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Reviewed, UniProtKB/Swiss-Prot P10963 (PCKA_YEAST)

Last modified June 16, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoenolpyruvate carboxykinase [ATP]
    EC=4.1.1.49
Gene names
Name: PCK1
Synonyms: PEPC, PPC1
Ordered Locus Names: YKR097W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Phosphoenolpyruvate carboxykinase [ATP]
PRO_0000203875

Regions

Nucleotide binding250 – 2578ATP Potential

Amino acid modifications

Modified residue3301Phosphoserine Ref.5

Experimental info

Sequence conflict95 – 10713PCSER…INRER → HVLKEHGLSTVK Ref.1
Sequence conflict1231A → R in CAA31488. Ref.1
Sequence conflict3221H → Q in CAA31488. Ref.1
Sequence conflict4311K → N in AAA76693. Ref.2
Sequence conflict544 – 5496AGPQFE → DWSSIRVNETC in CAA31488. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P10963-1 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: C1A3669075052EC0

FASTA54960,983
        10         20         30         40         50         60 
MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL 

        70         80         90        100        110        120 
IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI 

       130        140        150        160        170        180 
VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL 

       190        200        210        220        230        240 
HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI 

       250        260        270        280        290        300 
QNGDVTLFFG LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP 

       310        320        330        340        350        360 
EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP CLADSHPKNI 

       370        380        390        400        410        420 
ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG VTEPEPTFSS CFGQPFLALH 

       430        440        450        460        470        480 
PIRYATMLAT KMSQHKANAY LINTGWTGSS YVSGGKRCPL KYTRAILDSI HDGSLANETY 

       490        500        510        520        530        540 
ETLPIFNLQV PTKVNGVPAE LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD 


VLAAGPQFE

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from Saccharomyces cerevisiae."
Stucka R., Veldes-Hevia K.D., Gancedo C., Feldmann H.
Nucleic Acids Res. 16:10926-10926(1988) [PubMed: 3060853] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino acid sequence, site-directed mutagenesis, and microenvironment characteristics of cysteines 365 and 458."
Krautwurst H., Encinas M.V., Marcus F., Latshaw S.P., Kemp R.G., Frey P.A., Cardemil E.
Biochemistry 34:6382-6388(1995) [PubMed: 7756267] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"ATP-dependent Saccharomyces cerevisiae phospho enol pyruvate carboxykinase: isolation and sequence of a peptide containing a highly reactive cysteine."
Alvear M., Encinas M.V., Kemp R.G., Latshaw S.P., Cardemil E.
Biochim. Biophys. Acta 1119:35-38(1992) [PubMed: 1540632] [Abstract]
Cited for: PROTEIN SEQUENCE OF 359-376.
[5]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X13096 Genomic DNA. Translation: CAA31488.1.
U24234 mRNA. Translation: AAA76693.1.
Z28322 Genomic DNA. Translation: CAA82177.1.
PIRA56461.
RefSeqNP_013023.1.

3D structure databases

HSSPHSSP built from PDB template 1II2 based on UniProtKB P51058.
ModBaseSearch...

Protein-protein interaction databases

IntActP10963. 9 interactions.

Proteomic databases

PeptideAtlasP10963.

Genome annotation databases

EnsemblYKR097W. Saccharomyces cerevisiae. [Contig view]
GeneID853972.
GenomeReviewsGene locus YKR097W in contig Y13137_GR.
KEGGsce:YKR097W.
NMPDRfig|4932.3.peg.4011.

Organism-specific databases

CYGDYKR097w.
SGDS000001805. PCK1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP10963.
OMAP10963. MFIRPTE.

Enzyme and pathway databases

BRENDA4.1.1.49. 250.

Gene expression databases

ArrayExpressP10963.
GermOnlineYKR097W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR015590. Aldehyde_DH.
IPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
ProDomPD004738. PEPCK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio975419.

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Entry information

Entry namePCKA_YEAST
AccessionPrimary (citable) accession number: P10963
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents