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Reviewed, UniProtKB/Swiss-Prot P11001 (GTF1_STRDO)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glucosyltransferase-I
      Short name=GTF-I
    EC=2.4.1.5
Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase
Gene names
Name: gtfI
OrganismStreptococcus downei (Streptococcus sobrinus)
Taxonomic identifier1317 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

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Protein attributes

Sequence length1597 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activity

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

Subcellular location

Secreted.

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 70 family.

Contains 24 cell wall-binding repeats.

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Ontologies

Keywords
   Cellular componentSecreted
   DiseaseDental caries
   DomainRepeat
Signal
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Biological processglucan biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncation binding

Inferred from electronic annotation. Source: InterPro

dextransucrase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential
Chain39 – 15971559Glucosyltransferase-I
PRO_0000021383

Regions

Repeat157 – 17620Cell wall-binding 1
Repeat178 – 19720Cell wall-binding 2
Repeat1089 – 110820Cell wall-binding 3
Repeat1109 – 112820Cell wall-binding 4
Repeat1130 – 115021Cell wall-binding 5
Repeat1152 – 117221Cell wall-binding 6
Repeat1173 – 119119Cell wall-binding 7
Repeat1193 – 121422Cell wall-binding 8
Repeat1216 – 123621Cell wall-binding 9
Repeat1237 – 125620Cell wall-binding 10
Repeat1258 – 127922Cell wall-binding 11
Repeat1281 – 130121Cell wall-binding 12
Repeat1302 – 132120Cell wall-binding 13
Repeat1323 – 134321Cell wall-binding 14
Repeat1344 – 136522Cell wall-binding 15
Repeat1366 – 138015Cell wall-binding 16
Repeat1415 – 143420Cell wall-binding 17
Repeat1436 – 145722Cell wall-binding 18
Repeat1459 – 147820Cell wall-binding 19
Repeat1485 – 150521Cell wall-binding 20
Repeat1508 – 152720Cell wall-binding 21
Repeat1528 – 154720Cell wall-binding 22
Repeat1549 – 157022Cell wall-binding 23
Repeat1572 – 159120Cell wall-binding 24
Region200 – 1050851Catalytic; approximate
Region1099 – 1597499Glucan-binding; approximate

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Sequences

Sequence LengthMass (Da)Tools
P11001-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: B9E86A200868798E

FASTA1,597177,080
        10         20         30         40         50         60 
MEKNERFKMH KVKKRWVTIS VASATMLASA LGASVASADT ETVSEDSNQA VLTADQTTTN 

        70         80         90        100        110        120 
QDTEQTSVAA TATSEQSAST DAATDQASAT DQASAAEQTQ GTTASTDTAA QTTTNANEAK 

       130        140        150        160        170        180 
WVPTENENQV FTDEMLAEAK NVATAESNSI PSDLAKMSNV KQVDGKYYYY DQDGNVKKNF 

       190        200        210        220        230        240 
AVSVGEKIYY FDETGAYKDT SKVEADKSGS DISKEETTFA ANNRAYSTSA ENFEAIDNYL 

       250        260        270        280        290        300 
TADSWYRPKS ILKDGKTWTE SSKDDFRPLL MAWWPDTETK RNYVNYMNKV VGIDKTYTAE 

       310        320        330        340        350        360 
TSQADLTAAA ELVQARIEQK ITTEQNTKWL REAISAFVKT QPQWNGESEK PYDDHLQNGA 

       370        380        390        400        410        420 
LKFDNQSDLT PDTQSNYRLL NRTPTNQTGS LDSRFTYNAN DPLGGYELLL ANDVDNSNPI 

       430        440        450        460        470        480 
VQAEQLNWLH YLLNFGTIYA KDADANFDSI RVDAVDNVDA DLLQISSDYL KAAYGIDKNN 

       490        500        510        520        530        540 
KNANNHVSIV EAWSDNDTPY LHDDGDNLMN MDNKFRLSML WSLAKPLDKR SGLNPLIHNS 

       550        560        570        580        590        600 
LVDREVDDRE VETVPSYSFA RAHDSEVQDL IRDIIKAEIN PNAFGYSFTQ DEIDQAFKIY 

       610        620        630        640        650        660 
NEDLKKTDKK YTHYNVPLSY TLLLTNKGSI PRVYYGDMFT DDGQYMANKT VNYDAIESLL 

       670        680        690        700        710        720 
KARMKYVAGG QAMQNYQIGN GEILTSVRYG KGALKQSDKG DATTRTSGVG VVMGNQPNFS 

       730        740        750        760        770        780 
LDGKVVALNM GAAHANQEYR ALMVSTKDGV ATYATDADAS KAGLVKRTDE NGYLYFLNDD 

       790        800        810        820        830        840 
LKGVANPQVS GFLQVWVPVG AADDQDIRVA ASDTASTDGK SLHQDAAMDS RVMFEGFSNF 

       850        860        870        880        890        900 
QSFATKEEEY TNVVIANNVD KFVSWGITDF EMAPQYVSST DGQFLDSVIQ NGYAFTDRYD 

       910        920        930        940        950        960 
LGMSKANKYG TADQLVKAIK ALHAKGLKVM ADWVPDQMYT FPKQEVVTVT RTDKFGKPIA 

       970        980        990       1000       1010       1020 
GSQINHSLYV TDTKSSGDDY QAKYGGAFLD ELKEKYPELF TKKQISTGQA IDPSVKIKQW 

      1030       1040       1050       1060       1070       1080 
SAKYFNGSNI LGRGADYVLS DQASNKYLNV SDDKLFLPKT LLGQVVESGI RFDGTGYVYN 

      1090       1100       1110       1120       1130       1140 
SSTTGEKVTD SFITEAGNLY YFGQDGYMVT GAQNIKGSNY YFLANGAALR NTVYTDAQGQ 

      1150       1160       1170       1180       1190       1200 
NHYYGNDGKR YENGYQQFGN DSWRYFKNGV MALGLTTVDG HVQYFDKDGV QAKDKIIVTR 

      1210       1220       1230       1240       1250       1260 
DGKVRYFDQH NGNAVTNTFV ADKTGHWYYL GKDGVAVTGA QTVGKQHLYF EANGQQVKGD 

      1270       1280       1290       1300       1310       1320 
FVTAKDGKLY FYDVDSGDMW TNTFIEDKAG NWFYLGKDGA AVTGAQTIKG QKLYFKANGQ 

      1330       1340       1350       1360       1370       1380 
QVKGDIVKDA DGKIRYYDAQ TGEQVFNKSV SVNGKTYYFG SDGTAQTQAN PKGQTFKDGS 

      1390       1400       1410       1420       1430       1440 
GVLRFYNLEG QYVSGSGWYE TAEHEWVYVK SGKVLTGAQT IGNQRVYFKD NGHQVKGQLV 

      1450       1460       1470       1480       1490       1500 
TGNDGKLRYY DANSGDQAFN KSVTVNGKTY YFGSDGTAQT QANPKGQTFK DGSGVLRFYN 

      1510       1520       1530       1540       1550       1560 
LEGQYVSGSG WYKNAQGQWL YVKDGKVLTG LQTVGNQKVY FDKNGIQAKG KAVRTSDGKV 

      1570       1580       1590 
RYFDENSGSM ITNQWKFVYG QYYYFGSDGA AVYRGWN

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References

[1]"Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28."
Ferretti J.J., Gilpin M.L., Russell R.R.B.
J. Bacteriol. 169:4271-4278(1987) [PubMed: 3040686] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MFE28.

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Cross-references

Sequence databases

M17391 Genomic DNA. Translation: AAC63063.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH70. Glycoside Hydrolase Family 70.

Enzyme and pathway databases

BRENDA2.4.1.5. 290403.

Family and domain databases

InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR002479. Cell_wall_bd_put.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
PfamPF01473. CW_binding_1. 4 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
PROSITEPS51170. CW. 15 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGTF1_STRDO
AccessionPrimary (citable) accession number: P11001
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents