P11030 (ACBP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-CoA-binding protein
Short name=ACBP

Alternative name(s):
Diazepam-binding inhibitor
Short name=DBI
Endozepine
Short name=EP

Cleaved into the following 2 chains:

1. Triakontatetraneuropeptide
   Short name=TTN
2. Octadecaneuropeptide
   Short name=ODN

Gene names

Name:

Dbi

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Subunit structure	Monomer.
Subcellular location	Endoplasmic reticulum By similarity. Golgi apparatus By similarity. Note: Golgi localization is dependent on ligand binding By similarity.
Sequence similarities	Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
Ligand	Lipid-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acyl-CoA metabolic process Traceable author statement Ref.6. Source: RGD brain development Inferred from expression pattern PubMed 10048463. Source: RGD cellular response to nutrient Inferred from expression pattern PubMed 15825833. Source: RGD glial cell proliferation Inferred from direct assay PubMed 10899945. Source: RGD negative regulation of fatty acid beta-oxidation Inferred from direct assay PubMed 11485163. Source: RGD pancreatic juice secretion Inferred from expression pattern PubMed 10675361. Source: RGD positive regulation of lipid biosynthetic process Inferred from direct assay PubMed 11485163. Source: RGD positive regulation of release of sequestered calcium ion into cytosol Inferred from mutant phenotype PubMed 9804683. Source: RGD regulation of synaptic transmission, GABAergic Inferred from direct assay PubMed 3456171. Source: MGI steroid biosynthetic process Traceable author statement Ref.6. Source: RGD
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell contractile fiber Inferred from direct assay PubMed 11812754. Source: RGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 3819722. Source: MGI nucleus Inferred from direct assay PubMed 18455725. Source: RGD plasma membrane Inferred from direct assay PubMed 18455725. Source: RGD synaptic vesicle Inferred from direct assay PubMed 3819722. Source: MGI
Molecular_function	benzodiazepine receptor binding Inferred from direct assay PubMed 6304714. Source: RGD fatty-acyl-CoA binding Inferred from physical interaction PubMed 11404184. Source: RGD lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 87	86	Acyl-CoA-binding protein		PRO_0000045273
	Peptide	18 – 51	34	Triakontatetraneuropeptide Ref.8		PRO_0000000288
	Peptide	34 – 51	18	Octadecaneuropeptide Ref.9		PRO_0000000289
Regions
	Domain	2 – 87	86	ACB
	Region	29 – 33	5	Acyl-CoA binding By similarity
Sites
	Binding site	14	1	Acyl-CoA By similarity
	Binding site	51	1	Acyl-CoA By similarity
	Binding site	55	1	Acyl-CoA By similarity
	Binding site	74	1	Acyl-CoA By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.7
	Modified residue	8	1	N6-acetyllysine By similarity
	Modified residue	29	1	Phosphotyrosine By similarity
	Modified residue	55	1	N6-acetyllysine; alternate By similarity
	Modified residue	55	1	N6-malonyllysine; alternate By similarity
	Modified residue	77	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11030 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C6B231C903098CDE	FASTA	87	10,027
	10 20 30 40 50 60 MSQADFDKAA EEVKRLKTQP TDEEMLFIYS HFKQATVGDV NTDRPGLLDL KGKAKWDSWN 70 80 KLKGTSKENA MKTYVEKVEE LKKKYGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Putative diazepam binding inhibitor peptide: cDNA clones from rat." Mocchetti I., Einstein R., Brosius J. Proc. Natl. Acad. Sci. U.S.A. 83:7221-7225(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Molecular biology of diazepam binding inhibitor." Gray P.W. Neuropharmacology 26:863-865(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family." Mandrup S., Hummel R., Ravn S., Jensen G., Andreasen P.H., Gregersen N., Knudsen J., Kristiansen K. J. Mol. Biol. 228:1011-1022(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[4]	"Structure of the rat gene encoding the multifunctional acyl-CoA-binding protein: conservation of intron 1 sequences in rodents and man." Kroell J.B., Nohr J., Gregersen N., Kristiansen K., Mandrup S. Gene 173:239-240(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[6]	"Cloning and tissue-specific functional characterization of the promoter of the rat diazepam binding inhibitor, a peptide with multiple biological actions." Kolmer M., Alho H., Costa E., Pani L. Proc. Natl. Acad. Sci. U.S.A. 90:8439-8443(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[7]	"Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence." Knudsen J., Hoejrup P., Hansen H.O., Hansen H.F., Roepstorff P. Biochem. J. 262:513-519(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-87, ACETYLATION AT SER-2. Strain: Sprague-Dawley. Tissue: Liver.
[8]	"Isolation and characterization of a rat brain triakontatetraneuropeptide, a posttranslational product of diazepam binding inhibitor: specific action at the Ro 5-4864 recognition site." Slobodyansky E., Guidotti A., Wambebe C., Berkovich A., Costa E. J. Neurochem. 53:1276-1284(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-51. Tissue: Brain.
[9]	Lubec G., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 34-51, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14201 mRNA. Translation: AAA41078.1. M20268 mRNA. Translation: AAA41079.1. Z11991 Genomic DNA. No translation available. S51442, S51427, S51426 Genomic DNA. Translation: AAA12824.1. X96560 Genomic DNA. Translation: CAA65396.1. BC084717 mRNA. Translation: AAH84717.1. Z21846 Genomic DNA. Translation: CAA79894.1.
IPI	IPI00231069.
PIR	NZRT. S27345.
RefSeq	NP_114054.1. NM_031853.4.
UniGene	Rn.3285.
3D structure databases
ProteinModelPortal	P11030.
SMR	P11030. Positions 2-87.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1164N.
MINT	MINT-4577042.
PTM databases
PhosphoSite	P11030.
Proteomic databases
PRIDE	P11030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25045.
KEGG	rno:25045.
Organism-specific databases
CTD	1622.
RGD	2490. Dbi.
Phylogenomic databases
eggNOG	COG4281.
HOVERGEN	HBG000398.
KO	K08762.
Gene expression databases
Genevestigator	P11030.
Family and domain databases
Gene3D	1.20.80.10. 1 hit.
InterPro	IPR022408. Acyl-CoA-binding_prot_CS. IPR000582. Acyl-CoA-binding_protein. IPR014352. FERM/acyl-CoA-bd_prot_3-hlx. [Graphical view]
Pfam	PF00887. ACBP. 1 hit. [Graphical view]
PRINTS	PR00689. ACOABINDINGP.
SUPFAM	SSF47027. ACBP. 1 hit.
PROSITE	PS00880. ACB_1. 1 hit. PS51228. ACB_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605219.

Entry information

Entry name

ACBP_RAT

Accession

Primary (citable) accession number: P11030
Secondary accession number(s): Q2V4X5, Q63160

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program