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Protein

Acetylserotonin O-methyltransferase

Gene

ASMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).By similarity

Catalytic activityi

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetylserotonin O-methyltransferase (ASMT), Serotonin N-acetyltransferase (AANAT)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei164S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei210S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei252S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei255Proton donor/acceptorBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei306SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processMelatonin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylserotonin O-methyltransferase (EC:2.1.1.4)
Alternative name(s):
Hydroxyindole O-methyltransferase
Short name:
HIOMT
Gene namesi
Name:ASMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243914.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839801 – 345Acetylserotonin O-methyltransferaseAdd BLAST345

Proteomic databases

PaxDbiP10950.
PRIDEiP10950.

Expressioni

Tissue specificityi

Expressed in the pineal gland (at protein level). Not detectable in retina, nor in liver.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054648.

Structurei

3D structure databases

ProteinModelPortaliP10950.
SMRiP10950.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237S-adenosyl-L-methionine bindingPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
HOVERGENiHBG001526.
InParanoidiP10950.
KOiK00543.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR031725. ASMT_dimerisation.
IPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR029063. SAM-dependent_MTases.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF16864. Dimerisation2. 1 hit.
PF00891. Methyltransf_2. 1 hit.
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51683. SAM_OMT_II. 1 hit.

Sequencei

Sequence statusi: Complete.

P10950-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSQEGEGYS LLKEYANGFM VSQVLFAACE LGVFELLAEA LEPLDSAAVS
60 70 80 90 100
SHLGSSPQGT ELLLNTCVSL KLLQADVRGG KAVYANTELA STYLVRGSPR
110 120 130 140 150
SQRDMLLYAG RTAYVCWRHL AEAVREGRNQ YLKAFGIPSE ELFSAIYRSE
160 170 180 190 200
DERLQFMQGL QDVWRLEGAT VLAAFDLSPF PLICDLGGGS GALAKACVSL
210 220 230 240 250
YPGCRAIVFD IPGVVQIAKR HFSASEDERI SFHEGDFFKD ALPEADLYIL
260 270 280 290 300
ARVLHDWTDA KCSHLLQRVY RACRTGGGIL VIESLLDTDG RGPLTTLLYS
310 320 330 340
LNMLVQTEGR ERTPAEYRAL LGPAGFRDVR CRRTGGTYDA VLARK
Length:345
Mass (Da):37,924
Last modified:November 1, 1997 - v2
Checksum:iAA8EB84EB8D4A7E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18G → A in AAA30565 (PubMed:3818627).Curated1
Sequence conflicti58 – 121QGTEL…WRHLA → GDRAATEHLCVPEAAASRRE GRKSCVCKHGARQHLPGERQ PQVPAGHAAVRGQDRLRLLA PPG in AAA30565 (PubMed:3818627).CuratedAdd BLAST64
Sequence conflicti315 – 345AEYRA…VLARK → GRSTARSVGPAASETCGDGG RGEPTMLSWPGNQACSV in AAA30565 (PubMed:3818627).CuratedAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02671 mRNA. Translation: AAA30565.1.
M81862 mRNA. Translation: AAA30570.1.
PIRiA42106.
RefSeqiNP_803459.1. NM_177493.2.
UniGeneiBt.3930.

Genome annotation databases

GeneIDi281013.
KEGGibta:281013.

Similar proteinsi

Entry informationi

Entry nameiASMT_BOVIN
AccessioniPrimary (citable) accession number: P10950
Secondary accession number(s): Q28123
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families