Reviewed,
UniProtKB/Swiss-Prot P10914 (IRF1_HUMAN)
Last modified
June 16, 2009.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Interferon regulatory factor 1 Short name=IRF-1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 325 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor. Ref.7 Ref.8 |
| Subcellular location | |
| Induction | By viruses and IFN. |
| Post-translational modification | Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 By similarity. Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites. Ubiquitinated. Appears to compete with sumoylation on C-terminal sites. Ref.7 |
| Involvement in disease | Deletion or rearrangement of IRF1 are a cause of preleukemic myelodysplastic syndrome (MDS) and of acute myelogenous leukemia (AML). |
| Sequence similarities | Belongs to the IRF family. Contains 1 tryptophan pentad repeat DNA-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle Transcription Transcription regulation |
| Cellular component | Nucleus |
| Ligand | DNA-binding |
| Molecular function | Activator Anti-oncogene |
| PTM | Isopeptide bond Ubl conjugation |
| Gene Ontology (GO) | |
| Biological process | cell cycle Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell cycleInferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoterTraceable author statement. Source: ProtInc |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 325 | 325 | Interferon regulatory factor 1 | PRO_0000154545 | |||||
Regions | |||||||||
| DNA binding | 7 – 109 | 103 | Tryptophan pentad repeat | ||||||
Amino acid modifications | |||||||||
| Cross-link | 275 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.7 | |||||||
| Cross-link | 299 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.7 | |||||||
Experimental info | |||||||||
| Mutagenesis | 275 | 1 | K → R: Some loss of sumoylation. Abolishes sumoylation, diminished ubiquitination, higher resistance to degradation, and increased apoptopic activity; when associated with R-299. Ref.7 | ||||||
| Mutagenesis | 299 | 1 | K → R: Large loss of sumoylation. Abolishes sumoylation, diminished ubiquitination, higher resistance to degradation, and increased apoptopic activity; when associated with R-275. Ref.7 | ||||||
| Sequence conflict | 5 | 1 | R → W Ref.1 | ||||||
| Sequence conflict | 5 | 1 | R → W Ref.2 | ||||||
| Sequence conflict | 34 – 35 | 2 | FQ → LE Ref.1 | ||||||
| Sequence conflict | 34 – 35 | 2 | FQ → LE Ref.2 | ||||||
| Sequence conflict | 220 | 1 | T → I Ref.1 | ||||||
| Sequence conflict | 220 | 1 | T → I Ref.2 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Sequence of a cDNA coding for human IRF-1." Maruyama M., Fujita T., Taniguchi T. Nucleic Acids Res. 17:3292-3292(1989) [PubMed: 2726461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements." Miyamoto M., Fujita T., Kimura Y., Maruyama M., Harada H., Sudo Y., Miyata T., Taniguchi T. Cell 54:903-913(1988) [PubMed: 3409321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Human interferon regulatory factor 1: intron-exon organization." Cha Y., Sims S.H., Romine M.F., Kaufmann M., Deisseroth A.B. DNA Cell Biol. 11:605-611(1992) [PubMed: 1382447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta. |
| [4] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas. |
| [6] | "Deletion of IRF-1, mapping to chromosome 5q31.1, in human leukemia and preleukemic myelodysplasia." Willman C.L., Sever C.E., Pallavicini M.G., Harada H., Tanaka N., Slovak M.L., Yamamoto H., Harada K., Meeker T.C., List A.F., Taniguchi T. Science 259:968-971(1993) [PubMed: 8438156] [Abstract] Cited for: INVOLVEMENT IN LEUKEMIAS. |
| [7] | "Elevated level of SUMOylated IRF-1 in tumor cells interferes with IRF-1-mediated apoptosis." Park J., Kim K., Lee E.-J., Seo Y.-J., Lim S.-N., Park K., Rho S.B., Lee S.-H., Lee J.-H. Proc. Natl. Acad. Sci. U.S.A. 104:17028-17033(2007) [PubMed: 17942705] [Abstract] Cited for: SUMOYLATION AT LYS-275 AND LYS-299, UBIQUITINATION AT LYS-275 AND LYS-299, FUNCTION, MUTAGENESIS OF LYS-275 AND LYS-299. |
| [8] | "IRF-1 promotes apoptosis in p53-damaged basal-type human mammary epithelial cells: a model for early basal-type mammary carcinogenesis." Bowie M.L., Ibarra C., Seewalt V.L. Adv. Exp. Med. Biol. 617:367-374(2008) [PubMed: 18497060] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X14454 mRNA. Translation: CAA32624.1. L05072 Genomic DNA. Translation: AAA36043.1. BT019756 mRNA. Translation: AAV38561.1. BC009483 mRNA. Translation: AAH09483.1. | |
| IPI | IPI00023600. |
| PIR | B31595. I52998. |
| RefSeq | NP_002189.1. |
| UniGene | Hs.436061 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IF1 based on UniProtKB P15314. |
| SMR | P10914. Positions 7-111. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P10914. 2 interactions. |
PTM databases | |
| PhosphoSite | P10914. |
Proteomic databases | |
| PRIDE | P10914. |
Genome annotation databases | |
| Ensembl | ENSG00000125347. Homo sapiens. [Contig view] |
| GeneID | 3659. |
| KEGG | hsa:3659. |
Organism-specific databases | |
| GeneCards | GC05M131846. |
| H-InvDB | HIX0005159. |
| HGNC | HGNC:6116. IRF1. |
| HPA | CAB011662. |
| MIM | 147575. gene. 153550. phenotype. |
| PharmGKB | PA29915. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P10914. |
| HOVERGEN | P10914. |
| OMA | P10914. CKEEPEV. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | ifngpathway. IFN-gamma pathway. il12_stat4pathway. IL12 signaling mediated by STAT4. il6_7pathway. IL6-mediated signaling events. telomerasepathway. Regulation of Telomerase. |
Gene expression databases | |
| ArrayExpress | P10914. |
| Bgee | P10914. |
| CleanEx | HS_IRF1. |
| GermOnline | ENSG00000125347. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR017431. Interferon_regulatory_fac-1/2. IPR019817. Interferon_regulatory_fac_CS. IPR001346. Interferon_regulatory_factor. IPR011991. Wing_hlx_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF00605. IRF. 1 hit. [Graphical view] |
| PIRSF | PIRSF038196. IFN_RF1/2. 1 hit. |
| PRINTS | PR00267. INTFRNREGFCT. |
| ProDom | PD002355. IRF. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00348. IRF. 1 hit. [Graphical view] |
| PROSITE | PS00601. IRF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 14311. |
| SOURCE | Search... |
Entry information
| Entry name | IRF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P10914 Secondary accession number(s): Q96GG7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


