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P10970

- RL22_HALMA

UniProt

P10970 - RL22_HALMA

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Protein

50S ribosomal protein L22

Gene

rpl22

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.UniRule annotation
Contacts all 6 domains of the 23S rRNA, helping stabilize their relative orientation. An extended beta-hairpin in the C-terminus forms part of the polypeptide exit tunnel, in which it helps forms a bend with protein L4, while most of the rest of the protein is located at the polypeptide exit tunnel on the outside of the subunit.

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-1462-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L22UniRule annotation
Alternative name(s):
Hl23
Hmal22
Gene namesi
Name:rpl22UniRule annotation
Ordered Locus Names:rrnAC1606
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. large ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 15515450S ribosomal protein L22PRO_0000125272Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Contacts the macrolide antibiotic tylosin in the polypeptide exit tunnel.2 PublicationsUniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC1606.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 133
Beta strandi14 – 229
Helixi26 – 3611
Helixi41 – 5212
Beta strandi61 – 633
Beta strandi74 – 807
Helixi83 – 10220
Helixi107 – 1093
Beta strandi110 – 12314
Beta strandi126 – 1283
Helixi130 – 1323
Beta strandi134 – 1363
Beta strandi139 – 14911

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFKX-ray2.40O2-155[»]
1JJ2X-ray2.40Q2-155[»]
1K73X-ray3.01S2-155[»]
1K8AX-ray3.00S2-155[»]
1K9MX-ray3.00S2-155[»]
1KC8X-ray3.01S2-155[»]
1KD1X-ray3.00S2-155[»]
1KQSX-ray3.10Q2-155[»]
1M1KX-ray3.20S2-155[»]
1M90X-ray2.80S2-155[»]
1N8RX-ray3.00S2-155[»]
1NJIX-ray3.00S2-155[»]
1Q7YX-ray3.20S2-155[»]
1Q81X-ray2.95S2-155[»]
1Q82X-ray2.98S2-155[»]
1Q86X-ray3.00S2-155[»]
1QVFX-ray3.10Q2-155[»]
1QVGX-ray2.90Q2-155[»]
1S72X-ray2.40R1-155[»]
1VQ4X-ray2.70R1-155[»]
1VQ5X-ray2.60R1-155[»]
1VQ6X-ray2.70R1-155[»]
1VQ7X-ray2.50R1-155[»]
1VQ8X-ray2.20R1-155[»]
1VQ9X-ray2.40R1-155[»]
1VQKX-ray2.30R1-155[»]
1VQLX-ray2.30R1-155[»]
1VQMX-ray2.30R1-155[»]
1VQNX-ray2.40R1-155[»]
1VQOX-ray2.20R1-155[»]
1VQPX-ray2.25R1-155[»]
1W2BX-ray3.50Q2-155[»]
1YHQX-ray2.40R1-155[»]
1YI2X-ray2.65R1-155[»]
1YIJX-ray2.60R1-155[»]
1YITX-ray2.80R1-155[»]
1YJ9X-ray2.80R1-152[»]
1YJNX-ray3.00R1-155[»]
1YJWX-ray2.90R1-155[»]
2OTJX-ray2.90R1-155[»]
2OTLX-ray2.70R1-155[»]
2QA4X-ray3.00R1-155[»]
2QEXX-ray2.90R1-155[»]
3CC2X-ray2.40R1-155[»]
3CC4X-ray2.70R1-155[»]
3CC7X-ray2.70R1-155[»]
3CCEX-ray2.75R1-155[»]
3CCJX-ray2.70R1-155[»]
3CCLX-ray2.90R1-155[»]
3CCMX-ray2.55R1-155[»]
3CCQX-ray2.90R1-155[»]
3CCRX-ray3.00R1-155[»]
3CCSX-ray2.95R1-155[»]
3CCUX-ray2.80R1-155[»]
3CCVX-ray2.90R1-155[»]
3CD6X-ray2.75R1-155[»]
3CMAX-ray2.80R1-155[»]
3CMEX-ray2.95R1-155[»]
3CPWX-ray2.70Q1-155[»]
3CXCX-ray3.00Q2-155[»]
3G4SX-ray3.20R2-151[»]
3G6EX-ray2.70R2-151[»]
3G71X-ray2.85R2-151[»]
3I55X-ray3.11R1-155[»]
3I56X-ray2.90R1-155[»]
3OW2X-ray2.70Q2-151[»]
4ADXelectron microscopy6.60R1-155[»]
4HUBX-ray2.40R1-155[»]
ProteinModelPortaliP10970.
SMRiP10970. Positions 2-151.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10970.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L22P family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0091.
HOGENOMiHOG000205045.
KOiK02890.
OMAiRFGYSFQ.

Family and domain databases

Gene3Di3.90.470.10. 1 hit.
HAMAPiMF_01331_A. Ribosomal_L22_A.
InterProiIPR001063. Ribosomal_L22.
IPR018260. Ribosomal_L22/L17_CS.
IPR005721. Ribosomal_L22/L17_euk/arc.
[Graphical view]
PANTHERiPTHR11593. PTHR11593. 1 hit.
PfamiPF00237. Ribosomal_L22. 1 hit.
[Graphical view]
SUPFAMiSSF54843. SSF54843. 1 hit.
TIGRFAMsiTIGR01038. L22_arch. 1 hit.
PROSITEiPS00464. RIBOSOMAL_L22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10970-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGISYSVEAD PDTTAKAMLR ERQMSFKHSK AIAREIKGKT AGEAVDYLEA
60 70 80 90 100
VIEGDQPVPF KQHNSGVGHK SKVDGWDAGR YPEKASKAFL DLLENAVGNA
110 120 130 140 150
DHQGFDGEAM TIKHVAAHKV GEQQGRKPRA MGRASAWNSP QVDVELILEE

PEVED
Length:155
Mass (Da):16,941
Last modified:January 23, 2007 - v2
Checksum:iE6730272296C0D7C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05222 Genomic DNA. Translation: AAA86864.1.
AY596297 Genomic DNA. Translation: AAV46523.1.
PIRiH35063. R5HS22.
RefSeqiWP_011223742.1. NC_006396.1.
YP_136229.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV46523; AAV46523; rrnAC1606.
GeneIDi3128443.
KEGGihma:rrnAC1606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05222 Genomic DNA. Translation: AAA86864.1 .
AY596297 Genomic DNA. Translation: AAV46523.1 .
PIRi H35063. R5HS22.
RefSeqi WP_011223742.1. NC_006396.1.
YP_136229.1. NC_006396.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FFK X-ray 2.40 O 2-155 [» ]
1JJ2 X-ray 2.40 Q 2-155 [» ]
1K73 X-ray 3.01 S 2-155 [» ]
1K8A X-ray 3.00 S 2-155 [» ]
1K9M X-ray 3.00 S 2-155 [» ]
1KC8 X-ray 3.01 S 2-155 [» ]
1KD1 X-ray 3.00 S 2-155 [» ]
1KQS X-ray 3.10 Q 2-155 [» ]
1M1K X-ray 3.20 S 2-155 [» ]
1M90 X-ray 2.80 S 2-155 [» ]
1N8R X-ray 3.00 S 2-155 [» ]
1NJI X-ray 3.00 S 2-155 [» ]
1Q7Y X-ray 3.20 S 2-155 [» ]
1Q81 X-ray 2.95 S 2-155 [» ]
1Q82 X-ray 2.98 S 2-155 [» ]
1Q86 X-ray 3.00 S 2-155 [» ]
1QVF X-ray 3.10 Q 2-155 [» ]
1QVG X-ray 2.90 Q 2-155 [» ]
1S72 X-ray 2.40 R 1-155 [» ]
1VQ4 X-ray 2.70 R 1-155 [» ]
1VQ5 X-ray 2.60 R 1-155 [» ]
1VQ6 X-ray 2.70 R 1-155 [» ]
1VQ7 X-ray 2.50 R 1-155 [» ]
1VQ8 X-ray 2.20 R 1-155 [» ]
1VQ9 X-ray 2.40 R 1-155 [» ]
1VQK X-ray 2.30 R 1-155 [» ]
1VQL X-ray 2.30 R 1-155 [» ]
1VQM X-ray 2.30 R 1-155 [» ]
1VQN X-ray 2.40 R 1-155 [» ]
1VQO X-ray 2.20 R 1-155 [» ]
1VQP X-ray 2.25 R 1-155 [» ]
1W2B X-ray 3.50 Q 2-155 [» ]
1YHQ X-ray 2.40 R 1-155 [» ]
1YI2 X-ray 2.65 R 1-155 [» ]
1YIJ X-ray 2.60 R 1-155 [» ]
1YIT X-ray 2.80 R 1-155 [» ]
1YJ9 X-ray 2.80 R 1-152 [» ]
1YJN X-ray 3.00 R 1-155 [» ]
1YJW X-ray 2.90 R 1-155 [» ]
2OTJ X-ray 2.90 R 1-155 [» ]
2OTL X-ray 2.70 R 1-155 [» ]
2QA4 X-ray 3.00 R 1-155 [» ]
2QEX X-ray 2.90 R 1-155 [» ]
3CC2 X-ray 2.40 R 1-155 [» ]
3CC4 X-ray 2.70 R 1-155 [» ]
3CC7 X-ray 2.70 R 1-155 [» ]
3CCE X-ray 2.75 R 1-155 [» ]
3CCJ X-ray 2.70 R 1-155 [» ]
3CCL X-ray 2.90 R 1-155 [» ]
3CCM X-ray 2.55 R 1-155 [» ]
3CCQ X-ray 2.90 R 1-155 [» ]
3CCR X-ray 3.00 R 1-155 [» ]
3CCS X-ray 2.95 R 1-155 [» ]
3CCU X-ray 2.80 R 1-155 [» ]
3CCV X-ray 2.90 R 1-155 [» ]
3CD6 X-ray 2.75 R 1-155 [» ]
3CMA X-ray 2.80 R 1-155 [» ]
3CME X-ray 2.95 R 1-155 [» ]
3CPW X-ray 2.70 Q 1-155 [» ]
3CXC X-ray 3.00 Q 2-155 [» ]
3G4S X-ray 3.20 R 2-151 [» ]
3G6E X-ray 2.70 R 2-151 [» ]
3G71 X-ray 2.85 R 2-151 [» ]
3I55 X-ray 3.11 R 1-155 [» ]
3I56 X-ray 2.90 R 1-155 [» ]
3OW2 X-ray 2.70 Q 2-151 [» ]
4ADX electron microscopy 6.60 R 1-155 [» ]
4HUB X-ray 2.40 R 1-155 [» ]
ProteinModelPortali P10970.
SMRi P10970. Positions 2-151.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272569.rrnAC1606.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV46523 ; AAV46523 ; rrnAC1606 .
GeneIDi 3128443.
KEGGi hma:rrnAC1606.

Phylogenomic databases

eggNOGi COG0091.
HOGENOMi HOG000205045.
KOi K02890.
OMAi RFGYSFQ.

Enzyme and pathway databases

BioCyci HMAR272569:GJDH-1462-MONOMER.

Miscellaneous databases

EvolutionaryTracei P10970.

Family and domain databases

Gene3Di 3.90.470.10. 1 hit.
HAMAPi MF_01331_A. Ribosomal_L22_A.
InterProi IPR001063. Ribosomal_L22.
IPR018260. Ribosomal_L22/L17_CS.
IPR005721. Ribosomal_L22/L17_euk/arc.
[Graphical view ]
PANTHERi PTHR11593. PTHR11593. 1 hit.
Pfami PF00237. Ribosomal_L22. 1 hit.
[Graphical view ]
SUPFAMi SSF54843. SSF54843. 1 hit.
TIGRFAMsi TIGR01038. L22_arch. 1 hit.
PROSITEi PS00464. RIBOSOMAL_L22. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization and nucleotide sequence of a gene cluster coding for eight ribosomal proteins in the archaebacterium Halobacterium marismortui."
    Arndt E., Kroemer W., Hatakeyama T.
    J. Biol. Chem. 265:3034-3039(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  3. "The primary structures of ribosomal proteins L16, L23 and L33 from the archaebacterium Halobacterium marismortui."
    Hatakeyama T., Hatakeyama T., Kimura M.
    FEBS Lett. 240:21-28(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-155.
  4. "Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane."
    Walsh M.J., McDougall J., Wittmann-Liebold B.
    Biochemistry 27:6867-6876(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-27.
  5. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
    Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
    Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  6. "The structural basis of ribosome activity in peptide bond synthesis."
    Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
    Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  7. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
    Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
    Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  8. "The kink-turn: a new RNA secondary structure motif."
    Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
    EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  9. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
    Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
    Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  11. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
    Hansen J.L., Moore P.B., Steitz T.A.
    J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  12. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
    Schmeing T.M., Moore P.B., Steitz T.A.
    RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
  13. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
    Gabdulkhakov A., Nikonov S., Garber M.
    Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

Entry informationi

Entry nameiRL22_HALMA
AccessioniPrimary (citable) accession number: P10970
Secondary accession number(s): Q5V1S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3