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Reviewed, UniProtKB/Swiss-Prot P10966 (CD8B_HUMAN)

Last modified January 19, 2010. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    T-cell surface glycoprotein CD8 beta chain
Alternative name(s):
    CD_antigen=CD8b
Gene names
Name: CD8B
Synonyms: CD8B1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Subunit structure

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Isoform 3: Secreted Probable.

Post-translational modification

Phosphorylated as a consequence of T-cell activation Potential.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P10966-1)

Also known as: M-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P10966-2)

Also known as: M-3;

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: FYK → LRLHPLEKCSRMDY
Isoform 3 (identifier: P10966-3)

Also known as: S-1;

The sequence of this isoform differs from the canonical sequence as follows:
     166-195: Missing.
     208-210: FYK → PQGEGISGTFVPQCLHGYYSNTTTSQKLLNPWILKT
Isoform 4 (identifier: P10966-4)

Also known as: M-2;

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: FYK → KFNIVCLKISGFTTCCCFQILQISREYGFGVLLQKDIGQ
Isoform 5 (identifier: P10966-5)

The sequence of this isoform differs from the canonical sequence as follows:
     15-15: V → G
     16-195: Missing.
Isoform 6 (identifier: P10966-6)

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: FYK → PQGEGISGTFVPQCLHGYYSNTTTSQKLLNPWILKT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflict2131I → V in AAI00915. Ref.7

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 210189T-cell surface glycoprotein CD8 beta chain
PRO_0000014643

Regions

Topological domain22 – 170149Extracellular Potential
Transmembrane171 – 19121 Potential
Topological domain192 – 21019Cytoplasmic Potential
Domain22 – 132111Ig-like V-type

Amino acid modifications

Modified residue1301Phosphothreonine By similarity
Modified residue2091Phosphotyrosine Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 116 Potential

Natural variations

Alternative sequence151V → G in isoform 5.
VSP_012007
Alternative sequence16 – 195180Missing in isoform 5.
VSP_012008
Alternative sequence166 – 19530Missing in isoform 3.
VSP_002492
Alternative sequence208 – 2103FYK → LRLHPLEKCSRMDY in isoform 2.
VSP_002490
Alternative sequence208 – 2103FYK → PQGEGISGTFVPQCLHGYYS NTTTSQKLLNPWILKT in isoform 3 and isoform 6.
VSP_002493
Alternative sequence208 – 2103FYK → KFNIVCLKISGFTTCCCFQI LQISREYGFGVLLQKDIGQ in isoform 4.
VSP_002491

Experimental info

Sequence conflict1801V → I in AAI00915. Ref.7

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (M-1) [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 675AD919585F4B80

FASTA21023,722
        10         20         30         40         50         60 
MRPRLWLLLA AQLTVLHGNS VLQQTPAYIK VQTNKMVMLS CEAKISLSNM RIYWLRQRQA 

        70         80         90        100        110        120 
PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI LNLTSVKPED SGIYFCMIVG 

       130        140        150        160        170        180 
SPELTFGKGT QLSVVDFLPT TAQPTKKSTL KKRVCRLPRP ETQKGPLCSP ITLGLLVAGV 

       190        200        210 
LVLLVSLGVA IHLCCRRRRA RLRFMKQFYK

« Hide

Isoform 2 (M-3).

Checksum: F5D8E834AE123671
Show »

FASTA22125,027
Isoform 3 (S-1).

Checksum: 79F81C2F066900D3
Show »

FASTA21324,276
Isoform 4 (M-2).

Checksum: 33E6112A23CE59CD
Show »

FASTA24627,681
Isoform 5.

Checksum: AD88E7EF2EDF87D1
Show »

FASTA303,834
Isoform 6.

Checksum: 9D6B283609CC0752
Show »

FASTA24327,246

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References

« Hide 'large scale' references
[1]"A second subunit of CD8 is expressed in human T cells."
Norment A.M., Littman D.R.
EMBO J. 7:3433-3439(1988) [PubMed: 3145195] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: T-cell.
[2]"The human Lyt-3 molecule requires CD8 for cell surface expression."
Disanto J.P., Knowles R.W., Flomenberg N.
EMBO J. 7:3465-3470(1988) [PubMed: 3145196] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"A second chain of human CD8 is expressed on peripheral blood lymphocytes."
Shiue L., Gorman S.D., Parnes J.R.
J. Exp. Med. 168:1993-2005(1988) [PubMed: 3264320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Recent duplication of the two human CD8 beta-chain genes."
Nakayama K., Kawachi Y., Tokito S., Minami N., Yamamoto R., Imai T., Gachelin G., Nakauchi H.
J. Immunol. 148:1919-1927(1992) [PubMed: 1541829] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2; 3 AND 4).
[5]"Transcriptional diversity at the duplicated human CD8 beta loci."
DiSanto J.P., Smith D., de Bruin D., Lacy E., Flomenberg N.
Eur. J. Immunol. 23:320-326(1993) [PubMed: 8436166] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 5), ALTERNATIVE SPLICING.
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6).
+Additional computationally mapped references.

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Web resources

Wikipedia

CD8 entry

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X13444 mRNA. Translation: CAA31795.1.
X13445 mRNA. Translation: CAA31796.1.
X13446 mRNA. Translation: CAA31797.1.
X13452 mRNA. Translation: CAA31803.1.
Y00805 mRNA. Translation: CAA68750.1.
M36712 mRNA. Translation: AAA35664.1.
S87090 expand/collapse EMBL AC list , S87068, S87070, S87073, S87081 Genomic DNA. Translation: AAB21669.2.
S87087 expand/collapse EMBL AC list , S87068, S87070, S87073, S87078, S87081 Genomic DNA. Translation: AAB21670.2.
S87083 expand/collapse EMBL AC list , S87068, S87070, S87073, S87078, S87081 Genomic DNA. Translation: AAB21671.2.
S87083 expand/collapse EMBL AC list , S87068, S87070, S87073, S87078, S87081 Genomic DNA. Translation: AAB21672.2.
S55731, S55730, S55733 Genomic DNA. Translation: AAD13877.1.
AC111200 Genomic DNA. No translation available.
BC100911 mRNA. Translation: AAI00912.1.
BC100913 mRNA. Translation: AAI00914.1.
BC100914 mRNA. Translation: AAI00915.1.
IPIIPI00023641.
IPI00218643.
IPI00218644.
IPI00218645.
IPI00333756.
IPI00470704.
PIRD46482.
E46482. S01647.
C46482. S01873.
B46482. S01874.
T01073.
RefSeqNP_004922.1.
NP_742097.1.
NP_742099.1.
NP_742100.1.
NP_757362.1.
UniGeneHs.405667

3D structure databases

SMRP10966. Positions 22-135.
ModBaseSearch...

Protein-protein interaction databases

STRINGP10966.

Proteomic databases

PRIDEP10966.

Genome annotation databases

EnsemblENST00000390655; ENSP00000375070; ENSG00000172116; Homo sapiens. [Genome view]
GeneID926.
KEGGhsa:926.
UCSCuc002srx.1. human.
uc002sry.1. human.
uc002srz.1. human.

Organism-specific databases

CTD926.
GeneCardsGC02M086895.
H-InvDBHIX0024242.
HIX0030554.
HGNCHGNC:1707. CD8B.
HPACAB004353.
MIM186730. gene.
GenAtlasSearch...

Phylogenomic databases

HOVERGENP10966.
OMAVQTNQMV.

Enzyme and pathway databases

Pathway_Interaction_DBcd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
il12_2pathway. IL12-mediated signaling events.
cd8tcrpathway. TCR signaling in naive CD8+ T cells.
ReactomeREACT_6185. HIV Infection.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpressP10966.
BgeeP10966.
CleanExHS_CD8B.
GenevestigatorP10966.
GermOnlineENSG00000172116. Homo sapiens.

Family and domain databases

InterProIPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio3832.
SOURCESearch...

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Entry information

Entry nameCD8B_HUMAN
AccessionPrimary (citable) accession number: P10966
Secondary accession number(s): P14860 expand/collapse secondary AC list , P14861, Q15980, Q496E0, Q496E1, Q9UQ55
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 19, 2010
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents