P11034 (MCPT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mast cell protease 1
Short name=mMCP-1
EC=3.4.21.-

Gene names

Name:

Mcpt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a chymotrypsin-like activity.
Subcellular location	Secreted. Cytoplasmic granule. Note: Secretory granules.
Tissue specificity	Mucosal mast cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from direct assay PubMed 9360993. Source: MGI
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Propeptide	19 – 20	2	Activation peptide		PRO_0000027449
	Chain	21 – 246	226	Mast cell protease 1		PRO_0000027450
Regions
	Domain	21 – 244	224	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Active site	202	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	143 ↔ 208		By similarity
	Disulfide bond	174 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11034 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: 26E112E8C580154B	FASTA	246	27,013
	10 20 30 40 50 60 MQALLFLMAL LLPSGAGAEE IIGGVEARPH SRPYMAHLKI ITDRGSEDRC GGFLIAPQFV 70 80 90 100 110 120 LTAAHCKGRE ITVTLGAHDV SKSESTQQRI KVEKQIIHKN YNVSFNLYDI MLLKLEEKAE 130 140 150 160 170 180 LTPTVDVIPL PGPSDFIDPG KMCWTAGWGK TGEKEPTSET LREVELRIMD KEACKMYKHY 190 200 210 220 230 240 DYNFQVCVGS STKLKTAYMG DSGGPLLCAG VAHGIVSYGD SHGKPPAVFT RISAYVPWIK TVINGK

References

[1]	"IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice." Ghildyal N., McNeil H.P., Stechschulte S., Austen K.F., Silberstein D., Gurish M.F., Somerville L.L., Stevens R.L. J. Immunol. 149:2123-2129(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases." Huang R., Blom T., Hellman L. Eur. J. Immunol. 21:1611-1621(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: BALB/c. Tissue: Liver.
[3]	"Amino acid sequence of a mouse mucosal mast cell protease." le Trong H., Newlands G.F.J., Miller H.R.P., Charbonneau H., Neurath H., Woodbury R.G. Biochemistry 28:391-395(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-246.
[4]	"Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4)." Newlands G.F.J., Knox D.P., Pirie-Shepherd S.R., Miller H.R.P. Biochem. J. 294:127-135(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-49.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S44609 mRNA. Translation: AAB23194.1. X68803 Genomic DNA. Translation: CAA48703.1.
IPI	IPI00111387.
PIR	A46504.
RefSeq	NP_032596.1. NM_008570.1.
UniGene	Mm.201549.
3D structure databases
ProteinModelPortal	P11034.
SMR	P11034. Positions 21-244.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000022836.
Protein family/group databases
MEROPS	S01.458.
Proteomic databases
PaxDb	P11034.
PRIDE	P11034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022836; ENSMUSP00000022836; ENSMUSG00000022227.
GeneID	17224.
KEGG	mmu:17224.
UCSC	uc007ubi.1. mouse.
Organism-specific databases
CTD	17224.
MGI	MGI:96937. Mcpt1.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P11034.
KO	K08661.
OMA	CKGREIT.
OrthoDB	EOG47WNQN.
Gene expression databases
ArrayExpress	P11034.
Bgee	P11034.
CleanEx	MM_MCPT1.
Genevestigator	P11034.
GermOnline	ENSMUSG00000022227. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291636.
SOURCE	Search...

Entry information

Entry name

MCPT1_MOUSE

Accession

Primary (citable) accession number: P11034

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 1, 1992
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program