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Protein

Activated RNA polymerase II transcriptional coactivator p15

Gene

Sub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Activated RNA polymerase II transcriptional coactivator p15
Alternative name(s):
Positive cofactor 4
Short name:
PC4
SUB1 homolog
Single-stranded DNA-binding protein p9
p14
Gene namesi
Name:Sub1
Synonyms:Pc4, Rpo2tc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:104811. Sub1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 127126Activated RNA polymerase II transcriptional coactivator p15PRO_0000023286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei9 – 91PhosphoserineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei35 – 351N6-acetyllysineBy similarity
Modified residuei53 – 531N6-acetyllysineCombined sources
Modified residuei55 – 551PhosphoserineCombined sources
Modified residuei56 – 561PhosphoserineBy similarity
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei58 – 581PhosphoserineBy similarity
Modified residuei68 – 681N6-acetyllysine; alternateBy similarity
Cross-linki68 – 68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei118 – 1181PhosphoserineBy similarity

Post-translational modificationi

Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei50 – 512Cleavage

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP11031.
MaxQBiP11031.
PaxDbiP11031.
PeptideAtlasiP11031.
PRIDEiP11031.

PTM databases

iPTMnetiP11031.
PhosphoSiteiP11031.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022205.
CleanExiMM_SUB1.
GenevisibleiP11031. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CSTF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202999. 3 interactions.
IntActiP11031. 3 interactions.
MINTiMINT-4137265.
STRINGi10090.ENSMUSP00000022816.

Structurei

3D structure databases

ProteinModelPortaliP11031.
SMRiP11031. Positions 63-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 5049RegulatoryAdd
BLAST
Regioni77 – 10125Interaction with ssDNABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 1916Ser-richAdd
BLAST
Compositional biasi23 – 5331Lys-richAdd
BLAST
Compositional biasi51 – 588Ser-rich

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2712. Eukaryota.
ENOG410XUB8. LUCA.
GeneTreeiENSGT00390000008802.
HOGENOMiHOG000239157.
HOVERGENiHBG028243.
InParanoidiP11031.
OMAiQWNQLKD.
OrthoDBiEOG091G14VO.
PhylomeDBiP11031.
TreeFamiTF313859.

Family and domain databases

Gene3Di2.30.31.10. 1 hit.
InterProiIPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view]
PfamiPF02229. PC4. 1 hit.
[Graphical view]
SUPFAMiSSF54447. SSF54447. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKSKELVSS SSSGSDSDSE VEKKLKRKKQ AVPEKPVKKQ KPGETSRALA
60 70 80 90 100
SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK ILIDIREYWM DSEGEMKPGR
110 120
KGISLNMEQW SQLKEQISDI DDAVRKL
Length:127
Mass (Da):14,427
Last modified:January 23, 2007 - v3
Checksum:i12D58716F40FEB1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461S → P in BAE27090 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03750 mRNA. Translation: AAA37317.1.
AK048371 mRNA. Translation: BAC33314.1.
AK049260 mRNA. Translation: BAC33642.1.
AK132021 mRNA. Translation: BAE20946.1.
AK146335 mRNA. Translation: BAE27090.1.
AK151734 mRNA. Translation: BAE30649.1.
AK154114 mRNA. Translation: BAE32384.1.
BC010967 mRNA. Translation: AAH10967.1.
CCDSiCCDS27387.1.
PIRiA28084.
RefSeqiNP_035424.1. NM_011294.3.
XP_006520105.1. XM_006520042.2.
UniGeneiMm.41746.

Genome annotation databases

EnsembliENSMUST00000022816; ENSMUSP00000022816; ENSMUSG00000022205.
ENSMUST00000110504; ENSMUSP00000106130; ENSMUSG00000022205.
GeneIDi20024.
KEGGimmu:20024.
UCSCiuc007vhj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03750 mRNA. Translation: AAA37317.1.
AK048371 mRNA. Translation: BAC33314.1.
AK049260 mRNA. Translation: BAC33642.1.
AK132021 mRNA. Translation: BAE20946.1.
AK146335 mRNA. Translation: BAE27090.1.
AK151734 mRNA. Translation: BAE30649.1.
AK154114 mRNA. Translation: BAE32384.1.
BC010967 mRNA. Translation: AAH10967.1.
CCDSiCCDS27387.1.
PIRiA28084.
RefSeqiNP_035424.1. NM_011294.3.
XP_006520105.1. XM_006520042.2.
UniGeneiMm.41746.

3D structure databases

ProteinModelPortaliP11031.
SMRiP11031. Positions 63-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202999. 3 interactions.
IntActiP11031. 3 interactions.
MINTiMINT-4137265.
STRINGi10090.ENSMUSP00000022816.

PTM databases

iPTMnetiP11031.
PhosphoSiteiP11031.

Proteomic databases

EPDiP11031.
MaxQBiP11031.
PaxDbiP11031.
PeptideAtlasiP11031.
PRIDEiP11031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022816; ENSMUSP00000022816; ENSMUSG00000022205.
ENSMUST00000110504; ENSMUSP00000106130; ENSMUSG00000022205.
GeneIDi20024.
KEGGimmu:20024.
UCSCiuc007vhj.1. mouse.

Organism-specific databases

CTDi10923.
MGIiMGI:104811. Sub1.

Phylogenomic databases

eggNOGiKOG2712. Eukaryota.
ENOG410XUB8. LUCA.
GeneTreeiENSGT00390000008802.
HOGENOMiHOG000239157.
HOVERGENiHBG028243.
InParanoidiP11031.
OMAiQWNQLKD.
OrthoDBiEOG091G14VO.
PhylomeDBiP11031.
TreeFamiTF313859.

Miscellaneous databases

ChiTaRSiSub1. mouse.
PROiP11031.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022205.
CleanExiMM_SUB1.
GenevisibleiP11031. MM.

Family and domain databases

Gene3Di2.30.31.10. 1 hit.
InterProiIPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view]
PfamiPF02229. PC4. 1 hit.
[Graphical view]
SUPFAMiSSF54447. SSF54447. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTCP4_MOUSE
AccessioniPrimary (citable) accession number: P11031
Secondary accession number(s): Q3UJR5, Q543N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.