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Protein

NAD(P) transhydrogenase, mitochondrial

Gene

NNT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi229 – 259NADBy similarityAdd BLAST31
Nucleotide bindingi965 – 970NADP6
Nucleotide bindingi1007 – 1011NADP5
Nucleotide bindingi1042 – 1049NADP8
Nucleotide bindingi1068 – 1069NADP2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP

Enzyme and pathway databases

ReactomeiR-BTA-71403 Citric acid cycle (TCA cycle)

Protein family/group databases

TCDBi3.D.2.3.1 the proton-translocating transhydrogenase (pth) family

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase, mitochondrial (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase
Pyridine nucleotide transhydrogenase
Gene namesi
Name:NNT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 20

Organism-specific databases

VGNCiVGNC:32143 NNT

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini44 – 474Mitochondrial matrix1 PublicationAdd BLAST431
Transmembranei475 – 493HelicalSequence analysisAdd BLAST19
Transmembranei501 – 521HelicalSequence analysisAdd BLAST21
Transmembranei527 – 546HelicalSequence analysisAdd BLAST20
Transmembranei558 – 578HelicalSequence analysisAdd BLAST21
Topological domaini579 – 595Mitochondrial matrix1 PublicationAdd BLAST17
Transmembranei596 – 616HelicalSequence analysisAdd BLAST21
Transmembranei622 – 642HelicalSequence analysisAdd BLAST21
Transmembranei646 – 666HelicalSequence analysisAdd BLAST21
Transmembranei672 – 691HelicalSequence analysisAdd BLAST20
Transmembranei702 – 722HelicalSequence analysisAdd BLAST21
Topological domaini723 – 739Cytoplasmic1 PublicationAdd BLAST17
Transmembranei740 – 760HelicalSequence analysisAdd BLAST21
Transmembranei778 – 797HelicalSequence analysisAdd BLAST20
Transmembranei801 – 819HelicalSequence analysisAdd BLAST19
Transmembranei833 – 853HelicalSequence analysisAdd BLAST21
Transmembranei857 – 879HelicalSequence analysisAdd BLAST23
Topological domaini880 – 1086Mitochondrial matrix1 PublicationAdd BLAST207

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Mitochondrion1 PublicationAdd BLAST43
ChainiPRO_000000105444 – 1086NAD(P) transhydrogenase, mitochondrialAdd BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei117N6-succinyllysineBy similarity1
Modified residuei224N6-succinyllysineBy similarity1
Modified residuei294N6-succinyllysineBy similarity1
Modified residuei331N6-succinyllysineBy similarity1
Modified residuei397N6-acetyllysineBy similarity1
Modified residuei1079N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP11024
PeptideAtlasiP11024
PRIDEiP11024

Expressioni

Gene expression databases

BgeeiENSBTAG00000011885

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015769

Structurei

Secondary structure

11086
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi914 – 923Combined sources10
Beta strandi925 – 931Combined sources7
Helixi933 – 937Combined sources5
Turni938 – 940Combined sources3
Helixi941 – 953Combined sources13
Beta strandi957 – 962Combined sources6
Beta strandi967 – 969Combined sources3
Helixi972 – 980Combined sources9
Helixi984 – 986Combined sources3
Beta strandi987 – 989Combined sources3
Helixi990 – 993Combined sources4
Helixi994 – 999Combined sources6
Beta strandi1001 – 1007Combined sources7
Helixi1010 – 1012Combined sources3
Helixi1015 – 1018Combined sources4
Turni1023 – 1026Combined sources4
Helixi1032 – 1034Combined sources3
Beta strandi1038 – 1045Combined sources8
Helixi1055 – 1058Combined sources4
Beta strandi1062 – 1067Combined sources6
Helixi1069 – 1081Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D4OX-ray1.21A903-1086[»]
ProteinModelPortaliP11024
SMRiP11024
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11024

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the AlaDH/PNT family.Curated
In the C-terminal section; belongs to the PNT beta subunit family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEMU Eukaryota
COG1282 LUCA
COG3288 LUCA
GeneTreeiENSGT00390000004624
HOGENOMiHOG000160623
HOVERGENiHBG006511
InParanoidiP11024
KOiK00323
OMAiKPGIPYK
OrthoDBiEOG091G01NO
TreeFamiTF300636

Family and domain databases

InterProiView protein in InterPro
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR036291 NAD(P)-bd_dom_sf
IPR026255 NADP_transhyd_a
IPR024605 NADP_transhyd_a_C
IPR034300 PNTB-like
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PF02233 PNTB, 1 hit
PF12769 PNTB_4TM, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00561 pntA, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLLKTVVT GCSCPFLSNL GSCKVLPGKK NFLRTFHTHR ILWCSAPVKP
60 70 80 90 100
GIPYKQLTVG VPKEIFQNEK RVALSPAGVQ ALVKQGFNVV VESGAGEASK
110 120 130 140 150
FSDDHYRAAG AQIQGAKEVL ASDLVVKVRA PMLNPTLGVH EADLLKTSGT
160 170 180 190 200
LISFIYPAQN PDLLNKLSKR KTTVLAMDQV PRVTIAQGYD ALSSMANIAG
210 220 230 240 250
YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL ASAGAAKSMG
260 270 280 290 300
AIVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE
310 320 330 340 350
MKLFAQQCKE VDILISTALI PGKKAPILFN KEMIESMKEG SVVVDLAAEA
360 370 380 390 400
GGNFETTKPG ELYVHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS
410 420 430 440 450
PDKDNFYFEV KDDFDFGTMG HVIRGTVVMK DGQVIFPAPT PKNIPQGAPV
460 470 480 490 500
KQKTVAELEA EKAATITPFR KTMTSASVYT AGLTGILGLG IAAPNLAFSQ
510 520 530 540 550
MVTTFGLAGI VGYHTVWGVT PALHSPLMSV TNAISGLTAV GGLVLMGGHL
560 570 580 590 600
YPSTTSQGLA ALATFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL
610 620 630 640 650
PAGTFVGGYL ASLYSGYNIE QIMYLGSGLC CVGALAGLST QGTARLGNAL
660 670 680 690 700
GMIGVAGGLA ATLGGLKPCP ELLAQMSGAM ALGGTIGLTI AKRIQISDLP
710 720 730 740 750
QLVAAFHSLV GLAAVLTCIA EYIIEYPHFA TDAAANLTKI VAYLGTYIGG
760 770 780 790 800
VTFSGSLVAY GKLQGILKSA PLLLPGRHLL NAGLLAGSVG GIIPFMMDPS
810 820 830 840 850
FTTGITCLGS VSALSAVMGV TLTAAIGGAD MPVVITVLNS YSGWALCAEG
860 870 880 890 900
FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG
910 920 930 940 950
KPMEISGTHT EINLDNAIDM IREANSIIIT PGYGLCAAKA QYPIADLVKM
960 970 980 990 1000
LSEQGKKVRF GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINHDFPDT
1010 1020 1030 1040 1050
DLVLVIGAND TVNSAAQEDP NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA
1060 1070 1080
AVDNPIFYKP NTAMLLGDAK KTCDALQAKV RESYQK
Length:1,086
Mass (Da):113,854
Last modified:March 18, 2008 - v3
Checksum:iE3663B533B8E7E35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114660 mRNA Translation: AAI14661.1
J03534 mRNA Translation: AAA30660.1
M22754 mRNA Translation: AAA30717.1
L02543 mRNA Translation: AAA21440.1
PIRiA31670 DEBOXM
RefSeqiNP_776368.1, NM_173943.3
XP_005221585.1, XM_005221528.3
XP_005221586.1, XM_005221529.3
XP_005221587.1, XM_005221530.3
UniGeneiBt.5120

Genome annotation databases

EnsembliENSBTAT00000015769; ENSBTAP00000015769; ENSBTAG00000011885
GeneIDi280878
KEGGibta:280878

Similar proteinsi

Entry informationi

Entry nameiNNTM_BOVIN
AccessioniPrimary (citable) accession number: P11024
Secondary accession number(s): A4FUB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: March 18, 2008
Last modified: April 25, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health