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P11003

- METF_SALTY

UniProt

P11003 - METF_SALTY

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei88 – 881FADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity
    Binding sitei279 – 2791SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi118 – 1203FADBy similarity
    Nucleotide bindingi131 – 1322FADBy similarity
    Nucleotide bindingi156 – 1594FADBy similarity
    Nucleotide bindingi165 – 1728FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-4135-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:STM4105
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190264Add
    BLAST

    Proteomic databases

    PaxDbiP11003.
    PRIDEiP11003.

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM4105.

    Structurei

    3D structure databases

    ProteinModelPortaliP11003.
    SMRiP11003. Positions 3-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246232.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.
    PhylomeDBiP11003.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P11003-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFFHANQRE ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS    50
    LKPKFVSVTY GANSGERDRT HSVIKGIKER TGLEAAPHLT CIDATRDELR 100
    TIARDYWNNG IRHIVALRGD LPPGSGKPEM YAADLVGLLK EVADFDISVA 150
    AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS 200
    AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPSWMSLMFE GLDNDAETRK 250
    LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL 296
    Length:296
    Mass (Da):33,173
    Last modified:January 23, 2002 - v2
    Checksum:i2B834F880B56A643
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941A → P in CAA30531. (PubMed:2841568)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07689 Genomic DNA. Translation: CAA30531.1.
    AE006468 Genomic DNA. Translation: AAL22945.1.
    PIRiS03169.
    RefSeqiNP_462986.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL22945; AAL22945; STM4105.
    GeneIDi1255632.
    KEGGistm:STM4105.
    PATRICi32387096. VBISalEnt20916_4328.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07689 Genomic DNA. Translation: CAA30531.1 .
    AE006468 Genomic DNA. Translation: AAL22945.1 .
    PIRi S03169.
    RefSeqi NP_462986.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P11003.
    SMRi P11003. Positions 3-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM4105.

    Proteomic databases

    PaxDbi P11003.
    PRIDEi P11003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL22945 ; AAL22945 ; STM4105 .
    GeneIDi 1255632.
    KEGGi stm:STM4105.
    PATRICi 32387096. VBISalEnt20916_4328.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246232.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.
    PhylomeDBi P11003.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci SENT99287:GCTI-4135-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli."
      Stauffer G.V., Stauffer L.T.
      Mol. Gen. Genet. 212:246-251(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiMETF_SALTY
    AccessioniPrimary (citable) accession number: P11003
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3