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P11031

- TCP4_MOUSE

UniProt

P11031 - TCP4_MOUSE

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Protein

Activated RNA polymerase II transcriptional coactivator p15

Gene

Sub1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei50 – 512Cleavage

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  3. single-stranded DNA binding Source: Ensembl
  4. transcription coactivator activity Source: Ensembl

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: GOC
  2. SMAD protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Activated RNA polymerase II transcriptional coactivator p15
Alternative name(s):
Positive cofactor 4
Short name:
PC4
SUB1 homolog
Single-stranded DNA-binding protein p9
p14
Gene namesi
Name:Sub1
Synonyms:Pc4, Rpo2tc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:104811. Sub1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. nucleolus Source: Ensembl
  3. transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 127126Activated RNA polymerase II transcriptional coactivator p15PRO_0000023286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei35 – 351N6-acetyllysineBy similarity
Modified residuei53 – 531N6-acetyllysine1 Publication
Modified residuei68 – 681N6-acetyllysineBy similarity
Modified residuei118 – 1181PhosphoserineBy similarity

Post-translational modificationi

Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP11031.
PaxDbiP11031.
PRIDEiP11031.

PTM databases

PhosphoSiteiP11031.

Expressioni

Gene expression databases

BgeeiP11031.
CleanExiMM_SUB1.
GenevestigatoriP11031.

Interactioni

Subunit structurei

Homodimer. Interacts with CSTF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202999. 2 interactions.
IntActiP11031. 2 interactions.
MINTiMINT-4137265.

Structurei

3D structure databases

ProteinModelPortaliP11031.
SMRiP11031. Positions 63-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 5049RegulatoryAdd
BLAST
Regioni77 – 10125Interaction with ssDNABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 1916Ser-richAdd
BLAST
Compositional biasi23 – 5331Lys-richAdd
BLAST
Compositional biasi51 – 588Ser-rich

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG299238.
GeneTreeiENSGT00390000008802.
HOGENOMiHOG000239157.
HOVERGENiHBG028243.
InParanoidiP11031.
OMAiREYWMNQ.
OrthoDBiEOG76MKBV.
PhylomeDBiP11031.
TreeFamiTF313859.

Family and domain databases

Gene3Di2.30.31.10. 1 hit.
InterProiIPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view]
PfamiPF02229. PC4. 1 hit.
[Graphical view]
SUPFAMiSSF54447. SSF54447. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11031-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPKSKELVSS SSSGSDSDSE VEKKLKRKKQ AVPEKPVKKQ KPGETSRALA
60 70 80 90 100
SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK ILIDIREYWM DSEGEMKPGR
110 120
KGISLNMEQW SQLKEQISDI DDAVRKL
Length:127
Mass (Da):14,427
Last modified:January 23, 2007 - v3
Checksum:i12D58716F40FEB1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461S → P in BAE27090. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03750 mRNA. Translation: AAA37317.1.
AK048371 mRNA. Translation: BAC33314.1.
AK049260 mRNA. Translation: BAC33642.1.
AK132021 mRNA. Translation: BAE20946.1.
AK146335 mRNA. Translation: BAE27090.1.
AK151734 mRNA. Translation: BAE30649.1.
AK154114 mRNA. Translation: BAE32384.1.
BC010967 mRNA. Translation: AAH10967.1.
CCDSiCCDS27387.1.
PIRiA28084.
RefSeqiNP_035424.1. NM_011294.3.
XP_006520105.1. XM_006520042.1.
UniGeneiMm.41746.

Genome annotation databases

EnsembliENSMUST00000022816; ENSMUSP00000022816; ENSMUSG00000022205.
ENSMUST00000110504; ENSMUSP00000106130; ENSMUSG00000022205.
GeneIDi20024.
KEGGimmu:20024.
UCSCiuc007vhj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03750 mRNA. Translation: AAA37317.1 .
AK048371 mRNA. Translation: BAC33314.1 .
AK049260 mRNA. Translation: BAC33642.1 .
AK132021 mRNA. Translation: BAE20946.1 .
AK146335 mRNA. Translation: BAE27090.1 .
AK151734 mRNA. Translation: BAE30649.1 .
AK154114 mRNA. Translation: BAE32384.1 .
BC010967 mRNA. Translation: AAH10967.1 .
CCDSi CCDS27387.1.
PIRi A28084.
RefSeqi NP_035424.1. NM_011294.3.
XP_006520105.1. XM_006520042.1.
UniGenei Mm.41746.

3D structure databases

ProteinModelPortali P11031.
SMRi P11031. Positions 63-127.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202999. 2 interactions.
IntActi P11031. 2 interactions.
MINTi MINT-4137265.

PTM databases

PhosphoSitei P11031.

Proteomic databases

MaxQBi P11031.
PaxDbi P11031.
PRIDEi P11031.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022816 ; ENSMUSP00000022816 ; ENSMUSG00000022205 .
ENSMUST00000110504 ; ENSMUSP00000106130 ; ENSMUSG00000022205 .
GeneIDi 20024.
KEGGi mmu:20024.
UCSCi uc007vhj.1. mouse.

Organism-specific databases

CTDi 10923.
MGIi MGI:104811. Sub1.

Phylogenomic databases

eggNOGi NOG299238.
GeneTreei ENSGT00390000008802.
HOGENOMi HOG000239157.
HOVERGENi HBG028243.
InParanoidi P11031.
OMAi REYWMNQ.
OrthoDBi EOG76MKBV.
PhylomeDBi P11031.
TreeFami TF313859.

Miscellaneous databases

ChiTaRSi SUB1. mouse.
NextBioi 297547.
PROi P11031.
SOURCEi Search...

Gene expression databases

Bgeei P11031.
CleanExi MM_SUB1.
Genevestigatori P11031.

Family and domain databases

Gene3Di 2.30.31.10. 1 hit.
InterProi IPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view ]
Pfami PF02229. PC4. 1 hit.
[Graphical view ]
SUPFAMi SSF54447. SSF54447. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel 9-kDa polypeptide from nuclear extracts. DNA binding properties, primary structure, and in vitro expression."
    Ballard D.W., Philbrick W.M., Bothwell A.L.M.
    J. Biol. Chem. 263:8450-8457(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-81 AND 108-127, PROTEOLYTIC PROCESSING, DNA-BINDING.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J, DBA/2 and NOD.
    Tissue: Bone marrow.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiTCP4_MOUSE
AccessioniPrimary (citable) accession number: P11031
Secondary accession number(s): Q3UJR5, Q543N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3