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P10915 (HPLN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronan and proteoglycan link protein 1
Alternative name(s):
Cartilage-linking protein 1
Short name=Cartilage-link protein
Proteoglycan link protein
Gene names
Name:HAPLN1
Synonyms:CRTL1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

Widely expressed. Weakly expressed in the brain. Ref.8 Ref.9

Sequence similarities

Belongs to the HAPLN family.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Contains 2 Link domains.

Ontologies

Keywords
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Repeat
   LigandHyaluronic acid
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: InterPro

extracellular matrix organization

Traceable author statement. Source: Reactome

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

proteinaceous extracellular matrix

Traceable author statement Ref.3. Source: ProtInc

   Molecular_functionhyaluronic acid binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1515
PRO_0000013177
Chain16 – 354339Hyaluronan and proteoglycan link protein 1
PRO_0000013178

Regions

Domain38 – 152115Ig-like V-type
Domain159 – 25496Link 1
Domain259 – 35193Link 2

Amino acid modifications

Glycosylation211N-linked (GlcNAc...) Potential
Glycosylation561N-linked (GlcNAc...) Potential
Disulfide bond61 ↔ 139 By similarity
Disulfide bond181 ↔ 252 By similarity
Disulfide bond205 ↔ 226 By similarity
Disulfide bond279 ↔ 349 By similarity
Disulfide bond304 ↔ 325 By similarity

Natural variations

Natural variant2811N → S.
Corresponds to variant rs6864342 [ dbSNP | Ensembl ].
VAR_049316
Natural variant3331R → H in a colorectal cancer sample; somatic mutation. Ref.10
VAR_036168

Experimental info

Sequence conflict2741E → V in AAH57808. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P10915 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 315C96EC3AC2626A

FASTA35440,166
        10         20         30         40         50         60 
MKSLLLLVLI SICWADHLSD NYTLDHDRAI HIQAENGPHL LVEAEQAKVF SHRGGNVTLP 

        70         80         90        100        110        120 
CKFYRDPTAF GSGIHKIRIK WTKLTSDYLK EVDVFVSMGY HKKTYGGYQG RVFLKGGSDS 

       130        140        150        160        170        180 
DASLVITDLT LEDYGRYKCE VIEGLEDDTV VVALDLQGVV FPYFPRLGRY NLNFHEAQQA 

       190        200        210        220        230        240 
CLDQDAVIAS FDQLYDAWRG GLDWCNAGWL SDGSVQYPIT KPREPCGGQN TVPGVRNYGF 

       250        260        270        280        290        300 
WDKDKSRYDV FCFTSNFNGR FYYLIHPTKL TYDEAVQACL NDGAQIAKVG QIFAAWKILG 

       310        320        330        340        350 
YDRCDAGWLA DGSVRYPISR PRRRCSPTEA AVRFVGFPDK KHKLYGVYCF RAYN 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of human cartilage link protein."
Dudhia J., Hardingham T.E.
Nucleic Acids Res. 18:1292-1292(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Articular chondrocyte.
[2]Erratum
Dudhia J., Hardingham T.E.
Nucleic Acids Res. 18:2214-2214(1990) [PubMed] [Europe PMC] [Abstract]
[3]"Complete amino acid sequence of human cartilage link protein (CRTL1) deduced from cDNA clones and chromosomal assignment of the gene."
Osborne-Lawrence S.L., Sinclair A.K., Hicks R.C., Lacey S.W., Eddy R.L. Jr., Byers M.G., Shows T.B., Duby A.D.
Genomics 8:562-567(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Characterization of the promoter for the rat and human link protein gene."
Rhodes C., Savagner P., Line S., Sasaki M., Chirigos M., Doege K., Yamada Y.
Nucleic Acids Res. 19:1933-1939(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[8]"The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain."
Hirakawa S., Oohashi T., Su W.-D., Yoshioka H., Murakami T., Arata J., Ninomiya Y.
Biochem. Biophys. Res. Commun. 276:982-989(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"A hyaluronan binding link protein gene family whose members are physically linked adjacent to chondroitin sulfate proteoglycan core protein genes: the missing links."
Spicer A.P., Joo A., Bowling R.A. Jr.
J. Biol. Chem. 278:21083-21091(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-333.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17405 mRNA. Translation: CAA35462.1.
U43328 mRNA. Translation: AAA85216.1.
AK313713 mRNA. Translation: BAG36456.1.
CH471084 Genomic DNA. Translation: EAW95912.1.
BC057808 mRNA. Translation: AAH57808.1.
CCDSCCDS4061.1.
PIRLKHU. S14914.
RefSeqNP_001875.1. NM_001884.3.
UniGeneHs.2799.

3D structure databases

ProteinModelPortalP10915.
SMRP10915. Positions 48-162, 166-253, 269-353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107794. 3 interactions.
STRING9606.ENSP00000274341.

PTM databases

PhosphoSiteP10915.

Polymorphism databases

DMDM130310.

Proteomic databases

MaxQBP10915.
PaxDbP10915.
PRIDEP10915.

Protocols and materials databases

DNASU1404.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274341; ENSP00000274341; ENSG00000145681.
GeneID1404.
KEGGhsa:1404.
UCSCuc003kim.3. human.

Organism-specific databases

CTD1404.
GeneCardsGC05M082933.
HGNCHGNC:2380. HAPLN1.
HPAHPA019105.
HPA019482.
MIM115435. gene.
neXtProtNX_P10915.
PharmGKBPA26901.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145467.
HOGENOMHOG000234353.
HOVERGENHBG051922.
InParanoidP10915.
KOK06848.
OMAWRSGLDW.
PhylomeDBP10915.
TreeFamTF332134.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.

Gene expression databases

ArrayExpressP10915.
BgeeP10915.
CleanExHS_HAPLN1.
GenevestigatorP10915.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.10.100.10. 2 hits.
InterProIPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
IPR000538. Link.
[Graphical view]
PfamPF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSPR01265. LINKMODULE.
SMARTSM00406. IGv. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMSSF56436. SSF56436. 2 hits.
PROSITEPS50835. IG_LIKE. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiHAPLN1.
GenomeRNAi1404.
NextBio5743.
PMAP-CutDBP10915.
PROP10915.
SOURCESearch...

Entry information

Entry nameHPLN1_HUMAN
AccessionPrimary (citable) accession number: P10915
Secondary accession number(s): B2R9A9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM