Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10960 (SAP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sulfated glycoprotein 1

Short name=SGP-1
Alternative name(s):
Prosaposin
Gene names
Name:Psap
Synonyms:Sgp1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secretedextracellular space.

Sequence similarities

Contains 2 saposin A-type domains.

Contains 4 saposin B-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616
Chain17 – 554538Sulfated glycoprotein 1
PRO_0000031629

Regions

Domain18 – 5841Saposin A-type 1
Domain59 – 14284Saposin B-type 1
Domain193 – 27482Saposin B-type 2
Domain310 – 39182Saposin B-type 3
Domain435 – 51682Saposin B-type 4
Domain518 – 55437Saposin A-type 2

Amino acid modifications

Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation2141N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Disulfide bond63 ↔ 138 By similarity
Disulfide bond66 ↔ 132 By similarity
Disulfide bond94 ↔ 106 By similarity
Disulfide bond197 ↔ 270 By similarity
Disulfide bond200 ↔ 264 By similarity
Disulfide bond229 ↔ 240 By similarity
Disulfide bond314 ↔ 387 By similarity
Disulfide bond317 ↔ 381 By similarity
Disulfide bond345 ↔ 356 By similarity
Disulfide bond439 ↔ 512 By similarity
Disulfide bond442 ↔ 506 By similarity
Disulfide bond470 ↔ 481 By similarity

Experimental info

Sequence conflict1151P → L Ref.2
Sequence conflict1151P → L Ref.3
Sequence conflict2991D → E in AAB36042. Ref.2
Sequence conflict4621I → V in AAB36233. Ref.3
Sequence conflict5271W → R in AAB36233. Ref.3
Sequence conflict5361S → M in AAB36233. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P10960 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: DFE3F3A3A0520C6B

FASTA55461,124
        10         20         30         40         50         60 
MYALALLASL LVTALTSPVQ DPKICSGGSA VVCRDVKTAV DCRAVKHCQQ MVWSKPTAKS 

        70         80         90        100        110        120 
LPCDICKTVV TEAGNLLKDN ATEEEILHYL EKTCAWIHDS SLSASCKEVV DSYLPVILDM 

       130        140        150        160        170        180 
IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ RQLESNKIPE VDLARVVAPF MSNIPLLLYP 

       190        200        210        220        230        240 
QDRPRSQPQP KANEDVCQDC MKLVTDIQTA VRTNSSFVQG LVDHVKEDCD RLGPGVSDIC 

       250        260        270        280        290        300 
KNYVDQYSEV AVQMMMHMQP KEICVMVGFC DEVKRVPMRT LVPATEAIKN ILPALELTDP 

       310        320        330        340        350        360 
YEQDVIQAQN VIFCQVCQLV MRKLSELIIN NATEELLIKG LSKACSLLPA PASTKCQEVL 

       370        380        390        400        410        420 
VTFGPSLLDV LMHEVNPNFL CGVISLCSAN PNLVGTLEQP AAAIVSALPK EPAPPKQPEE 

       430        440        450        460        470        480 
PKQSALRAHV PPQKNGGFCE VCKKLVIYLE HNLEKNSTKE EILAALEKGC SFLPDPYQKQ 

       490        500        510        520        530        540 
CDEFVAEYEP LLLEILVEVM DPSFVCSKIG VCPSAYKLLL GTEKCVWGPG YWCQNSETAA 

       550 
RCNAVDHCKR HVWN 

« Hide

References

[1]"Biosynthesis and molecular cloning of sulfated glycoprotein 1 secreted by rat Sertoli cells: sequence similarity with the 70-kilodalton precursor to sulfatide/GM1 activator."
Collard M.W., Sylvester S.R., Tsuruta J.K., Griswold M.D.
Biochemistry 27:4557-4564(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Sertoli cell.
[2]"Molecular role of sulfated glycoprotein-1 (SGP-1/prosaposin) in Sertoli cells."
Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A.
Histol. Histopathol. 10:1023-1034(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]"Expression and tissue distribution of rat sulfated glycoprotein-1 (prosaposin)."
Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A.
J. Histochem. Cytochem. 44:327-337(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[4]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 68-78; 108-122; 192-212; 323-343; 444-445 AND 460-479, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19936 mRNA. Translation: AAA42136.1.
S81353 mRNA. Translation: AAB36042.2.
S81373 mRNA. Translation: AAB36233.2.
PIRA28716.
RefSeqNP_001177165.1. NM_001190236.1.
NP_037145.2. NM_013013.2.
UniGeneRn.97173.

3D structure databases

ProteinModelPortalP10960.
SMRP10960. Positions 60-140, 195-271, 435-514.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP10960.

Proteomic databases

PaxDbP10960.
PRIDEP10960.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25524.
KEGGrno:25524.
UCSCRGD:3423. rat.

Organism-specific databases

CTD5660.
RGD3423. Psap.

Phylogenomic databases

eggNOGNOG244726.
HOGENOMHOG000049216.
HOVERGENHBG002617.
InParanoidP10960.
KOK12382.
PhylomeDBP10960.

Gene expression databases

GenevestigatorP10960.

Family and domain databases

Gene3D1.10.225.10. 4 hits.
InterProIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR021165. Saposin_chordata.
IPR008139. SaposinB.
[Graphical view]
PfamPF02199. SapA. 2 hits.
PF05184. SapB_1. 4 hits.
PF03489. SapB_2. 4 hits.
[Graphical view]
PIRSFPIRSF002431. Saposin. 1 hit.
PRINTSPR01797. SAPOSIN.
SMARTSM00162. SAPA. 2 hits.
SM00741. SapB. 4 hits.
[Graphical view]
SUPFAMSSF47862. SSF47862. 3 hits.
PROSITEPS51110. SAP_A. 2 hits.
PS50015. SAP_B. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio606999.

Entry information

Entry nameSAP_RAT
AccessionPrimary (citable) accession number: P10960
Secondary accession number(s): Q62841, Q64190
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families