Reviewed, UniProtKB/Swiss-Prot P10969 (AGI3_WHEAT)

Names and origin

Protein names	Recommended name:     Agglutinin isolectin 3 Alternative name(s):     WGA3
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	186 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.
Subunit structure	Homodimer, u-shaped. Ref.2
Miscellaneous	The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.
Sequence similarities	Contains 4 chitin-binding type-1 domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Chitin-binding Lectin
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 171

171

Agglutinin isolectin 3

PRO_0000005259

Propeptide

172 – 186

15

PRO_0000005260

Regions

Domain

1 – 42

42

Chitin-binding type-1 1

Domain

43 – 85

43

Chitin-binding type-1 2

Domain

86 – 128

43

Chitin-binding type-1 3

Domain

129 – 171

43

Chitin-binding type-1 4

Region

10 – 12

3

Substrate binding By similarity

Region

62 – 73

12

Substrate binding

Region

114 – 115

2

Substrate binding

Amino acid modifications

Modified residue

1

1

Pyrrolidone carboxylic acid

Glycosylation

180

1

N-linked (GlcNAc...)

Disulfide bond

3 ↔ 18

Disulfide bond

12 ↔ 24

Disulfide bond

17 ↔ 31

Disulfide bond

35 ↔ 40

Disulfide bond

46 ↔ 61

Disulfide bond

55 ↔ 67

Disulfide bond

60 ↔ 74

Disulfide bond

78 ↔ 83

Disulfide bond

89 ↔ 104

Disulfide bond

98 ↔ 110

Disulfide bond

103 ↔ 117

Disulfide bond

121 ↔ 126

Disulfide bond

132 ↔ 147

Disulfide bond

141 ↔ 153

Disulfide bond

146 ↔ 160

Disulfide bond

164 ↔ 169

Experimental info

Non-terminal residue

1

1

Secondary structure

1

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186

Helix Strand Turn

Details...

Turn

4 – 8

5

Helix

13 – 15

3

Helix

28 – 31

4

Beta strand

37 – 39

3

Helix

48 – 50

3

Helix

56 – 58

3

Beta strand

65 – 68

4

Turn

71 – 73

3

Beta strand

80 – 82

3

Turn

90 – 95

6

Beta strand

108 – 111

4

Helix

114 – 117

4

Beta strand

123 – 125

3

Turn

133 – 138

6

Helix

157 – 160

4

Beta strand

166 – 168

3

Sequences

	Sequence		Length	Mass (Da)
	P10969-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 68461A20339378FD	FASTA	186	18,756
	10 20 30 40 50 60 QRCGEQGSGM ECPNNLCCSQ YGYCGMGGDY CGKGCQNGAC WTSKRCGSQA GGKTCPNNHC 70 80 90 100 110 120 CSQYGHCGFG AEYCGAGCQG GPCRADIKCG SQAGGKLCPN NLCCSQWGYC GLGSEFCGEG 130 140 150 160 170 180 CQNGACSTDK PCGKDAGGRV CTNNYCCSKW GSCGIGPGYC GAGCQSGGCD GVFAEAIATN STLLAE

References

[1]	"Isolation and characterization of a cDNA clone encoding wheat germ agglutinin." Raikhel N.V., Wilkins T.A. Proc. Natl. Acad. Sci. U.S.A. 84:6745-6749(1987) [PubMed: 16578818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"X-ray structure of wheat germ agglutinin isolectin 3." Harata K., Nagahora H., Jigami Y. Acta Crystallogr. D 51:1013-1019(1995) [PubMed: 15299769] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT.
[3]	"Interactions of wheat-germ agglutinin with GlcNAc beta 1,6Gal sequence." Muraki M., Ishimura M., Harata K. Biochim. Biophys. Acta 1569:10-20(2002) [PubMed: 11853952] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH SUBSTRATES.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J02961 mRNA. Translation: AAA34257.1.

PIR

A28401.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K7T	X-ray	2.40	A/B	2-186	[»]
1K7U	X-ray	2.20	A/B	2-186	[»]
1K7V	X-ray	2.20	A/B	2-186	[»]
1WGT	X-ray	1.90	A/B	2-186	[»]
2UWZ	X-ray	1.70	A/B	2-171	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM18. Carbohydrate-Binding Module Family 18.

Organism-specific databases

Gramene

P10969.

Family and domain databases

InterPro

IPR018371. Chitin-binding_1_CS.
IPR001002. Chitin_bd_1.
IPR000726. Glyco_hydro_19_cat.
[Graphical view]

Gene3D

G3DSA:3.30.60.10. Chitin_bd_1. 4 hits.

PANTHER

PTHR22595. Glyco_hydro_19_cat. 1 hit.

Pfam

PF00187. Chitin_bind_1. 4 hits.
[Graphical view]

PRINTS

PR00451. CHITINBINDNG.

ProDom

PD000609. Chitin_binding_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00270. ChtBD1. 4 hits.
[Graphical view]

PROSITE

PS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AGI3_WHEAT

Accession

Primary (citable) accession number: P10969

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)