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P10969

- AGI3_WHEAT

UniProt

P10969 - AGI3_WHEAT

Protein

Agglutinin isolectin 3

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

    GO - Molecular functioni

    1. chitin binding Source: UniProtKB-KW

    Keywords - Ligandi

    Chitin-binding, Lectin

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Agglutinin isolectin 3
    Alternative name(s):
    WGA3
    OrganismiTriticum aestivum (Wheat)
    Taxonomic identifieri4565 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
    ProteomesiUP000019116: Unplaced

    Organism-specific databases

    GrameneiP10969.

    Pathology & Biotechi

    Protein family/group databases

    Allergomei650. Tri a 18.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 171171Agglutinin isolectin 3PRO_0000005259Add
    BLAST
    Propeptidei172 – 18615PRO_0000005260Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid
    Disulfide bondi3 ↔ 18
    Disulfide bondi12 ↔ 24
    Disulfide bondi17 ↔ 31
    Disulfide bondi35 ↔ 40
    Disulfide bondi46 ↔ 61
    Disulfide bondi55 ↔ 67
    Disulfide bondi60 ↔ 74
    Disulfide bondi78 ↔ 83
    Disulfide bondi89 ↔ 104
    Disulfide bondi98 ↔ 110
    Disulfide bondi103 ↔ 117
    Disulfide bondi121 ↔ 126
    Disulfide bondi132 ↔ 147
    Disulfide bondi141 ↔ 153
    Disulfide bondi146 ↔ 160
    Disulfide bondi164 ↔ 169
    Glycosylationi180 – 1801N-linked (GlcNAc...)

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Homodimer, u-shaped.2 Publications

    Structurei

    Secondary structure

    1
    186
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Turni7 – 93
    Helixi13 – 153
    Beta strandi24 – 274
    Helixi28 – 314
    Beta strandi32 – 343
    Beta strandi37 – 393
    Helixi48 – 503
    Helixi56 – 583
    Beta strandi65 – 706
    Helixi71 – 744
    Beta strandi80 – 823
    Helixi90 – 934
    Helixi99 – 1013
    Beta strandi108 – 1136
    Helixi114 – 1174
    Beta strandi123 – 1253
    Helixi133 – 1353
    Turni136 – 1383
    Helixi142 – 1443
    Beta strandi153 – 1564
    Helixi157 – 1604
    Beta strandi166 – 1683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K7TX-ray2.40A/B1-186[»]
    1K7UX-ray2.20A/B1-186[»]
    1K7VX-ray2.20A/B2-186[»]
    1WGTX-ray1.90A/B2-186[»]
    2X52X-ray1.70A/B2-171[»]
    ProteinModelPortaliP10969.
    SMRiP10969. Positions 2-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10969.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 4242Chitin-binding type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini43 – 8543Chitin-binding type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini86 – 12843Chitin-binding type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini129 – 17143Chitin-binding type-1 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 123Substrate bindingBy similarity
    Regioni62 – 7312Substrate bindingAdd
    BLAST
    Regioni114 – 1152Substrate binding

    Sequence similaritiesi

    Contains 4 chitin-binding type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di3.30.60.10. 4 hits.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 4 hits.
    [Graphical view]
    PRINTSiPR00451. CHITINBINDNG.
    ProDomiPD000609. Chitin_bd_1. 3 hits.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 4 hits.
    [Graphical view]
    SUPFAMiSSF57016. SSF57016. 4 hits.
    PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
    PS50941. CHIT_BIND_I_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10969-1 [UniParc]FASTAAdd to Basket

    « Hide

    QRCGEQGSGM ECPNNLCCSQ YGYCGMGGDY CGKGCQNGAC WTSKRCGSQA    50
    GGKTCPNNHC CSQYGHCGFG AEYCGAGCQG GPCRADIKCG SQAGGKLCPN 100
    NLCCSQWGYC GLGSEFCGEG CQNGACSTDK PCGKDAGGRV CTNNYCCSKW 150
    GSCGIGPGYC GAGCQSGGCD GVFAEAIATN STLLAE 186
    Length:186
    Mass (Da):18,756
    Last modified:July 1, 1989 - v1
    Checksum:i68461A20339378FD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02961 mRNA. Translation: AAA34257.1.
    PIRiA28401.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02961 mRNA. Translation: AAA34257.1 .
    PIRi A28401.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K7T X-ray 2.40 A/B 1-186 [» ]
    1K7U X-ray 2.20 A/B 1-186 [» ]
    1K7V X-ray 2.20 A/B 2-186 [» ]
    1WGT X-ray 1.90 A/B 2-186 [» ]
    2X52 X-ray 1.70 A/B 2-171 [» ]
    ProteinModelPortali P10969.
    SMRi P10969. Positions 2-171.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 650. Tri a 18.
    CAZyi CBM18. Carbohydrate-Binding Module Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P10969.

    Miscellaneous databases

    EvolutionaryTracei P10969.
    PROi P10969.

    Family and domain databases

    Gene3Di 3.30.60.10. 4 hits.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 4 hits.
    [Graphical view ]
    PRINTSi PR00451. CHITINBINDNG.
    ProDomi PD000609. Chitin_bd_1. 3 hits.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 4 hits.
    [Graphical view ]
    SUPFAMi SSF57016. SSF57016. 4 hits.
    PROSITEi PS00026. CHIT_BIND_I_1. 4 hits.
    PS50941. CHIT_BIND_I_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA clone encoding wheat germ agglutinin."
      Raikhel N.V., Wilkins T.A.
      Proc. Natl. Acad. Sci. U.S.A. 84:6745-6749(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "X-ray structure of wheat germ agglutinin isolectin 3."
      Harata K., Nagahora H., Jigami Y.
      Acta Crystallogr. D 51:1013-1019(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT.
    3. "Interactions of wheat-germ agglutinin with GlcNAc beta 1,6Gal sequence."
      Muraki M., Ishimura M., Harata K.
      Biochim. Biophys. Acta 1569:10-20(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH SUBSTRATES.

    Entry informationi

    Entry nameiAGI3_WHEAT
    AccessioniPrimary (citable) accession number: P10969
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3