Reviewed, UniProtKB/Swiss-Prot P11003 (METF_SALTY)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	STM4105

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	5,10-methylenetetrahydrofolate reductase		PRO_0000190264
Experimental info
	Sequence conflict	94	1	A → P in CAA30531. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11003-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 2B834F880B56A643	FASTA	296	33,173
	10 20 30 40 50 60 MSFFHANQRE ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY 70 80 90 100 110 120 GANSGERDRT HSVIKGIKER TGLEAAPHLT CIDATRDELR TIARDYWNNG IRHIVALRGD 130 140 150 160 170 180 LPPGSGKPEM YAADLVGLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA 190 200 210 220 230 240 ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPSWMSLMFE 250 260 270 280 290 GLDNDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli." Stauffer G.V., Stauffer L.T. Mol. Gen. Genet. 212:246-251(1988) [PubMed: 2841568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07689 Genomic DNA. Translation: CAA30531.1. AE008891 Genomic DNA. Translation: AAL22945.1.
PIR	S03169.
RefSeq	NP_462986.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
SMR	P11003. Positions 3-294.
ModBase	Search...
Genome annotation databases
GeneID	1255632.
GenomeReviews	Gene locus STM4105 in contig AE006468_GR.
KEGG	stm:STM4105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P11003.
OMA	P11003. FFPPKTP.
Enzyme and pathway databases
BioCyc	STYP99287:STM4105-MON.
BRENDA	1.5.1.20. 2.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_SALTY

Accession

Primary (citable) accession number: P11003

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2002
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)