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Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).By similarity

GO - Molecular functioni

  • drug binding Source: RGD
  • G-protein coupled acetylcholine receptor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:Chrm2
Synonyms:Chrm-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620023. Chrm2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22ExtracellularBy similarityAdd BLAST22
Transmembranei23 – 45Helical; Name=1By similarityAdd BLAST23
Topological domaini46 – 59CytoplasmicBy similarityAdd BLAST14
Transmembranei60 – 80Helical; Name=2By similarityAdd BLAST21
Topological domaini81 – 97ExtracellularBy similarityAdd BLAST17
Transmembranei98 – 119Helical; Name=3By similarityAdd BLAST22
Topological domaini120 – 139CytoplasmicBy similarityAdd BLAST20
Transmembranei140 – 162Helical; Name=4By similarityAdd BLAST23
Topological domaini163 – 184ExtracellularBy similarityAdd BLAST22
Transmembranei185 – 209Helical; Name=5By similarityAdd BLAST25
Topological domaini210 – 387CytoplasmicBy similarityAdd BLAST178
Transmembranei388 – 410Helical; Name=6By similarityAdd BLAST23
Topological domaini411 – 418ExtracellularBy similarity8
Transmembranei419 – 442Helical; Name=7By similarityAdd BLAST24
Topological domaini443 – 466CytoplasmicBy similarityAdd BLAST24

GO - Cellular componenti

  • asymmetric synapse Source: RGD
  • axon terminus Source: RGD
  • cell junction Source: UniProtKB-KW
  • dendrite Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
  • symmetric synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL309.
GuidetoPHARMACOLOGYi14.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690251 – 466Muscarinic acetylcholine receptor M2Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...)Sequence analysis1
Glycosylationi3N-linked (GlcNAc...)Sequence analysis1
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Modified residuei232PhosphoserineCombined sources1
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446PhosphothreonineSequence analysis1
Modified residuei450PhosphothreonineSequence analysis1
Modified residuei465PhosphothreonineSequence analysis1

Post-translational modificationi

Phosphorylated in response to agonist treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP10980.

PTM databases

iPTMnetiP10980.
PhosphoSitePlusiP10980.

Expressioni

Gene expression databases

BgeeiENSRNOG00000011928.

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2. Interacts with RACK1; the interaction regulates CHRM2 internalization (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065178.

Chemistry databases

BindingDBiP10980.

Structurei

3D structure databases

ProteinModelPortaliP10980.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 107Agonist bindingBy similarity5
Regioni403 – 404Agonist bindingBy similarity2
Regioni426 – 430Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi120 – 122Important for signaling3
Motifi436 – 440Important for signaling5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP10980.
KOiK04130.
OMAiQNGKGPS.
OrthoDBiEOG091G06VI.
PhylomeDBiP10980.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSRDDNS KQTCIKIVTK AQKGDVCTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,539
Last modified:December 1, 2000 - v2
Checksum:i70ECCD86366A676B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51N → S in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti273N → T in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti289 – 290VS → SA in AAA40926 (PubMed:2825184).Curated2
Sequence conflicti313G → D in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti337C → Y in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti353N → S in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti360I → V in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti369T → P in AAA40926 (PubMed:2825184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03025 mRNA. Translation: AAA40926.1.
AB017655 mRNA. Translation: BAA36838.1.
PIRiJH0197.
S10856.
RefSeqiNP_112278.1. NM_031016.1.
UniGeneiRn.10752.
Rn.200974.

Genome annotation databases

EnsembliENSRNOT00000075341; ENSRNOP00000065178; ENSRNOG00000046972.
GeneIDi81645.
KEGGirno:81645.
UCSCiRGD:620023. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03025 mRNA. Translation: AAA40926.1.
AB017655 mRNA. Translation: BAA36838.1.
PIRiJH0197.
S10856.
RefSeqiNP_112278.1. NM_031016.1.
UniGeneiRn.10752.
Rn.200974.

3D structure databases

ProteinModelPortaliP10980.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065178.

Chemistry databases

BindingDBiP10980.
ChEMBLiCHEMBL309.
GuidetoPHARMACOLOGYi14.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP10980.
PhosphoSitePlusiP10980.

Proteomic databases

PaxDbiP10980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000075341; ENSRNOP00000065178; ENSRNOG00000046972.
GeneIDi81645.
KEGGirno:81645.
UCSCiRGD:620023. rat.

Organism-specific databases

CTDi1129.
RGDi620023. Chrm2.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP10980.
KOiK04130.
OMAiQNGKGPS.
OrthoDBiEOG091G06VI.
PhylomeDBiP10980.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-RNO-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiP10980.

Gene expression databases

BgeeiENSRNOG00000011928.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM2_RAT
AccessioniPrimary (citable) accession number: P10980
Secondary accession number(s): Q9Z2Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.