Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcriptional regulatory protein FixJ

Gene

fixJ

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium
Metal bindingi11 – 111Magnesium
Metal bindingi54 – 541Magnesium
Metal bindingi56 – 561Magnesium; via carbonyl oxygen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi159 – 17820H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-5677-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein FixJ
Gene namesi
Name:fixJ
Ordered Locus Names:RA0669
ORF Names:SMa1227
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Transcriptional regulatory protein FixJPRO_0000081102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 5414-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by FixL.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

CollecTFiEXPREG_00000900.

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 128Combined sources
Helixi13 – 2513Combined sources
Beta strandi29 – 346Combined sources
Helixi36 – 427Combined sources
Helixi43 – 453Combined sources
Beta strandi48 – 547Combined sources
Beta strandi58 – 603Combined sources
Helixi62 – 7110Combined sources
Beta strandi78 – 825Combined sources
Helixi87 – 959Combined sources
Beta strandi99 – 1057Combined sources
Helixi108 – 11912Combined sources
Helixi133 – 14210Combined sources
Helixi144 – 15310Combined sources
Turni154 – 1563Combined sources
Helixi159 – 1657Combined sources
Helixi170 – 18314Combined sources
Helixi189 – 19911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5WX-ray2.30A/B/C1-124[»]
1DBWX-ray1.60A/B1-124[»]
1DCKX-ray2.00A/B1-124[»]
1DCMX-ray3.00A/B1-124[»]
1X3UNMR-A130-204[»]
ProteinModelPortaliP10958.
SMRiP10958. Positions 3-123, 129-204.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10958.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 119115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini135 – 20066HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000034813.
KOiK14987.
OMAiQKPFKED.
OrthoDBiEOG6QRWCT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYTVHIVD DEEPVRKSLA FMLTMNGFAV KMHQSAEAFL AFAPDVRNGV
60 70 80 90 100
LVTDLRMPDM SGVELLRNLG DLKINIPSIV ITGHGDVPMA VEAMKAGAVD
110 120 130 140 150
FIEKPFEDTV IIEAIERASE HLVAAEADVD DANDIRARLQ TLSERERQVL
160 170 180 190 200
SAVVAGLPNK SIAYDLDISP RTVEVHRANV MAKMKAKSLP HLVRMALAGG

FGPS
Length:204
Mass (Da):22,219
Last modified:July 1, 1989 - v1
Checksum:i2EDA356967352292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21854 Genomic DNA. Translation: CAA79898.1.
AE006469 Genomic DNA. Translation: AAK65327.1.
X15079 Genomic DNA. Translation: CAA33182.1.
PIRiB31227.
E95345.
RefSeqiNP_435915.1. NC_003037.1.
WP_010967648.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65327; AAK65327; SMa1227.
GeneIDi1235705.
25011576.
KEGGisme:SMa1227.
PATRICi23627820. VBISinMel96828_0689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21854 Genomic DNA. Translation: CAA79898.1.
AE006469 Genomic DNA. Translation: AAK65327.1.
X15079 Genomic DNA. Translation: CAA33182.1.
PIRiB31227.
E95345.
RefSeqiNP_435915.1. NC_003037.1.
WP_010967648.1. NC_003037.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5WX-ray2.30A/B/C1-124[»]
1DBWX-ray1.60A/B1-124[»]
1DCKX-ray2.00A/B1-124[»]
1DCMX-ray3.00A/B1-124[»]
1X3UNMR-A130-204[»]
ProteinModelPortaliP10958.
SMRiP10958. Positions 3-123, 129-204.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK65327; AAK65327; SMa1227.
GeneIDi1235705.
25011576.
KEGGisme:SMa1227.
PATRICi23627820. VBISinMel96828_0689.

Phylogenomic databases

HOGENOMiHOG000034813.
KOiK14987.
OMAiQKPFKED.
OrthoDBiEOG6QRWCT.

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-5677-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP10958.

Gene expression databases

CollecTFiEXPREG_00000900.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cascade regulation of nif gene expression in Rhizobium meliloti."
    David M., Daveran M.-L., Batut J., Dedieu A., Domergue O., Ghai J., Hertig C., Boistard P., Kahn D.
    Cell 54:671-683(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  4. "fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti."
    Batut J., Daveran-Mingot M.-L., David M., Jacobs J., Garnerone A.-M., Kahn D.
    EMBO J. 8:1279-1286(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 200-204.
  5. "Modular structure of FixJ: homology of the transcriptional activator domain with the -35 binding domain of sigma factors."
    Kahn D., Ditta G.S.
    Mol. Microbiol. 5:987-997(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, MUTAGENESIS OF HTH REGION.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-126.

Entry informationi

Entry nameiFIXJ_RHIME
AccessioniPrimary (citable) accession number: P10958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 20, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.