Reviewed, UniProtKB/Swiss-Prot P11009 (KCRS_CHICK)

Names and origin

Protein names

Recommended name:
    Creatine kinase, sarcomeric mitochondrial
    EC=2.7.3.2
Alternative name(s):
    S-MtCK
    Basic-type mitochondrial creatine kinase
    Mib-CK

Gene names

Name:

CKMT2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four MTCK homodimers. Ref.4
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Tissue specificity	Expressed in the leg muscle and heart.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 39

39

Mitochondrion Ref.2 Ref.3

Chain

40 – 419

380

Creatine kinase, sarcomeric mitochondrial

PRO_0000016599

Regions

Nucleotide binding

162 – 166

5

ATP By similarity

Nucleotide binding

354 – 359

6

ATP By similarity

Sites

Binding site

225

1

ATP By similarity

Binding site

270

1

ATP By similarity

Binding site

326

1

ATP By similarity

Binding site

369

1

ATP By similarity

Experimental info

Sequence conflict

20 – 23

4

AAGS → CSRQ in AAA48688. Ref.2

Secondary structure

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419

Helix Strand Turn

Details...

Helix

50 – 53

4

Helix

63 – 67

5

Helix

70 – 76

7

Helix

87 – 96

10

Helix

115 – 118

4

Helix

120 – 130

11

Turn

131 – 133

3

Turn

136 – 138

3

Helix

147 – 149

3

Turn

157 – 159

3

Beta strand

160 – 169

10

Turn

177 – 179

3

Helix

182 – 197

16

Helix

201 – 203

3

Beta strand

205 – 212

8

Helix

215 – 223

9

Helix

234 – 237

4

Turn

238 – 240

3

Turn

243 – 248

6

Beta strand

250 – 254

5

Beta strand

259 – 278

20

Helix

280 – 300

21

Turn

309 – 311

3

Helix

318 – 320

3

Beta strand

326 – 332

7

Helix

334 – 338

5

Helix

342 – 349

8

Beta strand

351 – 354

4

Turn

359 – 363

5

Beta strand

367 – 372

6

Beta strand

375 – 378

4

Helix

380 – 402

23

Sequences

	Sequence		Length	Mass (Da)
	P11009-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 9ACC60D709B9283F	FASTA	419	47,084
	10 20 30 40 50 60 MAGTFGRLLA GRVTAALFAA AGSGVLTTGY LLNQQNVKAT VHEKRKLFPP SADYPDLRKH 70 80 90 100 110 120 NNCMAECLTP AIYAKLRDKL TPNGYSLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 130 140 150 160 170 180 EIFDPVIKAR HNGYDPRTMK HHTDLDASKI THGQFDERYV LSSRVRTGRS IRGLSLPPAC 190 200 210 220 230 240 SRAERREVEN VVVTALAGLK GDLSGKYYSL TNMSERDQQQ LIDDHFLFDK PVSPLLTCAG 250 260 270 280 290 300 MARDWPDARG IWHNNDKTFL VWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIKE 310 320 330 340 350 360 RGWEFMWNER LGYVLTCPSN LGTGLRAGVH VKLPRLSKDP RFPKILENLR LQKRGTGGVD 370 380 390 400 410 TAAVADVYDI SNLDRMGRSE VELVQIVIDG VNYLVDCEKK LEKGQDIKVP PPLPQFGRK

References

[1]	"Evolution of the creative kinases. The chicken acidic type mitochondrial creatine kinase gene as the first nonmammalian gene." Muehlebach S.M., Wirz T., Braendle U., Perriard J.-C. J. Biol. Chem. 271:11920-11929(1996) [PubMed: 8662608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-39. Strain: White leghorn.
[2]	"Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework." Hossle J.P., Schlegel J., Wegmann G., Wyss M., Boehlen P., Eppenberger H.M., Wallimann T., Perriard J.-C. Biochem. Biophys. Res. Commun. 151:408-416(1988) [PubMed: 2831887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-419, PROTEIN SEQUENCE OF 40-69. Tissue: Heart and Skeletal muscle.
[3]	"The N-terminal heptapeptide of mitochondrial creatine kinase is important for octamerization." Kaldis P., Furter R., Wallimann T. Biochemistry 33:952-959(1994) [PubMed: 8305443] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-48.
[4]	"Structure of mitochondrial creatine kinase." Fritz-Wolf K., Schnyder T., Wallimann T., Kabsch W. Nature 381:341-345(1996) [PubMed: 8692275] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M18866 mRNA. Translation: AAA48688.1.

IPI

IPI00585452.

PIR

A27708.

UniGene

Gga.869

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CRK	X-ray	3.00	A/B/C/D	40-419	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSGALG00000015602. Gallus gallus. [Contig view]

Phylogenomic databases

HOGENOM

P11009.

HOVERGEN

P11009.

Enzyme and pathway databases

BRENDA

2.7.3.2. 4.

Family and domain databases

InterPro

IPR000749. ATP-guanido_PTrfase.
IPR014746. Gln_synth/guanido_kin_cat.
[Graphical view]

Gene3D

G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit.
G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.

PANTHER

PTHR11547. ATP-gua_Ptrans. 1 hit.

Pfam

PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]

PROSITE

PS00112. GUANIDO_KINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KCRS_CHICK

Accession

Primary (citable) accession number: P11009

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)