P11003 (METF_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydrofolate reductase
EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	STM4105

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	5,10-methylenetetrahydrofolate reductase		PRO_0000190264
Experimental info
	Sequence conflict	94	1	A → P in CAA30531. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11003 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 2B834F880B56A643	FASTA	296	33,173
	10 20 30 40 50 60 MSFFHANQRE ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY 70 80 90 100 110 120 GANSGERDRT HSVIKGIKER TGLEAAPHLT CIDATRDELR TIARDYWNNG IRHIVALRGD 130 140 150 160 170 180 LPPGSGKPEM YAADLVGLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA 190 200 210 220 230 240 ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPSWMSLMFE 250 260 270 280 290 GLDNDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli." Stauffer G.V., Stauffer L.T. Mol. Gen. Genet. 212:246-251(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07689 Genomic DNA. Translation: CAA30531.1. AE006468 Genomic DNA. Translation: AAL22945.1.
PIR	S03169.
RefSeq	NP_462986.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P11003.
SMR	P11003. Positions 3-294.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM4105.
Proteomic databases
PaxDb	P11003.
PRIDE	P11003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL22945; AAL22945; STM4105.
GeneID	1255632.
KEGG	stm:STM4105.
PATRIC	32387096. VBISalEnt20916_4328.
Phylogenomic databases
eggNOG	COG0685.
HOGENOM	HOG000246232.
KO	K00297.
OMA	SITQMFF.
ProtClustDB	PRK09432.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_SALTY

Accession

Primary (citable) accession number: P11003

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program