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Protein

Baseplate wedge protein gp10

Gene

10

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:16554069). During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure. At this point, gp10 rotates and acts as a lever that unfolds the short tail fibers, which then interact with host cell surface receptors. Involved in the tail assembly.1 Publication1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral tail assembly, Virus exit from host cell

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate wedge protein gp10Curated
Alternative name(s):
Gene product 10Curated
Short name:
gp10
Gene namesi
Name:10
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Baseplate wedge protein gp10PRO_0000165000Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.Curated

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer (PubMed:19896486). Part of the baseplate macromolecular complex which consists of gp5, gp26, gp27, gp28, gp29 (hub); gp6, gp7, gp8, gp10, gp11, gp25, gp53 (wedge); gp9, gp12, gp48 and gp54.1 Publication1 Publication

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi414 – 4163Combined sources
Beta strandi420 – 4234Combined sources
Beta strandi425 – 4273Combined sources
Beta strandi432 – 4354Combined sources
Beta strandi439 – 4413Combined sources
Helixi446 – 4538Combined sources
Beta strandi459 – 4646Combined sources
Helixi470 – 4734Combined sources
Beta strandi477 – 4804Combined sources
Turni497 – 4993Combined sources
Beta strandi500 – 5067Combined sources
Beta strandi512 – 5165Combined sources
Beta strandi523 – 5253Combined sources
Helixi529 – 5313Combined sources
Beta strandi596 – 6027Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FKKX-ray1.20A397-602[»]
2FL8electron microscopy1.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-602[»]
2FL9electron microscopy17.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-602[»]
ProteinModelPortaliP10928.
SMRiP10928. Positions 406-602.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10928.

Family & Domainsi

Family and domain databases

InterProiIPR008987. Baseplate_struct_prot_Gp9/10.
[Graphical view]
PfamiPF07880. T4_gp9_10. 1 hit.
[Graphical view]
SUPFAMiSSF50017. SSF50017. 1 hit.

Sequencei

Sequence statusi: Complete.

P10928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQNINIGNV VDDGTGDYLR KGGIKINENF DELYYELGDG DVPYSAGAWK
60 70 80 90 100
TYNASSGQTL TAEWGKSYAI NTSSGRVTIN LPKGTVNDYN KVIRARDVFA
110 120 130 140 150
TWNVNPVTLV AASGDTIKGS AVPVEINVRF SDLELVYCAP GRWEYVKNKQ
160 170 180 190 200
IDKITSSDIS NVARKEFLVE VQGQTDFLDV FRGTSYNVNN IRVKHRGNEL
210 220 230 240 250
YYGDVFSENS DFGSPGENEG ELVPLDGFNI RLRQPCNIGD TVQIETFMDG
260 270 280 290 300
VSQWRSSYTR RQIRLLDSKL TSKTSLEGSI YVTDLSTMKS IPFSAFGLIP
310 320 330 340 350
GEPINPNSLE VRFNGILQEL AGTVGMPLFH CVGADSDDEV ECSVLGGTWE
360 370 380 390 400
QSHTDYSVET DENGIPEILH FDSVFEHGDI INITWFNNDL GTLLTKDEII
410 420 430 440 450
DETDNLYVSQ GPGVDISGDV NLTDFDKIGW PNVEAVQSYQ RAFNAVSNIF
460 470 480 490 500
DTIYPIGTIY ENAVNPNNPV TYMGFGSWKL FGQGKVLVGW NEDISDPNFA
510 520 530 540 550
LNNNDLDSGG NPSHTAGGTG GSTSVTLENA NLPATETDEE VLIVDENGSV
560 570 580 590 600
IVGGCQYDPD ESGPIYTKYR EAKASTNSTH TPPTSITNIQ PYITVYRWIR

IA
Length:602
Mass (Da):66,232
Last modified:July 1, 1989 - v1
Checksum:i1159B8C7EBB8EF54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14192 Genomic DNA. Translation: CAA32396.1.
AF158101 Genomic DNA. Translation: AAD42415.1.
M26253 Genomic DNA. Translation: AAA32493.1.
PIRiS04083. GEBPT4.
RefSeqiNP_049768.1. NC_000866.4.

Genome annotation databases

GeneIDi1258808.
KEGGivg:1258808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14192 Genomic DNA. Translation: CAA32396.1.
AF158101 Genomic DNA. Translation: AAD42415.1.
M26253 Genomic DNA. Translation: AAA32493.1.
PIRiS04083. GEBPT4.
RefSeqiNP_049768.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FKKX-ray1.20A397-602[»]
2FL8electron microscopy1.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-602[»]
2FL9electron microscopy17.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-602[»]
ProteinModelPortaliP10928.
SMRiP10928. Positions 406-602.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258808.
KEGGivg:1258808.

Miscellaneous databases

EvolutionaryTraceiP10928.

Family and domain databases

InterProiIPR008987. Baseplate_struct_prot_Gp9/10.
[Graphical view]
PfamiPF07880. T4_gp9_10. 1 hit.
[Graphical view]
SUPFAMiSSF50017. SSF50017. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Using transposon Tn5 insertions to sequence bacteriophage T4 gene 11."
    Barrett B.K., Berget P.B.
    DNA 8:287-295(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 535-602.
    Strain: D.
  4. "Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking."
    Watts N.R., Coombs D.H.
    J. Virol. 64:143-154(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: REVIEW.
  6. Cited for: REVIEW ON FUNCTION.
  7. "The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro."
    Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.
    J. Mol. Biol. 395:349-360(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host."
    Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V., Rossmann M.G.
    Cell 118:419-429(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL, SUBCELLULAR LOCATION.
  9. "Evolution of bacteriophage tails: Structure of T4 gene product 10."
    Leiman P.G., Shneider M.M., Mesyanzhinov V.V., Rossmann M.G.
    J. Mol. Biol. 358:912-921(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 397-602, FUNCTION.

Entry informationi

Entry nameiBP10_BPT4
AccessioniPrimary (citable) accession number: P10928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 27, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The predicted MW of Gp10 is about 22 kDa smaller than in mature phage particles. Therefore Gp10 may exist as a hybrid protein consisting of two neighboring gene products.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.