P10915 (HPLN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan and proteoglycan link protein 1

Alternative name(s):
Cartilage-linking protein 1
Short name=Cartilage-link protein
Proteoglycan link protein

Gene names

Name:	HAPLN1
Synonyms:	CRTL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Widely expressed. Weakly expressed in the brain. Ref.8 Ref.9
Sequence similarities	Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat
Ligand	Hyaluronic acid
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular_component	proteinaceous extracellular matrix Traceable author statement Ref.3. Source: ProtInc
Molecular_function	hyaluronic acid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 15	15			PRO_0000013177
	Chain	16 – 354	339	Hyaluronan and proteoglycan link protein 1		PRO_0000013178
Regions
	Domain	38 – 152	115	Ig-like V-type
	Domain	159 – 254	96	Link 1
	Domain	259 – 351	93	Link 2
Amino acid modifications
	Glycosylation	21	1	N-linked (GlcNAc...) Potential
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Disulfide bond	61 ↔ 139		By similarity
	Disulfide bond	181 ↔ 252		By similarity
	Disulfide bond	205 ↔ 226		By similarity
	Disulfide bond	279 ↔ 349		By similarity
	Disulfide bond	304 ↔ 325		By similarity
Natural variations
	Natural variant	281	1	N → S. Corresponds to variant rs6864342 [ dbSNP | Ensembl ].		VAR_049316
	Natural variant	333	1	R → H in a colorectal cancer sample; somatic mutation. Ref.10		VAR_036168
Experimental info
	Sequence conflict	274	1	E → V in AAH57808. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P10915 [UniParc]. Last modified April 1, 1990. Version 2. Checksum: 315C96EC3AC2626A	FASTA	354	40,166
	10 20 30 40 50 60 MKSLLLLVLI SICWADHLSD NYTLDHDRAI HIQAENGPHL LVEAEQAKVF SHRGGNVTLP 70 80 90 100 110 120 CKFYRDPTAF GSGIHKIRIK WTKLTSDYLK EVDVFVSMGY HKKTYGGYQG RVFLKGGSDS 130 140 150 160 170 180 DASLVITDLT LEDYGRYKCE VIEGLEDDTV VVALDLQGVV FPYFPRLGRY NLNFHEAQQA 190 200 210 220 230 240 CLDQDAVIAS FDQLYDAWRG GLDWCNAGWL SDGSVQYPIT KPREPCGGQN TVPGVRNYGF 250 260 270 280 290 300 WDKDKSRYDV FCFTSNFNGR FYYLIHPTKL TYDEAVQACL NDGAQIAKVG QIFAAWKILG 310 320 330 340 350 YDRCDAGWLA DGSVRYPISR PRRRCSPTEA AVRFVGFPDK KHKLYGVYCF RAYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of human cartilage link protein." Dudhia J., Hardingham T.E. Nucleic Acids Res. 18:1292-1292(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Articular chondrocyte.
[2]	Erratum Dudhia J., Hardingham T.E. Nucleic Acids Res. 18:2214-2214(1990) [PubMed] [Europe PMC] [Abstract]
[3]	"Complete amino acid sequence of human cartilage link protein (CRTL1) deduced from cDNA clones and chromosomal assignment of the gene." Osborne-Lawrence S.L., Sinclair A.K., Hicks R.C., Lacey S.W., Eddy R.L. Jr., Byers M.G., Shows T.B., Duby A.D. Genomics 8:562-567(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of the promoter for the rat and human link protein gene." Rhodes C., Savagner P., Line S., Sasaki M., Chirigos M., Doege K., Yamada Y. Nucleic Acids Res. 19:1933-1939(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[8]	"The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain." Hirakawa S., Oohashi T., Su W.-D., Yoshioka H., Murakami T., Arata J., Ninomiya Y. Biochem. Biophys. Res. Commun. 276:982-989(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"A hyaluronan binding link protein gene family whose members are physically linked adjacent to chondroitin sulfate proteoglycan core protein genes: the missing links." Spicer A.P., Joo A., Bowling R.A. Jr. J. Biol. Chem. 278:21083-21091(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-333.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17405 mRNA. Translation: CAA35462.1. U43328 mRNA. Translation: AAA85216.1. AK313713 mRNA. Translation: BAG36456.1. CH471084 Genomic DNA. Translation: EAW95912.1. BC057808 mRNA. Translation: AAH57808.1.
IPI	IPI00023601.
PIR	LKHU. S14914.
RefSeq	NP_001875.1. NM_001884.3.
UniGene	Hs.2799.
3D structure databases
ProteinModelPortal	P10915.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274341.
PTM databases
PhosphoSite	P10915.
Polymorphism databases
DMDM	130310.
Proteomic databases
PaxDb	P10915.
PRIDE	P10915.
Protocols and materials databases
DNASU	1404.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274341; ENSP00000274341; ENSG00000145681.
GeneID	1404.
KEGG	hsa:1404.
UCSC	uc003kim.3. human.
Organism-specific databases
CTD	1404.
GeneCards	GC05M082933.
HGNC	HGNC:2380. HAPLN1.
HPA	HPA019105. HPA019482.
MIM	115435. gene.
neXtProt	NX_P10915.
PharmGKB	PA26901.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145467.
HOGENOM	HOG000234353.
HOVERGEN	HBG051922.
InParanoid	P10915.
KO	K06848.
OMA	LISICWA.
OrthoDB	EOG4MKNGD.
PhylomeDB	P10915.
Gene expression databases
ArrayExpress	P10915.
Bgee	P10915.
CleanEx	HS_HAPLN1.
Genevestigator	P10915.
GermOnline	ENSG00000145681. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.10.100.10. 2 hits.
InterPro	IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013106. Ig_V-set. IPR003596. Ig_V-set_subgr. IPR000538. Link. [Graphical view]
Pfam	PF07686. V-set. 1 hit. PF00193. Xlink. 2 hits. [Graphical view]
PRINTS	PR01265. LINKMODULE.
SMART	SM00406. IGv. 1 hit. SM00445. LINK. 2 hits. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 2 hits.
PROSITE	PS50835. IG_LIKE. 1 hit. PS01241. LINK_1. 2 hits. PS50963. LINK_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1404.
NextBio	5743.
PMAP-CutDB	P10915.
SOURCE	Search...

Entry information

Entry name

HPLN1_HUMAN

Accession

Primary (citable) accession number: P10915
Secondary accession number(s): B2R9A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.