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P10968

- AGI1_WHEAT

UniProt

P10968 - AGI1_WHEAT

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Protein

Agglutinin isolectin 1

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. chitin binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Chitin-binding, Lectin

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Agglutinin isolectin 1
Alternative name(s):
Isolectin A
WGA1
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
ProteomesiUP000019116: Unplaced

Organism-specific databases

GrameneiP10968.

Pathology & Biotechi

Protein family/group databases

Allergomei3501. Tri a 18.0101.
650. Tri a 18.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 197171Agglutinin isolectin 1PRO_0000005255Add
BLAST
Propeptidei198 – 21215PRO_0000005256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Pyrrolidone carboxylic acid1 Publication
Disulfide bondi29 ↔ 44
Disulfide bondi38 ↔ 50
Disulfide bondi43 ↔ 57
Disulfide bondi61 ↔ 66
Disulfide bondi72 ↔ 87
Disulfide bondi81 ↔ 93
Disulfide bondi86 ↔ 100
Disulfide bondi104 ↔ 109
Disulfide bondi115 ↔ 130
Disulfide bondi124 ↔ 136
Disulfide bondi129 ↔ 143
Disulfide bondi147 ↔ 152
Disulfide bondi158 ↔ 173
Disulfide bondi167 ↔ 179
Disulfide bondi172 ↔ 186
Disulfide bondi190 ↔ 195

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer, u-shaped.1 Publication

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 334Combined sources
Helixi39 – 413Combined sources
Beta strandi48 – 536Combined sources
Helixi54 – 574Combined sources
Beta strandi63 – 653Combined sources
Helixi73 – 764Combined sources
Helixi82 – 843Combined sources
Beta strandi91 – 966Combined sources
Helixi97 – 1004Combined sources
Beta strandi106 – 1083Combined sources
Helixi116 – 1194Combined sources
Helixi125 – 1273Combined sources
Beta strandi134 – 1396Combined sources
Helixi140 – 1434Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi149 – 1513Combined sources
Turni160 – 1645Combined sources
Helixi168 – 1703Combined sources
Beta strandi179 – 1824Combined sources
Helixi183 – 1864Combined sources
Beta strandi192 – 1943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WGCX-ray2.20A/B28-197[»]
2CWGX-ray2.00A/B28-197[»]
2UVOX-ray1.40A/B/E/F28-197[»]
2X3TX-ray2.75A/B/C/D28-197[»]
4AMLX-ray1.60A/B28-197[»]
7WGAX-ray2.00A/B28-197[»]
ProteinModelPortaliP10968.
SMRiP10968. Positions 27-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10968.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6842Chitin-binding type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini69 – 11143Chitin-binding type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini112 – 15443Chitin-binding type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini155 – 19743Chitin-binding type-1 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 383Substrate binding
Regioni88 – 9912Substrate bindingAdd
BLAST
Regioni140 – 1412Substrate binding

Sequence similaritiesi

Contains 4 chitin-binding type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10968-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMMSTRALA LGAAAVLAFA AATAQAQRCG EQGSNMECPN NLCCSQYGYC
60 70 80 90 100
GMGGDYCGKG CQNGACWTSK RCGSQAGGAT CTNNQCCSQY GYCGFGAEYC
110 120 130 140 150
GAGCQGGPCR ADIKCGSQAG GKLCPNNLCC SQWGFCGLGS EFCGGGCQSG
160 170 180 190 200
ACSTDKPCGK DAGGRVCTNN YCCSKWGSCG IGPGYCGAGC QSGGCDGVFA
210
EAITANSTLL QE
Length:212
Mass (Da):21,239
Last modified:November 1, 1990 - v2
Checksum:iEC7B6F007DDC15EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631N → D AA sequence (PubMed:2499688)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25536 mRNA. Translation: AAA34256.1.
PIRiS09623.
UniGeneiTa.12645.

Genome annotation databases

EnsemblPlantsiTraes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25536 mRNA. Translation: AAA34256.1 .
PIRi S09623.
UniGenei Ta.12645.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WGC X-ray 2.20 A/B 28-197 [» ]
2CWG X-ray 2.00 A/B 28-197 [» ]
2UVO X-ray 1.40 A/B/E/F 28-197 [» ]
2X3T X-ray 2.75 A/B/C/D 28-197 [» ]
4AML X-ray 1.60 A/B 28-197 [» ]
7WGA X-ray 2.00 A/B 28-197 [» ]
ProteinModelPortali P10968.
SMRi P10968. Positions 27-197.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3501. Tri a 18.0101.
650. Tri a 18.
CAZyi CBM18. Carbohydrate-Binding Module Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C .

Organism-specific databases

Gramenei P10968.

Miscellaneous databases

EvolutionaryTracei P10968.
PROi P10968.

Family and domain databases

Gene3Di 3.30.60.10. 4 hits.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 4 hits.
[Graphical view ]
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 3 hits.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 4 hits.
[Graphical view ]
SUPFAMi SSF57016. SSF57016. 4 hits.
PROSITEi PS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of cDNA clones encoding wheat germ agglutinin isolectins A and D."
    Smith J.J., Raikhel N.V.
    Plant Mol. Biol. 13:601-603(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat."
    Wright C.S., Raikhel N.V.
    J. Mol. Evol. 28:327-336(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-197.
    Tissue: Germ.
  3. "Comparison of the refined crystal structures of two wheat germ isolectins."
    Wright C.S.
    J. Mol. Biol. 209:475-487(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-197, PYROGLUTAMATE FORMATION AT GLN-27.
  4. "2.2-A resolution structure analysis of two refined N-acetylneuraminyl-lactose-wheat germ agglutinin isolectin complexes."
    Wright C.S.
    J. Mol. Biol. 215:635-651(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH N-ACETYLNEURAMINYL-LACTOSE.

Entry informationi

Entry nameiAGI1_WHEAT
AccessioniPrimary (citable) accession number: P10968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: November 26, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3