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P11003

- METF_SALTY

UniProt

P11003 - METF_SALTY

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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei88 – 881FADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity
Binding sitei279 – 2791SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi118 – 1203FADBy similarity
Nucleotide bindingi131 – 1322FADBy similarity
Nucleotide bindingi156 – 1594FADBy similarity
Nucleotide bindingi165 – 1728FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4135-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:STM4105
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190264Add
BLAST

Proteomic databases

PaxDbiP11003.
PRIDEiP11003.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4105.

Structurei

3D structure databases

ProteinModelPortaliP11003.
SMRiP11003. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.
PhylomeDBiP11003.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P11003 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFFHANQRE ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS
60 70 80 90 100
LKPKFVSVTY GANSGERDRT HSVIKGIKER TGLEAAPHLT CIDATRDELR
110 120 130 140 150
TIARDYWNNG IRHIVALRGD LPPGSGKPEM YAADLVGLLK EVADFDISVA
160 170 180 190 200
AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS
210 220 230 240 250
AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPSWMSLMFE GLDNDAETRK
260 270 280 290
LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL
Length:296
Mass (Da):33,173
Last modified:January 23, 2002 - v2
Checksum:i2B834F880B56A643
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941A → P in CAA30531. (PubMed:2841568)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07689 Genomic DNA. Translation: CAA30531.1.
AE006468 Genomic DNA. Translation: AAL22945.1.
PIRiS03169.
RefSeqiNP_462986.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22945; AAL22945; STM4105.
GeneIDi1255632.
KEGGistm:STM4105.
PATRICi32387096. VBISalEnt20916_4328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07689 Genomic DNA. Translation: CAA30531.1 .
AE006468 Genomic DNA. Translation: AAL22945.1 .
PIRi S03169.
RefSeqi NP_462986.1. NC_003197.1.

3D structure databases

ProteinModelPortali P11003.
SMRi P11003. Positions 3-294.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM4105.

Proteomic databases

PaxDbi P11003.
PRIDEi P11003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL22945 ; AAL22945 ; STM4105 .
GeneIDi 1255632.
KEGGi stm:STM4105.
PATRICi 32387096. VBISalEnt20916_4328.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246232.
KOi K00297.
OMAi FEFFPTK.
OrthoDBi EOG6D2KVW.
PhylomeDBi P11003.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci SENT99287:GCTI-4135-MONOMER.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli."
    Stauffer G.V., Stauffer L.T.
    Mol. Gen. Genet. 212:246-251(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiMETF_SALTY
AccessioniPrimary (citable) accession number: P11003
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2002
Last modified: October 1, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3