPVEDMV LGLPVGKHIF
710 720 730 740 750
LCATINDKLC LRAYTPSSTV DVVGYFELVV KIYFGGVHPR FPNGGLMSQY
760 770 780 790 800
LDSLPIGSTL EIKGPLGHVE YLGKGSFTVH GKPKFADKLA MLAGGTGITP
810 820 830 840 850
VYQIIQAILK DPEDETEMYV IYANRTEEDI LLREELDGWA EQYPDRLKVW
860 870 880 890 900
YVVESAKEGW AYSTGFISEA IMREHIPDGL DGSALAMACG PPPMIQFAVQ
910
PNLEKMQYNI KEDFLIF
Length:917
Mass (Da):102,844
Last modified:July 1, 1989 - v1
Checksum:iB8909A318C04C39A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03240 mRNA. Translation: AAA32830.1.
AC007505 Genomic DNA. Translation: AAF19225.1.
CP002684 Genomic DNA. Translation: AEE31891.1.
AF367272 mRNA. Translation: AAK56261.1.
AF436835 mRNA. Translation: AAL32017.1.
AY037183 mRNA. Translation: AAK59768.1.
AY039914 mRNA. Translation: AAK64018.1.
AY133530 mRNA. Translation: AAM91360.1.
AY142568 mRNA. Translation: AAN13137.1.
S45385 Genomic DNA. Translation: AAL32273.1.
X13435 mRNA. Translation: CAA31787.1.
PIRiA31821. RDMUNH.
RefSeqiNP_174901.1. NM_103364.3.
UniGeneiAt.23731.
At.72953.
At.74746.
At.74925.

Genome annotation databases

EnsemblPlantsiAT1G37130.1; AT1G37130.1; AT1G37130.
GeneIDi840630.
GrameneiAT1G37130.1; AT1G37130.1; AT1G37130.
KEGGiath:AT1G37130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03240 mRNA. Translation: AAA32830.1.
AC007505 Genomic DNA. Translation: AAF19225.1.
CP002684 Genomic DNA. Translation: AEE31891.1.
AF367272 mRNA. Translation: AAK56261.1.
AF436835 mRNA. Translation: AAL32017.1.
AY037183 mRNA. Translation: AAK59768.1.
AY039914 mRNA. Translation: AAK64018.1.
AY133530 mRNA. Translation: AAM91360.1.
AY142568 mRNA. Translation: AAN13137.1.
S45385 Genomic DNA. Translation: AAL32273.1.
X13435 mRNA. Translation: CAA31787.1.
PIRiA31821. RDMUNH.
RefSeqiNP_174901.1. NM_103364.3.
UniGeneiAt.23731.
At.72953.
At.74746.
At.74925.

3D structure databases

ProteinModelPortaliP11035.
SMRiP11035.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25859. 14 interactors.
IntActiP11035. 1 interactor.
STRINGi3702.AT1G37130.1.

PTM databases

iPTMnetiP11035.

Proteomic databases

PaxDbiP11035.
PRIDEiP11035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G37130.1; AT1G37130.1; AT1G37130.
GeneIDi840630.
GrameneiAT1G37130.1; AT1G37130.1; AT1G37130.
KEGGiath:AT1G37130.

Organism-specific databases

TAIRiAT1G37130.

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.
InParanoidiP11035.
KOiK10534.
OMAiITPIWST.
OrthoDBiEOG0936034C.
PhylomeDBiP11035.

Enzyme and pathway databases

BioCyciMetaCyc:AT1G37130-MONOMER.
BRENDAi1.7.1.1. 399.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-196836. Vitamin C (ascorbate) metabolism.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP11035.

Gene expression databases

ExpressionAtlasiP11035. baseline and differential.
GenevisibleiP11035. AT.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA2_ARATH
AccessioniPrimary (citable) accession number: P11035
Secondary accession number(s): Q7Y260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

When mutated confers resistance to the herbicide chlorate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.