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Protein

Baseplate wedge protein gp9

Gene

9

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate protein that connects the long tail fibers to the baseplate and probably triggers the tail contraction after virus attachment to a host cell (PubMed:10545330). Involved in the tail assembly (PubMed:21129200).1 Publication1 Publication

GO - Biological processi

  1. viral life cycle Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Viral tail assembly, Virus exit from host cell

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate wedge protein gp9Curated
Alternative name(s):
Gene product 9Curated
Short name:
gp9
Gene namesi
Name:9
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087 Componenti: Genome

Subcellular locationi

  1. Virion 2 Publications

  2. Note: Present in 18 copies in the baseplate.1 Publication

GO - Cellular componenti

  1. virion Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Baseplate wedge protein gp9PRO_0000164999Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.Curated

Interactioni

Subunit structurei

Homotrimer. Part of the baseplate macromolecular complex which consists of gp5, gp26, gp27, gp28, gp29 (hub); gp6, gp7, gp8, gp10, gp11, gp25, gp53 (wedge); gp9, gp12, gp48 and gp54.3 Publications

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Helixi20 – 3920Combined sources
Helixi45 – 495Combined sources
Beta strandi50 – 534Combined sources
Helixi74 – 763Combined sources
Beta strandi84 – 863Combined sources
Beta strandi90 – 923Combined sources
Beta strandi94 – 985Combined sources
Beta strandi106 – 1105Combined sources
Beta strandi118 – 1203Combined sources
Beta strandi122 – 1309Combined sources
Beta strandi134 – 1396Combined sources
Beta strandi144 – 1529Combined sources
Beta strandi154 – 1563Combined sources
Beta strandi159 – 16911Combined sources
Beta strandi176 – 1805Combined sources
Beta strandi185 – 1939Combined sources
Turni194 – 1963Combined sources
Beta strandi198 – 20811Combined sources
Beta strandi214 – 22411Combined sources
Turni225 – 2284Combined sources
Beta strandi229 – 24113Combined sources
Beta strandi243 – 2475Combined sources
Beta strandi251 – 2577Combined sources
Beta strandi260 – 2667Combined sources
Beta strandi268 – 28215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PDPelectron microscopy12.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-288[»]
1QEXX-ray2.30A/B1-288[»]
1S2EX-ray2.30A/B1-288[»]
1TJAelectron microscopy16.00C/D/E1-288[»]
1ZKUelectron microscopy15.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-288[»]
ProteinModelPortaliP10927.
SMRiP10927. Positions 1-288.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10927.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili20 – 80611 PublicationAdd
BLAST

Domaini

The C-terminus is bound to the baseplate, and the N-terminus coiled-coil domain is associated with the long tail fibers.

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di2.60.120.640. 1 hit.
2.60.40.1680. 1 hit.
InterProiIPR008987. Baseplate_struct_prot_Gp9/10.
IPR027412. Gp9_C_dom.
IPR027411. Gp9_mid_dom.
[Graphical view]
PfamiPF07880. T4_gp9_10. 1 hit.
[Graphical view]
SUPFAMiSSF50017. SSF50017. 1 hit.

Sequencei

Sequence statusi: Complete.

P10927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIQEPKKLI DTGEIGNAST GDILFDGGNK INSDFNAIYN AFGDQRKMAV
60 70 80 90 100
ANGTGADGQI IHATGYYQKH SITEYATPVK VGTRHDIDTS TVGVKVIIER
110 120 130 140 150
GELGDCVEFI NSNGSISVTN PLTIQAIDSI KGVSGNLVVT SPYSKVTLRC
160 170 180 190 200
ISSDNSTSVW NYSIESMFGQ KESPAEGTWN ISTSGSVDIP LFHRTEYNMA
210 220 230 240 250
KLLVTCQSVD GRKIKTAEIN ILVDTVNSEV ISSEYAVMRV GNETEEDEIA
260 270 280
NIAFSIKENY VTATISSSTV GMRAAVKVIA TQKIGVAQ
Length:288
Mass (Da):30,997
Last modified:July 1, 1989 - v1
Checksum:i8028FCEBA25BB760
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14192 Genomic DNA. Translation: CAA32395.1.
AF158101 Genomic DNA. Translation: AAD42520.1.
PIRiS04082. GNBPT4.
RefSeqiNP_049767.1. NC_000866.4.

Genome annotation databases

GeneIDi1258617.
KEGGivg:1258617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14192 Genomic DNA. Translation: CAA32395.1.
AF158101 Genomic DNA. Translation: AAD42520.1.
PIRiS04082. GNBPT4.
RefSeqiNP_049767.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PDPelectron microscopy12.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-288[»]
1QEXX-ray2.30A/B1-288[»]
1S2EX-ray2.30A/B1-288[»]
1TJAelectron microscopy16.00C/D/E1-288[»]
1ZKUelectron microscopy15.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-288[»]
ProteinModelPortaliP10927.
SMRiP10927. Positions 1-288.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258617.
KEGGivg:1258617.

Miscellaneous databases

EvolutionaryTraceiP10927.

Family and domain databases

Gene3Di2.60.120.640. 1 hit.
2.60.40.1680. 1 hit.
InterProiIPR008987. Baseplate_struct_prot_Gp9/10.
IPR027412. Gp9_C_dom.
IPR027411. Gp9_mid_dom.
[Graphical view]
PfamiPF07880. T4_gp9_10. 1 hit.
[Graphical view]
SUPFAMiSSF50017. SSF50017. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking."
    Watts N.R., Coombs D.H.
    J. Virol. 64:143-154(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.
  4. Cited for: REVIEW.
  5. Cited for: REVIEW ON FUNCTION.
  6. "The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro."
    Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.
    J. Mol. Biol. 395:349-360(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "The structure of bacteriophage T4 gene product 9: the trigger for tail contraction."
    Kostyuchenko V.A., Navruzbekov G.A., Kurochkina L.P., Strelkov S.V., Mesyanzhinov V.V., Rossmann M.G.
    Structure 7:1213-1222(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, SUBUNIT, DOMAIN, COILED COIL.
  8. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host."
    Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V., Rossmann M.G.
    Cell 118:419-429(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBP09_BPT4
AccessioniPrimary (citable) accession number: P10927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 29, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.