Reviewed, UniProtKB/Swiss-Prot P10989 (ACT_SCHPO)

Names and origin

Protein names

Recommended name:
    Actin

Gene names

Name:	act1
Synonyms:	cps8
ORF Names:	SPBC32H8.12c, pi012

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis, contractile ring formation Inferred from genetic interaction. Source: GeneDB_SPombe establishment or maintenance of cell polarity Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe fungal-type cell wall biogenesis Inferred from mutant phenotype. Source: GeneDB_SPombe histone exchange Inferred from physical interaction. Source: GeneDB_SPombe regulation of endocytosis Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	Ino80 complex Inferred from direct assay. Source: GeneDB_SPombe NuA4 histone acetyltransferase complex Inferred from direct assay. Source: GeneDB_SPombe Swr1 complex Inferred from direct assay. Source: GeneDB_SPombe actin filament Traceable author statement. Source: GeneDB_SPombe actomyosin contractile ring Traceable author statement. Source: GeneDB_SPombe cell tip Inferred from direct assay. Source: GeneDB_SPombe prospore membrane Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Actin		PRO_0000089007
Amino acid modifications
	Modified residue	202	1	Phosphothreonine Ref.5
	Modified residue	324	1	Phosphoserine Ref.5
Experimental info
	Mutagenesis	273	1	G → D: Causes a defect in the maintenance of cell polarity and septum organization. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10989-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 372C0B2CDBAE1B68	FASTA	375	41,765
	10 20 30 40 50 60 MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH HGIMVGMGQK DSYVGDEAQS 70 80 90 100 110 120 KRGILTLKYP IEHGIVNNWD DMEKIWHHTF YNELRVAPEE HPCLLTEAPL NPKSNREKMT 130 140 150 160 170 180 QIIFETFNAP AFYVAIQAVL SLYASGRTTG IVLDSGDGVT HTVPIYEGYA LPHAIMRLDL 190 200 210 220 230 240 AGRDLTDYLM KILMERGYTF STTAEREIVR DIKEKLCYVA LDFEQELQTA AQSSSLEKSY 250 260 270 280 290 300 ELPDGQVITI GNERFRAPEA LFQPSALGLE NAGIHEATYN SIMKCDVDIR KDLYGNVVMS 310 320 330 340 350 360 GGTTMYPGIA DRMQKEIQAL APSSMKVKIV APPERKYSVW IGGSILASLS TFQQMWISKQ 370 EYDESGPGIV YRKCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A single intronless action gene in the fission yeast Schizosaccharomyces pombe: nucleotide sequence and transcripts formed in homologous and heterologous yeast." Mertins P., Gallwitz D. Nucleic Acids Res. 15:7369-7379(1987) [PubMed: 3309892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"An actin point-mutation neighboring the 'hydrophobic plug' causes defects in the maintenance of cell polarity and septum organization in the fission yeast Schizosaccharomyces pombe." Ishiguro J., Kobayashi W. FEBS Lett. 392:237-241(1996) [PubMed: 8774852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-273. Strain: ATCC 38366 / 972.
[3]	"A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment." Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M. Yeast 16:71-80(2000) [PubMed: 10620777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[4]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[5]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-202 AND SER-324, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00447 Genomic DNA. Translation: CAA68501.1. D84318 Genomic DNA. Translation: BAA12315.1. AB004534 Genomic DNA. Translation: BAA21389.1. CU329671 Genomic DNA. Translation: CAC37502.1.
PIR	A26836.
RefSeq	NP_595618.1.
3D structure databases
SMR	P10989. Positions 7-375.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10989. 4 interactions.
STRING	P10989.
Genome annotation databases
GeneID	2540051.
GenomeReviews	Gene locus act1 in contig CU329671_GR.
KEGG	spo:SPBC32H8.12c.
NMPDR	fig|4896.1.peg.1484.
Organism-specific databases
GeneDB_Spombe	SPBC32H8.12c.
Phylogenomic databases
OMA	EKMTQIL
OrthoDB	EOG94QVH3
Gene expression databases
ArrayExpress	P10989.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR004001. Actin_CS. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACT_SCHPO

Accession

Primary (citable) accession number: P10989
Secondary accession number(s): Q10288

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 24, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)