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P10963 (PCKA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]

EC=4.1.1.49
Gene names
Name:PCK1
Synonyms:PEPC, PPC1
Ordered Locus Names:YKR097W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332032EBI-13770,EBI-701
RSP5P399402EBI-13770,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453
PRO_0000203875

Regions

Nucleotide binding250 – 2578ATP Potential

Experimental info

Sequence conflict95 – 10713PCSER…INRER → HVLKEHGLSTVK Ref.1
Sequence conflict1231A → R in CAA31488. Ref.1
Sequence conflict3221H → Q in CAA31488. Ref.1
Sequence conflict3571P → L in AAU09760. Ref.5
Sequence conflict4311K → N in AAA76693. Ref.2
Sequence conflict544 – 5496AGPQFE → DWSSIRVNETC in CAA31488. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P10963 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: C1A3669075052EC0

FASTA54960,983
        10         20         30         40         50         60 
MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL 

        70         80         90        100        110        120 
IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI 

       130        140        150        160        170        180 
VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL 

       190        200        210        220        230        240 
HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI 

       250        260        270        280        290        300 
QNGDVTLFFG LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP 

       310        320        330        340        350        360 
EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP CLADSHPKNI 

       370        380        390        400        410        420 
ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG VTEPEPTFSS CFGQPFLALH 

       430        440        450        460        470        480 
PIRYATMLAT KMSQHKANAY LINTGWTGSS YVSGGKRCPL KYTRAILDSI HDGSLANETY 

       490        500        510        520        530        540 
ETLPIFNLQV PTKVNGVPAE LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD 


VLAAGPQFE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from Saccharomyces cerevisiae."
Stucka R., Veldes-Hevia K.D., Gancedo C., Feldmann H.
Nucleic Acids Res. 16:10926-10926(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino acid sequence, site-directed mutagenesis, and microenvironment characteristics of cysteines 365 and 458."
Krautwurst H., Encinas M.V., Marcus F., Latshaw S.P., Kemp R.G., Frey P.A., Cardemil E.
Biochemistry 34:6382-6388(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"ATP-dependent Saccharomyces cerevisiae phospho enol pyruvate carboxykinase: isolation and sequence of a peptide containing a highly reactive cysteine."
Alvear M., Encinas M.V., Kemp R.G., Latshaw S.P., Cardemil E.
Biochim. Biophys. Acta 1119:35-38(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 359-376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13096 Genomic DNA. Translation: CAA31488.1.
U24234 mRNA. Translation: AAA76693.1.
Z28322 Genomic DNA. Translation: CAA82177.1.
AY723843 Genomic DNA. Translation: AAU09760.1.
BK006944 Genomic DNA. Translation: DAA09248.1.
PIRA56461.
RefSeqNP_013023.3. NM_001179887.3.

3D structure databases

ProteinModelPortalP10963.
SMRP10963. Positions 32-548.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34228. 78 interactions.
IntActP10963. 8 interactions.
MINTMINT-4083715.

Proteomic databases

MaxQBP10963.
PaxDbP10963.
PeptideAtlasP10963.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR097W; YKR097W; YKR097W.
GeneID853972.
KEGGsce:YKR097W.

Organism-specific databases

CYGDYKR097w.
SGDS000001805. PCK1.

Phylogenomic databases

eggNOGCOG1866.
HOGENOMHOG000271471.
KOK01610.
OMAHPGRYAK.
OrthoDBEOG7MKWFS.

Enzyme and pathway databases

BioCycYEAST:YKR097W-MONOMER.
SABIO-RKP10963.
UniPathwayUPA00138.

Gene expression databases

GenevestigatorP10963.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00453. PEPCK_ATP.
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975419.
PROP10963.

Entry information

Entry namePCKA_YEAST
AccessionPrimary (citable) accession number: P10963
Secondary accession number(s): D6VXF8, E9P963
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways