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P10960

- SAP_RAT

UniProt

P10960 - SAP_RAT

Protein

Sulfated glycoprotein 1

Gene

Psap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    GO - Biological processi

    1. glycolipid transport Source: RGD
    2. sphingolipid metabolic process Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfated glycoprotein 1
    Short name:
    SGP-1
    Alternative name(s):
    Prosaposin
    Gene namesi
    Name:Psap
    Synonyms:Sgp1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3423. Psap.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    2. lysosome Source: InterPro

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Add
    BLAST
    Chaini17 – 554538Sulfated glycoprotein 1PRO_0000031629Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi63 ↔ 138PROSITE-ProRule annotation
    Disulfide bondi66 ↔ 132PROSITE-ProRule annotation
    Glycosylationi80 – 801N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi94 ↔ 106PROSITE-ProRule annotation
    Disulfide bondi197 ↔ 270PROSITE-ProRule annotation
    Disulfide bondi200 ↔ 264PROSITE-ProRule annotation
    Glycosylationi214 – 2141N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi229 ↔ 240PROSITE-ProRule annotation
    Disulfide bondi314 ↔ 387PROSITE-ProRule annotation
    Disulfide bondi317 ↔ 381PROSITE-ProRule annotation
    Glycosylationi331 – 3311N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi345 ↔ 356PROSITE-ProRule annotation
    Disulfide bondi439 ↔ 512PROSITE-ProRule annotation
    Disulfide bondi442 ↔ 506PROSITE-ProRule annotation
    Glycosylationi456 – 4561N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi470 ↔ 481PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP10960.
    PRIDEiP10960.

    PTM databases

    PhosphoSiteiP10960.

    Expressioni

    Gene expression databases

    GenevestigatoriP10960.

    Structurei

    3D structure databases

    ProteinModelPortaliP10960.
    SMRiP10960. Positions 60-140, 195-271, 435-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 5841Saposin A-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini59 – 14284Saposin B-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 27482Saposin B-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini310 – 39182Saposin B-type 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 51682Saposin B-type 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini518 – 55437Saposin A-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 saposin A-type domains.PROSITE-ProRule annotation
    Contains 4 saposin B-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG244726.
    HOGENOMiHOG000049216.
    HOVERGENiHBG002617.
    InParanoidiP10960.
    KOiK12382.
    PhylomeDBiP10960.

    Family and domain databases

    Gene3Di1.10.225.10. 4 hits.
    InterProiIPR003119. SapA.
    IPR007856. SapB_1.
    IPR008138. SapB_2.
    IPR008373. Saposin.
    IPR011001. Saposin-like.
    IPR021165. Saposin_chordata.
    IPR008139. SaposinB.
    [Graphical view]
    PfamiPF02199. SapA. 2 hits.
    PF05184. SapB_1. 4 hits.
    PF03489. SapB_2. 4 hits.
    [Graphical view]
    PIRSFiPIRSF002431. Saposin. 1 hit.
    PRINTSiPR01797. SAPOSIN.
    SMARTiSM00162. SAPA. 2 hits.
    SM00741. SapB. 4 hits.
    [Graphical view]
    SUPFAMiSSF47862. SSF47862. 3 hits.
    PROSITEiPS51110. SAP_A. 2 hits.
    PS50015. SAP_B. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10960-1 [UniParc]FASTAAdd to Basket

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    MYALALLASL LVTALTSPVQ DPKICSGGSA VVCRDVKTAV DCRAVKHCQQ    50
    MVWSKPTAKS LPCDICKTVV TEAGNLLKDN ATEEEILHYL EKTCAWIHDS 100
    SLSASCKEVV DSYLPVILDM IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ 150
    RQLESNKIPE VDLARVVAPF MSNIPLLLYP QDRPRSQPQP KANEDVCQDC 200
    MKLVTDIQTA VRTNSSFVQG LVDHVKEDCD RLGPGVSDIC KNYVDQYSEV 250
    AVQMMMHMQP KEICVMVGFC DEVKRVPMRT LVPATEAIKN ILPALELTDP 300
    YEQDVIQAQN VIFCQVCQLV MRKLSELIIN NATEELLIKG LSKACSLLPA 350
    PASTKCQEVL VTFGPSLLDV LMHEVNPNFL CGVISLCSAN PNLVGTLEQP 400
    AAAIVSALPK EPAPPKQPEE PKQSALRAHV PPQKNGGFCE VCKKLVIYLE 450
    HNLEKNSTKE EILAALEKGC SFLPDPYQKQ CDEFVAEYEP LLLEILVEVM 500
    DPSFVCSKIG VCPSAYKLLL GTEKCVWGPG YWCQNSETAA RCNAVDHCKR 550
    HVWN 554
    Length:554
    Mass (Da):61,124
    Last modified:July 1, 1989 - v1
    Checksum:iDFE3F3A3A0520C6B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151P → L(PubMed:8573994)Curated
    Sequence conflicti115 – 1151P → L(PubMed:8601692)Curated
    Sequence conflicti299 – 2991D → E in AAB36042. (PubMed:8573994)Curated
    Sequence conflicti462 – 4621I → V in AAB36233. (PubMed:8601692)Curated
    Sequence conflicti527 – 5271W → R in AAB36233. (PubMed:8601692)Curated
    Sequence conflicti536 – 5361S → M in AAB36233. (PubMed:8601692)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19936 mRNA. Translation: AAA42136.1.
    S81353 mRNA. Translation: AAB36042.2.
    S81373 mRNA. Translation: AAB36233.2.
    PIRiA28716.
    RefSeqiNP_001177165.1. NM_001190236.1.
    NP_037145.2. NM_013013.2.
    UniGeneiRn.97173.

    Genome annotation databases

    GeneIDi25524.
    KEGGirno:25524.
    UCSCiRGD:3423. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19936 mRNA. Translation: AAA42136.1 .
    S81353 mRNA. Translation: AAB36042.2 .
    S81373 mRNA. Translation: AAB36233.2 .
    PIRi A28716.
    RefSeqi NP_001177165.1. NM_001190236.1.
    NP_037145.2. NM_013013.2.
    UniGenei Rn.97173.

    3D structure databases

    ProteinModelPortali P10960.
    SMRi P10960. Positions 60-140, 195-271, 435-514.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P10960.

    Proteomic databases

    PaxDbi P10960.
    PRIDEi P10960.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25524.
    KEGGi rno:25524.
    UCSCi RGD:3423. rat.

    Organism-specific databases

    CTDi 5660.
    RGDi 3423. Psap.

    Phylogenomic databases

    eggNOGi NOG244726.
    HOGENOMi HOG000049216.
    HOVERGENi HBG002617.
    InParanoidi P10960.
    KOi K12382.
    PhylomeDBi P10960.

    Miscellaneous databases

    NextBioi 606999.

    Gene expression databases

    Genevestigatori P10960.

    Family and domain databases

    Gene3Di 1.10.225.10. 4 hits.
    InterProi IPR003119. SapA.
    IPR007856. SapB_1.
    IPR008138. SapB_2.
    IPR008373. Saposin.
    IPR011001. Saposin-like.
    IPR021165. Saposin_chordata.
    IPR008139. SaposinB.
    [Graphical view ]
    Pfami PF02199. SapA. 2 hits.
    PF05184. SapB_1. 4 hits.
    PF03489. SapB_2. 4 hits.
    [Graphical view ]
    PIRSFi PIRSF002431. Saposin. 1 hit.
    PRINTSi PR01797. SAPOSIN.
    SMARTi SM00162. SAPA. 2 hits.
    SM00741. SapB. 4 hits.
    [Graphical view ]
    SUPFAMi SSF47862. SSF47862. 3 hits.
    PROSITEi PS51110. SAP_A. 2 hits.
    PS50015. SAP_B. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis and molecular cloning of sulfated glycoprotein 1 secreted by rat Sertoli cells: sequence similarity with the 70-kilodalton precursor to sulfatide/GM1 activator."
      Collard M.W., Sylvester S.R., Tsuruta J.K., Griswold M.D.
      Biochemistry 27:4557-4564(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Sertoli cell.
    2. "Molecular role of sulfated glycoprotein-1 (SGP-1/prosaposin) in Sertoli cells."
      Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A.
      Histol. Histopathol. 10:1023-1034(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    3. "Expression and tissue distribution of rat sulfated glycoprotein-1 (prosaposin)."
      Morales C.R., El-Alfy M., Zhao Q., Igdoura S.A.
      J. Histochem. Cytochem. 44:327-337(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    4. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 68-78; 108-122; 192-212; 323-343; 444-445 AND 460-479, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiSAP_RAT
    AccessioniPrimary (citable) accession number: P10960
    Secondary accession number(s): Q62841, Q64190
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3