P10999 (LAML3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lamin-L(III) Alternative name(s): Lamin-B3
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Subcellular location	Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Developmental stage	Expressed in oocytes.
Miscellaneous	There are at least five different lamins in Xenopus: the somatic lamins L(I), L(II), and A; the oocyte germinal vesicle lamin L(III); and the male germ cells lamin l(IV).
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Gene Ontology (GO)
Cellular component	intermediate filament Inferred from electronic annotation. Source: UniProtKB-KW nuclear inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P10999-1)

Also known as: B3A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P10999-2)

Also known as: B3B;

The sequence of this isoform differs from the canonical sequence as follows:
     572-583: SHQSVDPSCSIM → TKRRKKKCCSVS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 580	579	Lamin-L(III)		PRO_0000063825
	Propeptide	581 – 583	3	Removed in mature form By similarity		PRO_0000396784
Regions
	Region	2 – 32	31	Head
	Region	33 – 384	352	Rod
	Region	33 – 67	35	Coil 1A
	Region	68 – 79	12	Linker 1
	Region	80 – 215	136	Coil 1B
	Region	216 – 242	27	Linker 2
	Region	243 – 384	142	Coil 2
	Region	385 – 580	196	Tail
	Compositional bias	550 – 565	16	Asp/Glu-rich (highly acidic; could be involved in chromatin binding)
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	580	1	Cysteine methyl ester By similarity
	Lipidation	580	1	S-farnesyl cysteine By similarity
Natural variations
	Alternative sequence	572 – 583	12	SHQSV…SCSIM → TKRRKKKCCSVS in isoform 2.		VSP_002473
Experimental info
	Sequence conflict	349	1	Q → H in CAA31567. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B3A) [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 6BDBD1CF25ACEE92	FASTA	583	67,291
	10 20 30 40 50 60 MATSTPSRAR EHASAAQSPG SPTRISRMQE KEDLRHLNDR LAAYIERVRS LEADKSLLKI 70 80 90 100 110 120 QLEEREEVSS REVTNLRQLY ETELADARKL LDQTANERAR LQVELGKVRE EYRQLQARNS 130 140 150 160 170 180 KKENDLSLAQ NQLRDLESKL NTKEAELATA LSGKRGLEEQ LQEQRAQIAG LESSLRDTTK 190 200 210 220 230 240 QLHDEMLWRV DLENKMQTIR EQLDFQKNIH TQEVKEIKKR HDTRIVEIDS GRRVEFESKL 250 260 270 280 290 300 AEALQELRRD HEQQILEYKE HLEKNFSAKL ENAQLAAAKN SDYASATREE IMATKLRVDT 310 320 330 340 350 360 LSSQLNHYQK QNSALEAKVR DLQDMLDRAH DMHRRQMTEK DREVTEIRQT LQGQLEEYEQ 370 380 390 400 410 420 LLDVKLALDM EINAYRKMLE GEEQRLKLSP SPSQRSTVSR ASTSQTSRLL RGKKRKLDET 430 440 450 460 470 480 GRSVTKRSYK VVQQASSTGP VSVEDIDPEG NYVRLLNNTE EDFSLHGWVV KRMHMSLPEI 490 500 510 520 530 540 AFKLPCRFIL KSSQRVTIWA AGAGAVHSPP TDLVWKSQKT WGTGDNIKIT LLDSTGEECA 550 560 570 580 ERTLYRVIGE EGETDEDFVE EEELERQFRS QSHQSVDPSC SIM
	Isoform 2 (B3B) [UniParc]. Checksum: E0AF8D06E5B8B713	FASTA	583	67,425
	10 20 30 40 50 60 MATSTPSRAR EHASAAQSPG SPTRISRMQE KEDLRHLNDR LAAYIERVRS LEADKSLLKI 70 80 90 100 110 120 QLEEREEVSS REVTNLRQLY ETELADARKL LDQTANERAR LQVELGKVRE EYRQLQARNS 130 140 150 160 170 180 KKENDLSLAQ NQLRDLESKL NTKEAELATA LSGKRGLEEQ LQEQRAQIAG LESSLRDTTK 190 200 210 220 230 240 QLHDEMLWRV DLENKMQTIR EQLDFQKNIH TQEVKEIKKR HDTRIVEIDS GRRVEFESKL 250 260 270 280 290 300 AEALQELRRD HEQQILEYKE HLEKNFSAKL ENAQLAAAKN SDYASATREE IMATKLRVDT 310 320 330 340 350 360 LSSQLNHYQK QNSALEAKVR DLQDMLDRAH DMHRRQMTEK DREVTEIRQT LQGQLEEYEQ 370 380 390 400 410 420 LLDVKLALDM EINAYRKMLE GEEQRLKLSP SPSQRSTVSR ASTSQTSRLL RGKKRKLDET 430 440 450 460 470 480 GRSVTKRSYK VVQQASSTGP VSVEDIDPEG NYVRLLNNTE EDFSLHGWVV KRMHMSLPEI 490 500 510 520 530 540 AFKLPCRFIL KSSQRVTIWA AGAGAVHSPP TDLVWKSQKT WGTGDNIKIT LLDSTGEECA 550 560 570 580 ERTLYRVIGE EGETDEDFVE EEELERQFRS QTKRRKKKCC SVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene structure of nuclear lamin LIII of Xenopus laevis; a model for the evolution of IF proteins from a lamin-like ancestor." Doering V., Stick R. EMBO J. 9:4073-4081(1990) [PubMed: 2249665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis." Stick R. EMBO J. 7:3189-3197(1988) [PubMed: 3181134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Ovary.
[3]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13169 mRNA. Translation: CAA31567.1. BC078034 mRNA. Translation: AAH78034.1.
PIR	S01301.
RefSeq	NP_001081545.1. NM_001088076.1.
UniGene	Xl.1238.
3D structure databases
ProteinModelPortal	P10999.
SMR	P10999. Positions 313-385, 435-544.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397910.
KEGG	xla:397910.
Organism-specific databases
CTD	397910.
Xenbase	XB-GENE-920628. lmnb3.
Phylogenomic databases
HOVERGEN	HBG013015.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR001322. IF_tail_C. IPR018039. Intermediate_filament_CS. [Graphical view]
KO	K07611.
PANTHER	PTHR23239. IF. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF00932. IF_tail. 1 hit. [Graphical view]
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAML3_XENLA

Accession

Primary (citable) accession number: P10999
Secondary accession number(s): P23420, Q6AZG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1991
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program