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P10968

- AGI1_WHEAT

UniProt

P10968 - AGI1_WHEAT

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Protein

Agglutinin isolectin 1

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Chitin-binding, Lectin

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Agglutinin isolectin 1
Alternative name(s):
Isolectin A
WGA1
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
ProteomesiUP000019116: Unplaced

Organism-specific databases

GrameneiP10968.

Pathology & Biotechi

Protein family/group databases

Allergomei3501. Tri a 18.0101.
650. Tri a 18.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 197171Agglutinin isolectin 1PRO_0000005255Add
BLAST
Propeptidei198 – 21215PRO_0000005256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Pyrrolidone carboxylic acid
Disulfide bondi29 ↔ 44
Disulfide bondi38 ↔ 50
Disulfide bondi43 ↔ 57
Disulfide bondi61 ↔ 66
Disulfide bondi72 ↔ 87
Disulfide bondi81 ↔ 93
Disulfide bondi86 ↔ 100
Disulfide bondi104 ↔ 109
Disulfide bondi115 ↔ 130
Disulfide bondi124 ↔ 136
Disulfide bondi129 ↔ 143
Disulfide bondi147 ↔ 152
Disulfide bondi158 ↔ 173
Disulfide bondi167 ↔ 179
Disulfide bondi172 ↔ 186
Disulfide bondi190 ↔ 195

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer, u-shaped.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 334
Helixi39 – 413
Beta strandi48 – 536
Helixi54 – 574
Beta strandi63 – 653
Helixi73 – 764
Helixi82 – 843
Beta strandi91 – 966
Helixi97 – 1004
Beta strandi106 – 1083
Helixi116 – 1194
Helixi125 – 1273
Beta strandi134 – 1396
Helixi140 – 1434
Beta strandi144 – 1463
Beta strandi149 – 1513
Turni160 – 1645
Helixi168 – 1703
Beta strandi179 – 1824
Helixi183 – 1864
Beta strandi192 – 1943

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WGCX-ray2.20A/B28-197[»]
2CWGX-ray2.00A/B28-197[»]
2UVOX-ray1.40A/B/E/F28-197[»]
2X3TX-ray2.75A/B/C/D28-197[»]
4AMLX-ray1.60A/B28-197[»]
7WGAX-ray2.00A/B28-197[»]
ProteinModelPortaliP10968.
SMRiP10968. Positions 27-197.

Miscellaneous databases

EvolutionaryTraceiP10968.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6842Chitin-binding type-1 1Add
BLAST
Domaini69 – 11143Chitin-binding type-1 2Add
BLAST
Domaini112 – 15443Chitin-binding type-1 3Add
BLAST
Domaini155 – 19743Chitin-binding type-1 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 383Substrate binding
Regioni88 – 9912Substrate bindingAdd
BLAST
Regioni140 – 1412Substrate binding

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.60.10. 4 hits.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 4 hits.
[Graphical view]
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 4 hits.
[Graphical view]
SUPFAMiSSF57016. SSF57016. 4 hits.
PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10968-1 [UniParc]FASTAAdd to Basket

« Hide

MKMMSTRALA LGAAAVLAFA AATAQAQRCG EQGSNMECPN NLCCSQYGYC    50
GMGGDYCGKG CQNGACWTSK RCGSQAGGAT CTNNQCCSQY GYCGFGAEYC 100
GAGCQGGPCR ADIKCGSQAG GKLCPNNLCC SQWGFCGLGS EFCGGGCQSG 150
ACSTDKPCGK DAGGRVCTNN YCCSKWGSCG IGPGYCGAGC QSGGCDGVFA 200
EAITANSTLL QE 212
Length:212
Mass (Da):21,239
Last modified:November 1, 1990 - v2
Checksum:iEC7B6F007DDC15EB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631N → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25536 mRNA. Translation: AAA34256.1.
PIRiS09623.
UniGeneiTa.12645.

Genome annotation databases

EnsemblPlantsiTraes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25536 mRNA. Translation: AAA34256.1 .
PIRi S09623.
UniGenei Ta.12645.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WGC X-ray 2.20 A/B 28-197 [» ]
2CWG X-ray 2.00 A/B 28-197 [» ]
2UVO X-ray 1.40 A/B/E/F 28-197 [» ]
2X3T X-ray 2.75 A/B/C/D 28-197 [» ]
4AML X-ray 1.60 A/B 28-197 [» ]
7WGA X-ray 2.00 A/B 28-197 [» ]
ProteinModelPortali P10968.
SMRi P10968. Positions 27-197.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3501. Tri a 18.0101.
650. Tri a 18.
CAZyi CBM18. Carbohydrate-Binding Module Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C .

Organism-specific databases

Gramenei P10968.

Miscellaneous databases

EvolutionaryTracei P10968.
PROi P10968.

Family and domain databases

Gene3Di 3.30.60.10. 4 hits.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 4 hits.
[Graphical view ]
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 3 hits.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 4 hits.
[Graphical view ]
SUPFAMi SSF57016. SSF57016. 4 hits.
PROSITEi PS00026. CHIT_BIND_I_1. 4 hits.
PS50941. CHIT_BIND_I_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of cDNA clones encoding wheat germ agglutinin isolectins A and D."
    Smith J.J., Raikhel N.V.
    Plant Mol. Biol. 13:601-603(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat."
    Wright C.S., Raikhel N.V.
    J. Mol. Evol. 28:327-336(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-197.
    Tissue: Germ.
  3. "Comparison of the refined crystal structures of two wheat germ isolectins."
    Wright C.S.
    J. Mol. Biol. 209:475-487(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-197, PYROGLUTAMATE FORMATION AT GLN-27.
  4. "2.2-A resolution structure analysis of two refined N-acetylneuraminyl-lactose-wheat germ agglutinin isolectin complexes."
    Wright C.S.
    J. Mol. Biol. 215:635-651(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH N-ACETYLNEURAMINYL-LACTOSE.

Entry informationi

Entry nameiAGI1_WHEAT
AccessioniPrimary (citable) accession number: P10968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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