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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei88 – 881FADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity
Binding sitei279 – 2791SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi118 – 1203FADBy similarity
Nucleotide bindingi131 – 1322FADBy similarity
Nucleotide bindingi156 – 1594FADBy similarity
Nucleotide bindingi165 – 1728FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4135-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:STM4105
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190264Add
BLAST

Proteomic databases

PaxDbiP11003.
PRIDEiP11003.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4105.

Structurei

3D structure databases

ProteinModelPortaliP11003.
SMRiP11003. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.
PhylomeDBiP11003.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P11003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFHANQRE ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS
60 70 80 90 100
LKPKFVSVTY GANSGERDRT HSVIKGIKER TGLEAAPHLT CIDATRDELR
110 120 130 140 150
TIARDYWNNG IRHIVALRGD LPPGSGKPEM YAADLVGLLK EVADFDISVA
160 170 180 190 200
AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS
210 220 230 240 250
AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPSWMSLMFE GLDNDAETRK
260 270 280 290
LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL
Length:296
Mass (Da):33,173
Last modified:January 23, 2002 - v2
Checksum:i2B834F880B56A643
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941A → P in CAA30531 (PubMed:2841568).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07689 Genomic DNA. Translation: CAA30531.1.
AE006468 Genomic DNA. Translation: AAL22945.1.
PIRiS03169.
RefSeqiNP_462986.1. NC_003197.1.
WP_000007508.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22945; AAL22945; STM4105.
GeneIDi1255632.
KEGGistm:STM4105.
PATRICi32387096. VBISalEnt20916_4328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07689 Genomic DNA. Translation: CAA30531.1.
AE006468 Genomic DNA. Translation: AAL22945.1.
PIRiS03169.
RefSeqiNP_462986.1. NC_003197.1.
WP_000007508.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP11003.
SMRiP11003. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM4105.

Proteomic databases

PaxDbiP11003.
PRIDEiP11003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL22945; AAL22945; STM4105.
GeneIDi1255632.
KEGGistm:STM4105.
PATRICi32387096. VBISalEnt20916_4328.

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.
PhylomeDBiP11003.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSENT99287:GCTI-4135-MONOMER.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli."
    Stauffer G.V., Stauffer L.T.
    Mol. Gen. Genet. 212:246-251(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiMETF_SALTY
AccessioniPrimary (citable) accession number: P11003
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2002
Last modified: May 27, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.