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Protein

Transcriptional regulatory protein FixJ

Gene

fixJ

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Magnesium1
Metal bindingi11Magnesium1
Metal bindingi54Magnesium1
Metal bindingi56Magnesium; via carbonyl oxygen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi159 – 178H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein FixJ
Gene namesi
Name:fixJ
Ordered Locus Names:RA0669
ORF Names:SMa1227
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811021 – 204Transcriptional regulatory protein FixJAdd BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by FixL.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP10958.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000900.

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 12Combined sources8
Helixi13 – 25Combined sources13
Beta strandi29 – 34Combined sources6
Helixi36 – 42Combined sources7
Helixi43 – 45Combined sources3
Beta strandi48 – 54Combined sources7
Beta strandi58 – 60Combined sources3
Helixi62 – 71Combined sources10
Beta strandi78 – 82Combined sources5
Helixi87 – 95Combined sources9
Beta strandi99 – 105Combined sources7
Helixi108 – 119Combined sources12
Helixi133 – 142Combined sources10
Helixi144 – 153Combined sources10
Turni154 – 156Combined sources3
Helixi159 – 165Combined sources7
Helixi170 – 183Combined sources14
Helixi189 – 199Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5WX-ray2.30A/B/C1-124[»]
1DBWX-ray1.60A/B1-124[»]
1DCKX-ray2.00A/B1-124[»]
1DCMX-ray3.00A/B1-124[»]
1X3UNMR-A130-204[»]
ProteinModelPortaliP10958.
SMRiP10958.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10958.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 119Response regulatoryPROSITE-ProRule annotationAdd BLAST115
Domaini135 – 200HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000034813.
KOiK14987.
OMAiHRANIFH.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYTVHIVD DEEPVRKSLA FMLTMNGFAV KMHQSAEAFL AFAPDVRNGV
60 70 80 90 100
LVTDLRMPDM SGVELLRNLG DLKINIPSIV ITGHGDVPMA VEAMKAGAVD
110 120 130 140 150
FIEKPFEDTV IIEAIERASE HLVAAEADVD DANDIRARLQ TLSERERQVL
160 170 180 190 200
SAVVAGLPNK SIAYDLDISP RTVEVHRANV MAKMKAKSLP HLVRMALAGG

FGPS
Length:204
Mass (Da):22,219
Last modified:July 1, 1989 - v1
Checksum:i2EDA356967352292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21854 Genomic DNA. Translation: CAA79898.1.
AE006469 Genomic DNA. Translation: AAK65327.1.
X15079 Genomic DNA. Translation: CAA33182.1.
PIRiB31227.
E95345.
RefSeqiNP_435915.1. NC_003037.1.
WP_010967648.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65327; AAK65327; SMa1227.
GeneIDi1235705.
25011576.
KEGGisme:SMa1227.
PATRICi23627820. VBISinMel96828_0689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21854 Genomic DNA. Translation: CAA79898.1.
AE006469 Genomic DNA. Translation: AAK65327.1.
X15079 Genomic DNA. Translation: CAA33182.1.
PIRiB31227.
E95345.
RefSeqiNP_435915.1. NC_003037.1.
WP_010967648.1. NC_003037.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5WX-ray2.30A/B/C1-124[»]
1DBWX-ray1.60A/B1-124[»]
1DCKX-ray2.00A/B1-124[»]
1DCMX-ray3.00A/B1-124[»]
1X3UNMR-A130-204[»]
ProteinModelPortaliP10958.
SMRiP10958.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP10958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK65327; AAK65327; SMa1227.
GeneIDi1235705.
25011576.
KEGGisme:SMa1227.
PATRICi23627820. VBISinMel96828_0689.

Phylogenomic databases

HOGENOMiHOG000034813.
KOiK14987.
OMAiHRANIFH.

Miscellaneous databases

EvolutionaryTraceiP10958.

Gene expression databases

CollecTFiEXPREG_00000900.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIXJ_RHIME
AccessioniPrimary (citable) accession number: P10958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.