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Protein

Agglutinin isolectin 1

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Chitin-binding, Lectin

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Agglutinin isolectin 1
Alternative name(s):
Isolectin A
WGA1
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Pathology & Biotechi

Protein family/group databases

Allergomei3501. Tri a 18.0101.
650. Tri a 18.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000000525527 – 197Agglutinin isolectin 1Add BLAST171
PropeptideiPRO_0000005256198 – 212Add BLAST15

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi29 ↔ 44
Disulfide bondi38 ↔ 50
Disulfide bondi43 ↔ 57
Disulfide bondi61 ↔ 66
Disulfide bondi72 ↔ 87
Disulfide bondi81 ↔ 93
Disulfide bondi86 ↔ 100
Disulfide bondi104 ↔ 109
Disulfide bondi115 ↔ 130
Disulfide bondi124 ↔ 136
Disulfide bondi129 ↔ 143
Disulfide bondi147 ↔ 152
Disulfide bondi158 ↔ 173
Disulfide bondi167 ↔ 179
Disulfide bondi172 ↔ 186
Disulfide bondi190 ↔ 195

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer, u-shaped.1 Publication

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 33Combined sources4
Helixi39 – 41Combined sources3
Beta strandi48 – 53Combined sources6
Helixi54 – 57Combined sources4
Beta strandi63 – 65Combined sources3
Helixi73 – 76Combined sources4
Helixi82 – 84Combined sources3
Beta strandi91 – 96Combined sources6
Helixi97 – 100Combined sources4
Beta strandi106 – 108Combined sources3
Helixi116 – 119Combined sources4
Helixi125 – 127Combined sources3
Beta strandi134 – 139Combined sources6
Helixi140 – 143Combined sources4
Beta strandi144 – 146Combined sources3
Beta strandi149 – 151Combined sources3
Turni160 – 164Combined sources5
Helixi168 – 170Combined sources3
Beta strandi179 – 182Combined sources4
Helixi183 – 186Combined sources4
Beta strandi192 – 194Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGCX-ray2.20A/B28-197[»]
2CWGX-ray2.00A/B28-197[»]
2UVOX-ray1.40A/B/E/F28-197[»]
2X3TX-ray2.75A/B/C/D28-197[»]
4AMLX-ray1.60A/B28-197[»]
7WGAX-ray2.00A/B28-197[»]
ProteinModelPortaliP10968.
SMRiP10968.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10968.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 68Chitin-binding type-1 1PROSITE-ProRule annotationAdd BLAST42
Domaini69 – 111Chitin-binding type-1 2PROSITE-ProRule annotationAdd BLAST43
Domaini112 – 154Chitin-binding type-1 3PROSITE-ProRule annotationAdd BLAST43
Domaini155 – 197Chitin-binding type-1 4PROSITE-ProRule annotationAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 38Substrate binding3
Regioni88 – 99Substrate bindingAdd BLAST12
Regioni140 – 141Substrate binding2

Sequence similaritiesi

Contains 4 chitin-binding type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

OMAiKCGSNAN.
OrthoDBiEOG09360PEG.

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMMSTRALA LGAAAVLAFA AATAQAQRCG EQGSNMECPN NLCCSQYGYC
60 70 80 90 100
GMGGDYCGKG CQNGACWTSK RCGSQAGGAT CTNNQCCSQY GYCGFGAEYC
110 120 130 140 150
GAGCQGGPCR ADIKCGSQAG GKLCPNNLCC SQWGFCGLGS EFCGGGCQSG
160 170 180 190 200
ACSTDKPCGK DAGGRVCTNN YCCSKWGSCG IGPGYCGAGC QSGGCDGVFA
210
EAITANSTLL QE
Length:212
Mass (Da):21,239
Last modified:November 1, 1990 - v2
Checksum:iEC7B6F007DDC15EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63N → D AA sequence (PubMed:2499688).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25536 mRNA. Translation: AAA34256.1.
PIRiS09623.
UniGeneiTa.12645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25536 mRNA. Translation: AAA34256.1.
PIRiS09623.
UniGeneiTa.12645.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGCX-ray2.20A/B28-197[»]
2CWGX-ray2.00A/B28-197[»]
2UVOX-ray1.40A/B/E/F28-197[»]
2X3TX-ray2.75A/B/C/D28-197[»]
4AMLX-ray1.60A/B28-197[»]
7WGAX-ray2.00A/B28-197[»]
ProteinModelPortaliP10968.
SMRiP10968.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3501. Tri a 18.0101.
650. Tri a 18.
CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OMAiKCGSNAN.
OrthoDBiEOG09360PEG.

Miscellaneous databases

EvolutionaryTraceiP10968.
PROiP10968.

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGI1_WHEAT
AccessioniPrimary (citable) accession number: P10968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.