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P10963

- PCKA_YEAST

UniProt

P10963 - PCKA_YEAST

Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

PCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi250 – 2578ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoenolpyruvate carboxykinase (ATP) activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. gluconeogenesis Source: SGD

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YKR097W-MONOMER.
    SABIO-RKP10963.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [ATP] (EC:4.1.1.49)
    Gene namesi
    Name:PCK1
    Synonyms:PEPC, PPC1
    Ordered Locus Names:YKR097W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR097w.
    SGDiS000001805. PCK1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Phosphoenolpyruvate carboxykinase [ATP]PRO_0000203875Add
    BLAST

    Proteomic databases

    MaxQBiP10963.
    PaxDbiP10963.
    PeptideAtlasiP10963.

    Expressioni

    Gene expression databases

    GenevestigatoriP10963.

    Interactioni

    Subunit structurei

    Homotetramer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP40P332032EBI-13770,EBI-701
    RSP5P399402EBI-13770,EBI-16219

    Protein-protein interaction databases

    BioGridi34228. 78 interactions.
    IntActiP10963. 8 interactions.
    MINTiMINT-4083715.

    Structurei

    3D structure databases

    ProteinModelPortaliP10963.
    SMRiP10963. Positions 32-548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1866.
    HOGENOMiHOG000271471.
    KOiK01610.
    OMAiHPGRYAK.
    OrthoDBiEOG7MKWFS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00453. PEPCK_ATP.
    InterProiIPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view]
    PfamiPF01293. PEPCK_ATP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    TIGRFAMsiTIGR00224. pckA. 1 hit.
    PROSITEiPS00532. PEPCK_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10963-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN    50
    KTVISSSGAL IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT 100
    WSINRERAAD YLRTRDHIYI VDAFAGWDPK YRIKVRVVCA RAYHALFMTN 150
    MLIRPTEEEL AHFGEPDFTV WNAGQFPANL HTQDMSSKST IEINFKAMEM 200
    IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI QNGDVTLFFG 250
    LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP 300
    EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP 350
    CLADSHPKNI ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG 400
    VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKANAY LINTGWTGSS 450
    YVSGGKRCPL KYTRAILDSI HDGSLANETY ETLPIFNLQV PTKVNGVPAE 500
    LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD VLAAGPQFE 549
    Length:549
    Mass (Da):60,983
    Last modified:June 1, 1994 - v2
    Checksum:iC1A3669075052EC0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 10713PCSER…INRER → HVLKEHGLSTVK(PubMed:3060853)CuratedAdd
    BLAST
    Sequence conflicti123 – 1231A → R in CAA31488. (PubMed:3060853)Curated
    Sequence conflicti322 – 3221H → Q in CAA31488. (PubMed:3060853)Curated
    Sequence conflicti357 – 3571P → L in AAU09760. (PubMed:17322287)Curated
    Sequence conflicti431 – 4311K → N in AAA76693. (PubMed:7756267)Curated
    Sequence conflicti544 – 5496AGPQFE → DWSSIRVNETC in CAA31488. (PubMed:3060853)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13096 Genomic DNA. Translation: CAA31488.1.
    U24234 mRNA. Translation: AAA76693.1.
    Z28322 Genomic DNA. Translation: CAA82177.1.
    AY723843 Genomic DNA. Translation: AAU09760.1.
    BK006944 Genomic DNA. Translation: DAA09248.1.
    PIRiA56461.
    RefSeqiNP_013023.3. NM_001179887.3.

    Genome annotation databases

    EnsemblFungiiYKR097W; YKR097W; YKR097W.
    GeneIDi853972.
    KEGGisce:YKR097W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13096 Genomic DNA. Translation: CAA31488.1 .
    U24234 mRNA. Translation: AAA76693.1 .
    Z28322 Genomic DNA. Translation: CAA82177.1 .
    AY723843 Genomic DNA. Translation: AAU09760.1 .
    BK006944 Genomic DNA. Translation: DAA09248.1 .
    PIRi A56461.
    RefSeqi NP_013023.3. NM_001179887.3.

    3D structure databases

    ProteinModelPortali P10963.
    SMRi P10963. Positions 32-548.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34228. 78 interactions.
    IntActi P10963. 8 interactions.
    MINTi MINT-4083715.

    Proteomic databases

    MaxQBi P10963.
    PaxDbi P10963.
    PeptideAtlasi P10963.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR097W ; YKR097W ; YKR097W .
    GeneIDi 853972.
    KEGGi sce:YKR097W.

    Organism-specific databases

    CYGDi YKR097w.
    SGDi S000001805. PCK1.

    Phylogenomic databases

    eggNOGi COG1866.
    HOGENOMi HOG000271471.
    KOi K01610.
    OMAi HPGRYAK.
    OrthoDBi EOG7MKWFS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci YEAST:YKR097W-MONOMER.
    SABIO-RK P10963.

    Miscellaneous databases

    NextBioi 975419.
    PROi P10963.

    Gene expression databases

    Genevestigatori P10963.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00453. PEPCK_ATP.
    InterProi IPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view ]
    Pfami PF01293. PEPCK_ATP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    TIGRFAMsi TIGR00224. pckA. 1 hit.
    PROSITEi PS00532. PEPCK_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from Saccharomyces cerevisiae."
      Stucka R., Veldes-Hevia K.D., Gancedo C., Feldmann H.
      Nucleic Acids Res. 16:10926-10926(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino acid sequence, site-directed mutagenesis, and microenvironment characteristics of cysteines 365 and 458."
      Krautwurst H., Encinas M.V., Marcus F., Latshaw S.P., Kemp R.G., Frey P.A., Cardemil E.
      Biochemistry 34:6382-6388(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "ATP-dependent Saccharomyces cerevisiae phospho enol pyruvate carboxykinase: isolation and sequence of a peptide containing a highly reactive cysteine."
      Alvear M., Encinas M.V., Kemp R.G., Latshaw S.P., Cardemil E.
      Biochim. Biophys. Acta 1119:35-38(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 359-376.

    Entry informationi

    Entry nameiPCKA_YEAST
    AccessioniPrimary (citable) accession number: P10963
    Secondary accession number(s): D6VXF8, E9P963
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3