Sulfated glycoprotein 1 precursor - Rattus norvegicus (Rat)

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P10960 (SAP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sulfated glycoprotein 1
Short name=SGP-1

Alternative name(s):
Prosaposin

Gene names

Name:	Psap
Synonyms:	Sgp1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location

Secreted › extracellular space.

Sequence similarities

Contains 2 saposin A-type domains.

Contains 4 saposin B-type domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycolipid transport Non-traceable author statement Ref.2. Source: RGD sphingolipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

Chain

17 – 554

538

Sulfated glycoprotein 1

PRO_0000031629

Regions

Domain

18 – 58

Saposin A-type 1

Domain

59 – 142

Saposin B-type 1

Domain

193 – 274

Saposin B-type 2

Domain

310 – 391

Saposin B-type 3

Domain

435 – 516

Saposin B-type 4

Domain

518 – 554

Saposin A-type 2

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

214

N-linked (GlcNAc...) Potential

Glycosylation

331

N-linked (GlcNAc...) Potential

Glycosylation

456

N-linked (GlcNAc...) Potential

Disulfide bond

63 ↔ 138

By similarity

Disulfide bond

66 ↔ 132

By similarity

Disulfide bond

94 ↔ 106

By similarity

Disulfide bond

197 ↔ 270

By similarity

Disulfide bond

200 ↔ 264

By similarity

Disulfide bond

229 ↔ 240

By similarity

Disulfide bond

314 ↔ 387

By similarity

Disulfide bond

317 ↔ 381

By similarity

Disulfide bond

345 ↔ 356

By similarity

Disulfide bond

439 ↔ 512

By similarity

Disulfide bond

442 ↔ 506

By similarity

Disulfide bond

470 ↔ 481

By similarity

Experimental info

Sequence conflict

115

P → L Ref.2

Sequence conflict

115

P → L Ref.3

Sequence conflict

299

D → E in AAB36042. Ref.2

Sequence conflict

462

I → V in AAB36233. Ref.3

Sequence conflict

527

W → R in AAB36233. Ref.3

Sequence conflict

536

S → M in AAB36233. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P10960 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: DFE3F3A3A0520C6B
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FASTA

554

61,124

        10         20         30         40         50         60 
MYALALLASL LVTALTSPVQ DPKICSGGSA VVCRDVKTAV DCRAVKHCQQ MVWSKPTAKS 

        70         80         90        100        110        120 
LPCDICKTVV TEAGNLLKDN ATEEEILHYL EKTCAWIHDS SLSASCKEVV DSYLPVILDM 

       130        140        150        160        170        180 
IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ RQLESNKIPE VDLARVVAPF MSNIPLLLYP 

       190        200        210        220        230        240 
QDRPRSQPQP KANEDVCQDC MKLVTDIQTA VRTNSSFVQG LVDHVKEDCD RLGPGVSDIC 

       250        260        270        280        290        300 
KNYVDQYSEV AVQMMMHMQP KEICVMVGFC DEVKRVPMRT LVPATEAIKN ILPALELTDP 

       310        320        330        340        350        360 
YEQDVIQAQN VIFCQVCQLV MRKLSELIIN NATEELLIKG LSKACSLLPA PASTKCQEVL 

       370        380        390        400        410        420 
VTFGPSLLDV LMHEVNPNFL CGVISLCSAN PNLVGTLEQP AAAIVSALPK EPAPPKQPEE 

       430        440        450        460        470        480 
PKQSALRAHV PPQKNGGFCE VCKKLVIYLE HNLEKNSTKE EILAALEKGC SFLPDPYQKQ 

       490        500        510        520        530        540 
CDEFVAEYEP LLLEILVEVM DPSFVCSKIG VCPSAYKLLL GTEKCVWGPG YWCQNSETAA 

       550 
RCNAVDHCKR HVWN

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References

[1]	"Biosynthesis and molecular cloning of sulfated glycoprotein 1 secreted by rat Sertoli cells: sequence similarity with the 70-kilodalton precursor to sulfatide/GM1 activator." Collard M.W., Sylvester S.R., Tsuruta J.K., Griswold M.D. Biochemistry 27:4557-4564(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Sertoli cell.
[2]	"Molecular role of sulfated glycoprotein-1 (SGP-1/prosaposin) in Sertoli cells." Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A. Histol. Histopathol. 10:1023-1034(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"Expression and tissue distribution of rat sulfated glycoprotein-1 (prosaposin)." Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A. J. Histochem. Cytochem. 44:327-337(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[4]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 68-78; 108-122; 192-212; 323-343; 444-445 AND 460-479, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19936 mRNA. Translation: AAA42136.1. S81353 mRNA. Translation: AAB36042.2. S81373 mRNA. Translation: AAB36233.2.
IPI	IPI00195160.
PIR	A28716.
RefSeq	NP_001177165.1. NM_001190236.1. NP_037145.2. NM_013013.2.
UniGene	Rn.97173.
3D structure databases
ProteinModelPortal	P10960.
SMR	P10960. Positions 60-140, 195-271, 435-514.
ModBase	Search...
PTM databases
PhosphoSite	P10960.
Proteomic databases
PaxDb	P10960.
PRIDE	P10960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25524.
KEGG	rno:25524.
UCSC	RGD:3423. rat.
Organism-specific databases
CTD	5660.
RGD	3423. Psap.
Phylogenomic databases
eggNOG	NOG244726.
HOGENOM	HOG000049216.
HOVERGEN	HBG002617.
InParanoid	P10960.
KO	K12382.
OrthoDB	EOG4K3KW5.
Gene expression databases
ArrayExpress	P10960.
Genevestigator	P10960.
GermOnline	ENSRNOG00000000571. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.225.10. 4 hits.
InterPro	IPR003119. SapA. IPR007856. SapB_1. IPR008138. SapB_2. IPR008373. Saposin. IPR011001. Saposin-like. IPR021165. Saposin_chordata. IPR008139. SaposinB. [Graphical view]
Pfam	PF02199. SapA. 2 hits. PF05184. SapB_1. 4 hits. PF03489. SapB_2. 4 hits. [Graphical view]
PIRSF	PIRSF002431. Saposin. 1 hit.
PRINTS	PR01797. SAPOSIN.
SMART	SM00162. SAPA. 2 hits. SM00741. SapB. 4 hits. [Graphical view]
SUPFAM	SSF47862. Saposin_like. 4 hits.
PROSITE	PS51110. SAP_A. 2 hits. PS50015. SAP_B. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	606999.

Entry information

Entry name

SAP_RAT

Accession

Primary (citable) accession number: P10960
Secondary accession number(s): Q62841, Q64190

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents