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P11032 (GRAA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granzyme A

EC=3.4.21.78
Alternative name(s):
Autocrine thymic lymphoma granzyme-like serine protease
CTLA-3
Fragmentin-1
T cell-specific serine protease 1
Short name=TSP-1
Gene names
Name:Gzma
Synonyms:Ctla-3, Ctla3, Mtsp-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-30' and destroys its oxidative repair activity. Involved in apoptosis By similarity.

Catalytic activity

Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates.

Subunit structure

Interacts with APEX1 By similarity. Homodimer; disulfide-linked.

Subcellular location

Secreted. Cytoplasmic granule. Note: Cytoplasmic granules of cytolytic T-lymphocytes.

Tissue specificity

Found in cytotoxic lymphocytes and in normal lymphoid tissues such as thymus and spleen.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Caution

The predicted cleavage site for the activation peptide of HF2 is uncertain. It could have either 2 (ER) or 7 (KRGGCER) AA.

Ontologies

Keywords
   Biological processApoptosis
Cytolysis
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

cytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of endodeoxyribonuclease activity

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of oxidoreductase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

proteolysis involved in cellular protein catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 1860869. Source: UniProtKB

   Molecular_functionprotein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

serine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

serine-type peptidase activity

Inferred from direct assay PubMed 16618603. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform HF1 (identifier: P11032-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: More abundant in lymphoid tissues than isoform HF2.
Isoform HF2 (identifier: P11032-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MRNASGPRGPSLATLLFLLLIPE → MSKEMNEILLSWEINLSSKR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Propeptide27 – 282Activation peptide
PRO_0000027395
Chain29 – 260232Granzyme A
PRO_0000027396

Regions

Domain29 – 257229Peptidase S1

Sites

Active site691Charge relay system By similarity
Active site1131Charge relay system By similarity
Active site2111Charge relay system By similarity

Amino acid modifications

Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 70 By similarity
Disulfide bond147 ↔ 217 By similarity
Disulfide bond178 ↔ 196 By similarity
Disulfide bond207 ↔ 232 By similarity

Natural variations

Alternative sequence1 – 2323MRNAS…LLIPE → MSKEMNEILLSWEINLSSKR in isoform HF2.
VSP_005373

Sequences

Sequence LengthMass (Da)Tools
Isoform HF1 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 7705352F08DEED8D

FASTA26028,599
        10         20         30         40         50         60 
MRNASGPRGP SLATLLFLLL IPEGGCERII GGDTVVPHSR PYMALLKLSS NTICAGALIE 

        70         80         90        100        110        120 
KNWVLTAAHC NVGKRSKFIL GAHSINKEPE QQILTVKKAF PYPCYDEYTR EGDLQLVRLK 

       130        140        150        160        170        180 
KKATVNRNVA ILHLPKKGDD VKPGTRCRVA GWGRFGNKSA PSETLREVNI TVIDRKICND 

       190        200        210        220        230        240 
EKHYNFHPVI GLNMICAGDL RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV 

       250        260 
YTFLSDKHLN WIKKIMKGSV 

« Hide

Isoform HF2 [UniParc].

Checksum: 7D67F97E532F7440
Show »

FASTA25728,541

References

« Hide 'large scale' references
[1]"cDNA clones from autocrine thymic lymphoma cells encode two mitogenic proteins, a serine protease and a truncated T-cell receptor beta-chain."
Bogenberger J., Haas M.
Oncogene Res. 3:301-312(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"Organization of the gene encoding the mouse T-cell-specific serine proteinase 'granzyme A'."
Ebnet K., Kramer M.D., Simon M.M.
Genomics 13:502-508(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6.
[3]"Genomic organization of the mouse granzyme A gene. Two mRNAs encode the same mature granzyme A with different leader peptides."
Hershberger R.J., Gershenfeld H.K., Weissman I.L., Su L.
J. Biol. Chem. 267:25488-25493(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS HF1 AND HF2).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HF1).
[5]"Granzymes, a family of serine proteases released from granules of cytolytic T lymphocytes upon T cell receptor stimulation."
Jenne D.E., Tschopp J.
Immunol. Rev. 103:53-71(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
[6]"Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte."
Gershenfeld H.K., Weissman I.L.
Science 232:854-858(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-260.
[7]"A family of serine esterases in lytic granules of cytolytic T lymphocytes."
Masson D., Tschopp J.
Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-48.
[8]"Identification of granzyme A isolated from cytotoxic T-lymphocyte-granules as one of the proteases encoded by CTL-specific genes."
Masson D., Zamai M., Tschopp J.
FEBS Lett. 208:84-88(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-53.
[9]"Induction of T cell serine proteinase 1 (TSP-1)-specific mRNA in mouse T lymphocytes."
Simon H.G., Fruth U., Eckerskorn C., Lottspeich F., Kramer M.D., Nerz G., Simon M.M.
Eur. J. Immunol. 18:855-861(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-46.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14799 mRNA. Translation: CAA32905.1.
X62542 expand/collapse EMBL AC list , X62543, X60310, X60311 Genomic DNA. Translation: CAA44426.1.
L01429 expand/collapse EMBL AC list , L01426, L01427, L01441 Genomic DNA. Translation: AAA99898.1.
L01429 expand/collapse EMBL AC list , L01426, L01427, L01441 Genomic DNA. Translation: AAA99897.1.
BC061146 mRNA. Translation: AAH61146.1.
M26183 mRNA. Translation: AAA37735.1.
M13226 mRNA. Translation: AAA40134.1.
PIRA24807.
A45061.
B45061.
RefSeqNP_034500.1. NM_010370.2.
UniGeneMm.15510.

3D structure databases

ProteinModelPortalP11032.
SMRP11032. Positions 29-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP11032. 1 interaction.
MINTMINT-4096608.

Protein family/group databases

MEROPSS01.135.

PTM databases

PhosphoSiteP11032.

Proteomic databases

PaxDbP11032.
PRIDEP11032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023897; ENSMUSP00000023897; ENSMUSG00000023132. [P11032-1]
GeneID14938.
KEGGmmu:14938.
UCSCuc007rxc.1. mouse. [P11032-1]
uc011zeu.1. mouse. [P11032-2]

Organism-specific databases

CTD3001.
MGIMGI:109266. Gzma.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP11032.
KOK01352.
OMASINKEPE.
OrthoDBEOG7W9RVH.
PhylomeDBP11032.
TreeFamTF333630.

Enzyme and pathway databases

BRENDA3.4.21.78. 3474.

Gene expression databases

ArrayExpressP11032.
BgeeP11032.
CleanExMM_GZMA.
GenevestigatorP11032.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287259.
PROP11032.
SOURCESearch...

Entry information

Entry nameGRAA_MOUSE
AccessionPrimary (citable) accession number: P11032
Secondary accession number(s): P15118
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot