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Protein

Phosphoenolpyruvate carboxykinase (ATP)

Gene

PCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 257ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoenolpyruvate carboxykinase (ATP) activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: SGD

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YKR097W-MONOMER
BRENDAi4.1.1.32 984
4.1.1.49 984
SABIO-RKiP10963
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase (ATP) (EC:4.1.1.49)
Gene namesi
Name:PCK1
Synonyms:PEPC, PPC1
Ordered Locus Names:YKR097W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR097W
SGDiS000001805 PCK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002038751 – 549Phosphoenolpyruvate carboxykinase (ATP)Add BLAST549

Proteomic databases

MaxQBiP10963
PaxDbiP10963
PRIDEiP10963

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34228, 107 interactors
IntActiP10963, 8 interactors
MINTiP10963
STRINGi4932.YKR097W

Structurei

3D structure databases

ProteinModelPortaliP10963
SMRiP10963
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000271471
InParanoidiP10963
KOiK01610
OMAiMRYAGEM
OrthoDBiEOG092C1G01

Family and domain databases

CDDicd00484 PEPCK_ATP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00453 PEPCK_ATP, 1 hit
InterProiView protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS
PANTHERiPTHR30031 PTHR30031, 1 hit
PfamiView protein in Pfam
PF01293 PEPCK_ATP, 1 hit
PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
TIGRFAMsiTIGR00224 pckA, 1 hit
PROSITEiView protein in PROSITE
PS00532 PEPCK_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P10963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN
60 70 80 90 100
KTVISSSGAL IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT
110 120 130 140 150
WSINRERAAD YLRTRDHIYI VDAFAGWDPK YRIKVRVVCA RAYHALFMTN
160 170 180 190 200
MLIRPTEEEL AHFGEPDFTV WNAGQFPANL HTQDMSSKST IEINFKAMEM
210 220 230 240 250
IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI QNGDVTLFFG
260 270 280 290 300
LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP
310 320 330 340 350
EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP
360 370 380 390 400
CLADSHPKNI ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG
410 420 430 440 450
VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKANAY LINTGWTGSS
460 470 480 490 500
YVSGGKRCPL KYTRAILDSI HDGSLANETY ETLPIFNLQV PTKVNGVPAE
510 520 530 540
LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD VLAAGPQFE
Length:549
Mass (Da):60,983
Last modified:June 1, 1994 - v2
Checksum:iC1A3669075052EC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95 – 107PCSER…INRER → HVLKEHGLSTVK (PubMed:3060853).CuratedAdd BLAST13
Sequence conflicti123A → R in CAA31488 (PubMed:3060853).Curated1
Sequence conflicti322H → Q in CAA31488 (PubMed:3060853).Curated1
Sequence conflicti357P → L in AAU09760 (PubMed:17322287).Curated1
Sequence conflicti431K → N in AAA76693 (PubMed:7756267).Curated1
Sequence conflicti544 – 549AGPQFE → DWSSIRVNETC in CAA31488 (PubMed:3060853).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13096 Genomic DNA Translation: CAA31488.1
U24234 mRNA Translation: AAA76693.1
Z28322 Genomic DNA Translation: CAA82177.1
AY723843 Genomic DNA Translation: AAU09760.1
BK006944 Genomic DNA Translation: DAA09248.1
PIRiA56461
RefSeqiNP_013023.3, NM_001179887.3

Genome annotation databases

EnsemblFungiiYKR097W; YKR097W; YKR097W
GeneIDi853972
KEGGisce:YKR097W

Similar proteinsi

Entry informationi

Entry nameiPCKA_YEAST
AccessioniPrimary (citable) accession number: P10963
Secondary accession number(s): D6VXF8, E9P963
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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