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Protein

Activated RNA polymerase II transcriptional coactivator p15

Gene

Sub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Activated RNA polymerase II transcriptional coactivator p15
Alternative name(s):
Positive cofactor 4
Short name:
PC4
SUB1 homolog
Single-stranded DNA-binding protein p9
p14
Gene namesi
Name:Sub1
Synonyms:Pc4, Rpo2tc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:104811 Sub1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000232861 – 127Activated RNA polymerase II transcriptional coactivator p15Add BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei17PhosphoserineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei35N6-acetyllysineBy similarity1
Modified residuei53N6-acetyllysineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Modified residuei56PhosphoserineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei58PhosphoserineBy similarity1
Modified residuei68N6-acetyllysine; alternateBy similarity1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei118PhosphoserineBy similarity1

Post-translational modificationi

Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei50 – 51Cleavage2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP11031
MaxQBiP11031
PaxDbiP11031
PeptideAtlasiP11031
PRIDEiP11031

PTM databases

iPTMnetiP11031
PhosphoSitePlusiP11031

Expressioni

Gene expression databases

BgeeiENSMUSG00000022205
CleanExiMM_SUB1
GenevisibleiP11031 MM

Interactioni

Subunit structurei

Homodimer. Interacts with CSTF2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202999, 3 interactors
CORUMiP11031
IntActiP11031, 3 interactors
MINTiP11031
STRINGi10090.ENSMUSP00000022816

Structurei

3D structure databases

ProteinModelPortaliP11031
SMRiP11031
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 50RegulatoryAdd BLAST50
Regioni77 – 101Interaction with ssDNABy similarityAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 19Ser-richAdd BLAST16
Compositional biasi23 – 53Lys-richAdd BLAST31
Compositional biasi51 – 58Ser-rich8

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2712 Eukaryota
ENOG410XUB8 LUCA
GeneTreeiENSGT00390000008802
HOGENOMiHOG000239157
HOVERGENiHBG028243
InParanoidiP11031
OMAiMVDIREH
OrthoDBiEOG091G14VO
PhylomeDBiP11031
TreeFamiTF313859

Family and domain databases

Gene3Di2.30.31.10, 1 hit
InterProiView protein in InterPro
IPR003173 PC4
IPR009044 ssDNA-bd_transcriptional_reg
PfamiView protein in Pfam
PF02229 PC4, 1 hit
SUPFAMiSSF54447 SSF54447, 1 hit

Sequencei

Sequence statusi: Complete.

P11031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKSKELVSS SSSGSDSDSE VEKKLKRKKQ AVPEKPVKKQ KPGETSRALA
60 70 80 90 100
SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK ILIDIREYWM DSEGEMKPGR
110 120
KGISLNMEQW SQLKEQISDI DDAVRKL
Length:127
Mass (Da):14,427
Last modified:January 23, 2007 - v3
Checksum:i12D58716F40FEB1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46S → P in BAE27090 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03750 mRNA Translation: AAA37317.1
AK048371 mRNA Translation: BAC33314.1
AK049260 mRNA Translation: BAC33642.1
AK132021 mRNA Translation: BAE20946.1
AK146335 mRNA Translation: BAE27090.1
AK151734 mRNA Translation: BAE30649.1
AK154114 mRNA Translation: BAE32384.1
BC010967 mRNA Translation: AAH10967.1
CCDSiCCDS27387.1
PIRiA28084
RefSeqiNP_035424.1, NM_011294.3
XP_006520105.1, XM_006520042.3
UniGeneiMm.41746

Genome annotation databases

EnsembliENSMUST00000022816; ENSMUSP00000022816; ENSMUSG00000022205
ENSMUST00000110504; ENSMUSP00000106130; ENSMUSG00000022205
GeneIDi20024
KEGGimmu:20024
UCSCiuc007vhj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTCP4_MOUSE
AccessioniPrimary (citable) accession number: P11031
Secondary accession number(s): Q3UJR5, Q543N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health