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Protein

Mast cell protease 1

Gene

Mcpt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a chymotrypsin-like activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei109 – 1091Charge relay systemBy similarity
Active sitei202 – 2021Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  1. protein processing Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.458.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 1 (EC:3.4.21.-)
Short name:
mMCP-1
Gene namesi
Name:Mcpt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96937. Mcpt1.

Subcellular locationi

Secreted. Cytoplasmic granule
Note: Secretory granules.

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. intracellular Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 202Activation peptide2 PublicationsPRO_0000027449
Chaini21 – 246226Mast cell protease 1PRO_0000027450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP11034.
PaxDbiP11034.
PRIDEiP11034.

Expressioni

Tissue specificityi

Mucosal mast cells.

Gene expression databases

BgeeiP11034.
CleanExiMM_MCPT1.
ExpressionAtlasiP11034. baseline and differential.
GenevestigatoriP11034.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022836.

Structurei

3D structure databases

SMRiP11034. Positions 21-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 244224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP11034.
KOiK08661.
OMAiDEEACKI.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP11034.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVEARPH SRPYMAHLKI ITDRGSEDRC
60 70 80 90 100
GGFLIAPQFV LTAAHCKGRE ITVTLGAHDV SKSESTQQRI KVEKQIIHKN
110 120 130 140 150
YNVSFNLYDI MLLKLEEKAE LTPTVDVIPL PGPSDFIDPG KMCWTAGWGK
160 170 180 190 200
TGEKEPTSET LREVELRIMD KEACKMYKHY DYNFQVCVGS STKLKTAYMG
210 220 230 240
DSGGPLLCAG VAHGIVSYGD SHGKPPAVFT RISAYVPWIK TVINGK
Length:246
Mass (Da):27,013
Last modified:November 30, 1992 - v2
Checksum:i26E112E8C580154B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S44609 mRNA. Translation: AAB23194.1.
X68803 Genomic DNA. Translation: CAA48703.1.
CCDSiCCDS27137.1.
PIRiA46504.
RefSeqiNP_032596.1. NM_008570.1.
UniGeneiMm.201549.

Genome annotation databases

EnsembliENSMUST00000022836; ENSMUSP00000022836; ENSMUSG00000022227.
GeneIDi17224.
KEGGimmu:17224.
UCSCiuc007ubi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S44609 mRNA. Translation: AAB23194.1.
X68803 Genomic DNA. Translation: CAA48703.1.
CCDSiCCDS27137.1.
PIRiA46504.
RefSeqiNP_032596.1. NM_008570.1.
UniGeneiMm.201549.

3D structure databases

SMRiP11034. Positions 21-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022836.

Protein family/group databases

MEROPSiS01.458.

Proteomic databases

MaxQBiP11034.
PaxDbiP11034.
PRIDEiP11034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022836; ENSMUSP00000022836; ENSMUSG00000022227.
GeneIDi17224.
KEGGimmu:17224.
UCSCiuc007ubi.1. mouse.

Organism-specific databases

CTDi17224.
MGIiMGI:96937. Mcpt1.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP11034.
KOiK08661.
OMAiDEEACKI.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP11034.
TreeFamiTF333630.

Miscellaneous databases

NextBioi291636.
PROiP11034.
SOURCEiSearch...

Gene expression databases

BgeeiP11034.
CleanExiMM_MCPT1.
ExpressionAtlasiP11034. baseline and differential.
GenevestigatoriP11034.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice."
    Ghildyal N., McNeil H.P., Stechschulte S., Austen K.F., Silberstein D., Gurish M.F., Somerville L.L., Stevens R.L.
    J. Immunol. 149:2123-2129(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases."
    Huang R., Blom T., Hellman L.
    Eur. J. Immunol. 21:1611-1621(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
    Tissue: Liver.
  3. Cited for: PROTEIN SEQUENCE OF 21-246.
  4. "Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4)."
    Newlands G.F.J., Knox D.P., Pirie-Shepherd S.R., Miller H.R.P.
    Biochem. J. 294:127-135(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-49.

Entry informationi

Entry nameiMCPT1_MOUSE
AccessioniPrimary (citable) accession number: P11034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: November 30, 1992
Last modified: March 31, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.