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P10968

- AGI1_WHEAT

UniProt

P10968 - AGI1_WHEAT

Protein

Agglutinin isolectin 1

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.

    GO - Molecular functioni

    1. chitin binding Source: UniProtKB-KW

    Keywords - Ligandi

    Chitin-binding, Lectin

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Agglutinin isolectin 1
    Alternative name(s):
    Isolectin A
    WGA1
    OrganismiTriticum aestivum (Wheat)
    Taxonomic identifieri4565 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
    ProteomesiUP000019116: Unplaced

    Organism-specific databases

    GrameneiP10968.

    Pathology & Biotechi

    Protein family/group databases

    Allergomei3501. Tri a 18.0101.
    650. Tri a 18.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 197171Agglutinin isolectin 1PRO_0000005255Add
    BLAST
    Propeptidei198 – 21215PRO_0000005256Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi29 ↔ 44
    Disulfide bondi38 ↔ 50
    Disulfide bondi43 ↔ 57
    Disulfide bondi61 ↔ 66
    Disulfide bondi72 ↔ 87
    Disulfide bondi81 ↔ 93
    Disulfide bondi86 ↔ 100
    Disulfide bondi104 ↔ 109
    Disulfide bondi115 ↔ 130
    Disulfide bondi124 ↔ 136
    Disulfide bondi129 ↔ 143
    Disulfide bondi147 ↔ 152
    Disulfide bondi158 ↔ 173
    Disulfide bondi167 ↔ 179
    Disulfide bondi172 ↔ 186
    Disulfide bondi190 ↔ 195

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Homodimer, u-shaped.1 Publication

    Structurei

    Secondary structure

    1
    212
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 334
    Helixi39 – 413
    Beta strandi48 – 536
    Helixi54 – 574
    Beta strandi63 – 653
    Helixi73 – 764
    Helixi82 – 843
    Beta strandi91 – 966
    Helixi97 – 1004
    Beta strandi106 – 1083
    Helixi116 – 1194
    Helixi125 – 1273
    Beta strandi134 – 1396
    Helixi140 – 1434
    Beta strandi144 – 1463
    Beta strandi149 – 1513
    Turni160 – 1645
    Helixi168 – 1703
    Beta strandi179 – 1824
    Helixi183 – 1864
    Beta strandi192 – 1943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WGCX-ray2.20A/B28-197[»]
    2CWGX-ray2.00A/B28-197[»]
    2UVOX-ray1.40A/B/E/F28-197[»]
    2X3TX-ray2.75A/B/C/D28-197[»]
    4AMLX-ray1.60A/B28-197[»]
    7WGAX-ray2.00A/B28-197[»]
    ProteinModelPortaliP10968.
    SMRiP10968. Positions 27-197.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10968.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 6842Chitin-binding type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini69 – 11143Chitin-binding type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini112 – 15443Chitin-binding type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini155 – 19743Chitin-binding type-1 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 383Substrate binding
    Regioni88 – 9912Substrate bindingAdd
    BLAST
    Regioni140 – 1412Substrate binding

    Sequence similaritiesi

    Contains 4 chitin-binding type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di3.30.60.10. 4 hits.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 4 hits.
    [Graphical view]
    PRINTSiPR00451. CHITINBINDNG.
    ProDomiPD000609. Chitin_bd_1. 3 hits.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 4 hits.
    [Graphical view]
    SUPFAMiSSF57016. SSF57016. 4 hits.
    PROSITEiPS00026. CHIT_BIND_I_1. 4 hits.
    PS50941. CHIT_BIND_I_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10968-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMMSTRALA LGAAAVLAFA AATAQAQRCG EQGSNMECPN NLCCSQYGYC    50
    GMGGDYCGKG CQNGACWTSK RCGSQAGGAT CTNNQCCSQY GYCGFGAEYC 100
    GAGCQGGPCR ADIKCGSQAG GKLCPNNLCC SQWGFCGLGS EFCGGGCQSG 150
    ACSTDKPCGK DAGGRVCTNN YCCSKWGSCG IGPGYCGAGC QSGGCDGVFA 200
    EAITANSTLL QE 212
    Length:212
    Mass (Da):21,239
    Last modified:November 1, 1990 - v2
    Checksum:iEC7B6F007DDC15EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631N → D AA sequence (PubMed:2499688)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25536 mRNA. Translation: AAA34256.1.
    PIRiS09623.
    UniGeneiTa.12645.

    Genome annotation databases

    EnsemblPlantsiTraes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.1; Traes_1AL_B8B0A092C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25536 mRNA. Translation: AAA34256.1 .
    PIRi S09623.
    UniGenei Ta.12645.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WGC X-ray 2.20 A/B 28-197 [» ]
    2CWG X-ray 2.00 A/B 28-197 [» ]
    2UVO X-ray 1.40 A/B/E/F 28-197 [» ]
    2X3T X-ray 2.75 A/B/C/D 28-197 [» ]
    4AML X-ray 1.60 A/B 28-197 [» ]
    7WGA X-ray 2.00 A/B 28-197 [» ]
    ProteinModelPortali P10968.
    SMRi P10968. Positions 27-197.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3501. Tri a 18.0101.
    650. Tri a 18.
    CAZyi CBM18. Carbohydrate-Binding Module Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C.1 ; Traes_1AL_B8B0A092C .

    Organism-specific databases

    Gramenei P10968.

    Miscellaneous databases

    EvolutionaryTracei P10968.
    PROi P10968.

    Family and domain databases

    Gene3Di 3.30.60.10. 4 hits.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 4 hits.
    [Graphical view ]
    PRINTSi PR00451. CHITINBINDNG.
    ProDomi PD000609. Chitin_bd_1. 3 hits.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 4 hits.
    [Graphical view ]
    SUPFAMi SSF57016. SSF57016. 4 hits.
    PROSITEi PS00026. CHIT_BIND_I_1. 4 hits.
    PS50941. CHIT_BIND_I_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of cDNA clones encoding wheat germ agglutinin isolectins A and D."
      Smith J.J., Raikhel N.V.
      Plant Mol. Biol. 13:601-603(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat."
      Wright C.S., Raikhel N.V.
      J. Mol. Evol. 28:327-336(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-197.
      Tissue: Germ.
    3. "Comparison of the refined crystal structures of two wheat germ isolectins."
      Wright C.S.
      J. Mol. Biol. 209:475-487(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-197, PYROGLUTAMATE FORMATION AT GLN-27.
    4. "2.2-A resolution structure analysis of two refined N-acetylneuraminyl-lactose-wheat germ agglutinin isolectin complexes."
      Wright C.S.
      J. Mol. Biol. 215:635-651(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH N-ACETYLNEURAMINYL-LACTOSE.

    Entry informationi

    Entry nameiAGI1_WHEAT
    AccessioniPrimary (citable) accession number: P10968
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The 4 sites proposed for binding to carbohydrates (N-acetyl-D-glucosamine) of receptor molecules are on the surface of the agglutinin molecule.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3