P10936 (YES_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase Yes
EC=2.7.10.2

Alternative name(s):
p61-Yes

Gene names

Name:	yes1
Synonyms:	yes

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, differentiation, G2/M progression and cytokinesis By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Cell membrane By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytosol By similarity.
Post-translational modification	Autophosphorylated at Tyr-420 inducing activation By similarity. Palmitoylation at Cys-3 promotes membrane localization By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 537	536	Tyrosine-protein kinase Yes		PRO_0000088184
Regions
	Domain	85 – 146	62	SH3
	Domain	152 – 249	98	SH2
	Domain	271 – 524	254	Protein kinase
	Nucleotide binding	277 – 285	9	ATP By similarity
Sites
	Active site	390	1	Proton acceptor By similarity
	Binding site	299	1	ATP By similarity
Amino acid modifications
	Modified residue	420	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	531	1	Phosphotyrosine; by CSK By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine; in membrane form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10936 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FF26F615940AC31B	FASTA	537	60,358
	10 20 30 40 50 60 MGCIKSKEDK GPSIKYRTEP KPDPGSQYGA DPTQATQSPG IKGPAPNFNS HSMTPFGGSS 70 80 90 100 110 120 GITPFGGASS IFSPTPVPYP GGLTGGVTVF VALYDYEART TEDLSFRKGE RFQIINNTEG 130 140 150 160 170 180 DWWEARSIAT GKTGYIPSNY VAPADSIQAE EWYFGKMGRK DAERLLLNPG NQRGTFLVRE 190 200 210 220 230 240 SETTKGAYSL SIRDWDEVRG DNVKHYKIRK LDNGGYYITT RAQFESLQKL VKHYSEHADG 250 260 270 280 290 300 LCYRLTTVCP SVKPQTQGLA KDAWEIPRES LRLDVKLGQG CFGEVWIGTW NGTTKVAIKT 310 320 330 340 350 360 LKPGTMMPEA FLQEAQIMKK LRHDKLVPLY AVVSEEPIYI VTEYMIKGSL LDFLKEGNGK 370 380 390 400 410 420 YLKLPQLVDM AAQIADGMAY IERMNYIHRD LRAANILVGD NLVCKIADFG LARLIEDNEY 430 440 450 460 470 480 TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV AKGRVPYPGM VNREVLEQVE 490 500 510 520 530 RGYRMPCPQR CPESLHELMK LCWKKDPDER PTFEYIQSFL EDYFTATEPQ YQPGDNL

References

[1]	"The yes proto-oncogene is present in amphibians and contributes to the maternal RNA pool in the oocyte." Steele R.E., Irwin M.Y., Knudsen C.L., Collett J.W., Fero J.B. Oncogene Res. 4:223-233(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14377 mRNA. Translation: CAA32551.1.
PIR	A45501.
UniGene	Xl.58.
3D structure databases
ProteinModelPortal	P10936.
SMR	P10936. Positions 87-537.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Xenbase	XB-GENE-866413. yes1.
Phylogenomic databases
HOVERGEN	HBG008761.
Enzyme and pathway databases
BRENDA	2.7.10.2. 6726.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YES_XENLA

Accession

Primary (citable) accession number: P10936

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program