P10950 (ASMT_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylserotonin O-methyltransferase
EC=2.1.1.4

Alternative name(s):
Hydroxyindole O-methyltransferase
Short name=HIOMT

Gene names

Name:

ASMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin.
Catalytic activity	S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine.
Pathway	Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
Tissue specificity	Expressed in the pineal gland (at protein level). Not detectable in retina, nor in liver. Ref.1 Ref.3
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Biological process	Melatonin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	melatonin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetylserotonin O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Acetylserotonin O-methyltransferase		PRO_0000083980
Experimental info
	Sequence conflict	18	1	G → A in AAA30565. Ref.1
	Sequence conflict	58 – 121	64	QGTEL…WRHLA → GDRAATEHLCVPEAAASRRE GRKSCVCKHGARQHLPGERQ PQVPAGHAAVRGQDRLRLLA PPG in AAA30565. Ref.1
	Sequence conflict	315 – 345	31	AEYRA…VLARK → GRSTARSVGPAASETCGDGG RGEPTMLSWPGNQACSV in AAA30565. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10950 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: AA8EB84EB8D4A7E4	FASTA	345	37,924
	10 20 30 40 50 60 MCSQEGEGYS LLKEYANGFM VSQVLFAACE LGVFELLAEA LEPLDSAAVS SHLGSSPQGT 70 80 90 100 110 120 ELLLNTCVSL KLLQADVRGG KAVYANTELA STYLVRGSPR SQRDMLLYAG RTAYVCWRHL 130 140 150 160 170 180 AEAVREGRNQ YLKAFGIPSE ELFSAIYRSE DERLQFMQGL QDVWRLEGAT VLAAFDLSPF 190 200 210 220 230 240 PLICDLGGGS GALAKACVSL YPGCRAIVFD IPGVVQIAKR HFSASEDERI SFHEGDFFKD 250 260 270 280 290 300 ALPEADLYIL ARVLHDWTDA KCSHLLQRVY RACRTGGGIL VIESLLDTDG RGPLTTLLYS 310 320 330 340 LNMLVQTEGR ERTPAEYRAL LGPAGFRDVR CRRTGGTYDA VLARK

References

[1]	"Molecular cloning and nucleotide sequence of cDNA encoding hydroxyindole O-methyltransferase of bovine pineal glands." Ishida I., Obinata M., Deguchi T. J. Biol. Chem. 262:2895-2899(1987) [PubMed: 3818627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Pineal gland.
[2]	"Bovine hydroxyindole-O-methyltransferase. Significant sequence revision." Donohue S.J., Roseboom P.H., Klein D.C. J. Biol. Chem. 267:5184-5185(1992) [PubMed: 1544900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION.
[3]	"Human hydroxyindole-O-methyltransferase in pineal gland, retina and Y79 retinoblastoma cells." Bernard M., Donohue S.J., Klein D.C. Brain Res. 696:37-48(1995) [PubMed: 8574683] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02671 mRNA. Translation: AAA30565.1. M81862 mRNA. Translation: AAA30570.1.
IPI	IPI00691900.
PIR	A42106.
RefSeq	NP_803459.1. NM_177493.2.
UniGene	Bt.3930.
3D structure databases
ProteinModelPortal	P10950.
ModBase	Search...
Proteomic databases
PRIDE	P10950.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	281013.
KEGG	bta:281013.
Organism-specific databases
CTD	438.
Phylogenomic databases
HOVERGEN	HBG001526.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K00543.
Pfam	PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASMT_BOVIN

Accession

Primary (citable) accession number: P10950
Secondary accession number(s): Q28123

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program