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P10923 (OSTP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Osteopontin
Alternative name(s):
2AR
Bone sialoprotein 1
Calcium oxalate crystal growth inhibitor protein
Early T-lymphocyte activation 1 protein
Minopontin
Secreted phosphoprotein 1
Short name=SPP-1
Gene names
Name:Spp1
Synonyms:Eta-1, Op, Spp-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Ref.7 Ref.8

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. Ref.7 Ref.8

Subunit structure

Ligand for integrin alpha-V/beta-3.

Subcellular location

Secreted.

Post-translational modification

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif By similarity.

N- and O-glycosylated.

Sequence similarities

Belongs to the osteopontin family.

Sequence caution

The sequence AAH14284.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 294278Osteopontin
PRO_0000020322

Regions

Motif144 – 1463Cell attachment site

Amino acid modifications

Modified residue241Phosphoserine By similarity
Modified residue261Phosphoserine By similarity
Modified residue271Phosphoserine By similarity
Modified residue611Phosphoserine By similarity
Modified residue621Phosphoserine By similarity
Modified residue751Phosphoserine By similarity
Modified residue771Phosphoserine By similarity
Modified residue801Phosphoserine By similarity
Modified residue1061Phosphoserine By similarity
Modified residue1091Phosphoserine By similarity
Modified residue1121Phosphoserine By similarity
Modified residue1151Phosphoserine By similarity
Modified residue1181Phosphoserine By similarity
Modified residue1701Phosphothreonine By similarity
Modified residue1761Phosphoserine By similarity
Modified residue1801Phosphoserine By similarity
Modified residue2001Phosphoserine By similarity
Modified residue2091Phosphoserine By similarity
Modified residue2131Phosphoserine By similarity
Modified residue2191Phosphoserine By similarity
Modified residue2341Phosphoserine By similarity
Modified residue2431Phosphoserine By similarity
Modified residue2471Phosphoserine By similarity
Modified residue2501Phosphoserine By similarity
Modified residue2551Phosphoserine By similarity
Modified residue2601Phosphoserine By similarity
Modified residue2711Phosphoserine By similarity
Modified residue2831Phosphoserine Ref.9 Ref.10
Modified residue2881Phosphoserine Ref.9
Modified residue2901Phosphoserine Ref.9 Ref.10
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation1231O-linked (GalNAc...) By similarity
Glycosylation1321O-linked (GalNAc...) By similarity
Glycosylation1371O-linked (GalNAc...) By similarity

Experimental info

Sequence conflict431L → P in CAA34276. Ref.2
Sequence conflict991E → G in AAA57265. Ref.1
Sequence conflict1001S → N in AAH14284. Ref.5
Sequence conflict1221V → F in CAA32165. Ref.3
Sequence conflict1221V → F in AAH14284. Ref.5
Sequence conflict1421N → D in AAH14284. Ref.5
Sequence conflict1711D → Y in AAH14284. Ref.5
Sequence conflict1881D → N in AAH14284. Ref.5
Sequence conflict2071K → R in AAH14284. Ref.5
Sequence conflict2241R → S in AAH14284. Ref.5
Sequence conflict2321Q → H in AAH14284. Ref.5
Sequence conflict2771Y → H in AAH14284. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P10923 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 9D5F32D67ABC53EA

FASTA29432,459
        10         20         30         40         50         60 
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK QNLLAPQNAV 

        70         80         90        100        110        120 
SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES EDSVDSDESD ESHHSDESDE 

       130        140        150        160        170        180 
TVTASTQADT FTPIVPTVDV PNGRGDSLAY GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS 

       190        200        210        220        230        240 
GESKESLDVI PVAQLLSMPS DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV 

       250        260        270        280        290 
IDSQASSKAS LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN 

« Hide

References

« Hide 'large scale' references
[1]"Osteopontin, a transformation-associated cell adhesion phosphoprotein, is induced by 12-O-tetradecanoylphorbol 13-acetate in mouse epidermis."
Craig A.M., Smith J.H., Denhardt D.T.
J. Biol. Chem. 264:9682-9689(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural and functional studies of the early T lymphocyte activation 1 (Eta-1) gene. Definition of a novel T cell-dependent response associated with genetic resistance to bacterial infection."
Patarca R., Freeman G.J., Singh R.P., Wei F.-Y., Durfee T., Blattner F., Regnier D.C., Kozak C.A., Mock B.A., Morse H.C. III, Jerrells T.R., Cantor H.
J. Exp. Med. 170:145-161(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Nucleotide sequence of cDNA for mouse osteopontin-like protein."
Miyazaki Y., Setoguchi M., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S.
Nucleic Acids Res. 17:3298-3298(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.
[4]"The mouse osteopontin gene. Expression in monocytic lineages and complete nucleotide sequence."
Miyazaki Y., Setoguchi M., Yoshida S.Y., Akizuki S., Yamamoto S.
J. Biol. Chem. 265:14432-14438(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Liver.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland.
[6]"The calcium oxalate crystal growth inhibitor protein produced by mouse kidney cortical cells in culture is osteopontin."
Worcester E.M., Blumenthal S.S., Beshensky A.M., Lewand D.L.
J. Bone Miner. Res. 7:1029-1036(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 17-37.
Tissue: Kidney.
[7]"Definition of a specific interaction between the early T lymphocyte activation 1 (Eta-1) protein and murine macrophages in vitro and its effect upon macrophages in vivo."
Singh R.P., Patarca R., Schwartz J., Singh P., Cantor H.
J. Exp. Med. 171:1931-1942(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 158-176, FUNCTION.
[8]"Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity."
Ashkar S., Weber G.F., Panoutsakopoulou V., Sanchirico M.E., Jansson M., Zawaideh S., Rittling S.R., Denhardt D.T., Glimcher M.J., Cantor H.
Science 287:860-864(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283; SER-288 AND SER-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[10]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283 AND SER-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04806 mRNA. Translation: AAA57265.1.
X16151 mRNA. Translation: CAA34276.1.
X13986 mRNA. Translation: CAA32165.1.
X51834 Genomic DNA. Translation: CAA36132.1.
BC014284 mRNA. Translation: AAH14284.1. Different initiation.
BC057858 mRNA. Translation: AAH57858.1.
PIRA37818.
RefSeqNP_001191162.1. NM_001204233.1.
NP_033289.2. NM_009263.3.
UniGeneMm.288474.

3D structure databases

ProteinModelPortalP10923.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203467. 1 interaction.

PTM databases

PhosphoSiteP10923.

Proteomic databases

PaxDbP10923.
PRIDEP10923.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304.
ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304.
ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304.
GeneID20750.
KEGGmmu:20750.
UCSCuc008yki.2. mouse.

Organism-specific databases

CTD6696.
MGIMGI:98389. Spp1.

Phylogenomic databases

eggNOGNOG73598.
HOGENOMHOG000059656.
HOVERGENHBG001731.
KOK06250.
PhylomeDBP10923.
TreeFamTF350201.

Gene expression databases

ArrayExpressP10923.
BgeeP10923.
CleanExMM_SPP1.
GenevestigatorP10923.

Family and domain databases

InterProIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERPTHR10607. PTHR10607. 1 hit.
PfamPF00865. Osteopontin. 1 hit.
[Graphical view]
PRINTSPR00216. OSTEOPONTIN.
SMARTSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPP1. mouse.
NextBio299411.
PROP10923.
SOURCESearch...

Entry information

Entry nameOSTP_MOUSE
AccessionPrimary (citable) accession number: P10923
Secondary accession number(s): P19008, Q91VH4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot