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P10963

- PCKA_YEAST

UniProt

P10963 - PCKA_YEAST

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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene
PCK1, PEPC, PPC1, YKR097W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi250 – 2578ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoenolpyruvate carboxykinase (ATP) activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. gluconeogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YKR097W-MONOMER.
SABIO-RKP10963.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] (EC:4.1.1.49)
Gene namesi
Name:PCK1
Synonyms:PEPC, PPC1
Ordered Locus Names:YKR097W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR097w.
SGDiS000001805. PCK1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Phosphoenolpyruvate carboxykinase [ATP]UniRule annotationPRO_0000203875Add
BLAST

Proteomic databases

MaxQBiP10963.
PaxDbiP10963.
PeptideAtlasiP10963.

Expressioni

Gene expression databases

GenevestigatoriP10963.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332032EBI-13770,EBI-701
RSP5P399402EBI-13770,EBI-16219

Protein-protein interaction databases

BioGridi34228. 78 interactions.
IntActiP10963. 8 interactions.
MINTiMINT-4083715.

Structurei

3D structure databases

ProteinModelPortaliP10963.
SMRiP10963. Positions 32-548.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
KOiK01610.
OMAiHPGRYAK.
OrthoDBiEOG7MKWFS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10963-1 [UniParc]FASTAAdd to Basket

« Hide

MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN    50
KTVISSSGAL IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT 100
WSINRERAAD YLRTRDHIYI VDAFAGWDPK YRIKVRVVCA RAYHALFMTN 150
MLIRPTEEEL AHFGEPDFTV WNAGQFPANL HTQDMSSKST IEINFKAMEM 200
IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI QNGDVTLFFG 250
LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP 300
EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP 350
CLADSHPKNI ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG 400
VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKANAY LINTGWTGSS 450
YVSGGKRCPL KYTRAILDSI HDGSLANETY ETLPIFNLQV PTKVNGVPAE 500
LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD VLAAGPQFE 549
Length:549
Mass (Da):60,983
Last modified:June 1, 1994 - v2
Checksum:iC1A3669075052EC0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 10713PCSER…INRER → HVLKEHGLSTVK1 PublicationAdd
BLAST
Sequence conflicti123 – 1231A → R in CAA31488. 1 Publication
Sequence conflicti322 – 3221H → Q in CAA31488. 1 Publication
Sequence conflicti357 – 3571P → L in AAU09760. 1 Publication
Sequence conflicti431 – 4311K → N in AAA76693. 1 Publication
Sequence conflicti544 – 5496AGPQFE → DWSSIRVNETC in CAA31488. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13096 Genomic DNA. Translation: CAA31488.1.
U24234 mRNA. Translation: AAA76693.1.
Z28322 Genomic DNA. Translation: CAA82177.1.
AY723843 Genomic DNA. Translation: AAU09760.1.
BK006944 Genomic DNA. Translation: DAA09248.1.
PIRiA56461.
RefSeqiNP_013023.3. NM_001179887.3.

Genome annotation databases

EnsemblFungiiYKR097W; YKR097W; YKR097W.
GeneIDi853972.
KEGGisce:YKR097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13096 Genomic DNA. Translation: CAA31488.1 .
U24234 mRNA. Translation: AAA76693.1 .
Z28322 Genomic DNA. Translation: CAA82177.1 .
AY723843 Genomic DNA. Translation: AAU09760.1 .
BK006944 Genomic DNA. Translation: DAA09248.1 .
PIRi A56461.
RefSeqi NP_013023.3. NM_001179887.3.

3D structure databases

ProteinModelPortali P10963.
SMRi P10963. Positions 32-548.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34228. 78 interactions.
IntActi P10963. 8 interactions.
MINTi MINT-4083715.

Proteomic databases

MaxQBi P10963.
PaxDbi P10963.
PeptideAtlasi P10963.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR097W ; YKR097W ; YKR097W .
GeneIDi 853972.
KEGGi sce:YKR097W.

Organism-specific databases

CYGDi YKR097w.
SGDi S000001805. PCK1.

Phylogenomic databases

eggNOGi COG1866.
HOGENOMi HOG000271471.
KOi K01610.
OMAi HPGRYAK.
OrthoDBi EOG7MKWFS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci YEAST:YKR097W-MONOMER.
SABIO-RK P10963.

Miscellaneous databases

NextBioi 975419.
PROi P10963.

Gene expression databases

Genevestigatori P10963.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00453. PEPCK_ATP.
InterProi IPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view ]
Pfami PF01293. PEPCK_ATP. 1 hit.
[Graphical view ]
PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
TIGRFAMsi TIGR00224. pckA. 1 hit.
PROSITEi PS00532. PEPCK_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from Saccharomyces cerevisiae."
    Stucka R., Veldes-Hevia K.D., Gancedo C., Feldmann H.
    Nucleic Acids Res. 16:10926-10926(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino acid sequence, site-directed mutagenesis, and microenvironment characteristics of cysteines 365 and 458."
    Krautwurst H., Encinas M.V., Marcus F., Latshaw S.P., Kemp R.G., Frey P.A., Cardemil E.
    Biochemistry 34:6382-6388(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "ATP-dependent Saccharomyces cerevisiae phospho enol pyruvate carboxykinase: isolation and sequence of a peptide containing a highly reactive cysteine."
    Alvear M., Encinas M.V., Kemp R.G., Latshaw S.P., Cardemil E.
    Biochim. Biophys. Acta 1119:35-38(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 359-376.

Entry informationi

Entry nameiPCKA_YEAST
AccessioniPrimary (citable) accession number: P10963
Secondary accession number(s): D6VXF8, E9P963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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