P10930 (VG12_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short tail fiber protein

Alternative name(s):
Protein Gp12
Short name=p12

Gene names

Name:

12

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural component of the short tail fiber. Secondary adhesin that binds irreversibly to the lipopolysaccharides component on the cell surface of Escherichia coli B strains during virus attachment. Ref.5
Subunit structure	Homotrimer.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 527

527

Short tail fiber protein

PRO_0000165002

Experimental info

Sequence conflict

33

1

Q → H in CAA29951. Ref.1

Sequence conflict

33

1

Q → H in AAD42417. Ref.2

Secondary structure

1

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527

Helix Strand Turn

Details...

Helix

335 – 340

6

Beta strand

346 – 353

8

Beta strand

359 – 364

6

Turn

369 – 371

3

Helix

373 – 378

6

Turn

379 – 383

5

Beta strand

387 – 391

5

Helix

407 – 410

4

Helix

412 – 414

3

Beta strand

415 – 417

3

Turn

425 – 428

4

Beta strand

454 – 456

3

Beta strand

465 – 467

3

Helix

493 – 495

3

Helix

498 – 500

3

Beta strand

508 – 510

3

Beta strand

520 – 524

5

Sequences

	Sequence		Length	Mass (Da)
	P10930 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: F86C7E65205486CC	FASTA	527	56,205
	10 20 30 40 50 60 MSNNTYQHVS NESRYVKFDP TDTNFPPEIT DVQAAIAAIS PAGVNGVPDA SSTTKGILFI 70 80 90 100 110 120 PTEQEVIDGT NNTKAVTPAT LATRLSYPNA TETVYGLTRY STNDEAIAGV NNESSITPAK 130 140 150 160 170 180 FTVALNNAFE TRVSTESSNG VIKISSLPQA LAGADDTTAM TPLKTQQLAI KLIAQIAPSE 190 200 210 220 230 240 TTATESDQGV VQLATVAQVR QGTLREGYAI SPYTFMNSSS TEEYKGVIKL GTQSEVNSNN 250 260 270 280 290 300 ASVAVTGATL NGRGSTTSMR GVVKLTTTAG SQSGGDASSA LAWNADVIQQ RGGQIIYGTL 310 320 330 340 350 360 RIEDTFTIAN GGANITGTVR MTGGYIQGNR IVTQNEIDRT IPVGAIMMWA ADSLPSDAWR 370 380 390 400 410 420 FCHGGTVSAS DCPLYASRIG TRYGGNPSNP GLPDMRGLFV RGSGRGSHLT NPNVNGNDQF 430 440 450 460 470 480 GKPRLGVGCT GGYVGEVQIQ QMSYHKHAGG FGEHDDLGAF GNTRRSNFVG TRKGLDWDNR 490 500 510 520 SYFTNDGYEI DPESQRNSKY TLNRPELIGN ETRPWNISLN YIIKVKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide and deduced amino acid sequence of bacteriophage T4 gene 12." Selivanov N.A., Prilipov A.G., Mesyanzhinov V.V. Nucleic Acids Res. 16:2334-2334(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D.
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Using transposon Tn5 insertions to sequence bacteriophage T4 gene 11." Barrett B.K., Berget P.B. DNA 8:287-295(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57. Strain: D.
[4]	"Nucleotide sequences of bacteriophage T4 genes 9, 10 and 11." Prilipov A.G., Selivanov N.A., Efimov V.P., Marusich E.I., Mesyanzhinov V.V. Nucleic Acids Res. 17:3303-3303(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4. Strain: D.
[5]	"Homotrimeric, beta-stranded viral adhesins and tail proteins." Weigele P.R., Scanlon E., King J. J. Bacteriol. 185:4022-4030(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Crystal structure of a heat and protease-stable part of the bacteriophage T4 short tail fibre." van Raaij M.J., Schoehn G., Burda M.R., Miller S. J. Mol. Biol. 314:1137-1146(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 250-527.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X06792 Genomic DNA. Translation: CAA29951.1.
AF158101 Genomic DNA. Translation: AAD42417.1.
M26253 Genomic DNA. Translation: AAA32495.1.
X14192 Genomic DNA. Translation: CAA32398.1.

PIR

C32479.
GIBPT4. S01889.

RefSeq

NP_049770.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H6W	X-ray	1.90	A	85-396	[»]
			B	518-527	[»]
1OCY	X-ray	1.50	A	332-527	[»]
1PDI	electron microscopy	12.00	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R	250-527	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258789.

Phylogenomic databases

ProtClustDB

CLSP2343202.

Family and domain databases

Gene3D

2.160.30.10. 1 hit.
3.90.1340.10. 1 hit.

InterPro

IPR015173. Phage_T4_Gp12.
IPR023304. Phage_T4_Gp12_dom2.
IPR011083. phage_tail_collar_dom.
[Graphical view]

Pfam

PF07484. Collar. 1 hit.
PF09089. gp12-short_mid. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10930.

Entry information

Entry name

VG12_BPT4

Accession

Primary (citable) accession number: P10930

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program