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Protein

Osteopontin

Gene

Spp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB
  • extracellular matrix binding Source: MGI

GO - Biological processi

  • biomineral tissue development Source: UniProtKB-KW
  • cell adhesion Source: UniProtKB-KW
  • cellular response to fluid shear stress Source: MGI
  • negative regulation of apoptotic process Source: UniProtKB
  • neutrophil chemotaxis Source: MGI
  • osteoblast differentiation Source: GO_Central
  • positive regulation of bone resorption Source: GO_Central
  • positive regulation of cell-substrate adhesion Source: MGI
  • response to vitamin D Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-186797. Signaling by PDGF.
R-MMU-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
2AR
Bone sialoprotein 1
Calcium oxalate crystal growth inhibitor protein
Early T-lymphocyte activation 1 protein
Minopontin
Secreted phosphoprotein 1
Short name:
SPP-1
Gene namesi
Name:Spp1
Synonyms:Eta-1, Op, Spp-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:98389. Spp1.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular region Source: UniProtKB
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000002032217 – 294OsteopontinAdd BLAST278

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei27PhosphoserineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1
Modified residuei80PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei118PhosphoserineBy similarity1
Glycosylationi123O-linked (GalNAc...)By similarity1
Glycosylationi132O-linked (GalNAc...)By similarity1
Glycosylationi137O-linked (GalNAc...)By similarity1
Modified residuei170PhosphothreonineBy similarity1
Modified residuei175PhosphothreonineBy similarity1
Modified residuei176PhosphoserineBy similarity1
Modified residuei180PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1
Modified residuei228PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei234PhosphoserineCombined sources1
Modified residuei238PhosphoserineBy similarity1
Modified residuei243PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei250PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei260PhosphoserineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei283PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei291PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity
N- and O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP10923.
PaxDbiP10923.
PeptideAtlasiP10923.
PRIDEiP10923.

PTM databases

iPTMnetiP10923.
PhosphoSitePlusiP10923.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029304.
CleanExiMM_SPP1.
ExpressionAtlasiP10923. baseline and differential.
GenevisibleiP10923. MM.

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB

Protein-protein interaction databases

BioGridi203467. 3 interactors.
IntActiP10923. 1 interactor.
STRINGi10090.ENSMUSP00000084043.

Structurei

3D structure databases

ProteinModelPortaliP10923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi144 – 146Cell attachment site3

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP10923.
KOiK06250.
PhylomeDBiP10923.
TreeFamiTF350201.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 1 hit.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK
60 70 80 90 100
QNLLAPQNAV SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES
110 120 130 140 150
EDSVDSDESD ESHHSDESDE TVTASTQADT FTPIVPTVDV PNGRGDSLAY
160 170 180 190 200
GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS GESKESLDVI PVAQLLSMPS
210 220 230 240 250
DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV IDSQASSKAS
260 270 280 290
LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN
Length:294
Mass (Da):32,459
Last modified:July 1, 1989 - v1
Checksum:i9D5F32D67ABC53EA
GO

Sequence cautioni

The sequence AAH14284 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43L → P in CAA34276 (PubMed:2787378).Curated1
Sequence conflicti99E → G in AAA57265 (PubMed:2722855).Curated1
Sequence conflicti100S → N in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti122V → F in CAA32165 (PubMed:2726465).Curated1
Sequence conflicti122V → F in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti142N → D in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti171D → Y in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti188D → N in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti207K → R in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti224R → S in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti232Q → H in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti277Y → H in AAH14284 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04806 mRNA. Translation: AAA57265.1.
X16151 mRNA. Translation: CAA34276.1.
X13986 mRNA. Translation: CAA32165.1.
X51834 Genomic DNA. Translation: CAA36132.1.
BC014284 mRNA. Translation: AAH14284.1. Different initiation.
BC057858 mRNA. Translation: AAH57858.1.
CCDSiCCDS19486.1.
PIRiA37818.
RefSeqiNP_001191162.1. NM_001204233.1.
NP_033289.2. NM_009263.3.
UniGeneiMm.288474.

Genome annotation databases

EnsembliENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304.
ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304.
ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304.
GeneIDi20750.
KEGGimmu:20750.
UCSCiuc008yki.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04806 mRNA. Translation: AAA57265.1.
X16151 mRNA. Translation: CAA34276.1.
X13986 mRNA. Translation: CAA32165.1.
X51834 Genomic DNA. Translation: CAA36132.1.
BC014284 mRNA. Translation: AAH14284.1. Different initiation.
BC057858 mRNA. Translation: AAH57858.1.
CCDSiCCDS19486.1.
PIRiA37818.
RefSeqiNP_001191162.1. NM_001204233.1.
NP_033289.2. NM_009263.3.
UniGeneiMm.288474.

3D structure databases

ProteinModelPortaliP10923.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203467. 3 interactors.
IntActiP10923. 1 interactor.
STRINGi10090.ENSMUSP00000084043.

PTM databases

iPTMnetiP10923.
PhosphoSitePlusiP10923.

Proteomic databases

EPDiP10923.
PaxDbiP10923.
PeptideAtlasiP10923.
PRIDEiP10923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304.
ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304.
ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304.
GeneIDi20750.
KEGGimmu:20750.
UCSCiuc008yki.2. mouse.

Organism-specific databases

CTDi6696.
MGIiMGI:98389. Spp1.

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP10923.
KOiK06250.
PhylomeDBiP10923.
TreeFamiTF350201.

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-186797. Signaling by PDGF.
R-MMU-216083. Integrin cell surface interactions.

Miscellaneous databases

ChiTaRSiSpp1. mouse.
PROiP10923.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029304.
CleanExiMM_SPP1.
ExpressionAtlasiP10923. baseline and differential.
GenevisibleiP10923. MM.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 1 hit.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSTP_MOUSE
AccessioniPrimary (citable) accession number: P10923
Secondary accession number(s): P19008, Q91VH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.