P10962 (MAK16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MAK16

Alternative name(s):
Maintenance of killer protein 16

Gene names

Name:	MAK16
Ordered Locus Names:	YAL025C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Its role might be as part of the apparatus concerned with the nuclear events of the cell cycle. Ref.1
Subcellular location	Nucleus.
Sequence similarities	Belongs to the MAK16 family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 16710831. Source: SGD maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 16710831. Source: SGD
Cellular_component	nucleolus Inferred from direct assay PubMed 10838225. Source: SGD preribosome, large subunit precursor Inferred from direct assay PubMed 11583614. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Protein MAK16		PRO_0000203793
Regions
	Repeat	213 – 220	8	1
	Repeat	222 – 229	8	2
	Region	213 – 229	17	2 X 8 AA repeats
	Region	251 – 264	14	7 X 2 AA repeats of S-[DE]
	Motif	139 – 144	6	Nuclear localization signal Potential
	Motif	282 – 287	6	Nuclear localization signal Potential
	Compositional bias	195 – 235	41	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	250	1	E → Q in AAA34752. Ref.1
	Sequence conflict	250	1	E → Q in AAC05007. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10962 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 309ED6897EA24F73	FASTA	306	35,696
	10 20 30 40 50 60 MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL 70 80 90 100 110 120 YLYMKTPERA HTPAKLWERI KLSKNYTKAL QQIDEHLLHW SKFFRHKCKQ RFTKLTQVMI 130 140 150 160 170 180 TERRLALREE ERHYVGVAPK VKRREQNRER KALVAAKIEK AIEKELMDRL KSGAYGDKPL 190 200 210 220 230 240 NVDEKVWKKI MGQMEEENSQ DEEEDWDEEE ESDDGEVEYV ADDGEGEYVD VDDLEKWLAD 250 260 270 280 290 300 SDREASSASE SESDSESESD SDSDEENKNS AKRRKKGTSA KTKRPKVEIE YEEEHEVQNA EQEVAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces cerevisiae." Wickner R.B. Proc. Natl. Acad. Sci. U.S.A. 85:6007-6011(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 250. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03852 Genomic DNA. Translation: AAA34752.1. U12980 Genomic DNA. Translation: AAC05007.1. BK006935 Genomic DNA. Translation: DAA06963.2.
PIR	BVBYK6. A35588.
RefSeq	NP_009377.2. NM_001178170.2.
3D structure databases
ProteinModelPortal	P10962.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6521N.
IntAct	P10962. 3 interactions.
MINT	MINT-485081.
STRING	4932.YAL025C.
Proteomic databases
PaxDb	P10962.
PeptideAtlas	P10962.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL025C; YAL025C; YAL025C.
GeneID	851208.
KEGG	sce:YAL025C.
Organism-specific databases
CYGD	YAL025c.
SGD	S000000023. MAK16.
Phylogenomic databases
eggNOG	COG5129.
GeneTree	ENSGT00390000012859.
HOGENOM	HOG000201538.
KO	K14831.
OMA	TQSFCRN.
OrthoDB	EOG45B4QX.
Gene expression databases
Genevestigator	P10962.
GermOnline	YAL025C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006958. Mak16. [Graphical view]
PANTHER	PTHR23405:SF4. PTHR23405:SF4. 1 hit.
Pfam	PF04874. Mak16. 1 hit. [Graphical view]
PIRSF	PIRSF003352. MAK16. 1 hit.
ProtoNet	Search...
Other
NextBio	968079.

Entry information

Entry name

MAK16_YEAST

Accession

Primary (citable) accession number: P10962
Secondary accession number(s): D6VPJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program