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P11032

- GRAA_MOUSE

UniProt

P11032 - GRAA_MOUSE

Protein

Granzyme A

Gene

Gzma

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 2 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA By similarity.By similarity

    Catalytic activityi

    Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691Charge relay systemBy similarity
    Active sitei113 – 1131Charge relay systemBy similarity
    Active sitei211 – 2111Charge relay systemBy similarity

    GO - Molecular functioni

    1. protein homodimerization activity Source: UniProtKB
    2. serine-type endopeptidase activity Source: UniProtKB
    3. serine-type peptidase activity Source: MGI

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cytolysis Source: UniProtKB-KW
    3. negative regulation of DNA binding Source: UniProtKB
    4. negative regulation of endodeoxyribonuclease activity Source: UniProtKB
    5. negative regulation of oxidoreductase activity Source: UniProtKB
    6. positive regulation of apoptotic process Source: UniProtKB
    7. proteolysis involved in cellular protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Apoptosis, Cytolysis

    Enzyme and pathway databases

    BRENDAi3.4.21.78. 3474.

    Protein family/group databases

    MEROPSiS01.135.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granzyme A (EC:3.4.21.78)
    Alternative name(s):
    Autocrine thymic lymphoma granzyme-like serine protease
    CTLA-3
    Fragmentin-1
    T cell-specific serine protease 1
    Short name:
    TSP-1
    Gene namesi
    Name:Gzma
    Synonyms:Ctla-3, Ctla3, Mtsp-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:109266. Gzma.

    Subcellular locationi

    Secreted. Cytoplasmic granule
    Note: Cytoplasmic granules of cytolytic T-lymphocytes.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Add
    BLAST
    Propeptidei27 – 282Activation peptide2 PublicationsPRO_0000027395
    Chaini29 – 260232Granzyme APRO_0000027396Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
    Disulfide bondi147 ↔ 217PROSITE-ProRule annotation
    Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi178 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP11032.
    PaxDbiP11032.
    PRIDEiP11032.

    PTM databases

    PhosphoSiteiP11032.

    Expressioni

    Tissue specificityi

    Found in cytotoxic lymphocytes and in normal lymphoid tissues such as thymus and spleen.

    Gene expression databases

    ArrayExpressiP11032.
    BgeeiP11032.
    CleanExiMM_GZMA.
    GenevestigatoriP11032.

    Interactioni

    Subunit structurei

    Interacts with APEX1 By similarity. Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    IntActiP11032. 1 interaction.
    MINTiMINT-4096608.

    Structurei

    3D structure databases

    ProteinModelPortaliP11032.
    SMRiP11032. Positions 29-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 257229Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP11032.
    KOiK01352.
    OMAiSINKEPE.
    OrthoDBiEOG7W9RVH.
    PhylomeDBiP11032.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform HF1 (identifier: P11032-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRNASGPRGP SLATLLFLLL IPEGGCERII GGDTVVPHSR PYMALLKLSS    50
    NTICAGALIE KNWVLTAAHC NVGKRSKFIL GAHSINKEPE QQILTVKKAF 100
    PYPCYDEYTR EGDLQLVRLK KKATVNRNVA ILHLPKKGDD VKPGTRCRVA 150
    GWGRFGNKSA PSETLREVNI TVIDRKICND EKHYNFHPVI GLNMICAGDL 200
    RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV YTFLSDKHLN 250
    WIKKIMKGSV 260

    Note: More abundant in lymphoid tissues than isoform HF2.

    Length:260
    Mass (Da):28,599
    Last modified:July 1, 1993 - v2
    Checksum:i7705352F08DEED8D
    GO
    Isoform HF2 (identifier: P11032-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-23: MRNASGPRGPSLATLLFLLLIPE → MSKEMNEILLSWEINLSSKR

    Show »
    Length:257
    Mass (Da):28,541
    Checksum:i7D67F97E532F7440
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2323MRNAS…LLIPE → MSKEMNEILLSWEINLSSKR in isoform HF2. CuratedVSP_005373Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14799 mRNA. Translation: CAA32905.1.
    X62542
    , X62543, X60310, X60311 Genomic DNA. Translation: CAA44426.1.
    L01429
    , L01426, L01427, L01441 Genomic DNA. Translation: AAA99898.1.
    L01429
    , L01426, L01427, L01441 Genomic DNA. Translation: AAA99897.1.
    BC061146 mRNA. Translation: AAH61146.1.
    M26183 mRNA. Translation: AAA37735.1.
    M13226 mRNA. Translation: AAA40134.1.
    CCDSiCCDS26782.1. [P11032-1]
    PIRiA24807.
    A45061.
    B45061.
    RefSeqiNP_034500.1. NM_010370.2. [P11032-1]
    UniGeneiMm.15510.

    Genome annotation databases

    EnsembliENSMUST00000023897; ENSMUSP00000023897; ENSMUSG00000023132. [P11032-1]
    GeneIDi14938.
    KEGGimmu:14938.
    UCSCiuc007rxc.1. mouse. [P11032-1]
    uc011zeu.1. mouse. [P11032-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14799 mRNA. Translation: CAA32905.1 .
    X62542
    , X62543 , X60310 , X60311 Genomic DNA. Translation: CAA44426.1 .
    L01429
    , L01426 , L01427 , L01441 Genomic DNA. Translation: AAA99898.1 .
    L01429
    , L01426 , L01427 , L01441 Genomic DNA. Translation: AAA99897.1 .
    BC061146 mRNA. Translation: AAH61146.1 .
    M26183 mRNA. Translation: AAA37735.1 .
    M13226 mRNA. Translation: AAA40134.1 .
    CCDSi CCDS26782.1. [P11032-1 ]
    PIRi A24807.
    A45061.
    B45061.
    RefSeqi NP_034500.1. NM_010370.2. [P11032-1 ]
    UniGenei Mm.15510.

    3D structure databases

    ProteinModelPortali P11032.
    SMRi P11032. Positions 29-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P11032. 1 interaction.
    MINTi MINT-4096608.

    Protein family/group databases

    MEROPSi S01.135.

    PTM databases

    PhosphoSitei P11032.

    Proteomic databases

    MaxQBi P11032.
    PaxDbi P11032.
    PRIDEi P11032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023897 ; ENSMUSP00000023897 ; ENSMUSG00000023132 . [P11032-1 ]
    GeneIDi 14938.
    KEGGi mmu:14938.
    UCSCi uc007rxc.1. mouse. [P11032-1 ]
    uc011zeu.1. mouse. [P11032-2 ]

    Organism-specific databases

    CTDi 3001.
    MGIi MGI:109266. Gzma.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P11032.
    KOi K01352.
    OMAi SINKEPE.
    OrthoDBi EOG7W9RVH.
    PhylomeDBi P11032.
    TreeFami TF333630.

    Enzyme and pathway databases

    BRENDAi 3.4.21.78. 3474.

    Miscellaneous databases

    NextBioi 287259.
    PROi P11032.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P11032.
    Bgeei P11032.
    CleanExi MM_GZMA.
    Genevestigatori P11032.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA clones from autocrine thymic lymphoma cells encode two mitogenic proteins, a serine protease and a truncated T-cell receptor beta-chain."
      Bogenberger J., Haas M.
      Oncogene Res. 3:301-312(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Organization of the gene encoding the mouse T-cell-specific serine proteinase 'granzyme A'."
      Ebnet K., Kramer M.D., Simon M.M.
      Genomics 13:502-508(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6.
    3. "Genomic organization of the mouse granzyme A gene. Two mRNAs encode the same mature granzyme A with different leader peptides."
      Hershberger R.J., Gershenfeld H.K., Weissman I.L., Su L.
      J. Biol. Chem. 267:25488-25493(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS HF1 AND HF2).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HF1).
    5. "Granzymes, a family of serine proteases released from granules of cytolytic T lymphocytes upon T cell receptor stimulation."
      Jenne D.E., Tschopp J.
      Immunol. Rev. 103:53-71(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
    6. "Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte."
      Gershenfeld H.K., Weissman I.L.
      Science 232:854-858(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-260.
    7. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
      Masson D., Tschopp J.
      Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-48.
    8. "Identification of granzyme A isolated from cytotoxic T-lymphocyte-granules as one of the proteases encoded by CTL-specific genes."
      Masson D., Zamai M., Tschopp J.
      FEBS Lett. 208:84-88(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-53.
    9. "Induction of T cell serine proteinase 1 (TSP-1)-specific mRNA in mouse T lymphocytes."
      Simon H.G., Fruth U., Eckerskorn C., Lottspeich F., Kramer M.D., Nerz G., Simon M.M.
      Eur. J. Immunol. 18:855-861(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-46.

    Entry informationi

    Entry nameiGRAA_MOUSE
    AccessioniPrimary (citable) accession number: P11032
    Secondary accession number(s): P15118
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 148 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3