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Protein

Acyl-CoA-binding protein

Gene

Dbi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Acyl-CoABy similarity
Binding sitei51 – 511Acyl-CoABy similarity
Binding sitei55 – 551Acyl-CoABy similarity
Binding sitei74 – 741Acyl-CoABy similarity

GO - Molecular functioni

  • benzodiazepine receptor binding Source: RGD
  • fatty-acyl-CoA binding Source: RGD
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • acyl-CoA metabolic process Source: RGD
  • brain development Source: RGD
  • cellular response to nutrient Source: RGD
  • glial cell proliferation Source: RGD
  • negative regulation of fatty acid beta-oxidation Source: RGD
  • pancreatic juice secretion Source: RGD
  • positive regulation of lipid biosynthetic process Source: RGD
  • positive regulation of release of sequestered calcium ion into cytosol Source: RGD
  • regulation of synaptic transmission, GABAergic Source: MGI
  • steroid biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA-binding protein
Short name:
ACBP
Alternative name(s):
Diazepam-binding inhibitor
Short name:
DBI
Endozepine
Short name:
EP
Cleaved into the following 2 chains:
Gene namesi
Name:Dbi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2490. Dbi.

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Golgi apparatus By similarity

  • Note: Golgi localization is dependent on ligand binding.By similarity

GO - Cellular componenti

  • contractile fiber Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • extracellular space Source: RGD
  • Golgi apparatus Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • plasma membrane Source: RGD
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 8786Acyl-CoA-binding proteinPRO_0000045273Add
BLAST
Peptidei18 – 5134Triakontatetraneuropeptide1 PublicationPRO_0000000288Add
BLAST
Peptidei34 – 5118OctadecaneuropeptidePRO_0000000289Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei8 – 81N6-acetyllysine; alternateBy similarity
Modified residuei8 – 81N6-succinyllysine; alternateBy similarity
Modified residuei17 – 171N6-succinyllysineBy similarity
Modified residuei29 – 291PhosphotyrosineBy similarity
Modified residuei51 – 511N6-acetyllysineBy similarity
Modified residuei55 – 551N6-acetyllysine; alternateBy similarity
Modified residuei55 – 551N6-malonyllysine; alternateBy similarity
Modified residuei55 – 551N6-succinyllysine; alternateBy similarity
Modified residuei77 – 771N6-acetyllysine; alternateBy similarity
Modified residuei77 – 771N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11030.
PRIDEiP11030.

PTM databases

iPTMnetiP11030.
PhosphoSiteiP11030.

Expressioni

Gene expression databases

ExpressionAtlasiP11030. baseline and differential.
GenevisibleiP11030. RN.

Interactioni

Subunit structurei

Monomer.

GO - Molecular functioni

  • benzodiazepine receptor binding Source: RGD

Protein-protein interaction databases

BioGridi247122. 1 interaction.
DIPiDIP-1164N.
MINTiMINT-4577042.
STRINGi10116.ENSRNOP00000066874.

Structurei

3D structure databases

ProteinModelPortaliP11030.
SMRiP11030. Positions 2-87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8786ACBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 335Acyl-CoA bindingBy similarity

Sequence similaritiesi

Belongs to the ACBP family.Curated
Contains 1 ACB (acyl-CoA-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0817. Eukaryota.
COG4281. LUCA.
GeneTreeiENSGT00840000129776.
HOVERGENiHBG000398.
InParanoidiP11030.
KOiK08762.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
[Graphical view]
PRINTSiPR00689. ACOABINDINGP.
SUPFAMiSSF47027. SSF47027. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQADFDKAA EEVKRLKTQP TDEEMLFIYS HFKQATVGDV NTDRPGLLDL
60 70 80
KGKAKWDSWN KLKGTSKENA MKTYVEKVEE LKKKYGI
Length:87
Mass (Da):10,027
Last modified:January 23, 2007 - v3
Checksum:iC6B231C903098CDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14201 mRNA. Translation: AAA41078.1.
M20268 mRNA. Translation: AAA41079.1.
Z11991 Genomic DNA. No translation available.
S51442, S51427, S51426 Genomic DNA. Translation: AAA12824.1.
X96560 Genomic DNA. Translation: CAA65396.1.
BC084717 mRNA. Translation: AAH84717.1.
Z21846 Genomic DNA. Translation: CAA79894.1.
PIRiS27345. NZRT.
RefSeqiNP_114054.1. NM_031853.4.
UniGeneiRn.3285.

Genome annotation databases

EnsembliENSRNOT00000074685; ENSRNOP00000067623; ENSRNOG00000046889.
GeneIDi25045.
KEGGirno:25045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14201 mRNA. Translation: AAA41078.1.
M20268 mRNA. Translation: AAA41079.1.
Z11991 Genomic DNA. No translation available.
S51442, S51427, S51426 Genomic DNA. Translation: AAA12824.1.
X96560 Genomic DNA. Translation: CAA65396.1.
BC084717 mRNA. Translation: AAH84717.1.
Z21846 Genomic DNA. Translation: CAA79894.1.
PIRiS27345. NZRT.
RefSeqiNP_114054.1. NM_031853.4.
UniGeneiRn.3285.

3D structure databases

ProteinModelPortaliP11030.
SMRiP11030. Positions 2-87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247122. 1 interaction.
DIPiDIP-1164N.
MINTiMINT-4577042.
STRINGi10116.ENSRNOP00000066874.

PTM databases

iPTMnetiP11030.
PhosphoSiteiP11030.

Proteomic databases

PaxDbiP11030.
PRIDEiP11030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000074685; ENSRNOP00000067623; ENSRNOG00000046889.
GeneIDi25045.
KEGGirno:25045.

Organism-specific databases

CTDi1622.
RGDi2490. Dbi.

Phylogenomic databases

eggNOGiKOG0817. Eukaryota.
COG4281. LUCA.
GeneTreeiENSGT00840000129776.
HOVERGENiHBG000398.
InParanoidiP11030.
KOiK08762.

Miscellaneous databases

PROiP11030.

Gene expression databases

ExpressionAtlasiP11030. baseline and differential.
GenevisibleiP11030. RN.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
[Graphical view]
PRINTSiPR00689. ACOABINDINGP.
SUPFAMiSSF47027. SSF47027. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Putative diazepam binding inhibitor peptide: cDNA clones from rat."
    Mocchetti I., Einstein R., Brosius J.
    Proc. Natl. Acad. Sci. U.S.A. 83:7221-7225(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Molecular biology of diazepam binding inhibitor."
    Gray P.W.
    Neuropharmacology 26:863-865(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family."
    Mandrup S., Hummel R., Ravn S., Jensen G., Andreasen P.H., Gregersen N., Knudsen J., Kristiansen K.
    J. Mol. Biol. 228:1011-1022(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  4. "Structure of the rat gene encoding the multifunctional acyl-CoA-binding protein: conservation of intron 1 sequences in rodents and man."
    Kroell J.B., Nohr J., Gregersen N., Kristiansen K., Mandrup S.
    Gene 173:239-240(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  6. "Cloning and tissue-specific functional characterization of the promoter of the rat diazepam binding inhibitor, a peptide with multiple biological actions."
    Kolmer M., Alho H., Costa E., Pani L.
    Proc. Natl. Acad. Sci. U.S.A. 90:8439-8443(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
  7. "Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence."
    Knudsen J., Hoejrup P., Hansen H.O., Hansen H.F., Roepstorff P.
    Biochem. J. 262:513-519(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-87, ACETYLATION AT SER-2.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  8. "Isolation and characterization of a rat brain triakontatetraneuropeptide, a posttranslational product of diazepam binding inhibitor: specific action at the Ro 5-4864 recognition site."
    Slobodyansky E., Guidotti A., Wambebe C., Berkovich A., Costa E.
    J. Neurochem. 53:1276-1284(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-51.
    Tissue: Brain.
  9. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 34-51, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiACBP_RAT
AccessioniPrimary (citable) accession number: P11030
Secondary accession number(s): Q2V4X5, Q63160
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.