Skip Header

Contribute Send feedback
Read comments (?) or add your own

P10958 (FIXJ_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulatory protein fixJ
Gene names
Name:fixJ
Ordered Locus Names:RA0669
ORF Names:SMa1227
Encoded onPlasmid pSymA (megaplasmid 1)
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation.

Cofactor

Binds 1 magnesium ion per subunit.

Subcellular location

Cytoplasm Probable.

Post-translational modification

Phosphorylated by fixL.

Sequence similarities

Contains 1 HTH luxR-type DNA-binding domain.

Contains 1 response regulatory domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Transcriptional regulatory protein fixJ
PRO_0000081102

Regions

Domain1 – 119119Response regulatory
Domain135 – 20066HTH luxR-type
DNA binding159 – 17820H-T-H motif By similarity

Sites

Metal binding101Magnesium
Metal binding111Magnesium
Metal binding541Magnesium
Metal binding561Magnesium; via carbonyl oxygen

Amino acid modifications

Modified residue5414-aspartylphosphate

Secondary structure

1 .................................... 204
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10958 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 2EDA356967352292

FASTA20422,219
        10         20         30         40         50         60 
MTDYTVHIVD DEEPVRKSLA FMLTMNGFAV KMHQSAEAFL AFAPDVRNGV LVTDLRMPDM 

        70         80         90        100        110        120 
SGVELLRNLG DLKINIPSIV ITGHGDVPMA VEAMKAGAVD FIEKPFEDTV IIEAIERASE 

       130        140        150        160        170        180 
HLVAAEADVD DANDIRARLQ TLSERERQVL SAVVAGLPNK SIAYDLDISP RTVEVHRANV 

       190        200 
MAKMKAKSLP HLVRMALAGG FGPS

« Hide

References

« Hide 'large scale' references
[1]"Cascade regulation of nif gene expression in Rhizobium meliloti."
David M., Daveran M.-L., Batut J., Dedieu A., Domergue O., Ghai J., Hertig C., Boistard P., Kahn D.
Cell 54:671-683(1988) [PubMed: 2842062] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid."
Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. expand/collapse author list , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R.
Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed: 11474104] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[4]"fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti."
Batut J., Daveran-Mingot M.-L., David M., Jacobs J., Garnerone A.-M., Kahn D.
EMBO J. 8:1279-1286(1989) [PubMed: 2663474] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 200-204.
[5]"Modular structure of FixJ: homology of the transcriptional activator domain with the -35 binding domain of sigma factors."
Kahn D., Ditta G.S.
Mol. Microbiol. 5:987-997(1991) [PubMed: 1857213] [Abstract]
Cited for: REVIEW, MUTAGENESIS OF HTH REGION.
[6]"Structural transitions in the FixJ receiver domain."
Gouet P., Fabry B., Guillet V., Birck C., Mourey L., Kahn D., Samama J.-P.
Structure 7:1517-1526(1999) [PubMed: 10647182] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-126.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z21854 Genomic DNA. Translation: CAA79898.1.
AE006469 Genomic DNA. Translation: AAK65327.1.
X15079 Genomic DNA. Translation: CAA33182.1.
PIRB31227.
E95345.
RefSeqNP_435915.1. NC_003037.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5WX-ray2.30A/B/C3-124[»]
1DBWX-ray1.60A/B3-124[»]
1DCKX-ray2.00A/B3-124[»]
1DCMX-ray3.00A/B3-124[»]
1X3UNMR-A130-204[»]
ProteinModelPortalP10958.
SMRP10958. Positions 3-123, 129-204.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1235705.
GenomeReviewsGene locus RA0669 in contig AE006469_GR.
KEGGsme:SMa1227.
NMPDRfig|266834.1.peg.669.
PATRIC23627820. VBISinMel96828_0689.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753323.
OMASCANAFL.
ProtClustDBPRK09390.

Enzyme and pathway databases

BioCycSMEL266834:SMA1227-MONOMER.

Family and domain databases

InterProIPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KOK14987.
PfamPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSPR00038. HTHLUXR.
SMARTSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. CheY_like. 1 hit.
PROSITEPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIXJ_RHIME
AccessionPrimary (citable) accession number: P10958
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 25, 2012
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents